Click here to switch to the map view.

The map label for this gene is thiD [H]

Identifier: 56965690

GI number: 56965690

Start: 4088712

End: 4089524

Strand: Direct

Name: thiD [H]

Synonym: ABC3932

Alternate gene names: 56965690

Gene position: 4088712-4089524 (Clockwise)

Preceding gene: 56965681

Following gene: 56965694

Centisome position: 95.0

GC content: 48.22

Gene sequence:

>813_bases
ATGGAACGTTATAAAGCATTAACAATTGCTGGCTCAGATACAAGCGGTGGTGCCGGCATTCAAGCGGACTTGAAGACATT
TCAAGAACTTGGCGTTTATGGCATGAATGCCTTGACGACTATTGTTGCACAAAATCCGTTTGAAAACTGGGCCCATGAAG
TATTCCCCCAAGATGCCAAACTCGTCGGCAAACAAATAGACACAGTGTTAAAAGGCATTGGCGCTGATGCGGTTAAAACA
GGAATGCTTGGATCGATTGAAATCATTGAACTTGTTGCCAACAAAATTGAAGAGTATGGCGTTAACAAGGTTGTTGTCGA
TCCAGTGATGGTTTGCAAAGGCGCAGATGAAGCGCTCAATCCAGAAACGGATGCATGCCTGCGCGATGTCCTCGTACCAA
AAGCATTTGTCGTTACGCCAAACTTGTTTGAAGCCACGCAGCTTTCTGGGCAACGTATTGAAACACTCGACGATGTCAAA
CGTGCAGCGCAAACGATTCACCAACTAGGCGCAGACCATGTTGTCATTAAGGGAGGAAGCAAGCTTAACCACCCGCAAGC
AGTTGATGTTTGGTATGACGGCCAGTCTTTCACATTGCTTGAAAATCCAGAAATCGATACGACCTACACGCACGGAGCAG
GCTGCACGTTTGCCGCAGCCATCACTGCTACGCTTGCAAAAGGCAAATCGGTTGAGGAAGCGATCCACACAGCGAAAGAC
TTTGTCACTGCCGCGATCAAGCACGGCAGCAGACTCAACGAATACGTCGGTTCCGTTGCCCACGGCGCCTACCGCCACGA
AAAACAAAACTAA

Upstream 100 bases:

>100_bases
TATCTCAGTAAAGGGAAATCATTTCCAAAAGCGCCGCTGCTTTTAAGGACGCCTATGCTTTAGTATAATGGAAAGCACGG
ACAAGGAAGGAGAGAGAAAA

Downstream 100 bases:

>100_bases
CCAGTCTCAACAAAATCATTCAGACTGATAGAAAGCGCTTGTCAGACGAGGAGTGCAGCCGAGGAAAGATGCAAATAGAA
CGTGGGTTCATAAGCAGCGG

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: PN/PL/PM kinase; Pyridoxal kinase; Pyridoxamine kinase; Vitamin B6 kinase [H]

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MERYKALTIAGSDTSGGAGIQADLKTFQELGVYGMNALTTIVAQNPFENWAHEVFPQDAKLVGKQIDTVLKGIGADAVKT
GMLGSIEIIELVANKIEEYGVNKVVVDPVMVCKGADEALNPETDACLRDVLVPKAFVVTPNLFEATQLSGQRIETLDDVK
RAAQTIHQLGADHVVIKGGSKLNHPQAVDVWYDGQSFTLLENPEIDTTYTHGAGCTFAAAITATLAKGKSVEEAIHTAKD
FVTAAIKHGSRLNEYVGSVAHGAYRHEKQN

Sequences:

>Translated_270_residues
MERYKALTIAGSDTSGGAGIQADLKTFQELGVYGMNALTTIVAQNPFENWAHEVFPQDAKLVGKQIDTVLKGIGADAVKT
GMLGSIEIIELVANKIEEYGVNKVVVDPVMVCKGADEALNPETDACLRDVLVPKAFVVTPNLFEATQLSGQRIETLDDVK
RAAQTIHQLGADHVVIKGGSKLNHPQAVDVWYDGQSFTLLENPEIDTTYTHGAGCTFAAAITATLAKGKSVEEAIHTAKD
FVTAAIKHGSRLNEYVGSVAHGAYRHEKQN
>Mature_270_residues
MERYKALTIAGSDTSGGAGIQADLKTFQELGVYGMNALTTIVAQNPFENWAHEVFPQDAKLVGKQIDTVLKGIGADAVKT
GMLGSIEIIELVANKIEEYGVNKVVVDPVMVCKGADEALNPETDACLRDVLVPKAFVVTPNLFEATQLSGQRIETLDDVK
RAAQTIHQLGADHVVIKGGSKLNHPQAVDVWYDGQSFTLLENPEIDTTYTHGAGCTFAAAITATLAKGKSVEEAIHTAKD
FVTAAIKHGSRLNEYVGSVAHGAYRHEKQN

Specific function: Phosphorylates B6 vitamers; functions in a salvage pathway. Uses pyridoxal, pyridoxine, and pyridoxamine as substrates. Can also use hydroxymethylpyrimidine (HMP) as substrate [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=264, Percent_Identity=35.9848484848485, Blast_Score=162, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6325378, Length=271, Percent_Identity=32.1033210332103, Blast_Score=129, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6324997, Length=255, Percent_Identity=32.156862745098, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6324517, Length=255, Percent_Identity=30.9803921568627, Blast_Score=99, Evalue=9e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.35 [H]

Molecular weight: Translated: 28960; Mature: 28960

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERYKALTIAGSDTSGGAGIQADLKTFQELGVYGMNALTTIVAQNPFENWAHEVFPQDAK
CCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHCCCHHH
LVGKQIDTVLKGIGADAVKTGMLGSIEIIELVANKIEEYGVNKVVVDPVMVCKGADEALN
HHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEECHHHHCCCCCCCCC
PETDACLRDVLVPKAFVVTPNLFEATQLSGQRIETLDDVKRAAQTIHQLGADHVVIKGGS
CCHHHHHHHHHCCCEEEECCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCC
KLNHPQAVDVWYDGQSFTLLENPEIDTTYTHGAGCTFAAAITATLAKGKSVEEAIHTAKD
CCCCCCEEEEEECCCEEEEEECCCCCCCEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
FVTAAIKHGSRLNEYVGSVAHGAYRHEKQN
HHHHHHHCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MERYKALTIAGSDTSGGAGIQADLKTFQELGVYGMNALTTIVAQNPFENWAHEVFPQDAK
CCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHCCCHHH
LVGKQIDTVLKGIGADAVKTGMLGSIEIIELVANKIEEYGVNKVVVDPVMVCKGADEALN
HHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEECHHHHCCCCCCCCC
PETDACLRDVLVPKAFVVTPNLFEATQLSGQRIETLDDVKRAAQTIHQLGADHVVIKGGS
CCHHHHHHHHHCCCEEEECCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCC
KLNHPQAVDVWYDGQSFTLLENPEIDTTYTHGAGCTFAAAITATLAKGKSVEEAIHTAKD
CCCCCCEEEEEECCCEEEEEECCCCCCCEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
FVTAAIKHGSRLNEYVGSVAHGAYRHEKQN
HHHHHHHCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]