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The map label for this gene is nplT [H]

Identifier: 56965789

GI number: 56965789

Start: 4199400

End: 4201169

Strand: Direct

Name: nplT [H]

Synonym: ABC4031

Alternate gene names: 56965789

Gene position: 4199400-4201169 (Clockwise)

Preceding gene: 56965774

Following gene: 56965792

Centisome position: 97.57

GC content: 44.12

Gene sequence:

>1770_bases
ATGAAAGTAGCGATCTACCATCGCCCGAAAAGTGAAGATGCCTACGCGTATGACAAAGATACGATCCACATTCGTCTGCG
CACGCGCAAAGACGATGCTCACGAGGTCATGCTTATTTATGGAGATCCTTACGATTGGGCGGGAAACGAATGGGTGACTG
CCAAAAAAACAATGACACAAGCAGGGGCCGATGGAGAATATGATTATTGGTTTGTGGAAGTGAAGCCGCCCTACCATCGC
CTTCGTTACGGGTTCGAAATTAAGGCAAAAGATGAGACGATTTACTATGGAGAAAGTGGGTTTTGCGATACGCCTGCAAA
AGACATTAGCGATTATTTCTGTTTTCCGTTTATGAATCAAGCCGATTTGTTTACACCGCCTGAATGGGTTAAAGAAACGA
TCTGGTATCAAATCTTTCCTGAACGTTTTGCCAATGGCGATCATTCCCGTGACCCTGAAAATACATTGGCTTGGGGAAGT
GCACCACCGACTGCAACCAATTATTTCGGCGGTGACCTTCAAGGGATTGTCGATCATCTTGATTATTTGCAGAAGCTAGG
AATTAACGGCATTTATTTAACACCAATCTTTAAAGCGTTTTCCAACCATAAATACGATACGATTGATTATTTAAAAGTAG
ATCCCCAATTTGGAGATGAAACGACACTTAAACTGCTTGTCGATGAATGCCATAAGCGCGGCATTCGTGTCATGCTTGAT
GCCGTCTTCAACCATGCAGGCCTGTATTTTCCACCTTTTCAAGATGTACTAAAACACCAGCAGGAATCAGAATATAGGGA
TTGGTTTCATATCCGCCAATTTCCTGTTCGAGCAGAGGAACCACCTAATTACGATACGTTCGCCTTCACGCCTTTAATGC
CGAAACTAAATACAGCAAATGAAGCAGTAAAGGACTATTTATTGAACGTAGCTACGTATTGGATTAAGGAGTTTGACATT
GACGGTTGGCGTTTAGATGTCGCCAATGAAGTCGACCATGCCTTCTGGCGTGAGTTTCGCAACAGAGTCAAAGCATTAAA
ACCAGATTTGTATATTCTCGGTGAAATATGGCATAACGCTTACCCTTGGCTGCAAGGCGACCAATTCGATGGGGTCATGA
ACTACCCGCTCACTGACGCGATTTTTGACTTTGTCCAACACGGCAAAAGCGCAACCACTTTTCAATACGCGGTCACAAAA
CATTTGTACAGTTACCCAAAATTGGTGAACGAAGTCCAGTTCAATTTGCTCGGCAGCCATGACACACCAAGAATTTTAAC
TGTAAGTGGGGGCAACAAAAAATTAGTCAAACTTTCCTACTTATTGCTGTTTTCCTTTCCTGGCTCACCTTGTATTTATT
ACGGGGATGAAATCGGCATGGCCGGAGAAAATGACCCTGGTTGCCGCGCGTGCATGGTGTGGGAGGAAGACCAACAAGAT
CAGGAGCTATTCCAATTTCTGCAAAAACTAATTAAGATCCGCAAAACTCACCAAGCAATGGGCACCTATAACACCTTAAA
ATTTTATGACTCTGGCACTGAGGATGTGCTGTTGTATTCCACATCCGCTGGCGGAGAGCTTCTTTTATTTGCGGTTAACC
GCAGTAAACAAGAAGCAACGGTCAAACTCCCTGATTCACTAAAGAGCCTTGCATTTGTCGATGTCATTTGCGAAAGCCCA
TTGCAAAGCGAAGAGCTCCACGTTGAAGGCGAGTGCTTTTTGTTCCTTCATTCTGTCGACGCCAACAAATGCGGCCAAGT
AGACAAGTAA

