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The map label for this gene is pheS

Identifier: 58616855

GI number: 58616855

Start: 215837

End: 216871

Strand: Reverse

Name: pheS

Synonym: ERGA_CDS_01280

Alternate gene names: 58616855

Gene position: 216871-215837 (Counterclockwise)

Preceding gene: 58616856

Following gene: 58616854

Centisome position: 14.46

GC content: 29.47

Gene sequence:

>1035_bases
GTGTTGGATAATATACATAACTTACATAAAGAAGCTACCGATAAGATATTGTCTACTTCTTCTCTTGAGGAGTTAGAAAA
TATCAGGTCAGCTTACTTTGGAAAATCAGGTTTTATAACAGTATATTTAAGGAATATTTCAACAATCAAAAACCTAGAAG
AGAGAAAATCTGTAGGAAATGTTGTTAATACGATTTACTCTGAGCTAAAATCCTTAATTAATTCACATAGAACAAAATTA
CATCAGATACAGATCGATAACCAATTACTTCAAGATAAAGTTGATATTAGCCTACCAATTCGTCCTCAAAAAATAGGAAA
GTTACATCCTATTTCGAATGTTTTAAATGAGGTAAAACGTATATTCCTTTCTTTAGGATTTAAACTATATGATGGACCAG
AACTAGAAGATGAATTCCATGTTTTTGATGCACTCAATACCAATAAAAACCATCCAGCAAGAGAAGAAAATGATACTTTC
TATTTAAAAACATTAGTGAATAACAAAAGGGTTGTACTACGTACACATACATCTTCTGTACAAATTAGGACAATGGAAAA
TAATGATGGAATTTATCCAATTAAGGTTATTGCTCCAGGAAAAGTTTATAGAAATGATTGGGATGCAACACACAGTCCTA
TGTTTCATCAAATTGAAGGACTATATATTGATAAAAATATTAATATGGGGCATTTAAAATATTGTATTAATTATTTCCTG
AATAAATTCTTTGGAAAAAATGTTCAAATAAGATTCCGAAACAGTTATTTCCCCTTTACTGAACCATCAGCTGAAGTTGA
CATAAAATGCAATCAGAATGATTGGATAGAAATTTTAGGTTGTGGTATGGTTCATAGTAATGTCTTGACAAACGTTAATA
TTGACCCAAATCAATATAGTGGTTTTGCTTTTGGGATTGGTATAGAAAGAATTACTATGTTAAAATATAATATTAGTGAC
TTACGTAAGTTTTACACTAATAATCTGCAATGGTTAGATCATTACGGGTTTCACTTCACTCATTTAACTATATAA

Upstream 100 bases:

>100_bases
ATAACGCTAAATAGAAAAATTTTAGCAGATATGGCAGTGCATAATAAAGATAACTTTAAGCAGATTGTAGATTTAACTAA
GGAAGCTTTAACTAAGTCTC

Downstream 100 bases:

>100_bases
CATGTTCACTCAACCTAATAAAAATGGAGCATTAATCACAGGTAGTGCACGAAGGCTAGGTAGAGCTATGGCGATATTTC
TAGCCACAAAAGGATATGAT

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MLDNIHNLHKEATDKILSTSSLEELENIRSAYFGKSGFITVYLRNISTIKNLEERKSVGNVVNTIYSELKSLINSHRTKL
HQIQIDNQLLQDKVDISLPIRPQKIGKLHPISNVLNEVKRIFLSLGFKLYDGPELEDEFHVFDALNTNKNHPAREENDTF
YLKTLVNNKRVVLRTHTSSVQIRTMENNDGIYPIKVIAPGKVYRNDWDATHSPMFHQIEGLYIDKNINMGHLKYCINYFL
NKFFGKNVQIRFRNSYFPFTEPSAEVDIKCNQNDWIEILGCGMVHSNVLTNVNIDPNQYSGFAFGIGIERITMLKYNISD
LRKFYTNNLQWLDHYGFHFTHLTI

Sequences:

>Translated_344_residues
MLDNIHNLHKEATDKILSTSSLEELENIRSAYFGKSGFITVYLRNISTIKNLEERKSVGNVVNTIYSELKSLINSHRTKL
HQIQIDNQLLQDKVDISLPIRPQKIGKLHPISNVLNEVKRIFLSLGFKLYDGPELEDEFHVFDALNTNKNHPAREENDTF
YLKTLVNNKRVVLRTHTSSVQIRTMENNDGIYPIKVIAPGKVYRNDWDATHSPMFHQIEGLYIDKNINMGHLKYCINYFL
NKFFGKNVQIRFRNSYFPFTEPSAEVDIKCNQNDWIEILGCGMVHSNVLTNVNIDPNQYSGFAFGIGIERITMLKYNISD
LRKFYTNNLQWLDHYGFHFTHLTI
>Mature_344_residues
MLDNIHNLHKEATDKILSTSSLEELENIRSAYFGKSGFITVYLRNISTIKNLEERKSVGNVVNTIYSELKSLINSHRTKL
HQIQIDNQLLQDKVDISLPIRPQKIGKLHPISNVLNEVKRIFLSLGFKLYDGPELEDEFHVFDALNTNKNHPAREENDTF
YLKTLVNNKRVVLRTHTSSVQIRTMENNDGIYPIKVIAPGKVYRNDWDATHSPMFHQIEGLYIDKNINMGHLKYCINYFL
NKFFGKNVQIRFRNSYFPFTEPSAEVDIKCNQNDWIEILGCGMVHSNVLTNVNIDPNQYSGFAFGIGIERITMLKYNISD
LRKFYTNNLQWLDHYGFHFTHLTI

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4758340, Length=336, Percent_Identity=28.5714285714286, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI5729820, Length=230, Percent_Identity=28.695652173913, Blast_Score=106, Evalue=3e-23,
Organism=Escherichia coli, GI1788007, Length=336, Percent_Identity=45.5357142857143, Blast_Score=326, Evalue=1e-90,
Organism=Caenorhabditis elegans, GI17508957, Length=272, Percent_Identity=30.1470588235294, Blast_Score=124, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI32563657, Length=272, Percent_Identity=30.1470588235294, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI32566635, Length=246, Percent_Identity=30.8943089430894, Blast_Score=114, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6321087, Length=260, Percent_Identity=30.3846153846154, Blast_Score=120, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6325304, Length=265, Percent_Identity=26.0377358490566, Blast_Score=107, Evalue=2e-24,
Organism=Drosophila melanogaster, GI18858079, Length=264, Percent_Identity=30.3030303030303, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI17137424, Length=244, Percent_Identity=26.6393442622951, Blast_Score=102, Evalue=5e-22,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_EHRRG (Q5FH45)

Other databases:

- EMBL:   CR925677
- RefSeq:   YP_196054.1
- ProteinModelPortal:   Q5FH45
- SMR:   Q5FH45
- STRING:   Q5FH45
- GeneID:   3267823
- GenomeReviews:   CR925677_GR
- KEGG:   erg:ERGA_CDS_01280
- eggNOG:   COG0016
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- ProtClustDB:   PRK00488
- BioCyc:   ERUM302409:ERGA_CDS_01280-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 40030; Mature: 40030

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDNIHNLHKEATDKILSTSSLEELENIRSAYFGKSGFITVYLRNISTIKNLEERKSVGN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHHHHHHHH
VVNTIYSELKSLINSHRTKLHQIQIDNQLLQDKVDISLPIRPQKIGKLHPISNVLNEVKR
HHHHHHHHHHHHHHHHHHEEEEEEECHHHHHCCCCEECCCCHHHCCCCCHHHHHHHHHHH
IFLSLGFKLYDGPELEDEFHVFDALNTNKNHPAREENDTFYLKTLVNNKRVVLRTHTSSV
HHHHCCCEEECCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEEECCCEEEEEEECCEE
QIRTMENNDGIYPIKVIAPGKVYRNDWDATHSPMFHQIEGLYIDKNINMGHLKYCINYFL
EEEEEECCCCEEEEEEEECCCEECCCCCCCCCCHHHHCCCEEEECCCCHHHHHHHHHHHH
NKFFGKNVQIRFRNSYFPFTEPSAEVDIKCNQNDWIEILGCGMVHSNVLTNVNIDPNQYS
HHHHCCCEEEEEECCCCCCCCCCCCEEEEECCCCCEEEEECCHHHCCCEEEECCCCCCCC
GFAFGIGIERITMLKYNISDLRKFYTNNLQWLDHYGFHFTHLTI
CEEEEECCEEEEEEEECHHHHHHHHCCCCCEECCCCEEEEEEEC
>Mature Secondary Structure
MLDNIHNLHKEATDKILSTSSLEELENIRSAYFGKSGFITVYLRNISTIKNLEERKSVGN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHHHHHHHH
VVNTIYSELKSLINSHRTKLHQIQIDNQLLQDKVDISLPIRPQKIGKLHPISNVLNEVKR
HHHHHHHHHHHHHHHHHHEEEEEEECHHHHHCCCCEECCCCHHHCCCCCHHHHHHHHHHH
IFLSLGFKLYDGPELEDEFHVFDALNTNKNHPAREENDTFYLKTLVNNKRVVLRTHTSSV
HHHHCCCEEECCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEEECCCEEEEEEECCEE
QIRTMENNDGIYPIKVIAPGKVYRNDWDATHSPMFHQIEGLYIDKNINMGHLKYCINYFL
EEEEEECCCCEEEEEEEECCCEECCCCCCCCCCHHHHCCCEEEECCCCHHHHHHHHHHHH
NKFFGKNVQIRFRNSYFPFTEPSAEVDIKCNQNDWIEILGCGMVHSNVLTNVNIDPNQYS
HHHHCCCEEEEEECCCCCCCCCCCCEEEEECCCCCEEEEECCHHHCCCEEEECCCCCCCC
GFAFGIGIERITMLKYNISDLRKFYTNNLQWLDHYGFHFTHLTI
CEEEEECCEEEEEEEECHHHHHHHHCCCCCEECCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA