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The map label for this gene is ispF [H]

Identifier: 58616822

GI number: 58616822

Start: 157895

End: 158449

Strand: Reverse

Name: ispF [H]

Synonym: ERGA_CDS_00950

Alternate gene names: 58616822

Gene position: 158449-157895 (Counterclockwise)

Preceding gene: 58616823

Following gene: 58616821

Centisome position: 10.56

GC content: 31.89

Gene sequence:

>555_bases
GTGTTGCCAATGTCACCAATATATAAATGCTTTATGAGTAAACACACAAACATACCAATGTTTAGAATTGGAATAGGATA
TGATGTACATAGATTTGATAATATAAATAATGACGATAGTAATACATCTATCACAATATGTGGAATAGAAATTAATTATC
ACAAAAAAATTATAGCGCATTCAGATGGAGATGTAGGATTACATGCTCTAGCAGATGCAATACTAGGGGCAGTAGGATGT
GGAAGCATAGGACAACACTTTCCTAATACAGACCAAAAGTGGAAAAATGCAAAATCTAGTCATTTTGTAATAGAGGCACA
AAAGAAAGCACAAGAAAGGGGATATATCATTTCAAATGCAGATATTATAATTATATGCGAACAACCAAAAATAATGCCTT
ATGCACTAGAAATGCAACATTATATTGCTCAATTTACAAGTATAGATCCATCATTTATTAACATTAAAGCTACAACCACA
GAAAAACTAGGAGCCATTGGCAGAAATGAAGGAATAGCTGCACAAGCAATAGTTTTATGCAGTCAACAATATTAA

Upstream 100 bases:

>100_bases
ACATTACTTAATTAAAATGTATAGAGACAGATAACTTTAAGGTATATAAACAAAATGTTTTCTATTTATTTAAAATATCA
TATGATAAAAACTTATATAA

Downstream 100 bases:

>100_bases
TGTTACATCCTTCAATAATTTACTCACTAATGAACCTAACACAAGGTACTAAAAAACTATATTAATAATTAACTGACTGA
AGACTTGAACAATAGACCTT

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS [H]

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MLPMSPIYKCFMSKHTNIPMFRIGIGYDVHRFDNINNDDSNTSITICGIEINYHKKIIAHSDGDVGLHALADAILGAVGC
GSIGQHFPNTDQKWKNAKSSHFVIEAQKKAQERGYIISNADIIIICEQPKIMPYALEMQHYIAQFTSIDPSFINIKATTT
EKLGAIGRNEGIAAQAIVLCSQQY

Sequences:

>Translated_184_residues
MLPMSPIYKCFMSKHTNIPMFRIGIGYDVHRFDNINNDDSNTSITICGIEINYHKKIIAHSDGDVGLHALADAILGAVGC
GSIGQHFPNTDQKWKNAKSSHFVIEAQKKAQERGYIISNADIIIICEQPKIMPYALEMQHYIAQFTSIDPSFINIKATTT
EKLGAIGRNEGIAAQAIVLCSQQY
>Mature_184_residues
MLPMSPIYKCFMSKHTNIPMFRIGIGYDVHRFDNINNDDSNTSITICGIEINYHKKIIAHSDGDVGLHALADAILGAVGC
GSIGQHFPNTDQKWKNAKSSHFVIEAQKKAQERGYIISNADIIIICEQPKIMPYALEMQHYIAQFTSIDPSFINIKATTT
EKLGAIGRNEGIAAQAIVLCSQQY

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP [H]

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family [H]

Homologues:

Organism=Escherichia coli, GI1789103, Length=159, Percent_Identity=45.9119496855346, Blast_Score=139, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003526
- InterPro:   IPR020555 [H]

Pfam domain/function: PF02542 YgbB [H]

EC number: =4.6.1.12 [H]

Molecular weight: Translated: 20400; Mature: 20400

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPMSPIYKCFMSKHTNIPMFRIGIGYDVHRFDNINNDDSNTSITICGIEINYHKKIIAH
CCCCHHHHHHHHHCCCCCCEEEEECCCEEHHCCCCCCCCCCCEEEEEEEEEECCEEEEEE
SDGDVGLHALADAILGAVGCGSIGQHFPNTDQKWKNAKSSHFVIEAQKKAQERGYIISNA
CCCCCHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHCCCCCEEEEEEHHHHHHCCEEEECC
DIIIICEQPKIMPYALEMQHYIAQFTSIDPSFINIKATTTEKLGAIGRNEGIAAQAIVLC
CEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHCCCCCCCCCEEEEEEE
SQQY
CCCC
>Mature Secondary Structure
MLPMSPIYKCFMSKHTNIPMFRIGIGYDVHRFDNINNDDSNTSITICGIEINYHKKIIAH
CCCCHHHHHHHHHCCCCCCEEEEECCCEEHHCCCCCCCCCCCEEEEEEEEEECCEEEEEE
SDGDVGLHALADAILGAVGCGSIGQHFPNTDQKWKNAKSSHFVIEAQKKAQERGYIISNA
CCCCCHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHCCCCCEEEEEEHHHHHHCCEEEECC
DIIIICEQPKIMPYALEMQHYIAQFTSIDPSFINIKATTTEKLGAIGRNEGIAAQAIVLC
CEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHCCCCCCCCCEEEEEEE
SQQY
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA