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The map label for this gene is pckA

Identifier: 56964643

GI number: 56964643

Start: 3008703

End: 3010286

Strand: Direct

Name: pckA

Synonym: ABC2879

Alternate gene names: 56964643

Gene position: 3008703-3010286 (Clockwise)

Preceding gene: 56964641

Following gene: 56964645

Centisome position: 69.91

GC content: 48.11

Gene sequence:

>1584_bases
ATGAAAATGCTTCACCGTTCAGCTTTACTAGAACAATTGCTTTCAAGCCCTAAAACGTTGCACCAATTGCCCGCTTCGAC
ACTCGTCGAGCATGCACTCCAACGAAATGAAGGCATATTGACCGATTCTGGCGCACTTGCTGTCAAAACAGGCCTTTACA
CAGGCCGCTCGCCTCAAGACAAGTTTATTGTCGATGAACCAATGTCTAGAGATTCCATCCATTGGGGCGCAACGAATAAG
CCCATTTCATCGGAAGTGTTCACCCGGCTAAAAGACAAAGTGCTACATTATTTGCAACAAAAAGAGGTCCGTTACGCATC
CGTTGGCTTCGCTGGCGCTGATCCTTCTTTCCAACTGCCGATTACGTATTTAAATGAGTTTGCTTGGCATCACTTGTTTG
CTAGACAGCTATTTATTCGTGAGCAAGCTCCTTCAAACAATGGTTTGGAACCATTTACGATCATTAGCGCACCGACGTTT
AAAGCCGATCCATCCGTCGACGGCACTCGCTCAGAAGCGTTTGTCATCATCTCATTTGAGCAACGCATTATTTTAATTGG
CGGTACGGAATATGCCGGTGAAATGAAAAAAGCGATCTTTTCCGTGATGAATTACTTGCTCCCTGAAGCAGGCGTGCTGC
CAATGCACTGCTCAGCCAACACAACCGATGAAGGAGAAACGGCACTCTTTTTTGGCCTCTCAGGTACCGGCAAAACGACA
CTGTCGGCTTCCGAACATAGGCGCCTAATCGGCGATGACGAGCACGGCTGGTCAGAAAAAGGCATCTTTAACATTGAAGG
AGGCTGCTACGCCAAATGCATCGGCCTTTCAAAAGAAAGCGAACCACAAATTTGGGACGCTATTGCTTTCGGGGCAGTCC
TTGAAAATGTCGTCGTCGATCCAAACACGGGCAAACCTGACTTTAAAAGCGGTGAGTTGACGGAAAATACGCGGGCTGCC
TACCCGCTTGAAGCGATTCCAAATGCTTTGCTGCCTAGTGTTGCCGGGCCTCCAAAAGCGATAATATTCTTGACTGCTGA
TGCATTTGGCGTATTGCCGCCCATTAGCAAGTTGACAAAAGAACAAGCAATGTACCATTTTCTATCTGGTTACACAAGCA
AACTAGCAGGAACGGAACGAGGCATCACGCAACCTGAGGCGACATTTTCTACTTGCTTTGGCGCTCCGTTTTTGCCACGC
AAACCAGAAGAGTACGCTACTATGCTTGGGGACAAATTGGAAAATGACGACGTCCAAGTGTTTCTTGTAAACACAGGCTG
GACAGGGGGTAGCTATGGAACTGGCACACGCATTAAGCTTGCTTATACACGTGCCATGGTAGAAGCGGCTTTACAAGGAA
AATTGGATGAGATTGAAACAGTGTGCGATCCCATTTTTGGCCTTTATGTCCCAACACACTGCCCAGGAGTCCCTGATGAG
CTGTTGACCCCTCGACGTGTTTGGAAGGACGAAGCAGCTTACGAGGCAACCGCCTTACAGCTTGCTAAGCAATTCCACGA
AAATTTTTCTGCCTTTCCGGCAGCAAAAGACAGCGTCCGTCTTGCTGGACCACGCCTTGCATAA

Upstream 100 bases:

>100_bases
ATCTAAAAAAGAATGACCTATTTATTCACTTTAGTATGGACTATTAAATAAAATGTGTTATACTAAAGCCACTTAAAATT
GCAAAAAGGAGCCATTAAAC

Downstream 100 bases:

>100_bases
CCTTTAACAAGCAGGCAGCTTTTTAGCTGCCTGCTTGATTTTGTCGACAAAGTCGATAAACCGCATCATACTGATAGAAA
ATGCTTGTCAGACGAGGAGT

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase

Number of amino acids: Translated: 527; Mature: 527

Protein sequence:

>527_residues
MKMLHRSALLEQLLSSPKTLHQLPASTLVEHALQRNEGILTDSGALAVKTGLYTGRSPQDKFIVDEPMSRDSIHWGATNK
PISSEVFTRLKDKVLHYLQQKEVRYASVGFAGADPSFQLPITYLNEFAWHHLFARQLFIREQAPSNNGLEPFTIISAPTF
KADPSVDGTRSEAFVIISFEQRIILIGGTEYAGEMKKAIFSVMNYLLPEAGVLPMHCSANTTDEGETALFFGLSGTGKTT
LSASEHRRLIGDDEHGWSEKGIFNIEGGCYAKCIGLSKESEPQIWDAIAFGAVLENVVVDPNTGKPDFKSGELTENTRAA
YPLEAIPNALLPSVAGPPKAIIFLTADAFGVLPPISKLTKEQAMYHFLSGYTSKLAGTERGITQPEATFSTCFGAPFLPR
KPEEYATMLGDKLENDDVQVFLVNTGWTGGSYGTGTRIKLAYTRAMVEAALQGKLDEIETVCDPIFGLYVPTHCPGVPDE
LLTPRRVWKDEAAYEATALQLAKQFHENFSAFPAAKDSVRLAGPRLA

Sequences:

>Translated_527_residues
MKMLHRSALLEQLLSSPKTLHQLPASTLVEHALQRNEGILTDSGALAVKTGLYTGRSPQDKFIVDEPMSRDSIHWGATNK
PISSEVFTRLKDKVLHYLQQKEVRYASVGFAGADPSFQLPITYLNEFAWHHLFARQLFIREQAPSNNGLEPFTIISAPTF
KADPSVDGTRSEAFVIISFEQRIILIGGTEYAGEMKKAIFSVMNYLLPEAGVLPMHCSANTTDEGETALFFGLSGTGKTT
LSASEHRRLIGDDEHGWSEKGIFNIEGGCYAKCIGLSKESEPQIWDAIAFGAVLENVVVDPNTGKPDFKSGELTENTRAA
YPLEAIPNALLPSVAGPPKAIIFLTADAFGVLPPISKLTKEQAMYHFLSGYTSKLAGTERGITQPEATFSTCFGAPFLPR
KPEEYATMLGDKLENDDVQVFLVNTGWTGGSYGTGTRIKLAYTRAMVEAALQGKLDEIETVCDPIFGLYVPTHCPGVPDE
LLTPRRVWKDEAAYEATALQLAKQFHENFSAFPAAKDSVRLAGPRLA
>Mature_527_residues
MKMLHRSALLEQLLSSPKTLHQLPASTLVEHALQRNEGILTDSGALAVKTGLYTGRSPQDKFIVDEPMSRDSIHWGATNK
PISSEVFTRLKDKVLHYLQQKEVRYASVGFAGADPSFQLPITYLNEFAWHHLFARQLFIREQAPSNNGLEPFTIISAPTF
KADPSVDGTRSEAFVIISFEQRIILIGGTEYAGEMKKAIFSVMNYLLPEAGVLPMHCSANTTDEGETALFFGLSGTGKTT
LSASEHRRLIGDDEHGWSEKGIFNIEGGCYAKCIGLSKESEPQIWDAIAFGAVLENVVVDPNTGKPDFKSGELTENTRAA
YPLEAIPNALLPSVAGPPKAIIFLTADAFGVLPPISKLTKEQAMYHFLSGYTSKLAGTERGITQPEATFSTCFGAPFLPR
KPEEYATMLGDKLENDDVQVFLVNTGWTGGSYGTGTRIKLAYTRAMVEAALQGKLDEIETVCDPIFGLYVPTHCPGVPDE
LLTPRRVWKDEAAYEATALQLAKQFHENFSAFPAAKDSVRLAGPRLA

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family

Homologues:

Organism=Escherichia coli, GI1789807, Length=502, Percent_Identity=48.207171314741, Blast_Score=456, Evalue=1e-129,
Organism=Saccharomyces cerevisiae, GI6322950, Length=511, Percent_Identity=48.53228962818, Blast_Score=472, Evalue=1e-134,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): PCKA_BACSK (Q5WDZ7)

Other databases:

- EMBL:   AP006627
- RefSeq:   YP_176374.1
- ProteinModelPortal:   Q5WDZ7
- SMR:   Q5WDZ7
- STRING:   Q5WDZ7
- EnsemblBacteria:   EBBACT00000047004
- GeneID:   3202085
- GenomeReviews:   AP006627_GR
- KEGG:   bcl:ABC2879
- NMPDR:   fig|66692.3.peg.2768
- eggNOG:   COG1866
- GeneTree:   EBGT00050000001183
- HOGENOM:   HBG293387
- OMA:   PMHASAN
- ProtClustDB:   PRK09344
- BioCyc:   BCLA66692:ABC2879-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- HAMAP:   MF_00453
- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994
- Gene3D:   G3DSA:3.90.228.20
- Gene3D:   G3DSA:3.40.449.10
- PIRSF:   PIRSF006294
- TIGRFAMs:   TIGR00224

Pfam domain/function: PF01293 PEPCK_ATP; SSF68923 PEP_carboxykinase_N

EC number: =4.1.1.49

Molecular weight: Translated: 57668; Mature: 57668

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMLHRSALLEQLLSSPKTLHQLPASTLVEHALQRNEGILTDSGALAVKTGLYTGRSPQD
CCHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHCCCEEECCCCEEEEECCCCCCCCCC
KFIVDEPMSRDSIHWGATNKPISSEVFTRLKDKVLHYLQQKEVRYASVGFAGADPSFQLP
CEEECCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCH
ITYLNEFAWHHLFARQLFIREQAPSNNGLEPFTIISAPTFKADPSVDGTRSEAFVIISFE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEEC
QRIILIGGTEYAGEMKKAIFSVMNYLLPEAGVLPMHCSANTTDEGETALFFGLSGTGKTT
CEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCEEEEEEECCCCCCE
LSASEHRRLIGDDEHGWSEKGIFNIEGGCYAKCIGLSKESEPQIWDAIAFGAVLENVVVD
ECHHHHHHCCCCCCCCCCCCCEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHEEC
PNTGKPDFKSGELTENTRAAYPLEAIPNALLPSVAGPPKAIIFLTADAFGVLPPISKLTK
CCCCCCCCCCCCCCCCCCCCCCHHHCCHHHCCCCCCCCCEEEEEECCCCCCCCCHHHHHH
EQAMYHFLSGYTSKLAGTERGITQPEATFSTCFGAPFLPRKPEEYATMLGDKLENDDVQV
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCEEE
FLVNTGWTGGSYGTGTRIKLAYTRAMVEAALQGKLDEIETVCDPIFGLYVPTHCPGVPDE
EEEECCCCCCCCCCCCEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCHH
LLTPRRVWKDEAAYEATALQLAKQFHENFSAFPAAKDSVRLAGPRLA
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCC
>Mature Secondary Structure
MKMLHRSALLEQLLSSPKTLHQLPASTLVEHALQRNEGILTDSGALAVKTGLYTGRSPQD
CCHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHCCCEEECCCCEEEEECCCCCCCCCC
KFIVDEPMSRDSIHWGATNKPISSEVFTRLKDKVLHYLQQKEVRYASVGFAGADPSFQLP
CEEECCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCH
ITYLNEFAWHHLFARQLFIREQAPSNNGLEPFTIISAPTFKADPSVDGTRSEAFVIISFE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEEC
QRIILIGGTEYAGEMKKAIFSVMNYLLPEAGVLPMHCSANTTDEGETALFFGLSGTGKTT
CEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCEEEEEEECCCCCCE
LSASEHRRLIGDDEHGWSEKGIFNIEGGCYAKCIGLSKESEPQIWDAIAFGAVLENVVVD
ECHHHHHHCCCCCCCCCCCCCEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHEEC
PNTGKPDFKSGELTENTRAAYPLEAIPNALLPSVAGPPKAIIFLTADAFGVLPPISKLTK
CCCCCCCCCCCCCCCCCCCCCCHHHCCHHHCCCCCCCCCEEEEEECCCCCCCCCHHHHHH
EQAMYHFLSGYTSKLAGTERGITQPEATFSTCFGAPFLPRKPEEYATMLGDKLENDDVQV
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCEEE
FLVNTGWTGGSYGTGTRIKLAYTRAMVEAALQGKLDEIETVCDPIFGLYVPTHCPGVPDE
EEEECCCCCCCCCCCCEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCHH
LLTPRRVWKDEAAYEATALQLAKQFHENFSAFPAAKDSVRLAGPRLA
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA