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The map label for this gene is thiC

Identifier: 58617024

GI number: 58617024

Start: 495756

End: 497423

Strand: Reverse

Name: thiC

Synonym: ERGA_CDS_02970

Alternate gene names: 58617024

Gene position: 497423-495756 (Counterclockwise)

Preceding gene: 58617025

Following gene: 58617022

Centisome position: 33.16

GC content: 31.95

Gene sequence:

>1668_bases
ATGAAAATTGACCTTAATACTATATTCCCATCTTCATGTAAAGAATACATACCAGGTAAAATCTATAAAAATATTAAAAT
AGGTATGCGCAAAGTCTCTTTCAACAATGATACTGAATCAATCTTAATATATGATACCGGAGGACCACATTCTGATCAAG
ATATACAAACCAATATAAACAATGGTATTAAAAAATTACGGGTTAATTGGATAACAGATAGGCAAGATGTAGAATATTAT
GATAGACATACAATCAATACAAATTCCAGTACAGCATTTCCTTTACAAAATAACAAAGCTTTAAAATCCAAAAATGATAA
ACCTGTAACTCAGATGTTTTACGCAAAAAATAATATTATTACTCCAGAAATGGAATATGTTGCAATACGAGAAAACTCTT
TAATACAAAAACTTCTATCACACACACCAAATACCATAATTCCAGAAATAACTCCAGAACTTGTGAGACAAGAAGTAGCT
GCTGGTCGAGCAATTATTCCTGCAAATATTAATCATCCAGAATCAGAACCTATGATTATTGGTAAGAACTTCCTAGTAAA
AATCAATGCCAATATTGGAAACTCAGCAGTAAGTTCAGACATAAATAATGAAGTATATAAAATGATATATGCAATCATAT
ATGGTGCAGATACAGTAATGGATTTATCAACAGGAAGTCATATACATAATACTAGAGAATGGATTATTAGGAACAGCCCT
GTACCAATAGGTACAGTACCTATATACCAAGCATTGAATAAAGTAAATGGAATAGTAGGAGAACTTAATTTTAATATTTT
TAAAGAAACACTAATTGAACAAGCAGAACAAGGAGTGGATTATTTTACTATACATGCTGGTGTATTAAAAAAATACATTC
AATACACAACCAACCGGCTAACTGGCATTGTATCTAGAGGTGGAGCTATTATTGCACAATGGTGTTCTATACATAATAAA
GAGAACTTTCTCTATACAAATTTTGAGGAAATATGTGATATAATGAAAAGTTACGATATTGCTTTCTCTTTAGGAGATGG
TCTAAGACCAGGTTCTATAGCGGATGCAAACGATAAAGCACAGTTTTTAGAATTAAAGACACTAGGAGAACTTACAGATA
TTGCATGGAAACATGATTGTCAAGTTATGATAGAAGGTCCAGGACATGTACCAATGCATTTAATAAAAGAAAATGTAGAA
AAACAGATGTACTTTTGTAAAGAAGCCCCATTTTATACTTTAGGACCACTCACTACAGACATTGCTCCTGGATATGATCA
CATAACTAGTGCCATAGGTGCAGCAATGATAGGATGGTATGGTACTTCTATGTTATGTTATGTCACACCAAAAGAACACC
TTGGATTACCAAATCTCAATGATGTAAAAAATGGAGTAATTACATATAAAATCGCAGCACATGCTGCTGATCTAGCAAAA
GGTAATCCTTCCGCTTATATAAGAGATTATGCATTAAGTTATGCAAGATTTAATTTTAGATGGTACGATCAATTCAACTT
ATCACTTGATCCAGAAACAGCAAAATCTTTTCACGATGAATCTCTACCTTCTGAACATGCAAAATCTGCACATTTTTGTT
CAATGTGCGGACCAAAATTTTGCTCAATGAAATTAACACATCAACTACAACTTAACTCTACTGAATAA

Upstream 100 bases:

>100_bases
TATTTACATATTTTATATTAACTTTAATCTTTTTACAATTATAGACATCAAATGAAAATTACATATACTATATTTGTATA
TATCATTTGACTAATTAAGT

Downstream 100 bases:

>100_bases
ACAAAGGTCTAACAATACAATACCTTTAATTACAAATTTTATAATAGCCATGCTCATAATTTAATATACAAGACACATAT
AATATAAAACTTTTCATTCT

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 555; Mature: 555

Protein sequence:

>555_residues
MKIDLNTIFPSSCKEYIPGKIYKNIKIGMRKVSFNNDTESILIYDTGGPHSDQDIQTNINNGIKKLRVNWITDRQDVEYY
DRHTINTNSSTAFPLQNNKALKSKNDKPVTQMFYAKNNIITPEMEYVAIRENSLIQKLLSHTPNTIIPEITPELVRQEVA
AGRAIIPANINHPESEPMIIGKNFLVKINANIGNSAVSSDINNEVYKMIYAIIYGADTVMDLSTGSHIHNTREWIIRNSP
VPIGTVPIYQALNKVNGIVGELNFNIFKETLIEQAEQGVDYFTIHAGVLKKYIQYTTNRLTGIVSRGGAIIAQWCSIHNK
ENFLYTNFEEICDIMKSYDIAFSLGDGLRPGSIADANDKAQFLELKTLGELTDIAWKHDCQVMIEGPGHVPMHLIKENVE
KQMYFCKEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWYGTSMLCYVTPKEHLGLPNLNDVKNGVITYKIAAHAADLAK
GNPSAYIRDYALSYARFNFRWYDQFNLSLDPETAKSFHDESLPSEHAKSAHFCSMCGPKFCSMKLTHQLQLNSTE

Sequences:

>Translated_555_residues
MKIDLNTIFPSSCKEYIPGKIYKNIKIGMRKVSFNNDTESILIYDTGGPHSDQDIQTNINNGIKKLRVNWITDRQDVEYY
DRHTINTNSSTAFPLQNNKALKSKNDKPVTQMFYAKNNIITPEMEYVAIRENSLIQKLLSHTPNTIIPEITPELVRQEVA
AGRAIIPANINHPESEPMIIGKNFLVKINANIGNSAVSSDINNEVYKMIYAIIYGADTVMDLSTGSHIHNTREWIIRNSP
VPIGTVPIYQALNKVNGIVGELNFNIFKETLIEQAEQGVDYFTIHAGVLKKYIQYTTNRLTGIVSRGGAIIAQWCSIHNK
ENFLYTNFEEICDIMKSYDIAFSLGDGLRPGSIADANDKAQFLELKTLGELTDIAWKHDCQVMIEGPGHVPMHLIKENVE
KQMYFCKEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWYGTSMLCYVTPKEHLGLPNLNDVKNGVITYKIAAHAADLAK
GNPSAYIRDYALSYARFNFRWYDQFNLSLDPETAKSFHDESLPSEHAKSAHFCSMCGPKFCSMKLTHQLQLNSTE
>Mature_555_residues
MKIDLNTIFPSSCKEYIPGKIYKNIKIGMRKVSFNNDTESILIYDTGGPHSDQDIQTNINNGIKKLRVNWITDRQDVEYY
DRHTINTNSSTAFPLQNNKALKSKNDKPVTQMFYAKNNIITPEMEYVAIRENSLIQKLLSHTPNTIIPEITPELVRQEVA
AGRAIIPANINHPESEPMIIGKNFLVKINANIGNSAVSSDINNEVYKMIYAIIYGADTVMDLSTGSHIHNTREWIIRNSP
VPIGTVPIYQALNKVNGIVGELNFNIFKETLIEQAEQGVDYFTIHAGVLKKYIQYTTNRLTGIVSRGGAIIAQWCSIHNK
ENFLYTNFEEICDIMKSYDIAFSLGDGLRPGSIADANDKAQFLELKTLGELTDIAWKHDCQVMIEGPGHVPMHLIKENVE
KQMYFCKEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWYGTSMLCYVTPKEHLGLPNLNDVKNGVITYKIAAHAADLAK
GNPSAYIRDYALSYARFNFRWYDQFNLSLDPETAKSFHDESLPSEHAKSAHFCSMCGPKFCSMKLTHQLQLNSTE

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=579, Percent_Identity=59.5854922279793, Blast_Score=716, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_EHRRG (Q5FHG9)

Other databases:

- EMBL:   CR925677
- RefSeq:   YP_196223.1
- STRING:   Q5FHG9
- GeneID:   3268783
- GenomeReviews:   CR925677_GR
- KEGG:   erg:ERGA_CDS_02970
- eggNOG:   COG0422
- HOGENOM:   HBG285234
- OMA:   TWELFRD
- ProtClustDB:   PRK09284
- BioCyc:   ERUM302409:ERGA_CDS_02970-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 62385; Mature: 62385

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: BINDING 191-191 BINDING 220-220 BINDING 249-249 BINDING 285-285 BINDING 385-385 BINDING 412-412

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIDLNTIFPSSCKEYIPGKIYKNIKIGMRKVSFNNDTESILIYDTGGPHSDQDIQTNIN
CEEECCCCCCHHHHHHCCCHHHHHHEEEEEEEECCCCCCEEEEEECCCCCCCCCHHHHHH
NGIKKLRVNWITDRQDVEYYDRHTINTNSSTAFPLQNNKALKSKNDKPVTQMFYAKNNII
CCCEEEEEEEECCCCCCHHHHCEEECCCCCEEECCCCCCHHCCCCCCCHHHHHHHCCCCC
TPEMEYVAIRENSLIQKLLSHTPNTIIPEITPELVRQEVAAGRAIIPANINHPESEPMII
CCCCEEEEECCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCCEEE
GKNFLVKINANIGNSAVSSDINNEVYKMIYAIIYGADTVMDLSTGSHIHNTREWIIRNSP
CCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHEEEECCC
VPIGTVPIYQALNKVNGIVGELNFNIFKETLIEQAEQGVDYFTIHAGVLKKYIQYTTNRL
CCCCCHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHH
TGIVSRGGAIIAQWCSIHNKENFLYTNFEEICDIMKSYDIAFSLGDGLRPGSIADANDKA
HHHHHCCCHHHHHHHHHCCCCCEEEECHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCH
QFLELKTLGELTDIAWKHDCQVMIEGPGHVPMHLIKENVEKQMYFCKEAPFYTLGPLTTD
HHEEHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCC
IAPGYDHITSAIGAAMIGWYGTSMLCYVTPKEHLGLPNLNDVKNGVITYKIAAHAADLAK
CCCCHHHHHHHHHHHHHHHHCCCEEEEECCHHHCCCCCCCHHCCCEEEEEEEHHHHHHHC
GNPSAYIRDYALSYARFNFRWYDQFNLSLDPETAKSFHDESLPSEHAKSAHFCSMCGPKF
CCCCHHHHHHHHHHHEEEEEEEEEECCEECCHHHHHHCCCCCCHHHHCCCHHHHHCCCCH
CSMKLTHQLQLNSTE
HCEEEEEEEEECCCC
>Mature Secondary Structure
MKIDLNTIFPSSCKEYIPGKIYKNIKIGMRKVSFNNDTESILIYDTGGPHSDQDIQTNIN
CEEECCCCCCHHHHHHCCCHHHHHHEEEEEEEECCCCCCEEEEEECCCCCCCCCHHHHHH
NGIKKLRVNWITDRQDVEYYDRHTINTNSSTAFPLQNNKALKSKNDKPVTQMFYAKNNII
CCCEEEEEEEECCCCCCHHHHCEEECCCCCEEECCCCCCHHCCCCCCCHHHHHHHCCCCC
TPEMEYVAIRENSLIQKLLSHTPNTIIPEITPELVRQEVAAGRAIIPANINHPESEPMII
CCCCEEEEECCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCCEEE
GKNFLVKINANIGNSAVSSDINNEVYKMIYAIIYGADTVMDLSTGSHIHNTREWIIRNSP
CCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHEEEECCC
VPIGTVPIYQALNKVNGIVGELNFNIFKETLIEQAEQGVDYFTIHAGVLKKYIQYTTNRL
CCCCCHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHH
TGIVSRGGAIIAQWCSIHNKENFLYTNFEEICDIMKSYDIAFSLGDGLRPGSIADANDKA
HHHHHCCCHHHHHHHHHCCCCCEEEECHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCH
QFLELKTLGELTDIAWKHDCQVMIEGPGHVPMHLIKENVEKQMYFCKEAPFYTLGPLTTD
HHEEHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCC
IAPGYDHITSAIGAAMIGWYGTSMLCYVTPKEHLGLPNLNDVKNGVITYKIAAHAADLAK
CCCCHHHHHHHHHHHHHHHHCCCEEEEECCHHHCCCCCCCHHCCCEEEEEEEHHHHHHHC
GNPSAYIRDYALSYARFNFRWYDQFNLSLDPETAKSFHDESLPSEHAKSAHFCSMCGPKF
CCCCHHHHHHHHHHHEEEEEEEEEECCEECCHHHHHHCCCCCCHHHHCCCHHHHHCCCCH
CSMKLTHQLQLNSTE
HCEEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA