Click here to switch to the map view.

The map label for this gene is birA [H]

Identifier: 58616979

GI number: 58616979

Start: 414569

End: 415327

Strand: Reverse

Name: birA [H]

Synonym: ERGA_CDS_02520

Alternate gene names: 58616979

Gene position: 415327-414569 (Counterclockwise)

Preceding gene: 58616980

Following gene: 58616978

Centisome position: 27.69

GC content: 27.4

Gene sequence:

>759_bases
ATGAAAACAATGCAACAATACATAAAACTACCTAGTGGATATTCCTTAGAACATCATGAAGTGTTATACAGCACAAATTA
TGAAGCAATAAAACTTGCACAATGTGGTGGTAATGATGGAACAATTATCATATCTGATAAACAAATAAAAGGAAAAGGTA
GAAACAATCGTACATGGTATTCTCCACAAGGAAATCTATATTTTAGTATTATAATAAGAGAGCTATCAGAAGTATTTCAT
TTACCATTTATCTCTGCATTATCTGTTGGTACAACACTTGAACAAATACTCAATAAAGAAAACAATACAAATGCAAAAGT
ACAATATAAATGGCCTAATGATATATTAGTGAATAGCAAAAAAATATCAGGAATATTATTAGAAAGTAATATAGAGCATA
ACAAATCTCAAGAATGGGTAGTAATTGGAATTGGAGTAAACATATACCATGCTCCAGAATATGCTACAACTATACAGAAT
AATATACCAAATATTAGTTTATCAAATTTGGAATTATTAGAAAAAATAATCTATAATTTTGATAAATTGCGAAAAGATTA
CTTAAACAATGGTTTTTCAAATATACGCAATTTATGGCTTAAGAATACATACCATAATTTTAACGACCAAATCACCATCC
AAAGTTGTAATAACATTTATGTAGGAAATTTTGTAACATTAGACTTGAATGGTAGTCTTGTTATAGAACAAGATGGACAA
GCTGTACATATTACTTCAGGCGAAATATTTAATATTTAA

Upstream 100 bases:

>100_bases
TAAAACGTAATATAAAAAAATATGTGTACAGCATATTATAAACTAGGTAATAAAAAATTATTCATTTATAGTATGAAGAC
TCTCACTATTAGTACATCAT

Downstream 100 bases:

>100_bases
ATAGCATAACACATCTCACAATCATTATAAAAACATATTAAACAGCCTACTATTATACTTTGAAAATTTGAAACCATGAT
ATATATGATTTACCAAGTAA

Product: biotin-protein ligase

Products: NA

Alternate protein names: Biotin--protein ligase [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MKTMQQYIKLPSGYSLEHHEVLYSTNYEAIKLAQCGGNDGTIIISDKQIKGKGRNNRTWYSPQGNLYFSIIIRELSEVFH
LPFISALSVGTTLEQILNKENNTNAKVQYKWPNDILVNSKKISGILLESNIEHNKSQEWVVIGIGVNIYHAPEYATTIQN
NIPNISLSNLELLEKIIYNFDKLRKDYLNNGFSNIRNLWLKNTYHNFNDQITIQSCNNIYVGNFVTLDLNGSLVIEQDGQ
AVHITSGEIFNI

Sequences:

>Translated_252_residues
MKTMQQYIKLPSGYSLEHHEVLYSTNYEAIKLAQCGGNDGTIIISDKQIKGKGRNNRTWYSPQGNLYFSIIIRELSEVFH
LPFISALSVGTTLEQILNKENNTNAKVQYKWPNDILVNSKKISGILLESNIEHNKSQEWVVIGIGVNIYHAPEYATTIQN
NIPNISLSNLELLEKIIYNFDKLRKDYLNNGFSNIRNLWLKNTYHNFNDQITIQSCNNIYVGNFVTLDLNGSLVIEQDGQ
AVHITSGEIFNI
>Mature_252_residues
MKTMQQYIKLPSGYSLEHHEVLYSTNYEAIKLAQCGGNDGTIIISDKQIKGKGRNNRTWYSPQGNLYFSIIIRELSEVFH
LPFISALSVGTTLEQILNKENNTNAKVQYKWPNDILVNSKKISGILLESNIEHNKSQEWVVIGIGVNIYHAPEYATTIQN
NIPNISLSNLELLEKIIYNFDKLRKDYLNNGFSNIRNLWLKNTYHNFNDQITIQSCNNIYVGNFVTLDLNGSLVIEQDGQ
AVHITSGEIFNI

Specific function: Activates biotin to form biotinyl-5'-adenylate and transfers the biotin moiety to biotin-accepting proteins [H]

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=217, Percent_Identity=28.5714285714286, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004408
- InterPro:   IPR003142
- InterPro:   IPR004143 [H]

Pfam domain/function: PF02237 BPL_C; PF03099 BPL_LipA_LipB [H]

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 28758; Mature: 28758

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTMQQYIKLPSGYSLEHHEVLYSTNYEAIKLAQCGGNDGTIIISDKQIKGKGRNNRTWY
CCCHHHHHCCCCCCCCCCCEEEEECCCCEEEEEECCCCCCEEEEECCEEECCCCCCCEEE
SPQGNLYFSIIIRELSEVFHLPFISALSVGTTLEQILNKENNTNAKVQYKWPNDILVNSK
CCCCCEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEECC
KISGILLESNIEHNKSQEWVVIGIGVNIYHAPEYATTIQNNIPNISLSNLELLEKIIYNF
CEEEEEEECCCCCCCCCCEEEEEECEEEEECCCHHHHHHCCCCCCEEHHHHHHHHHHHHH
DKLRKDYLNNGFSNIRNLWLKNTYHNFNDQITIQSCNNIYVGNFVTLDLNGSLVIEQDGQ
HHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEEEEECCCEEECCEEEEEECCEEEEECCCC
AVHITSGEIFNI
EEEEECCCEECC
>Mature Secondary Structure
MKTMQQYIKLPSGYSLEHHEVLYSTNYEAIKLAQCGGNDGTIIISDKQIKGKGRNNRTWY
CCCHHHHHCCCCCCCCCCCEEEEECCCCEEEEEECCCCCCEEEEECCEEECCCCCCCEEE
SPQGNLYFSIIIRELSEVFHLPFISALSVGTTLEQILNKENNTNAKVQYKWPNDILVNSK
CCCCCEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEECC
KISGILLESNIEHNKSQEWVVIGIGVNIYHAPEYATTIQNNIPNISLSNLELLEKIIYNF
CEEEEEEECCCCCCCCCCEEEEEECEEEEECCCHHHHHHCCCCCCEEHHHHHHHHHHHHH
DKLRKDYLNNGFSNIRNLWLKNTYHNFNDQITIQSCNNIYVGNFVTLDLNGSLVIEQDGQ
HHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEEEEECCCEEECCEEEEEECCEEEEECCCC
AVHITSGEIFNI
EEEEECCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8422400 [H]