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The map label for this gene is def

Identifier: 56964188

GI number: 56964188

Start: 2551948

End: 2552499

Strand: Direct

Name: def

Synonym: ABC2423

Alternate gene names: 56964188

Gene position: 2551948-2552499 (Clockwise)

Preceding gene: 56964187

Following gene: 56964189

Centisome position: 59.29

GC content: 47.46

Gene sequence:

>552_bases
ATGATTACGATGGACGACATTGTTCGCGAAGGCAATCCAATTTTACGTAAAGTCGCTGAGCCGGTCGAATTGCCATTAAC
AGATGAAGACAAGCAAACATTGGTCGACATGCTCGAGTTTATTAAAAACAGCCAAGACCCTGAAATCGCGGAAAAATACG
GGCTCCGTCCTGGCGTAGGCTTAGCTGCGCCACAAATCGGCATTTCTAAACGGCTATTTGCCATCCATGCCACAGATGAA
AACGGCAACCTCTACAGCATGGGAATCGCCAATCCGAAAGTCGTTAGCCATTCTGTTGAAACCAATGAACTTGAAAATGG
CGAAGGTTGTCTCTCTGTTGACCGTGACGTTCCCGGCCTTGTGCCTCGGCGTGCCCGTTTAACGATCACTGGAGTCGACC
ATGAAGGCAACGACGTGCGTATGCGCTTGCGGGGATATATTGCCATTGTTTTTCAACATGAGTTGGATCATTTAGACGGC
ATTATGTTTTACGACCGAATTGAGGGACTCGAAGACCCTTTCAAAAAACCATTGCCTGGATTTGCGCTTTAA

Upstream 100 bases:

>100_bases
AAGCCATATGTGCGAGCAGCCTGTCGTCTGCTCGCTTTTTCTACTTGCGCCTGCTACTGAATATCCTTAAACTATATAGA
GAAAATAAGGAGTGGTACAT

Downstream 100 bases:

>100_bases
CAGCTGCCAACAATTCCCTCGATTCTTCAATGCCTACGTGCATACACTACATGTACAAGGGCATTGAAAAGGAGTGGAAC
GGGATGTTAAGAAGGTGGCG

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 183; Mature: 183

Protein sequence:

>183_residues
MITMDDIVREGNPILRKVAEPVELPLTDEDKQTLVDMLEFIKNSQDPEIAEKYGLRPGVGLAAPQIGISKRLFAIHATDE
NGNLYSMGIANPKVVSHSVETNELENGEGCLSVDRDVPGLVPRRARLTITGVDHEGNDVRMRLRGYIAIVFQHELDHLDG
IMFYDRIEGLEDPFKKPLPGFAL

Sequences:

>Translated_183_residues
MITMDDIVREGNPILRKVAEPVELPLTDEDKQTLVDMLEFIKNSQDPEIAEKYGLRPGVGLAAPQIGISKRLFAIHATDE
NGNLYSMGIANPKVVSHSVETNELENGEGCLSVDRDVPGLVPRRARLTITGVDHEGNDVRMRLRGYIAIVFQHELDHLDG
IMFYDRIEGLEDPFKKPLPGFAL
>Mature_183_residues
MITMDDIVREGNPILRKVAEPVELPLTDEDKQTLVDMLEFIKNSQDPEIAEKYGLRPGVGLAAPQIGISKRLFAIHATDE
NGNLYSMGIANPKVVSHSVETNELENGEGCLSVDRDVPGLVPRRARLTITGVDHEGNDVRMRLRGYIAIVFQHELDHLDG
IMFYDRIEGLEDPFKKPLPGFAL

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Escherichia coli, GI1789682, Length=157, Percent_Identity=32.484076433121, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_BACSK (Q5WFA2)

Other databases:

- EMBL:   AP006627
- RefSeq:   YP_175919.1
- HSSP:   O31410
- ProteinModelPortal:   Q5WFA2
- SMR:   Q5WFA2
- STRING:   Q5WFA2
- EnsemblBacteria:   EBBACT00000047952
- GeneID:   3201620
- GenomeReviews:   AP006627_GR
- KEGG:   bcl:ABC2423
- NMPDR:   fig|66692.3.peg.2110
- eggNOG:   COG0242
- GeneTree:   EBGT00050000001175
- HOGENOM:   HBG665227
- OMA:   DIIKEGH
- ProtClustDB:   PRK00150
- BioCyc:   BCLA66692:ABC2423-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 20389; Mature: 20389

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: NA

Important sites: ACT_SITE 154-154

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITMDDIVREGNPILRKVAEPVELPLTDEDKQTLVDMLEFIKNSQDPEIAEKYGLRPGVG
CCCHHHHHHCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCC
LAAPQIGISKRLFAIHATDENGNLYSMGIANPKVVSHSVETNELENGEGCLSVDRDVPGL
CCCCCCCCCCEEEEEEEECCCCCEEEECCCCCHHEECCCCCCCCCCCCCCEEECCCCCCC
VPRRARLTITGVDHEGNDVRMRLRGYIAIVFQHELDHLDGIMFYDRIEGLEDPFKKPLPG
CCCCEEEEEEECCCCCCCEEEEEEEEEEEEEECCHHHHCCCHHHHHHCCHHHHHHCCCCC
FAL
CCC
>Mature Secondary Structure
MITMDDIVREGNPILRKVAEPVELPLTDEDKQTLVDMLEFIKNSQDPEIAEKYGLRPGVG
CCCHHHHHHCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCC
LAAPQIGISKRLFAIHATDENGNLYSMGIANPKVVSHSVETNELENGEGCLSVDRDVPGL
CCCCCCCCCCEEEEEEEECCCCCEEEECCCCCHHEECCCCCCCCCCCCCCEEECCCCCCC
VPRRARLTITGVDHEGNDVRMRLRGYIAIVFQHELDHLDGIMFYDRIEGLEDPFKKPLPG
CCCCEEEEEEECCCCCCCEEEEEEEEEEEEEECCHHHHCCCHHHHHHCCHHHHHHCCCCC
FAL
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA