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The map label for this gene is tuf [H]

Identifier: 161986607

GI number: 161986607

Start: 994549

End: 995736

Strand: Reverse

Name: tuf [H]

Synonym: ERGA_CDS_06310

Alternate gene names: 161986607

Gene position: 995736-994549 (Counterclockwise)

Preceding gene: 58617368

Following gene: 58617357

Centisome position: 66.39

GC content: 38.97

Gene sequence:

>1188_bases
ATGGTAGATGGAAGGAAGCCGCATATAAATGTTGGAACGATAGGACATGTTGATCATGGTAAGACAACATTAACTGCTGC
ATTAACGACAGTATTAGCGAAGAGATTAAGTGGTGAAGGGAATAAGAGTGTCAAGTATGATGAGATAGATAAAGCACCGG
AAGAGAAAGCGAGAGGGATTACCATTTCAACAGCACATGTAGAGTATGAAACAGAGAATAGGCATTATGCGCATGTAGAT
TGTCCGGGGCATGCTGATTATATAAAGAATATGATCACAGGGGCAGCTCAAATGGATGCTGCGATATTAGTAGTATCTGC
TACTGATGGTGCGATGCCGCAGACAAGGGAGCATATTTTGCTAGCGAAGCAGGTAGGGGTAAAGGACATAGTAGTATGGA
TGAATAAGTGTGATGTAGTAGATGATGAAGAGATGCTATCGTTAGTTGAGATGGAGATAAGAGAGTTATTAACAAAGTAT
GGATATCCTGGTGATGATATAGATGTAGTGAAAGGATCTGCAGTTAAGGCATTAGAAGAAGAATCAGCTGATGGTGTCTG
GAGCGAGAAGATAATGGAATTGATGAATGCTTTAGAGAAGATAGATTTACCAATAAGAGAGAAAGACAAGCCATTTTTGA
TGTCTATAGAGGATGTATTTTCAATACCCGGGAGAGGGACAGTAGTAACAGGAAGGATAGAGAGAGGAGTAATAAAGGTA
GGGGACAAGATAGATATAGTAGGATTAAGAGATATACAAAGTACAGTATGTACAGGTGTAGAAATGTTTCATAAGGCTTT
GGATGCTGGTGAGGCAGGAGATAATGCTGGGATATTGTTAAGAGGTATAAAGAAAGAGGATGTAGAGAGGGGACAGGTAT
TAAGTGCACCTGGACAGATACATTCATATAAGGGGTTCAAGGCAGAGGTATATGTATTAAAGAAAGAGGAGGGTGGAAGG
CACACTCCATTTTTTTCAAATTATCAGCCACAATTTTATGTTAGGACAACTGATGTAACAGGTAATATAAAATTACCTGA
TGGGGTAGAGATGGTAATGCCTGGAGATAATATAAGTATCGAAGTGAATTTAGATAAGCCAGTTGCGATTGATAAAGGGT
TAAGATTTGCAATTCGTGAAGGTGGTAGAACTATTGGTTCTGGTATTATTACTGAAATTTTGGAGTAA

Upstream 100 bases:

>100_bases
AATGGTAGAGTGCCAGCCTTCCAAGCTGGTTATGTGGGTTCGATTCCCACTATCCGCTCTTTATTTGGGATGGTTTTATG
GTGTTAAAGGTATATTGTTT

Downstream 100 bases:

>100_bases
GTGGTTATGGTAACGCAAAAGATATATATTGAGTTAAAGGCCTTTGATAGCTATTTATTAGATAGGTCTGCCCGTAGCAT
TATTTTGACTGCTAAAAGGA

Product: elongation factor Tu

Products: NA

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MVDGRKPHINVGTIGHVDHGKTTLTAALTTVLAKRLSGEGNKSVKYDEIDKAPEEKARGITISTAHVEYETENRHYAHVD
CPGHADYIKNMITGAAQMDAAILVVSATDGAMPQTREHILLAKQVGVKDIVVWMNKCDVVDDEEMLSLVEMEIRELLTKY
GYPGDDIDVVKGSAVKALEEESADGVWSEKIMELMNALEKIDLPIREKDKPFLMSIEDVFSIPGRGTVVTGRIERGVIKV
GDKIDIVGLRDIQSTVCTGVEMFHKALDAGEAGDNAGILLRGIKKEDVERGQVLSAPGQIHSYKGFKAEVYVLKKEEGGR
HTPFFSNYQPQFYVRTTDVTGNIKLPDGVEMVMPGDNISIEVNLDKPVAIDKGLRFAIREGGRTIGSGIITEILE

Sequences:

