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The map label for this gene is dapE [H]

Identifier: 56964791

GI number: 56964791

Start: 3149366

End: 3150664

Strand: Direct

Name: dapE [H]

Synonym: ABC3027

Alternate gene names: 56964791

Gene position: 3149366-3150664 (Clockwise)

Preceding gene: 56964790

Following gene: 56964793

Centisome position: 73.18

GC content: 47.88

Gene sequence:

>1299_bases
TTGGCTACGTTGTTTAAACTGTTGCGCCAAGAAAGCATTAGCACTCAAAACAAAGGAATGCGCGAATGCGCAGCGTTAAT
GCGATCGCTTATGGAGGAAGTCGGCGTCTGTACTAGACTGTTTGAAACGAAGGGGCACCCTATTTTATACGGTGAACTTC
TCACAGACAAACACGCGCCAACTTTGTTAATTTATGGCCATTACGATGTACAGCCTCCTGACCCATTAAGTGAGTGGGAG
ACGCCTCCATTTGAACCAACAGTCCGCGACGGGCGTATCTTTGCACGGGGCGCAGGCGACAACAAAGGACAAATCGTTGC
CCAACTTCTCGGCATAAAAACGTATCAAGAAGCATGTGGCGCTTTACCCGTCAACATTAAAATCGTTATTGAAGGGGAAG
AAGAAATGGGCAGCGTCCATTTGCCAGACTTTGTTCAAAGCAACAAAGAATTGCTGCAAGCTGATCTCGTTTACACAGCT
GATGGGCCTTCTCATGAAAGCAGCTCTCCCCTTGTCCTTCTTGGCGTCCGCGGCATCCTTTCTTTTGAAATTGAACTACA
AAACGCCGAGTTCGACAACCATTCTGGCAATACAGGCAACATTGTCCCAAATCCAGCTTGGGACCTTATGGAGCTGCTGC
AGACGATGCGCAGCACAGACGGGCGCGTCTTAATCGAAGGCTTTTACGACAATATTCGCCCTGCCAGCGCTGAAGAAGAA
CAGCTGTTGGCCACACTCCCTTTTGAAAAAGACTCAGTCGCCCAAAAAATCGGCTATCCCCATTTGGATATTGACGGAAA
AAGCTATTACCGAAAACTAGCGCTAGAACCCACTTTTAATATATGCGGACTAAAAAGCGGCTATTTAGAAGATGGCATTA
AGACAATTATTCCTAGCAAAGCCAGTGTAAAAATCGACGCACGCCTCGTTGTGGACCAGGACCCTAAAGACATTTTTGAA
AAGGTGACTGCACACGTAAAAGCACGCCGGCCTGATGCCAAGGTGACGTTTTTAGGGGCAATGGAGCCTTCCCGTACGCC
AGTTGAGACAGCGATCGTCCAAAAAGCGCTAAAAGGCATTTCTGCTTGTTTTAATGAGGAGCCCCTTATCCAACCAAGCC
TTGGCGGCTCGCTGCCAGACTATGTATGGACAAAGCTCCTTCACGCACCATCTCTTCTCGTCCCTTATGCAAACTTTGAC
CAACGCAACCACTCGCCAAATGAAAATCTCGCTATTCGCCACTTTTTAAACGGCATTCGCTGTACAGCCCATGTTATCCA
TGCTGTCGGACAGAACTAA

Upstream 100 bases:

>100_bases
CGATGGCGCTAGCCTTTTTGCTTTCGCCTAAGACTAGGAGGAATTTATTCTGAGCATTCCAGCAATTGACGACATTGTCG
CCAAGCAAACAGACGATTCA

Downstream 100 bases:

>100_bases
TAAAAAAGGCATCCCTCATTATGGGATGCCTCCAGACTGTAGGCAAACGCTCGCATACGTCGTCGTTTGCCTCAGTCATA
TGCTCATGAACCCACGTTCT

Product: deacylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MATLFKLLRQESISTQNKGMRECAALMRSLMEEVGVCTRLFETKGHPILYGELLTDKHAPTLLIYGHYDVQPPDPLSEWE
TPPFEPTVRDGRIFARGAGDNKGQIVAQLLGIKTYQEACGALPVNIKIVIEGEEEMGSVHLPDFVQSNKELLQADLVYTA
DGPSHESSSPLVLLGVRGILSFEIELQNAEFDNHSGNTGNIVPNPAWDLMELLQTMRSTDGRVLIEGFYDNIRPASAEEE
QLLATLPFEKDSVAQKIGYPHLDIDGKSYYRKLALEPTFNICGLKSGYLEDGIKTIIPSKASVKIDARLVVDQDPKDIFE
KVTAHVKARRPDAKVTFLGAMEPSRTPVETAIVQKALKGISACFNEEPLIQPSLGGSLPDYVWTKLLHAPSLLVPYANFD
QRNHSPNENLAIRHFLNGIRCTAHVIHAVGQN

Sequences:

>Translated_432_residues
MATLFKLLRQESISTQNKGMRECAALMRSLMEEVGVCTRLFETKGHPILYGELLTDKHAPTLLIYGHYDVQPPDPLSEWE
TPPFEPTVRDGRIFARGAGDNKGQIVAQLLGIKTYQEACGALPVNIKIVIEGEEEMGSVHLPDFVQSNKELLQADLVYTA
DGPSHESSSPLVLLGVRGILSFEIELQNAEFDNHSGNTGNIVPNPAWDLMELLQTMRSTDGRVLIEGFYDNIRPASAEEE
QLLATLPFEKDSVAQKIGYPHLDIDGKSYYRKLALEPTFNICGLKSGYLEDGIKTIIPSKASVKIDARLVVDQDPKDIFE
KVTAHVKARRPDAKVTFLGAMEPSRTPVETAIVQKALKGISACFNEEPLIQPSLGGSLPDYVWTKLLHAPSLLVPYANFD
QRNHSPNENLAIRHFLNGIRCTAHVIHAVGQN
>Mature_431_residues
ATLFKLLRQESISTQNKGMRECAALMRSLMEEVGVCTRLFETKGHPILYGELLTDKHAPTLLIYGHYDVQPPDPLSEWET
PPFEPTVRDGRIFARGAGDNKGQIVAQLLGIKTYQEACGALPVNIKIVIEGEEEMGSVHLPDFVQSNKELLQADLVYTAD
GPSHESSSPLVLLGVRGILSFEIELQNAEFDNHSGNTGNIVPNPAWDLMELLQTMRSTDGRVLIEGFYDNIRPASAEEEQ
LLATLPFEKDSVAQKIGYPHLDIDGKSYYRKLALEPTFNICGLKSGYLEDGIKTIIPSKASVKIDARLVVDQDPKDIFEK
VTAHVKARRPDAKVTFLGAMEPSRTPVETAIVQKALKGISACFNEEPLIQPSLGGSLPDYVWTKLLHAPSLLVPYANFDQ
RNHSPNENLAIRHFLNGIRCTAHVIHAVGQN

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Homo sapiens, GI21071039, Length=396, Percent_Identity=28.2828282828283, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI271398239, Length=398, Percent_Identity=27.6381909547739, Blast_Score=162, Evalue=7e-40,
Organism=Homo sapiens, GI271398277, Length=323, Percent_Identity=25.3869969040248, Blast_Score=104, Evalue=2e-22,
Organism=Escherichia coli, GI1788816, Length=189, Percent_Identity=26.984126984127, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17563146, Length=400, Percent_Identity=30.75, Blast_Score=183, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI193211059, Length=451, Percent_Identity=28.159645232816, Blast_Score=160, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6321159, Length=404, Percent_Identity=26.980198019802, Blast_Score=143, Evalue=6e-35,
Organism=Saccharomyces cerevisiae, GI6319758, Length=433, Percent_Identity=23.5565819861432, Blast_Score=113, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24585880, Length=397, Percent_Identity=32.7455919395466, Blast_Score=188, Evalue=7e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 47787; Mature: 47656

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATLFKLLRQESISTQNKGMRECAALMRSLMEEVGVCTRLFETKGHPILYGELLTDKHAP
CHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHCCCCCC
TLLIYGHYDVQPPDPLSEWETPPFEPTVRDGRIFARGAGDNKGQIVAQLLGIKTYQEACG
EEEEEEECCCCCCCCCHHCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHC
ALPVNIKIVIEGEEEMGSVHLPDFVQSNKELLQADLVYTADGPSHESSSPLVLLGVRGIL
CCCEEEEEEEECHHHCCCCCCCHHHHCCHHHHHHHEEEECCCCCCCCCCCEEEEEECCEE
SFEIELQNAEFDNHSGNTGNIVPNPAWDLMELLQTMRSTDGRVLIEGFYDNIRPASAEEE
EEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHH
QLLATLPFEKDSVAQKIGYPHLDIDGKSYYRKLALEPTFNICGLKSGYLEDGIKTIIPSK
HEEEECCCCHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCHHHCCHHHHCCCC
ASVKIDARLVVDQDPKDIFEKVTAHVKARRPDAKVTFLGAMEPSRTPVETAIVQKALKGI
CCEEEEEEEEECCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHH
SACFNEEPLIQPSLGGSLPDYVWTKLLHAPSLLVPYANFDQRNHSPNENLAIRHFLNGIR
HHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHH
CTAHVIHAVGQN
HHHHHHHHHCCC
>Mature Secondary Structure 
ATLFKLLRQESISTQNKGMRECAALMRSLMEEVGVCTRLFETKGHPILYGELLTDKHAP
HHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHCCCCCC
TLLIYGHYDVQPPDPLSEWETPPFEPTVRDGRIFARGAGDNKGQIVAQLLGIKTYQEACG
EEEEEEECCCCCCCCCHHCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHC
ALPVNIKIVIEGEEEMGSVHLPDFVQSNKELLQADLVYTADGPSHESSSPLVLLGVRGIL
CCCEEEEEEEECHHHCCCCCCCHHHHCCHHHHHHHEEEECCCCCCCCCCCEEEEEECCEE
SFEIELQNAEFDNHSGNTGNIVPNPAWDLMELLQTMRSTDGRVLIEGFYDNIRPASAEEE
EEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHH
QLLATLPFEKDSVAQKIGYPHLDIDGKSYYRKLALEPTFNICGLKSGYLEDGIKTIIPSK
HEEEECCCCHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCHHHCCHHHHCCCC
ASVKIDARLVVDQDPKDIFEKVTAHVKARRPDAKVTFLGAMEPSRTPVETAIVQKALKGI
CCEEEEEEEEECCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHH
SACFNEEPLIQPSLGGSLPDYVWTKLLHAPSLLVPYANFDQRNHSPNENLAIRHFLNGIR
HHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHH
CTAHVIHAVGQN
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]