Upstream 100 bases:

>100_bases
GTTTGCCTTTTTTCTTGACTTTCACTCTTTCATCTCATAGGCTGAAAATAACAATCAAGAAAACGCTTACGATTCTCGTT
AAGAAGGGAGCTTGTTTTGA

Downstream 100 bases:

>100_bases
AAAAACAGAATGGCATAGCTCATGACGAGTTATGCCATTCGTGCCGTAGTCTCCCTTCCTACATATAAAAAAGCCTGTCG
ACCATCTTAACACTTGTCAA

Product: maltogenic amylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 589; Mature: 589

Protein sequence:

>589_residues
MKVAIYHRPKSEDAYAYDKDTIHIRLRTRKDDAHEVMLIYGDPYDWAGNEWVTAKKTMTQAGADGEYDYWFVEVKPPYHR
LRYGFEIKAKDETIYYGESGFCDTPAKDISDYFCFPFMNQADLFTPPEWVKETIWYQIFPERFANGDHSRDPENTLAWGS
APPTATNYFGGDLQGIVDHLDYLQKLGINGIYLTPIFKAFSNHKYDTIDYLKVDPQFGDETTLKLLVDECHKRGIRVMLD
AVFNHAGLYFPPFQDVLKHQQESEYRDWFHIRQFPVRAEEPPNYDTFAFTPLMPKLNTANEAVKDYLLNVATYWIKEFDI
DGWRLDVANEVDHAFWREFRNRVKALKPDLYILGEIWHNAYPWLQGDQFDGVMNYPLTDAIFDFVQHGKSATTFQYAVTK
HLYSYPKLVNEVQFNLLGSHDTPRILTVSGGNKKLVKLSYLLLFSFPGSPCIYYGDEIGMAGENDPGCRACMVWEEDQQD
QELFQFLQKLIKIRKTHQAMGTYNTLKFYDSGTEDVLLYSTSAGGELLLFAVNRSKQEATVKLPDSLKSLAFVDVICESP
LQSEELHVEGECFLFLHSVDANKCGQVDK

Sequences:

>Translated_589_residues
MKVAIYHRPKSEDAYAYDKDTIHIRLRTRKDDAHEVMLIYGDPYDWAGNEWVTAKKTMTQAGADGEYDYWFVEVKPPYHR
LRYGFEIKAKDETIYYGESGFCDTPAKDISDYFCFPFMNQADLFTPPEWVKETIWYQIFPERFANGDHSRDPENTLAWGS
APPTATNYFGGDLQGIVDHLDYLQKLGINGIYLTPIFKAFSNHKYDTIDYLKVDPQFGDETTLKLLVDECHKRGIRVMLD
AVFNHAGLYFPPFQDVLKHQQESEYRDWFHIRQFPVRAEEPPNYDTFAFTPLMPKLNTANEAVKDYLLNVATYWIKEFDI
DGWRLDVANEVDHAFWREFRNRVKALKPDLYILGEIWHNAYPWLQGDQFDGVMNYPLTDAIFDFVQHGKSATTFQYAVTK
HLYSYPKLVNEVQFNLLGSHDTPRILTVSGGNKKLVKLSYLLLFSFPGSPCIYYGDEIGMAGENDPGCRACMVWEEDQQD
QELFQFLQKLIKIRKTHQAMGTYNTLKFYDSGTEDVLLYSTSAGGELLLFAVNRSKQEATVKLPDSLKSLAFVDVICESP
LQSEELHVEGECFLFLHSVDANKCGQVDK
>Mature_589_residues
MKVAIYHRPKSEDAYAYDKDTIHIRLRTRKDDAHEVMLIYGDPYDWAGNEWVTAKKTMTQAGADGEYDYWFVEVKPPYHR
LRYGFEIKAKDETIYYGESGFCDTPAKDISDYFCFPFMNQADLFTPPEWVKETIWYQIFPERFANGDHSRDPENTLAWGS
APPTATNYFGGDLQGIVDHLDYLQKLGINGIYLTPIFKAFSNHKYDTIDYLKVDPQFGDETTLKLLVDECHKRGIRVMLD
AVFNHAGLYFPPFQDVLKHQQESEYRDWFHIRQFPVRAEEPPNYDTFAFTPLMPKLNTANEAVKDYLLNVATYWIKEFDI
DGWRLDVANEVDHAFWREFRNRVKALKPDLYILGEIWHNAYPWLQGDQFDGVMNYPLTDAIFDFVQHGKSATTFQYAVTK
HLYSYPKLVNEVQFNLLGSHDTPRILTVSGGNKKLVKLSYLLLFSFPGSPCIYYGDEIGMAGENDPGCRACMVWEEDQQD
QELFQFLQKLIKIRKTHQAMGTYNTLKFYDSGTEDVLLYSTSAGGELLLFAVNRSKQEATVKLPDSLKSLAFVDVICESP
LQSEELHVEGECFLFLHSVDANKCGQVDK

Specific function: Hydrolyzes pullulan efficiently but only a small amount of starch. Endohydrolysis of 1,4-alpha-glucosidic linkages in pullulan to form panose. Cleaves also (1-6)-alpha-glucosidic linkages to form maltotriose [H]

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1786604, Length=515, Percent_Identity=32.621359223301, Blast_Score=234, Evalue=1e-62,
Organism=Escherichia coli, GI1789995, Length=495, Percent_Identity=25.0505050505051, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1790687, Length=390, Percent_Identity=27.1794871794872, Blast_Score=114, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6322245, Length=229, Percent_Identity=27.9475982532751, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6321726, Length=460, Percent_Identity=23.4782608695652, Blast_Score=80, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6322241, Length=232, Percent_Identity=25.8620689655172, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6322021, Length=232, Percent_Identity=25.8620689655172, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324416, Length=232, Percent_Identity=25.8620689655172, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319776, Length=417, Percent_Identity=22.7817745803357, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6321731, Length=417, Percent_Identity=22.7817745803357, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI221330053, Length=227, Percent_Identity=27.3127753303965, Blast_Score=80, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24586593, Length=430, Percent_Identity=22.7906976744186, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24583749, Length=407, Percent_Identity=24.0786240786241, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24583747, Length=407, Percent_Identity=24.0786240786241, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24586587, Length=223, Percent_Identity=26.457399103139, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI45549022, Length=407, Percent_Identity=22.1130221130221, Blast_Score=74, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24583745, Length=225, Percent_Identity=26.6666666666667, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24586589, Length=401, Percent_Identity=22.9426433915212, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24586599, Length=227, Percent_Identity=25.1101321585903, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004185
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02903 Alpha-amylase_N [H]

EC number: =3.2.1.135 [H]

Molecular weight: Translated: 68135; Mature: 68135

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVAIYHRPKSEDAYAYDKDTIHIRLRTRKDDAHEVMLIYGDPYDWAGNEWVTAKKTMTQ
CEEEEEECCCCCCCEEECCCEEEEEEEECCCCCCEEEEEECCCCCCCCCCEEEHHHHHHH
AGADGEYDYWFVEVKPPYHRLRYGFEIKAKDETIYYGESGFCDTPAKDISDYFCFPFMNQ
CCCCCCCEEEEEEECCCHHHHHCCEEEEECCCEEEECCCCCCCCCHHHHHHEECCCCCCC
ADLFTPPEWVKETIWYQIFPERFANGDHSRDPENTLAWGSAPPTATNYFGGDLQGIVDHL
CCCCCCHHHHHHHHHHEECHHHHCCCCCCCCCCCCEECCCCCCCCCCCCCCHHHHHHHHH
DYLQKLGINGIYLTPIFKAFSNHKYDTIDYLKVDPQFGDETTLKLLVDECHKRGIRVMLD
HHHHHCCCCCEEHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHH
AVFNHAGLYFPPFQDVLKHQQESEYRDWFHIRQFPVRAEEPPNYDTFAFTPLMPKLNTAN
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCCHH
EAVKDYLLNVATYWIKEFDIDGWRLDVANEVDHAFWREFRNRVKALKPDLYILGEIWHNA
HHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHCC
YPWLQGDQFDGVMNYPLTDAIFDFVQHGKSATTFQYAVTKHLYSYPKLVNEVQFNLLGSH
CCCCCCCCCCCEECCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCC
DTPRILTVSGGNKKLVKLSYLLLFSFPGSPCIYYGDEIGMAGENDPGCRACMVWEEDQQD
CCCEEEEEECCCCEEEEEEEEEEEECCCCCEEEECCCCCCCCCCCCCCEEEEEECCCCCH
QELFQFLQKLIKIRKTHQAMGTYNTLKFYDSGTEDVLLYSTSAGGELLLFAVNRSKQEAT
HHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEECCCCEEEEEEECCCCCCEE
VKLPDSLKSLAFVDVICESPLQSEELHVEGECFLFLHSVDANKCGQVDK
EECCHHHHHHHHHHHHHCCCCCCCCEEECCEEEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MKVAIYHRPKSEDAYAYDKDTIHIRLRTRKDDAHEVMLIYGDPYDWAGNEWVTAKKTMTQ
CEEEEEECCCCCCCEEECCCEEEEEEEECCCCCCEEEEEECCCCCCCCCCEEEHHHHHHH
AGADGEYDYWFVEVKPPYHRLRYGFEIKAKDETIYYGESGFCDTPAKDISDYFCFPFMNQ
CCCCCCCEEEEEEECCCHHHHHCCEEEEECCCEEEECCCCCCCCCHHHHHHEECCCCCCC
ADLFTPPEWVKETIWYQIFPERFANGDHSRDPENTLAWGSAPPTATNYFGGDLQGIVDHL
CCCCCCHHHHHHHHHHEECHHHHCCCCCCCCCCCCEECCCCCCCCCCCCCCHHHHHHHHH
DYLQKLGINGIYLTPIFKAFSNHKYDTIDYLKVDPQFGDETTLKLLVDECHKRGIRVMLD
HHHHHCCCCCEEHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHH
AVFNHAGLYFPPFQDVLKHQQESEYRDWFHIRQFPVRAEEPPNYDTFAFTPLMPKLNTAN
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCCHH
EAVKDYLLNVATYWIKEFDIDGWRLDVANEVDHAFWREFRNRVKALKPDLYILGEIWHNA
HHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHCC
YPWLQGDQFDGVMNYPLTDAIFDFVQHGKSATTFQYAVTKHLYSYPKLVNEVQFNLLGSH
CCCCCCCCCCCEECCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCC
DTPRILTVSGGNKKLVKLSYLLLFSFPGSPCIYYGDEIGMAGENDPGCRACMVWEEDQQD
CCCEEEEEECCCCEEEEEEEEEEEECCCCCEEEECCCCCCCCCCCCCCEEEEEECCCCCH
QELFQFLQKLIKIRKTHQAMGTYNTLKFYDSGTEDVLLYSTSAGGELLLFAVNRSKQEAT
HHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEECCCCEEEEEEECCCCCCEE
VKLPDSLKSLAFVDVICESPLQSEELHVEGECFLFLHSVDANKCGQVDK
EECCHHHHHHHHHHHHHCCCCCCCCEEECCEEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2482332; 12547200 [H]