>Translated_395_residues
MVDGRKPHINVGTIGHVDHGKTTLTAALTTVLAKRLSGEGNKSVKYDEIDKAPEEKARGITISTAHVEYETENRHYAHVD
CPGHADYIKNMITGAAQMDAAILVVSATDGAMPQTREHILLAKQVGVKDIVVWMNKCDVVDDEEMLSLVEMEIRELLTKY
GYPGDDIDVVKGSAVKALEEESADGVWSEKIMELMNALEKIDLPIREKDKPFLMSIEDVFSIPGRGTVVTGRIERGVIKV
GDKIDIVGLRDIQSTVCTGVEMFHKALDAGEAGDNAGILLRGIKKEDVERGQVLSAPGQIHSYKGFKAEVYVLKKEEGGR
HTPFFSNYQPQFYVRTTDVTGNIKLPDGVEMVMPGDNISIEVNLDKPVAIDKGLRFAIREGGRTIGSGIITEILE
>Mature_395_residues
MVDGRKPHINVGTIGHVDHGKTTLTAALTTVLAKRLSGEGNKSVKYDEIDKAPEEKARGITISTAHVEYETENRHYAHVD
CPGHADYIKNMITGAAQMDAAILVVSATDGAMPQTREHILLAKQVGVKDIVVWMNKCDVVDDEEMLSLVEMEIRELLTKY
GYPGDDIDVVKGSAVKALEEESADGVWSEKIMELMNALEKIDLPIREKDKPFLMSIEDVFSIPGRGTVVTGRIERGVIKV
GDKIDIVGLRDIQSTVCTGVEMFHKALDAGEAGDNAGILLRGIKKEDVERGQVLSAPGQIHSYKGFKAEVYVLKKEEGGR
HTPFFSNYQPQFYVRTTDVTGNIKLPDGVEMVMPGDNISIEVNLDKPVAIDKGLRFAIREGGRTIGSGIITEILE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=390, Percent_Identity=54.6153846153846, Blast_Score=446, Evalue=1e-125,
Organism=Homo sapiens, GI4503471, Length=439, Percent_Identity=31.8906605922551, Blast_Score=172, Evalue=5e-43,
Organism=Homo sapiens, GI223555963, Length=434, Percent_Identity=29.4930875576037, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI5729864, Length=434, Percent_Identity=29.4930875576037, Blast_Score=166, Evalue=5e-41,
Organism=Homo sapiens, GI4503475, Length=448, Percent_Identity=30.3571428571429, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI54607086, Length=329, Percent_Identity=29.483282674772, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI194097354, Length=336, Percent_Identity=31.25, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI194018522, Length=336, Percent_Identity=31.25, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI194018520, Length=336, Percent_Identity=31.25, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI46094014, Length=336, Percent_Identity=30.6547619047619, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI4503507, Length=283, Percent_Identity=28.2685512367491, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI94966754, Length=136, Percent_Identity=33.0882352941176, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI157426893, Length=240, Percent_Identity=29.1666666666667, Blast_Score=69, Evalue=8e-12,
Organism=Escherichia coli, GI1790412, Length=390, Percent_Identity=67.6923076923077, Blast_Score=529, Evalue=1e-151,
Organism=Escherichia coli, GI1789737, Length=390, Percent_Identity=67.6923076923077, Blast_Score=528, Evalue=1e-151,
Organism=Escherichia coli, GI2367247, Length=377, Percent_Identity=28.3819628647215, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1789108, Length=254, Percent_Identity=31.8897637795276, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI48994988, Length=289, Percent_Identity=29.4117647058824, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17556456, Length=396, Percent_Identity=51.7676767676768, Blast_Score=386, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI25141371, Length=390, Percent_Identity=44.6153846153846, Blast_Score=328, Evalue=3e-90,
Organism=Caenorhabditis elegans, GI17552884, Length=439, Percent_Identity=30.0683371298405, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17569207, Length=439, Percent_Identity=30.0683371298405, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI32566303, Length=418, Percent_Identity=28.9473684210526, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI115532067, Length=435, Percent_Identity=27.816091954023, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI115532065, Length=435, Percent_Identity=27.5862068965517, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI32566629, Length=346, Percent_Identity=29.7687861271676, Blast_Score=124, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI17506081, Length=249, Percent_Identity=31.3253012048193, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI32566301, Length=145, Percent_Identity=42.7586206896552, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17557151, Length=235, Percent_Identity=25.531914893617, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6324761, Length=399, Percent_Identity=58.6466165413534, Blast_Score=474, Evalue=1e-135,
Organism=Saccharomyces cerevisiae, GI6325337, Length=439, Percent_Identity=31.4350797266515, Blast_Score=167, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6319594, Length=439, Percent_Identity=31.4350797266515, Blast_Score=167, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6322937, Length=449, Percent_Identity=27.8396436525612, Blast_Score=128, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6320377, Length=356, Percent_Identity=29.4943820224719, Blast_Score=93, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6320863, Length=277, Percent_Identity=27.4368231046931, Blast_Score=86, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6323320, Length=241, Percent_Identity=28.2157676348548, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI281363316, Length=390, Percent_Identity=57.6923076923077, Blast_Score=460, Evalue=1e-130,
Organism=Drosophila melanogaster, GI17864358, Length=390, Percent_Identity=57.6923076923077, Blast_Score=460, Evalue=1e-130,
Organism=Drosophila melanogaster, GI19921738, Length=390, Percent_Identity=47.9487179487179, Blast_Score=365, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24652838, Length=447, Percent_Identity=30.4250559284116, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI17137572, Length=447, Percent_Identity=30.4250559284116, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI45553807, Length=446, Percent_Identity=30.0448430493274, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI45553816, Length=446, Percent_Identity=30.0448430493274, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI24651721, Length=446, Percent_Identity=30.0448430493274, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI17864154, Length=446, Percent_Identity=30.0448430493274, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI45550900, Length=435, Percent_Identity=28.735632183908, Blast_Score=141, Evalue=8e-34,
Organism=Drosophila melanogaster, GI17137380, Length=342, Percent_Identity=31.2865497076023, Blast_Score=131, Evalue=9e-31,
Organism=Drosophila melanogaster, GI19922690, Length=310, Percent_Identity=30.3225806451613, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24647048, Length=369, Percent_Identity=27.10027100271, Blast_Score=88, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24647046, Length=369, Percent_Identity=27.10027100271, Blast_Score=88, Evalue=9e-18,
Organism=Drosophila melanogaster, GI28572034, Length=177, Percent_Identity=33.8983050847458, Blast_Score=65, Evalue=9e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: NA

Molecular weight: Translated: 43282; Mature: 43282

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDGRKPHINVGTIGHVDHGKTTLTAALTTVLAKRLSGEGNKSVKYDEIDKAPEEKARGI
CCCCCCCEEEECEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCHHHHHCCE
TISTAHVEYETENRHYAHVDCPGHADYIKNMITGAAQMDAAILVVSATDGAMPQTREHIL
EEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCEEEEEEEECCCCCCCCHHHEE
LAKQVGVKDIVVWMNKCDVVDDEEMLSLVEMEIRELLTKYGYPGDDIDVVKGSAVKALEE
EHHHCCHHEEEEEHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHH
ESADGVWSEKIMELMNALEKIDLPIREKDKPFLMSIEDVFSIPGRGTVVTGRIERGVIKV
HCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHCCCCCCEEEEEEECCCEEEE
GDKIDIVGLRDIQSTVCTGVEMFHKALDAGEAGDNAGILLRGIKKEDVERGQVLSAPGQI
CCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHCCCCEECCCCCC
HSYKGFKAEVYVLKKEEGGRHTPFFSNYQPQFYVRTTDVTGNIKLPDGVEMVMPGDNISI
CCCCCCEEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCEEECCCCCEEEECCCCEEE
EVNLDKPVAIDKGLRFAIREGGRTIGSGIITEILE
EEECCCCCCCCCCCEEEEHHCCCHHHHHHHHHHCC
>Mature Secondary Structure
MVDGRKPHINVGTIGHVDHGKTTLTAALTTVLAKRLSGEGNKSVKYDEIDKAPEEKARGI
CCCCCCCEEEECEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCHHHHHCCE
TISTAHVEYETENRHYAHVDCPGHADYIKNMITGAAQMDAAILVVSATDGAMPQTREHIL
EEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCEEEEEEEECCCCCCCCHHHEE
LAKQVGVKDIVVWMNKCDVVDDEEMLSLVEMEIRELLTKYGYPGDDIDVVKGSAVKALEE
EHHHCCHHEEEEEHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHH
ESADGVWSEKIMELMNALEKIDLPIREKDKPFLMSIEDVFSIPGRGTVVTGRIERGVIKV
HCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHCCCCCCEEEEEEECCCEEEE
GDKIDIVGLRDIQSTVCTGVEMFHKALDAGEAGDNAGILLRGIKKEDVERGQVLSAPGQI
CCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHCCCCEECCCCCC
HSYKGFKAEVYVLKKEEGGRHTPFFSNYQPQFYVRTTDVTGNIKLPDGVEMVMPGDNISI
CCCCCCEEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCEEECCCCCEEEECCCCEEE
EVNLDKPVAIDKGLRFAIREGGRTIGSGIITEILE
EEECCCCCCCCCCCEEEEHHCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA