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The map label for this gene is nrdE [H]

Identifier: 56964872

GI number: 56964872

Start: 3234374

End: 3236470

Strand: Direct

Name: nrdE [H]

Synonym: ABC3108

Alternate gene names: 56964872

Gene position: 3234374-3236470 (Clockwise)

Preceding gene: 56964871

Following gene: 56964873

Centisome position: 75.15

GC content: 44.4

Gene sequence:

>2097_bases
TTGGCACAAGTTCCAAAGTGGGTTCAGCTTAACAACGAGCTAATGATTACAAAGGACGGCGGCTACCAGTTTGAAAAGGA
CAAAGAAGCTGTACATAGCTATTTCGTAGACTACATTAACCAAAACACCGTCTTTTTCCATGATCTTGAAGAAAAGATCG
ACTACTTAATTGAAAATGATTATTACGAAGAAGAGCTTCTGCGCTTGTATACGATCGACGAAATTAAAGCAGTGATGGAC
ATTGCCTATAACAAAAAATTCCGTTTCCCGTCGTTCATGAGCGCTTTTAAATTTTACAATGATTACGCGCTAAAAACGAA
TGACAAAAGCAAAATTTTAGAGCGATATGAAGACCGTGTCGCCATTGTTTCCCTTTTCTTCGGAAACGGGGATGTTGAAA
AAGCCAAAGCGTTAGTAAATGCCATGATTTCGCAAGAGTTCCAGCCAGCGACGCCCACGTTCCTCAATGCAGGACGTAAA
CGGCGCGGCGAACTCGTTTCCTGCTTCTTGCTTGAAATTAACGATTCATTAAATGACATTAGCCGAGCTGTCGACATTTC
AATGCAGCTCTCAAAGCTTGGCGGCGGTGTGGCCCTTAACTTATCAAAGCTACGCGCGAAAGGCGAGGCAATCAAAGACG
TGGCTAACGCAACAAAAGGCGTTGTCGGCGTCATGAAACTTCTCGACAATGCGTTTCGCTATGCCGACCAAATGGGGCAG
CGCCAAGGGTCTGGTGCAGCGTACTTAAATATTTTCCACGCTGACATTAACGACTTTCTTGACACGCGGAAAATTTCTGC
TGATGAAGACGTACGCGTTAAAACATTGTCGATTGGCGTCGTTATCCCTGACAAGTTTATTGAGTTGATGCGCGAAGATA
AAGATGCATACTTGTTTTATCCACATACTGTTTATAAAGCTTACGGCCAACATTTTGATGAAATGGACATGGAAGAAATG
TACGAAACGCTTGTTGACGACCCGCGGGTACGGAAAGAAAAAATCAACCCTCGTCGTCTGTTCCAAAAGCTGGCGATCTT
GCGGTCCGAATCCGGCTACCCATACATTATGTTTGCCGATAACGTGAACAAGGCCCATGCAAATGGCCATATTAGTAATG
TCAAGTTTTCAAACTTATGTTCGGAAATCTTACAAGCCTCAGAAGTATCGACTTACACCGACTACGGCGAGGAAGATGAA
ATCGGCCTTGACATTTCCTGCAACCTTGGGTCGATTAACATTATGAATGTGATGGAAAACAAATCGCTTGAAAACACGGT
TAGCCTGGCGACAGACGCTTTAACGCGCGTATCAGAGTCAACCAATATTACAAATGCGCCAGCTGTGCGCCGCGGTAACA
AACTGATGAAATCAATCGGCCTAGGCGCAATGAATTTGCACGGTTACCTAGCGAAGCAGCATATTGCTTACGAAAGCGAA
GAAGCTCGTGATTTTGCCAATACCTTTTTTATGGCGATGAACTATTTCTCTATTAAACGGTCTGCTGAGATTGCCAGGGA
CCAAGGTGAAACGTATCACGGTTATGAAGGCAGCACGTACGCTGACGGCTCCTACTTTAAAAAGTATATCGAAAATGACT
TCACGCCTAAATTTGACAAAGTCAAAGCGCTGTTTGACGGCATGTTTGTGCCAACAAAAGAAGATTGGCAAGCTTTGCAA
GCCTTTGTTGCAGAACACGGCCTTTACCATAGTTACCGATTGGCGATCGCGCCAACTGGTTCGATTTCTTATGTACAGTC
GGCCACGGCAAGCGTCATGCCGATCATGGAACGGATTGAAGAACGGACATACGGCAACTCCAAAACATACTATCCAATGC
CTGGGCTTAATGCGAACAATTGGTTCTTCTACAAAGAAGCCTATGACATGGATATGTTTAAAGTCGTTGATATGATTGCA
ACGATCCAACAACACGTCGATCAAGGGATCAGCTTTACGCTTTTCCTAAAGGATACGATGACGACCCGTGATTTGAACCG
AATCGACCTTTATGCGCACCATAAAGGCATCAAAACCCTTTACTACGCGCGCACAAAAGATACAGGGCAAGACAACTGCC
TTTCTTGTGTCGTTTAA

Upstream 100 bases:

>100_bases
GACACCATCGCAAACATGTACAATGTTCCTGTTCTTCATAAATTCGAGCTAAGCGGAACGTCTAGAGACGTCGACGCTTT
TTTACAGGGGGTAAATTCAA

Downstream 100 bases:

>100_bases
CAAAGTTTAAATGCAACCTAAAAAGGAGAGTGCCTAATGACGCACGTCTACGATGCGGCCAACTGGTCCAAGCATGAAGA
TGATTTTACGCAAATGTTTT

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: Ribonucleotide reductase large subunit [H]

Number of amino acids: Translated: 698; Mature: 697

Protein sequence:

>698_residues
MAQVPKWVQLNNELMITKDGGYQFEKDKEAVHSYFVDYINQNTVFFHDLEEKIDYLIENDYYEEELLRLYTIDEIKAVMD
IAYNKKFRFPSFMSAFKFYNDYALKTNDKSKILERYEDRVAIVSLFFGNGDVEKAKALVNAMISQEFQPATPTFLNAGRK
RRGELVSCFLLEINDSLNDISRAVDISMQLSKLGGGVALNLSKLRAKGEAIKDVANATKGVVGVMKLLDNAFRYADQMGQ
RQGSGAAYLNIFHADINDFLDTRKISADEDVRVKTLSIGVVIPDKFIELMREDKDAYLFYPHTVYKAYGQHFDEMDMEEM
YETLVDDPRVRKEKINPRRLFQKLAILRSESGYPYIMFADNVNKAHANGHISNVKFSNLCSEILQASEVSTYTDYGEEDE
IGLDISCNLGSINIMNVMENKSLENTVSLATDALTRVSESTNITNAPAVRRGNKLMKSIGLGAMNLHGYLAKQHIAYESE
EARDFANTFFMAMNYFSIKRSAEIARDQGETYHGYEGSTYADGSYFKKYIENDFTPKFDKVKALFDGMFVPTKEDWQALQ
AFVAEHGLYHSYRLAIAPTGSISYVQSATASVMPIMERIEERTYGNSKTYYPMPGLNANNWFFYKEAYDMDMFKVVDMIA
TIQQHVDQGISFTLFLKDTMTTRDLNRIDLYAHHKGIKTLYYARTKDTGQDNCLSCVV

Sequences:

>Translated_698_residues
MAQVPKWVQLNNELMITKDGGYQFEKDKEAVHSYFVDYINQNTVFFHDLEEKIDYLIENDYYEEELLRLYTIDEIKAVMD
IAYNKKFRFPSFMSAFKFYNDYALKTNDKSKILERYEDRVAIVSLFFGNGDVEKAKALVNAMISQEFQPATPTFLNAGRK
RRGELVSCFLLEINDSLNDISRAVDISMQLSKLGGGVALNLSKLRAKGEAIKDVANATKGVVGVMKLLDNAFRYADQMGQ
RQGSGAAYLNIFHADINDFLDTRKISADEDVRVKTLSIGVVIPDKFIELMREDKDAYLFYPHTVYKAYGQHFDEMDMEEM
YETLVDDPRVRKEKINPRRLFQKLAILRSESGYPYIMFADNVNKAHANGHISNVKFSNLCSEILQASEVSTYTDYGEEDE
IGLDISCNLGSINIMNVMENKSLENTVSLATDALTRVSESTNITNAPAVRRGNKLMKSIGLGAMNLHGYLAKQHIAYESE
EARDFANTFFMAMNYFSIKRSAEIARDQGETYHGYEGSTYADGSYFKKYIENDFTPKFDKVKALFDGMFVPTKEDWQALQ
AFVAEHGLYHSYRLAIAPTGSISYVQSATASVMPIMERIEERTYGNSKTYYPMPGLNANNWFFYKEAYDMDMFKVVDMIA
TIQQHVDQGISFTLFLKDTMTTRDLNRIDLYAHHKGIKTLYYARTKDTGQDNCLSCVV
>Mature_697_residues
AQVPKWVQLNNELMITKDGGYQFEKDKEAVHSYFVDYINQNTVFFHDLEEKIDYLIENDYYEEELLRLYTIDEIKAVMDI
AYNKKFRFPSFMSAFKFYNDYALKTNDKSKILERYEDRVAIVSLFFGNGDVEKAKALVNAMISQEFQPATPTFLNAGRKR
RGELVSCFLLEINDSLNDISRAVDISMQLSKLGGGVALNLSKLRAKGEAIKDVANATKGVVGVMKLLDNAFRYADQMGQR
QGSGAAYLNIFHADINDFLDTRKISADEDVRVKTLSIGVVIPDKFIELMREDKDAYLFYPHTVYKAYGQHFDEMDMEEMY
ETLVDDPRVRKEKINPRRLFQKLAILRSESGYPYIMFADNVNKAHANGHISNVKFSNLCSEILQASEVSTYTDYGEEDEI
GLDISCNLGSINIMNVMENKSLENTVSLATDALTRVSESTNITNAPAVRRGNKLMKSIGLGAMNLHGYLAKQHIAYESEE
ARDFANTFFMAMNYFSIKRSAEIARDQGETYHGYEGSTYADGSYFKKYIENDFTPKFDKVKALFDGMFVPTKEDWQALQA
FVAEHGLYHSYRLAIAPTGSISYVQSATASVMPIMERIEERTYGNSKTYYPMPGLNANNWFFYKEAYDMDMFKVVDMIAT
IQQHVDQGISFTLFLKDTMTTRDLNRIDLYAHHKGIKTLYYARTKDTGQDNCLSCVV

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase large chain family [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=692, Percent_Identity=25.5780346820809, Blast_Score=193, Evalue=4e-49,
Organism=Escherichia coli, GI1789030, Length=687, Percent_Identity=44.6870451237263, Blast_Score=621, Evalue=1e-179,
Organism=Escherichia coli, GI2367133, Length=660, Percent_Identity=24.8484848484848, Blast_Score=162, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI17555242, Length=649, Percent_Identity=26.6563944530046, Blast_Score=207, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6322123, Length=655, Percent_Identity=25.9541984732824, Blast_Score=204, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6320914, Length=655, Percent_Identity=25.8015267175572, Blast_Score=196, Evalue=1e-50,
Organism=Drosophila melanogaster, GI17352481, Length=654, Percent_Identity=27.0642201834862, Blast_Score=202, Evalue=9e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926
- InterPro:   IPR013554 [H]

Pfam domain/function: PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN; PF08343 RNR_N [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 79770; Mature: 79639

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00089 RIBORED_LARGE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQVPKWVQLNNELMITKDGGYQFEKDKEAVHSYFVDYINQNTVFFHDLEEKIDYLIEND
CCCCCCHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHCCC
YYEEELLRLYTIDEIKAVMDIAYNKKFRFPSFMSAFKFYNDYALKTNDKSKILERYEDRV
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEECCCHHHHHHHHHCCE
AIVSLFFGNGDVEKAKALVNAMISQEFQPATPTFLNAGRKRRGELVSCFLLEINDSLNDI
EEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCHHHHCCHHHCCCEEEEEEEEECCCHHHH
SRAVDISMQLSKLGGGVALNLSKLRAKGEAIKDVANATKGVVGVMKLLDNAFRYADQMGQ
HHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RQGSGAAYLNIFHADINDFLDTRKISADEDVRVKTLSIGVVIPDKFIELMREDKDAYLFY
CCCCCEEEEEEEECCHHHHHHHCCCCCCCCCEEEEEEEEEEECHHHHHHHHCCCCCEEEC
PHTVYKAYGQHFDEMDMEEMYETLVDDPRVRKEKINPRRLFQKLAILRSESGYPYIMFAD
CHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHCCCCCEEEEEEC
NVNKAHANGHISNVKFSNLCSEILQASEVSTYTDYGEEDEIGLDISCNLGSINIMNVMEN
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHCCCCCCCCCCEEEEECCCCEEEEEHHHC
KSLENTVSLATDALTRVSESTNITNAPAVRRGNKLMKSIGLGAMNLHGYLAKQHIAYESE
CCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCH
EARDFANTFFMAMNYFSIKRSAEIARDQGETYHGYEGSTYADGSYFKKYIENDFTPKFDK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHH
VKALFDGMFVPTKEDWQALQAFVAEHGLYHSYRLAIAPTGSISYVQSATASVMPIMERIE
HHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
ERTYGNSKTYYPMPGLNANNWFFYKEAYDMDMFKVVDMIATIQQHVDQGISFTLFLKDTM
HHCCCCCCEECCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
TTRDLNRIDLYAHHKGIKTLYYARTKDTGQDNCLSCVV
CCCCCCCEEEEECCCCCCEEEEEECCCCCCCCHHEECC
>Mature Secondary Structure 
AQVPKWVQLNNELMITKDGGYQFEKDKEAVHSYFVDYINQNTVFFHDLEEKIDYLIEND
CCCCCHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHCCC
YYEEELLRLYTIDEIKAVMDIAYNKKFRFPSFMSAFKFYNDYALKTNDKSKILERYEDRV
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEECCCHHHHHHHHHCCE
AIVSLFFGNGDVEKAKALVNAMISQEFQPATPTFLNAGRKRRGELVSCFLLEINDSLNDI
EEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCHHHHCCHHHCCCEEEEEEEEECCCHHHH
SRAVDISMQLSKLGGGVALNLSKLRAKGEAIKDVANATKGVVGVMKLLDNAFRYADQMGQ
HHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RQGSGAAYLNIFHADINDFLDTRKISADEDVRVKTLSIGVVIPDKFIELMREDKDAYLFY
CCCCCEEEEEEEECCHHHHHHHCCCCCCCCCEEEEEEEEEEECHHHHHHHHCCCCCEEEC
PHTVYKAYGQHFDEMDMEEMYETLVDDPRVRKEKINPRRLFQKLAILRSESGYPYIMFAD
CHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHCCCCCEEEEEEC
NVNKAHANGHISNVKFSNLCSEILQASEVSTYTDYGEEDEIGLDISCNLGSINIMNVMEN
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHCCCCCCCCCCEEEEECCCCEEEEEHHHC
KSLENTVSLATDALTRVSESTNITNAPAVRRGNKLMKSIGLGAMNLHGYLAKQHIAYESE
CCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCH
EARDFANTFFMAMNYFSIKRSAEIARDQGETYHGYEGSTYADGSYFKKYIENDFTPKFDK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHH
VKALFDGMFVPTKEDWQALQAFVAEHGLYHSYRLAIAPTGSISYVQSATASVMPIMERIE
HHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
ERTYGNSKTYYPMPGLNANNWFFYKEAYDMDMFKVVDMIATIQQHVDQGISFTLFLKDTM
HHCCCCCCEECCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
TTRDLNRIDLYAHHKGIKTLYYARTKDTGQDNCLSCVV
CCCCCCCEEEEECCCCCCEEEEEECCCCCCCCHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969495; 9384377 [H]