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The map label for this gene is cisA [H]

Identifier: 56964629

GI number: 56964629

Start: 2992454

End: 2993857

Strand: Direct

Name: cisA [H]

Synonym: ABC2865

Alternate gene names: 56964629

Gene position: 2992454-2993857 (Clockwise)

Preceding gene: 56964628

Following gene: 56964630

Centisome position: 69.53

GC content: 39.74

Gene sequence:

>1404_bases
ATGAGAACAGCGGTTTACATTAGAGTTAGTACAGAAGAGCAGGTAAATGAAGGTTACTCTATATCAGCTCAAAGAGAACG
ACTTCAAGCGTATATAACTTCTCAAAATTGGGATGCGGTTGGTTTTTATGTTGACGAAGGGGTTTCAGCCAAAAACACTG
AAAGACCACAGTTAAAGAGAATGCTTAAACACATAGAAGAAGGGTTTATTGATGTGGTATTGGTTTATCGACTTGACCGT
TTAACCCGTTCTGTTCTTGACCTTTATCAGTTACTTGACACATTCGAAAGAAATGGGTGTAAATTCAAAAGCGCTACCGA
AGTTTATGACACAACTACTGCTATGGGTCGCCTTTTCATAACACTCGTGGCTGCGTTGGCACAATGGGAACGAGAAAATC
TTGGCGAACGAGTAAGAATGGGAATGACACAACGAACAAGAGAGGGCCAATGGCATGGCAGCTCCCCAGCATATGGCTAC
AAATATGAAAACGGTATTGTGTCTATCGACGATGAAAAAGCAAACATCGTCCGTTGGATTTATATGTCCTACATCGAGGG
CATGAGCGACCGGAAGTTAGCTGTAAAATTAAATGAACAAGGTATACCTACACAAAAAGGTGGTGCATGGAGAGAAAGCA
CCATTAGAAGAATACTTCAAAATGAAACATATACAGGAAAGCTTATGTGGGGCGTGCGAGTTAATAAAGAAAATGCCTTT
TCTGTAGATGGTGCAATGCCAAAAATAATCGAACAGGGCATATTTGAAAAAGCTCAATCCATTAGAAAAGGCAGAGCTAG
AGTTCACCCAAGGCAGGCAACAAGTAAACATATCTTTTCAGGGGCTTTACGCTGCGCACGTTGCGGGGCAGCGATGAAGG
GACACAAAAAGACAGATAAAGGCAAAGAGTACCGCTCTTATCGTTGCATCAACCGTTACAATCATACCTGTGATATGCCA
ATGATTAGCCAAAAAATTTTAGAAAAAACTTTCATCACTTACTTTAAAGATGTTGAAATTGTTGGCAGTTACCAACCGCC
AAAAGAAAATGAAGAAAGCCTACTAAACGTCGAAAAGGAATTGGAACAGATTAAAAAACGCCGAAAAAAATGGCAATACG
CTTGGGCTAATGATCTAATGAGTGATGAAGAGTATACCGAACGCATTCGTGAAGAAGAGGCGAAAGAGGAAGAGTTACTC
AAAGTAATAGATAACAAACAAAAACAAACATCGGCTGAAGAAGTCAGCATCTTGTCGGCTTTTATTACTGAGCAATGGAA
TAAGCTTGATGTACTTGAGAAAAAAACTTTTATTCAAAACACCGTTGAATCAATTAGAGTCGACAAAGTTACTGCTCAGC
GGCAAAAAGATCGTGTGAAAATATTAGAAGTGAAGTTCAAGTGA

Upstream 100 bases:

>100_bases
TGATTCTCAGCCCCGAAAATCCGCATATGCGCCCCCAATTTTATGATGAAGATGAAATACGCATCCTCGGAAAAGTCGTG
TCTGCATCAAGGACGTACGA

Downstream 100 bases:

>100_bases
GGTTAAGTTATGTATGATGTAATTGTTATCGGAGGCGGTCCCTCTGGCTTAATGGCGTCTGTAGCCGCCGCTAAAAACGG
CGCTAAGGTATTGCTCTTGG

Product: site-specific recombinase for integration and excision

Products: NA

Alternate protein names: Stage IV sporulation protein CA [H]

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MRTAVYIRVSTEEQVNEGYSISAQRERLQAYITSQNWDAVGFYVDEGVSAKNTERPQLKRMLKHIEEGFIDVVLVYRLDR
LTRSVLDLYQLLDTFERNGCKFKSATEVYDTTTAMGRLFITLVAALAQWERENLGERVRMGMTQRTREGQWHGSSPAYGY
KYENGIVSIDDEKANIVRWIYMSYIEGMSDRKLAVKLNEQGIPTQKGGAWRESTIRRILQNETYTGKLMWGVRVNKENAF
SVDGAMPKIIEQGIFEKAQSIRKGRARVHPRQATSKHIFSGALRCARCGAAMKGHKKTDKGKEYRSYRCINRYNHTCDMP
MISQKILEKTFITYFKDVEIVGSYQPPKENEESLLNVEKELEQIKKRRKKWQYAWANDLMSDEEYTERIREEEAKEEELL
KVIDNKQKQTSAEEVSILSAFITEQWNKLDVLEKKTFIQNTVESIRVDKVTAQRQKDRVKILEVKFK

Sequences:

>Translated_467_residues
MRTAVYIRVSTEEQVNEGYSISAQRERLQAYITSQNWDAVGFYVDEGVSAKNTERPQLKRMLKHIEEGFIDVVLVYRLDR
LTRSVLDLYQLLDTFERNGCKFKSATEVYDTTTAMGRLFITLVAALAQWERENLGERVRMGMTQRTREGQWHGSSPAYGY
KYENGIVSIDDEKANIVRWIYMSYIEGMSDRKLAVKLNEQGIPTQKGGAWRESTIRRILQNETYTGKLMWGVRVNKENAF
SVDGAMPKIIEQGIFEKAQSIRKGRARVHPRQATSKHIFSGALRCARCGAAMKGHKKTDKGKEYRSYRCINRYNHTCDMP
MISQKILEKTFITYFKDVEIVGSYQPPKENEESLLNVEKELEQIKKRRKKWQYAWANDLMSDEEYTERIREEEAKEEELL
KVIDNKQKQTSAEEVSILSAFITEQWNKLDVLEKKTFIQNTVESIRVDKVTAQRQKDRVKILEVKFK
>Mature_467_residues
MRTAVYIRVSTEEQVNEGYSISAQRERLQAYITSQNWDAVGFYVDEGVSAKNTERPQLKRMLKHIEEGFIDVVLVYRLDR
LTRSVLDLYQLLDTFERNGCKFKSATEVYDTTTAMGRLFITLVAALAQWERENLGERVRMGMTQRTREGQWHGSSPAYGY
KYENGIVSIDDEKANIVRWIYMSYIEGMSDRKLAVKLNEQGIPTQKGGAWRESTIRRILQNETYTGKLMWGVRVNKENAF
SVDGAMPKIIEQGIFEKAQSIRKGRARVHPRQATSKHIFSGALRCARCGAAMKGHKKTDKGKEYRSYRCINRYNHTCDMP
MISQKILEKTFITYFKDVEIVGSYQPPKENEESLLNVEKELEQIKKRRKKWQYAWANDLMSDEEYTERIREEEAKEEELL
KVIDNKQKQTSAEEVSILSAFITEQWNKLDVLEKKTFIQNTVESIRVDKVTAQRQKDRVKILEVKFK

Specific function: Putative site-specific recombinase having a very important role in sporulation. It probably plays a role in the recombination of spoIIIC and spoIVCB to form sigma K factor [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 recombinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=151, Percent_Identity=33.7748344370861, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786756, Length=374, Percent_Identity=23.5294117647059, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011109
- InterPro:   IPR006118
- InterPro:   IPR006119 [H]

Pfam domain/function: PF07508 Recombinase; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 54481; Mature: 54481

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: PS00397 RECOMBINASES_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTAVYIRVSTEEQVNEGYSISAQRERLQAYITSQNWDAVGFYVDEGVSAKNTERPQLKR
CCEEEEEEECCHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCHHHHH
MLKHIEEGFIDVVLVYRLDRLTRSVLDLYQLLDTFERNGCKFKSATEVYDTTTAMGRLFI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
TLVAALAQWERENLGERVRMGMTQRTREGQWHGSSPAYGYKYENGIVSIDDEKANIVRWI
HHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCEEECCCEEEECCCCHHHHHHH
YMSYIEGMSDRKLAVKLNEQGIPTQKGGAWRESTIRRILQNETYTGKLMWGVRVNKENAF
HHHHHCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEECCCCCE
SVDGAMPKIIEQGIFEKAQSIRKGRARVHPRQATSKHIFSGALRCARCGAAMKGHKKTDK
ECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GKEYRSYRCINRYNHTCDMPMISQKILEKTFITYFKDVEIVGSYQPPKENEESLLNVEKE
CHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHH
LEQIKKRRKKWQYAWANDLMSDEEYTERIREEEAKEEELLKVIDNKQKQTSAEEVSILSA
HHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
FITEQWNKLDVLEKKTFIQNTVESIRVDKVTAQRQKDRVKILEVKFK
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECC
>Mature Secondary Structure
MRTAVYIRVSTEEQVNEGYSISAQRERLQAYITSQNWDAVGFYVDEGVSAKNTERPQLKR
CCEEEEEEECCHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCHHHHH
MLKHIEEGFIDVVLVYRLDRLTRSVLDLYQLLDTFERNGCKFKSATEVYDTTTAMGRLFI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
TLVAALAQWERENLGERVRMGMTQRTREGQWHGSSPAYGYKYENGIVSIDDEKANIVRWI
HHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCEEECCCEEEECCCCHHHHHHH
YMSYIEGMSDRKLAVKLNEQGIPTQKGGAWRESTIRRILQNETYTGKLMWGVRVNKENAF
HHHHHCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEECCCCCE
SVDGAMPKIIEQGIFEKAQSIRKGRARVHPRQATSKHIFSGALRCARCGAAMKGHKKTDK
ECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GKEYRSYRCINRYNHTCDMPMISQKILEKTFITYFKDVEIVGSYQPPKENEESLLNVEKE
CHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHH
LEQIKKRRKKWQYAWANDLMSDEEYTERIREEEAKEEELLKVIDNKQKQTSAEEVSILSA
HHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
FITEQWNKLDVLEKKTFIQNTVESIRVDKVTAQRQKDRVKILEVKFK
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2105293; 7704261; 8969508; 9384377 [H]