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The map label for this gene is thiD [H]

Identifier: 58616914

GI number: 58616914

Start: 307882

End: 308682

Strand: Reverse

Name: thiD [H]

Synonym: ERGA_CDS_01870

Alternate gene names: 58616914

Gene position: 308682-307882 (Counterclockwise)

Preceding gene: 58616915

Following gene: 58616907

Centisome position: 20.58

GC content: 31.59

Gene sequence:

>801_bases
ATGAGCCAATACAAAGGAAAAGTATTAACTATAGCAGGATCTGACTCCAGTGGAGGAGCAGGTATTCAAGCAGACATAAA
GACAATATCATCTCTAGGGTGCTATGCTGCAAGTTGTATTACATCTGTAACTGTTCAAAATACAATGCAGGTTTATCAAG
TACACAATATACCACAGGATATCATAATAGGACAAATAGAAGCTGTTTTATCTGATATAACAATAGATGCAATAAAAATT
GGTATGTTACAATCCCACGATGTTATAACAGCAGTAGCAAATTCACTCCCATCAAATATTCCTATAATCGTAGATCCAGT
AATGATATCAACATCAAATTTTAAGTTAATGAATGATAGTTCAGTATTTCACTTTATAGAACACATACTTCCAAAAGCAA
CAGTAATTACACCAAACATATTGGAAGCAGAAATTTTAACTAACACAAAAATCATAGATGAGTACTGTATGAAACAAGCA
AGTAAAATACTTAAATCACTTGGTACAAAATCTGTTTTAATCAAAGGTGGACACTTAAATCGTGAAACTATTGATAATAT
TTTTTTAACAGAAGATGATCAAATATTCAATTTTTCCCACCACAGAATTCCACACAATGAACTACATGGAACAGGATGCA
CTTTATCTTCAGCTATTGCAAGTTTTGTTTCACAAAAAATCCCTATATCAGACAGTATAGACTTGGCAATACAATACCTT
CTCAATACTATCAAGACAATACCAAAAGTAGGAAAAGGTCACAATCCAGTTTTTCACAATTACAATATTATAAGTAACTA
A

Upstream 100 bases:

>100_bases
TTTCAAAGTTTAAGTAATATTGTAAAATGCATATACCGAGAATTACCATATTACAACAATGGAGTAACCATACCATTTGT
AAAATCTATAATAGGAAAAA

Downstream 100 bases:

>100_bases
TCTTTTTCATGTACTATGTATGTAAGTATAGTGATCACTCACTTTTAACTAATACTGTTATCAAACCTATTATTTAAGCT
AAAACACCACATATTATAAC

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Thiamine-phosphate pyrophosphorylase; TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase; Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MSQYKGKVLTIAGSDSSGGAGIQADIKTISSLGCYAASCITSVTVQNTMQVYQVHNIPQDIIIGQIEAVLSDITIDAIKI
GMLQSHDVITAVANSLPSNIPIIVDPVMISTSNFKLMNDSSVFHFIEHILPKATVITPNILEAEILTNTKIIDEYCMKQA
SKILKSLGTKSVLIKGGHLNRETIDNIFLTEDDQIFNFSHHRIPHNELHGTGCTLSSAIASFVSQKIPISDSIDLAIQYL
LNTIKTIPKVGKGHNPVFHNYNIISN

Sequences:

>Translated_266_residues
MSQYKGKVLTIAGSDSSGGAGIQADIKTISSLGCYAASCITSVTVQNTMQVYQVHNIPQDIIIGQIEAVLSDITIDAIKI
GMLQSHDVITAVANSLPSNIPIIVDPVMISTSNFKLMNDSSVFHFIEHILPKATVITPNILEAEILTNTKIIDEYCMKQA
SKILKSLGTKSVLIKGGHLNRETIDNIFLTEDDQIFNFSHHRIPHNELHGTGCTLSSAIASFVSQKIPISDSIDLAIQYL
LNTIKTIPKVGKGHNPVFHNYNIISN
>Mature_265_residues
SQYKGKVLTIAGSDSSGGAGIQADIKTISSLGCYAASCITSVTVQNTMQVYQVHNIPQDIIIGQIEAVLSDITIDAIKIG
MLQSHDVITAVANSLPSNIPIIVDPVMISTSNFKLMNDSSVFHFIEHILPKATVITPNILEAEILTNTKIIDEYCMKQAS
KILKSLGTKSVLIKGGHLNRETIDNIFLTEDDQIFNFSHHRIPHNELHGTGCTLSSAIASFVSQKIPISDSIDLAIQYLL
NTIKTIPKVGKGHNPVFHNYNIISN

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=262, Percent_Identity=38.9312977099237, Blast_Score=172, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6325378, Length=277, Percent_Identity=33.5740072202166, Blast_Score=140, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6324517, Length=275, Percent_Identity=31.2727272727273, Blast_Score=123, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6324997, Length=277, Percent_Identity=31.4079422382672, Blast_Score=122, Evalue=6e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013749
- InterPro:   IPR004399
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF08543 Phos_pyr_kin; PF02581 TMP-TENI [H]

EC number: =2.5.1.3; =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 28909; Mature: 28778

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQYKGKVLTIAGSDSSGGAGIQADIKTISSLGCYAASCITSVTVQNTMQVYQVHNIPQD
CCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
IIIGQIEAVLSDITIDAIKIGMLQSHDVITAVANSLPSNIPIIVDPVMISTSNFKLMNDS
HHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHCCCCCCEEECCEEEECCCEEEECCC
SVFHFIEHILPKATVITPNILEAEILTNTKIIDEYCMKQASKILKSLGTKSVLIKGGHLN
HHHHHHHHHCCCCEEECCCHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCC
RETIDNIFLTEDDQIFNFSHHRIPHNELHGTGCTLSSAIASFVSQKIPISDSIDLAIQYL
HHHHCCEEECCCCCEECCCCCCCCCHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHH
LNTIKTIPKVGKGHNPVFHNYNIISN
HHHHHHHHCCCCCCCCCEECCEECCC
>Mature Secondary Structure 
SQYKGKVLTIAGSDSSGGAGIQADIKTISSLGCYAASCITSVTVQNTMQVYQVHNIPQD
CCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
IIIGQIEAVLSDITIDAIKIGMLQSHDVITAVANSLPSNIPIIVDPVMISTSNFKLMNDS
HHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHCCCCCCEEECCEEEECCCEEEECCC
SVFHFIEHILPKATVITPNILEAEILTNTKIIDEYCMKQASKILKSLGTKSVLIKGGHLN
HHHHHHHHHCCCCEEECCCHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCC
RETIDNIFLTEDDQIFNFSHHRIPHNELHGTGCTLSSAIASFVSQKIPISDSIDLAIQYL
HHHHCCEEECCCCCEECCCCCCCCCHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHH
LNTIKTIPKVGKGHNPVFHNYNIISN
HHHHHHHHCCCCCCCCCEECCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA