Click here to switch to the map view.

The map label for this gene is yqiI [H]

Identifier: 56965210

GI number: 56965210

Start: 3578075

End: 3578833

Strand: Direct

Name: yqiI [H]

Synonym: ABC3448

Alternate gene names: 56965210

Gene position: 3578075-3578833 (Clockwise)

Preceding gene: 56965207

Following gene: 56965213

Centisome position: 83.14

GC content: 46.38

Gene sequence:

>759_bases
ATGTTTTTCTCTTTGGATACATATTTTCCCTTCATTTTAATCAGGAAGGGCTGTTTTCGACAAAATCCCTTGTTGCCTCT
ATGGCAATCTCTTAGACTACTAATAAGCCAAGCGATAAAAACCAAGGAGGCGCTCATGAGAAAAAAGCATGCTTTTTCAT
TTTTGATGGCCGCCATCCTTGCAGTAGTCTCCACTAGCTATTTTCTAGCATCAGAAGCGGCGGCTAGTGTATCTGGTAAA
GTCATTGCCATTGATGCGGGCCACGGTGGAAGCGATCCTGGGGCAGTCGCAAATGGAATTTCCGAGAAAGATCTTGTTTT
AAAAGTAGCTGCGCATACGAAAGATAGGCTGGAAGAGGCAGGGGCAACAGTCATTATGACGAGAACAGGGGACGTATATG
TTGGGCTCGAAGCCAGGGCCGAACTTGCTAATGCCCGTAATGCCGATACATTTGTTAGCATTCATGCCAATGCAGCTACC
CCAAGTGCCCATGGGACAGAAACATTCCACTTCCCATCAAGCAGCCAAGGACAAGCTTTGGCTTCAGCTTTGCAAACAGA
ACTCGTCAACACACTAAATACAAGAAACCGCGGCGTCAAATCGGCAAATTTTAGTGTATTGCGCAATACTGCCATGCCTG
CGGCCCTTGTCGAACTTGGATTTATTACAAATGCTGAAGAAGCAGAACGGATGAAAGCCGCCAGCTTTCCAAACGAAGCT
GCAACAGCGATTGTACGCGGGCTCCAACAATACCATTAA

Upstream 100 bases:

>100_bases
GCTAAACAGTTTCCTAACTGGGCGATCTTGAACCGTTACTAGTCACCTCTGTTCAGACGCTTTTCCTTGCTTTCATAAAA
CTTAACCACTGTGATTCTTC

Downstream 100 bases:

>100_bases
AAGCATGACCTGTTCCAGCAAGCCTTTGTACAAACAGAGACGCATCTATCTCGTTTTGCCTAAGTCTTCGCACAGCTTAG
TGTCTGCACAACCAAAAAAG

Product: hypothetical protein

Products: Hydrolyzed cell wall glycopeptides; N-acetylmuramoyl residues; L-amino acid residues [C]

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MFFSLDTYFPFILIRKGCFRQNPLLPLWQSLRLLISQAIKTKEALMRKKHAFSFLMAAILAVVSTSYFLASEAAASVSGK
VIAIDAGHGGSDPGAVANGISEKDLVLKVAAHTKDRLEEAGATVIMTRTGDVYVGLEARAELANARNADTFVSIHANAAT
PSAHGTETFHFPSSSQGQALASALQTELVNTLNTRNRGVKSANFSVLRNTAMPAALVELGFITNAEEAERMKAASFPNEA
ATAIVRGLQQYH

Sequences:

>Translated_252_residues
MFFSLDTYFPFILIRKGCFRQNPLLPLWQSLRLLISQAIKTKEALMRKKHAFSFLMAAILAVVSTSYFLASEAAASVSGK
VIAIDAGHGGSDPGAVANGISEKDLVLKVAAHTKDRLEEAGATVIMTRTGDVYVGLEARAELANARNADTFVSIHANAAT
PSAHGTETFHFPSSSQGQALASALQTELVNTLNTRNRGVKSANFSVLRNTAMPAALVELGFITNAEEAERMKAASFPNEA
ATAIVRGLQQYH
>Mature_252_residues
MFFSLDTYFPFILIRKGCFRQNPLLPLWQSLRLLISQAIKTKEALMRKKHAFSFLMAAILAVVSTSYFLASEAAASVSGK
VIAIDAGHGGSDPGAVANGISEKDLVLKVAAHTKDRLEEAGATVIMTRTGDVYVGLEARAELANARNADTFVSIHANAAT
PSAHGTETFHFPSSSQGQALASALQTELVNTLNTRNRGVKSANFSVLRNTAMPAALVELGFITNAEEAERMKAASFPNEA
ATAIVRGLQQYH

Specific function: Unknown

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=227, Percent_Identity=30.8370044052863, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI87082163, Length=220, Percent_Identity=31.3636363636364, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1790611, Length=235, Percent_Identity=28.0851063829787, Blast_Score=81, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: 3.5.1.28 [C]

Molecular weight: Translated: 26996; Mature: 26996

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFSLDTYFPFILIRKGCFRQNPLLPLWQSLRLLISQAIKTKEALMRKKHAFSFLMAAIL
CEECCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVVSTSYFLASEAAASVSGKVIAIDAGHGGSDPGAVANGISEKDLVLKVAAHTKDRLEEA
HHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHCCCCCCHHEEEHHHHHHHHHHHC
GATVIMTRTGDVYVGLEARAELANARNADTFVSIHANAATPSAHGTETFHFPSSSQGQAL
CCEEEEEECCCEEEEHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCEEEECCCCCCHHHH
ASALQTELVNTLNTRNRGVKSANFSVLRNTAMPAALVELGFITNAEEAERMKAASFPNEA
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHCCCHHH
ATAIVRGLQQYH
HHHHHHHHHHCC
>Mature Secondary Structure
MFFSLDTYFPFILIRKGCFRQNPLLPLWQSLRLLISQAIKTKEALMRKKHAFSFLMAAIL
CEECCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVVSTSYFLASEAAASVSGKVIAIDAGHGGSDPGAVANGISEKDLVLKVAAHTKDRLEEA
HHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHCCCCCCHHEEEHHHHHHHHHHHC
GATVIMTRTGDVYVGLEARAELANARNADTFVSIHANAATPSAHGTETFHFPSSSQGQAL
CCEEEEEECCCEEEEHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCEEEECCCCCCHHHH
ASALQTELVNTLNTRNRGVKSANFSVLRNTAMPAALVELGFITNAEEAERMKAASFPNEA
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHCCCHHH
ATAIVRGLQQYH
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: cell wall glycopeptides; D-lactyl-L-Ala [C]

Specific reaction: Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]