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The map label for this gene is pfkA [H]

Identifier: 56965264

GI number: 56965264

Start: 3639744

End: 3640748

Strand: Direct

Name: pfkA [H]

Synonym: ABC3502

Alternate gene names: 56965264

Gene position: 3639744-3640748 (Clockwise)

Preceding gene: 56965263

Following gene: 56965265

Centisome position: 84.57

GC content: 52.04

Gene sequence:

>1005_bases
ATGTCGACGGTCCGTAACATTGCTTTAATTACAAGCGGCGGCGATGCGCCAGGGCTAAATGGTGCCATTGCGGCAATTGC
CCAAACGCCGGATGTTAACCTTTATTTCTATCATGGCGGATTTGACGGGATCATTGAGCAGGACCCTATCGCCATATCAC
CAACAACAGCGAGGGAGGCTGTGCAACAGGGAGAGTTGTTATGTTTTTCTGGCCGCAGCACACATATGCTCCATGAGGCG
GGACAAAAGCGTGTCCTCGAAAAATTAAAGCAAGACTGCATCGACGCCCTGATTGTGTGCGGTGGCAACGGCTCGGCACA
AGGGGCAAGATCGTTAAGCGCAGGCTTGCCAGTTGCCGTTGTGCCAATGACGATCGACAACGACATTGGCGGAAGTGAAT
ACACGATTGGCCATGACACAGCAGTCAATGCGATTGCCGAGTCGCTCCACCGGCTCCGCCAAACAGCAGCCAACTTGCCA
GGCCGGATTTTTATGGTCGAAACCTTTGGTGGGCGCTGCGGCCAACTTCCACTTGCTGCTGCTGTTGCTGCCAGCGCAGA
TATCGTGCTCCTTCCTGAATATGAGCTCAATATAGACAAGTTCATTACAGAAGTAAATGCCCGCTCAGCACGAGGAAAGA
GCGTCATTATTGTCGTATCTGAAGGCATCTATTTGAACAAGCGTTTTTCTGCTGGCGACCAGGGCGTGTCGTTTGCACTT
GCCCGAGCGCTTGAGGAATCAACAGGAAAGCGAGTGCGCCTCTCAATACTTGGCTACACACAACGGGCAGGTGACCCGAC
AAGCTATGACTGCCTGATGGCCAAGCAGATGGGCCAATGTGCGGTCGATGCGTTGCTTAACGGTGAAAAGGCGATGCTTG
TGGCGTTAAAGGAAGGGCGCGTCAAAGCCATTCCCCTTACAGCAATTGAACAAGCGCCTACTTTGGCGAAAGAGCTTCTG
CAATTGGCTTTTAAAGAAAACCAACTGATCCAATTGGAGGGATAA

Upstream 100 bases:

>100_bases
AAATTAATTAATGACGAACAGCGACTAGACGCAATAAAAGCCGCCACCTCCAAGCAATCGTTAACCAGAATCGTTCTCAA
CGAAGAAGGGGAGAAAGCCT

Downstream 100 bases:

>100_bases
TTATGGCAAAGAAACTATCGATTTTAATGGTGTGCGGGGCAGGGCTTGGAAGCAGTTTTGCCTGTGAGATGGCGGTGGAA
TCCGTGCTCGAAAAACTAGG

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MSTVRNIALITSGGDAPGLNGAIAAIAQTPDVNLYFYHGGFDGIIEQDPIAISPTTAREAVQQGELLCFSGRSTHMLHEA
GQKRVLEKLKQDCIDALIVCGGNGSAQGARSLSAGLPVAVVPMTIDNDIGGSEYTIGHDTAVNAIAESLHRLRQTAANLP
GRIFMVETFGGRCGQLPLAAAVAASADIVLLPEYELNIDKFITEVNARSARGKSVIIVVSEGIYLNKRFSAGDQGVSFAL
ARALEESTGKRVRLSILGYTQRAGDPTSYDCLMAKQMGQCAVDALLNGEKAMLVALKEGRVKAIPLTAIEQAPTLAKELL
QLAFKENQLIQLEG

Sequences:

>Translated_334_residues
MSTVRNIALITSGGDAPGLNGAIAAIAQTPDVNLYFYHGGFDGIIEQDPIAISPTTAREAVQQGELLCFSGRSTHMLHEA
GQKRVLEKLKQDCIDALIVCGGNGSAQGARSLSAGLPVAVVPMTIDNDIGGSEYTIGHDTAVNAIAESLHRLRQTAANLP
GRIFMVETFGGRCGQLPLAAAVAASADIVLLPEYELNIDKFITEVNARSARGKSVIIVVSEGIYLNKRFSAGDQGVSFAL
ARALEESTGKRVRLSILGYTQRAGDPTSYDCLMAKQMGQCAVDALLNGEKAMLVALKEGRVKAIPLTAIEQAPTLAKELL
QLAFKENQLIQLEG
>Mature_333_residues
STVRNIALITSGGDAPGLNGAIAAIAQTPDVNLYFYHGGFDGIIEQDPIAISPTTAREAVQQGELLCFSGRSTHMLHEAG
QKRVLEKLKQDCIDALIVCGGNGSAQGARSLSAGLPVAVVPMTIDNDIGGSEYTIGHDTAVNAIAESLHRLRQTAANLPG
RIFMVETFGGRCGQLPLAAAVAASADIVLLPEYELNIDKFITEVNARSARGKSVIIVVSEGIYLNKRFSAGDQGVSFALA
RALEESTGKRVRLSILGYTQRAGDPTSYDCLMAKQMGQCAVDALLNGEKAMLVALKEGRVKAIPLTAIEQAPTLAKELLQ
LAFKENQLIQLEG

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI266453768, Length=359, Percent_Identity=27.8551532033426, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI266453748, Length=359, Percent_Identity=27.8551532033426, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI4505749, Length=359, Percent_Identity=27.8551532033426, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI266453619, Length=361, Percent_Identity=27.7008310249307, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI11321601, Length=340, Percent_Identity=29.7058823529412, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI48762920, Length=317, Percent_Identity=28.0757097791798, Blast_Score=105, Evalue=4e-23,
Organism=Escherichia coli, GI1790350, Length=298, Percent_Identity=35.2348993288591, Blast_Score=172, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI25147584, Length=315, Percent_Identity=29.5238095238095, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI72003601, Length=304, Percent_Identity=25.6578947368421, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17558788, Length=326, Percent_Identity=27.6073619631902, Blast_Score=96, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6321679, Length=339, Percent_Identity=27.4336283185841, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6323861, Length=341, Percent_Identity=28.1524926686217, Blast_Score=109, Evalue=6e-25,
Organism=Drosophila melanogaster, GI28573326, Length=359, Percent_Identity=26.4623955431755, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24652337, Length=359, Percent_Identity=26.1838440111421, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI17647809, Length=359, Percent_Identity=26.1838440111421, Blast_Score=109, Evalue=3e-24,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 35348; Mature: 35217

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTVRNIALITSGGDAPGLNGAIAAIAQTPDVNLYFYHGGFDGIIEQDPIAISPTTAREA
CCCCCEEEEEECCCCCCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCCEEECCHHHHHH
VQQGELLCFSGRSTHMLHEAGQKRVLEKLKQDCIDALIVCGGNGSAQGARSLSAGLPVAV
HHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHCCCCEEE
VPMTIDNDIGGSEYTIGHDTAVNAIAESLHRLRQTAANLPGRIFMVETFGGRCGQLPLAA
EEEEECCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHH
AVAASADIVLLPEYELNIDKFITEVNARSARGKSVIIVVSEGIYLNKRFSAGDQGVSFAL
HHHCCCCEEEECCCCCCHHHHHHHHCCCCCCCCEEEEEEECCEEEEEECCCCCCHHHHHH
ARALEESTGKRVRLSILGYTQRAGDPTSYDCLMAKQMGQCAVDALLNGEKAMLVALKEGR
HHHHHHCCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCC
VKAIPLTAIEQAPTLAKELLQLAFKENQLIQLEG
EEEEEHHHHHHCHHHHHHHHHHHHCCCCEEEECC
>Mature Secondary Structure 
STVRNIALITSGGDAPGLNGAIAAIAQTPDVNLYFYHGGFDGIIEQDPIAISPTTAREA
CCCCEEEEEECCCCCCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCCEEECCHHHHHH
VQQGELLCFSGRSTHMLHEAGQKRVLEKLKQDCIDALIVCGGNGSAQGARSLSAGLPVAV
HHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHCCCCEEE
VPMTIDNDIGGSEYTIGHDTAVNAIAESLHRLRQTAANLPGRIFMVETFGGRCGQLPLAA
EEEEECCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHH
AVAASADIVLLPEYELNIDKFITEVNARSARGKSVIIVVSEGIYLNKRFSAGDQGVSFAL
HHHCCCCEEEECCCCCCHHHHHHHHCCCCCCCCEEEEEEECCEEEEEECCCCCCHHHHHH
ARALEESTGKRVRLSILGYTQRAGDPTSYDCLMAKQMGQCAVDALLNGEKAMLVALKEGR
HHHHHHCCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCC
VKAIPLTAIEQAPTLAKELLQLAFKENQLIQLEG
EEEEEHHHHHHCHHHHHHHHHHHHCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA