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The map label for this gene is purB [H]

Identifier: 58616973

GI number: 58616973

Start: 399555

End: 400853

Strand: Reverse

Name: purB [H]

Synonym: ERGA_CDS_02460

Alternate gene names: 58616973

Gene position: 400853-399555 (Counterclockwise)

Preceding gene: 58616976

Following gene: 58616968

Centisome position: 26.72

GC content: 30.64

Gene sequence:

>1299_bases
ATGATCAAACGCTATAGCAGACCTGAAATGTCAGCAATCTGGGAAGAAAACAATAAATTTAAAATATGGATGGATATAGA
ATATTATGCATGTGAAGCACAAGTAAAACTCGGTATTATTCCAAGTTCTATACTTGAGAATTTAAAAAATAAAATTGAAA
ACTTTGATATAGACCGAATTAATGAGATTGAATCTGTAGTAAAACATGATGTAATAGCATTTCTAACATATATAGCAGAA
TCAACCAATTCAGATATCAGATATCTACACTATGGCATGACAAGTTCTGACGTATTAGATACATGCCTCTCTATTCAACT
AAAACAATCTTGTGACCTACTACTATGTAACATAGAAGTTATACTAAATATTCTAAGGGAAAAATCCATAGAAACAAAAT
ACATGCTATGTATGGGAAGAAGTCATGGCATACATGCAGAACCAATCACATTTGGCTTAAAACTTGCAAGATTTTATGCA
GAGTTTAAACGTAACTACAACAGATTAAAAATCGCAAAAGATGAAATATCAGTATGTAAAATTTCAGGAGCGATGGGTAA
CTTTGCTAATATTGATCCATTTGTTGAAGAATATGTAGCAAATTCCTTAGGTTTAAAATCCGAGTCCATATCTTCACAAG
TCATACCAAGAGATCGACATGCAATGTTTTTTTCAACATTAGGAGTAATTGCATCCTCTATAGAAAACCTAGCAATAGAA
ATTCGTCACCTACAAAGAACTGAACTAATGGAAGCAATAGAATATTTTTCATCAGGGCAAAAAGGTAGCTCAGCAATGCC
TCATAAAAAAAATCCCATATTTAGTGAAAATTTGACAGGACTTTCTAGAATAATACGCAGTTATGTAATCCCTGCACTAG
AAAACGTTGCACTATGGCATGAAAGAGACATATCTCACTCTTCTGTTGAACGATGTATTGCACCAGATGCTTGTATTACT
ATGGATTTTGCATTAGTTAGGTTGGCAAATTTACTTCAAAATATAATTTTATCTCCAGACAATATGAAGAAAAATATAGA
TCTATCTAAAGGCTTAGTATTTTCTCAAAGAATACTATTAGAACTAGTGAAACATGGACTAACAAGAGAAAAAGCATATG
AAATTATTCAAAGTAGAGCATTGCAAGTATGGGATAACAACAGTAACTTTTTAGATGAACTAAAAAATGATCCTCAAGTA
GCAAAATATATAGATAATAAAGAATTAGAATCACTATTTAATTTTAATTACTATACCAAACATATAGATAAAATATTCGA
GAAAGTATTCAATGAATAA

Upstream 100 bases:

>100_bases
AATTATAATAATGCTTTACACATAACATGTTATATATATTTTTACACAAGCAATAATATTTTAACGTTCGCAAAATAATT
AAAAATATTGGAGATAAATA

Downstream 100 bases:

>100_bases
ATCTTATATATACATACCACAAGATAACAATATTTATTATATTTATATAATAAAACTAATTTTTACATTATTTGAACTTG
TATTTTTGTATGATAATAAT

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MIKRYSRPEMSAIWEENNKFKIWMDIEYYACEAQVKLGIIPSSILENLKNKIENFDIDRINEIESVVKHDVIAFLTYIAE
STNSDIRYLHYGMTSSDVLDTCLSIQLKQSCDLLLCNIEVILNILREKSIETKYMLCMGRSHGIHAEPITFGLKLARFYA
EFKRNYNRLKIAKDEISVCKISGAMGNFANIDPFVEEYVANSLGLKSESISSQVIPRDRHAMFFSTLGVIASSIENLAIE
IRHLQRTELMEAIEYFSSGQKGSSAMPHKKNPIFSENLTGLSRIIRSYVIPALENVALWHERDISHSSVERCIAPDACIT
MDFALVRLANLLQNIILSPDNMKKNIDLSKGLVFSQRILLELVKHGLTREKAYEIIQSRALQVWDNNSNFLDELKNDPQV
AKYIDNKELESLFNFNYYTKHIDKIFEKVFNE

Sequences:

>Translated_432_residues
MIKRYSRPEMSAIWEENNKFKIWMDIEYYACEAQVKLGIIPSSILENLKNKIENFDIDRINEIESVVKHDVIAFLTYIAE
STNSDIRYLHYGMTSSDVLDTCLSIQLKQSCDLLLCNIEVILNILREKSIETKYMLCMGRSHGIHAEPITFGLKLARFYA
EFKRNYNRLKIAKDEISVCKISGAMGNFANIDPFVEEYVANSLGLKSESISSQVIPRDRHAMFFSTLGVIASSIENLAIE
IRHLQRTELMEAIEYFSSGQKGSSAMPHKKNPIFSENLTGLSRIIRSYVIPALENVALWHERDISHSSVERCIAPDACIT
MDFALVRLANLLQNIILSPDNMKKNIDLSKGLVFSQRILLELVKHGLTREKAYEIIQSRALQVWDNNSNFLDELKNDPQV
AKYIDNKELESLFNFNYYTKHIDKIFEKVFNE
>Mature_432_residues
MIKRYSRPEMSAIWEENNKFKIWMDIEYYACEAQVKLGIIPSSILENLKNKIENFDIDRINEIESVVKHDVIAFLTYIAE
STNSDIRYLHYGMTSSDVLDTCLSIQLKQSCDLLLCNIEVILNILREKSIETKYMLCMGRSHGIHAEPITFGLKLARFYA
EFKRNYNRLKIAKDEISVCKISGAMGNFANIDPFVEEYVANSLGLKSESISSQVIPRDRHAMFFSTLGVIASSIENLAIE
IRHLQRTELMEAIEYFSSGQKGSSAMPHKKNPIFSENLTGLSRIIRSYVIPALENVALWHERDISHSSVERCIAPDACIT
MDFALVRLANLLQNIILSPDNMKKNIDLSKGLVFSQRILLELVKHGLTREKAYEIIQSRALQVWDNNSNFLDELKNDPQV
AKYIDNKELESLFNFNYYTKHIDKIFEKVFNE

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=390, Percent_Identity=26.4102564102564, Blast_Score=138, Evalue=8e-33,
Organism=Homo sapiens, GI183227688, Length=383, Percent_Identity=26.3707571801567, Blast_Score=135, Evalue=6e-32,
Organism=Escherichia coli, GI1787376, Length=305, Percent_Identity=25.5737704918033, Blast_Score=85, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17508577, Length=395, Percent_Identity=26.3291139240506, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI32564234, Length=352, Percent_Identity=26.9886363636364, Blast_Score=102, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6323391, Length=392, Percent_Identity=24.4897959183673, Blast_Score=127, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24647570, Length=452, Percent_Identity=23.4513274336283, Blast_Score=109, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 49706; Mature: 49706

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKRYSRPEMSAIWEENNKFKIWMDIEYYACEAQVKLGIIPSSILENLKNKIENFDIDRI
CCCCCCCCHHHHHHCCCCEEEEEEEEEEEEEEEEEEEECCHHHHHHHHHHHHHCCCCHHH
NEIESVVKHDVIAFLTYIAESTNSDIRYLHYGMTSSDVLDTCLSIQLKQSCDLLLCNIEV
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCEEEECHHH
ILNILREKSIETKYMLCMGRSHGIHAEPITFGLKLARFYAEFKRNYNRLKIAKDEISVCK
HHHHHHHHCCCHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCHHEEE
ISGAMGNFANIDPFVEEYVANSLGLKSESISSQVIPRDRHAMFFSTLGVIASSIENLAIE
ECCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
IRHLQRTELMEAIEYFSSGQKGSSAMPHKKNPIFSENLTGLSRIIRSYVIPALENVALWH
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ERDISHSSVERCIAPDACITMDFALVRLANLLQNIILSPDNMKKNIDLSKGLVFSQRILL
HCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
ELVKHGLTREKAYEIIQSRALQVWDNNSNFLDELKNDPQVAKYIDNKELESLFNFNYYTK
HHHHHCCCHHHHHHHHHHHHHHEECCCCHHHHHHCCCCHHHHHHCHHHHHHHHCCCHHHH
HIDKIFEKVFNE
HHHHHHHHHHCC
>Mature Secondary Structure
MIKRYSRPEMSAIWEENNKFKIWMDIEYYACEAQVKLGIIPSSILENLKNKIENFDIDRI
CCCCCCCCHHHHHHCCCCEEEEEEEEEEEEEEEEEEEECCHHHHHHHHHHHHHCCCCHHH
NEIESVVKHDVIAFLTYIAESTNSDIRYLHYGMTSSDVLDTCLSIQLKQSCDLLLCNIEV
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCEEEECHHH
ILNILREKSIETKYMLCMGRSHGIHAEPITFGLKLARFYAEFKRNYNRLKIAKDEISVCK
HHHHHHHHCCCHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCHHEEE
ISGAMGNFANIDPFVEEYVANSLGLKSESISSQVIPRDRHAMFFSTLGVIASSIENLAIE
ECCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
IRHLQRTELMEAIEYFSSGQKGSSAMPHKKNPIFSENLTGLSRIIRSYVIPALENVALWH
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ERDISHSSVERCIAPDACITMDFALVRLANLLQNIILSPDNMKKNIDLSKGLVFSQRILL
HCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
ELVKHGLTREKAYEIIQSRALQVWDNNSNFLDELKNDPQVAKYIDNKELESLFNFNYYTK
HHHHHCCCHHHHHHHHHHHHHHEECCCCHHHHHHCCCCHHHHHHCHHHHHHHHCCCHHHH
HIDKIFEKVFNE
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]