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The map label for this gene is adaB [H]

Identifier: 56964918

GI number: 56964918

Start: 3279447

End: 3279980

Strand: Direct

Name: adaB [H]

Synonym: ABC3154

Alternate gene names: 56964918

Gene position: 3279447-3279980 (Clockwise)

Preceding gene: 56964917

Following gene: 56964925

Centisome position: 76.2

GC content: 43.63

Gene sequence:

>534_bases
ATGGAATCAAACAACAAACCAATCCTTTATTGGTCTTTGCTAACGTTTAGAGATTTGAATTTTTATATCGCTTCAACCTC
TAAGGGACTTGTGTTTGTTGGCTCACCGAACAAACCATTCGAGGAATTGTCCGAATGGGCTAAGAAGCGCTTTAGCAGAA
GTCCTTTGGTGGAAGCAGCTGAAAAGCTTGAGCCCTATGCGGTTGAAATCACCGAGTATTTAGAAGGAAAACGGAAAACC
TTTACGGTTTCATTTGATTACGATGGAACAGCATTCCAGCTTGCAGTCTGGAACGCACTTTGTGCAATTCCGTACGGACA
AACTAGGTCCTATTCCGACATTGCCAATTCTATAAATAAACCAGCAGCCGTTCGAGCTGTAGGGGCGGCGATTGGAGCGA
ATCCAGTATTAATCACCGTACCATGTCATCGTGTAGTAGGAAAAAATGGATCATTAACTGGCTACCGCGGTGGATTCGAA
ATGAAGACACGGCTTTTGGATTTGGAAAGACAAGGCTTATCGAGCAATGAGTGA

Upstream 100 bases:

>100_bases
GGTATGCCTAACACGTCCTATTTTATTACTTTATTTAAAAAGAAAATGGGACATACACCAGCACAATTCCGTCAAATCAG
TAAAATGGAGGAACGGTAAA

Downstream 100 bases:

>100_bases
TTAGTGAAAGGAGGTTCCGTCACGGGAATCCTAGCCCTACCGTAAATTAGGTAGAGTGGAATAAAACAAAAAAATCGCCG
CTCTAATTTTGCGGGAATGA

Product: methylated-DNA-[protein]-cysteine S-methyltransferase

Products: NA

Alternate protein names: O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 177; Mature: 177

Protein sequence:

>177_residues
MESNNKPILYWSLLTFRDLNFYIASTSKGLVFVGSPNKPFEELSEWAKKRFSRSPLVEAAEKLEPYAVEITEYLEGKRKT
FTVSFDYDGTAFQLAVWNALCAIPYGQTRSYSDIANSINKPAAVRAVGAAIGANPVLITVPCHRVVGKNGSLTGYRGGFE
MKTRLLDLERQGLSSNE

Sequences:

>Translated_177_residues
MESNNKPILYWSLLTFRDLNFYIASTSKGLVFVGSPNKPFEELSEWAKKRFSRSPLVEAAEKLEPYAVEITEYLEGKRKT
FTVSFDYDGTAFQLAVWNALCAIPYGQTRSYSDIANSINKPAAVRAVGAAIGANPVLITVPCHRVVGKNGSLTGYRGGFE
MKTRLLDLERQGLSSNE
>Mature_177_residues
MESNNKPILYWSLLTFRDLNFYIASTSKGLVFVGSPNKPFEELSEWAKKRFSRSPLVEAAEKLEPYAVEITEYLEGKRKT
FTVSFDYDGTAFQLAVWNALCAIPYGQTRSYSDIANSINKPAAVRAVGAAIGANPVLITVPCHRVVGKNGSLTGYRGGFE
MKTRLLDLERQGLSSNE

Specific function: Repair of alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction:the enzyme is irreversibly inactivated [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=137, Percent_Identity=29.9270072992701, Blast_Score=66, Evalue=2e-11,
Organism=Escherichia coli, GI1788542, Length=107, Percent_Identity=45.7943925233645, Blast_Score=100, Evalue=5e-23,
Organism=Escherichia coli, GI1787596, Length=102, Percent_Identity=46.078431372549, Blast_Score=96, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI115533070, Length=101, Percent_Identity=47.5247524752475, Blast_Score=90, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI115533068, Length=101, Percent_Identity=47.5247524752475, Blast_Score=90, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6320001, Length=168, Percent_Identity=34.5238095238095, Blast_Score=89, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 19653; Mature: 19653

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESNNKPILYWSLLTFRDLNFYIASTSKGLVFVGSPNKPFEELSEWAKKRFSRSPLVEAA
CCCCCCCEEEEEEEEEECCEEEEEECCCCEEEECCCCCCHHHHHHHHHHHCCCCHHHHHH
EKLEPYAVEITEYLEGKRKTFTVSFDYDGTAFQLAVWNALCAIPYGQTRSYSDIANSINK
HHCCCEEEEHHHHHCCCCEEEEEEECCCCCEEEHEEHHHHHCCCCCCCCCHHHHHHHCCC
PAAVRAVGAAIGANPVLITVPCHRVVGKNGSLTGYRGGFEMKTRLLDLERQGLSSNE
CHHHHHHHHHHCCCCEEEEECCHHHHCCCCCCCEECCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MESNNKPILYWSLLTFRDLNFYIASTSKGLVFVGSPNKPFEELSEWAKKRFSRSPLVEAA
CCCCCCCEEEEEEEEEECCEEEEEECCCCEEEECCCCCCHHHHHHHHHHHCCCCHHHHHH
EKLEPYAVEITEYLEGKRKTFTVSFDYDGTAFQLAVWNALCAIPYGQTRSYSDIANSINK
HHCCCEEEEHHHHHCCCCEEEEEEECCCCCEEEHEEHHHHHCCCCCCCCCHHHHHHHCCC
PAAVRAVGAAIGANPVLITVPCHRVVGKNGSLTGYRGGFEMKTRLLDLERQGLSSNE
CHHHHHHHHHHCCCCEEEEECCHHHHCCCCCCCEECCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2120677; 9384377; 1744039 [H]