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The map label for this gene is opuD [H]

Identifier: 56964175

GI number: 56964175

Start: 2537333

End: 2538979

Strand: Direct

Name: opuD [H]

Synonym: ABC2410

Alternate gene names: 56964175

Gene position: 2537333-2538979 (Clockwise)

Preceding gene: 56964172

Following gene: 56964176

Centisome position: 58.95

GC content: 46.27

Gene sequence:

>1647_bases
ATGAAACAAACAACTTCAGTATTTGGAACGGCTGTTGCAATTGCAAGCGCCTTTATCCTTTGGGGAGTATTTTTCCCCAA
AAATATGGCGACCGTAATGGACTCCATCCAAGGCTTTATCTCATCTGAACTTGGTTGGTTCTATTTGCTCTCTGCCACTT
TTTATGTAGGCGTGGGCATTTACTTAATTTTTAGCCCTTATGGAAGTATTAAACTCGGTAAACCTGAAGAAGAACCTGAA
TACAGCTATATGACATGGTTTGCGTTTCTTTTTACAGCCGGCATGGGCATCGGCCTTGTTTTTTGGGGAGCGGCTGAACC
ACTGTCCCATTATTTTGCTCCTCCAGATGCTGCACCAGGCACGCAAGATGCAGCTGCCCAGGCGATGCGGTATGCGATCT
TTCATTGGGGCATCCATCCATGGGCCATCTATGCTGTTGTGGCGCTGGCACTCGCCTATTTTCAGTACCGTAAAGACGAG
CCTGCTTTATTTAGCTCGACATTCCGGCCGCTCATTGGCCACCGCATTACAGGGCCGCTTGGCAAGGTCATTGACGTCTT
TGTCGTGTTCGCGACCGTTTTTGGAACGGCGACATCACTTGGTTTCGGAGCCGCGCAAATTACAGCCGGTCTTCGCTATT
TGTTTCCTGCATTAAATTCGTTTTCCGATAATGTATTTCAGTTGGTTGTCATTGCCATTGTAACAATTCTCTTTTGTATT
TCGGCGGCAACCGGCGTCGACAAAGGCATTCGCATATTGAGTAATTTTAATGTACGACTTGCTATTATTTTAATGGCCTT
TGTGTTGCTTCTCGGTCCGACCAGTTACATAATGGGCGTTTTTACACAAGGCATTGGCAGCTATGCCCAAAACTTTTTTG
GCATGAGTTTTAGCATGGATGTTTATAACCCAGAATCTTCTTGGGTGCAAGAATGGACATTGTTTTACTGGGCATGGTGG
ATTTCGTGGTCCCCTTTCGTCGGGGCATTTATTGCCCGTGTTTCACGGGGGCGCACAATTCGCGAGTTTGTCATTGGCGT
TATCGCACTGCCGTCTTTGTTCGGCGCTTTTTGGTTTAGCGTATTTGGCGGCTCTAGCATCTTTTTTGAAAACGAAACAG
GTGCCCTTTATGGGCAAATGCAAACAGGCGGCACGGAAATGGCATTGTTTGCCTTGCTTGAGCAATTCCCATTGACCTTT
TTCGTTTCCATTCTGACCGTCATTCTAATCGCCTCCTTTTTTGTAACATCAGCCGACTCGGCAACGGTCGTGCTCGGTAT
GCAAACAACTGGTGGCCTTCTAAATCCGCCTAATGCGGTAAAGTTGGTGTGGGGCGTTATTATTTCCGGAACAGCTGCTG
TACTACTGCTAACAAGCGAACAAGGTTTGGCCGCGCTGCAAACGGCATCGATTATTGGTGCTTTTCCGTTTGCTGTGATT
ATCATTTTAATGATTGTGTCGCTTTTTAAAGAACTCCGCAATGAAAAAGCAGCACTGACAGTAAACCGTGAACGGCTCCG
GCAAGAGCGCCTAGCTTTGCGCAATGAACGGGAACGCATTAAACGGGAACAAATGTTGCTTCGCAAAGAACAACAAAAAC
TTCGGCAACTAACAAAAGACAAGAAGAAGGACAGAAAAAAAGACTAA

Upstream 100 bases:

>100_bases
GCGATTGCGATGAAAATTGGCGTATCTTCCCTTGGATTGTTTGCCATCCTATGGTATGATGACGACTTGTGGACAACAAG
GAAAGGTTGAGATTGTACAA

Downstream 100 bases:

>100_bases
CTTGGTGTGCTCCTAGATGCAATCGATCTAGGAGCTTTTTTTGCGATATTTACAATCGATTCACAGTCGTTTTACATTTT
TATGACGCTAGAAAAAGACT

Product: choline/glycine betaine transporter

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 548; Mature: 548

Protein sequence:

>548_residues
MKQTTSVFGTAVAIASAFILWGVFFPKNMATVMDSIQGFISSELGWFYLLSATFYVGVGIYLIFSPYGSIKLGKPEEEPE
YSYMTWFAFLFTAGMGIGLVFWGAAEPLSHYFAPPDAAPGTQDAAAQAMRYAIFHWGIHPWAIYAVVALALAYFQYRKDE
PALFSSTFRPLIGHRITGPLGKVIDVFVVFATVFGTATSLGFGAAQITAGLRYLFPALNSFSDNVFQLVVIAIVTILFCI
SAATGVDKGIRILSNFNVRLAIILMAFVLLLGPTSYIMGVFTQGIGSYAQNFFGMSFSMDVYNPESSWVQEWTLFYWAWW
ISWSPFVGAFIARVSRGRTIREFVIGVIALPSLFGAFWFSVFGGSSIFFENETGALYGQMQTGGTEMALFALLEQFPLTF
FVSILTVILIASFFVTSADSATVVLGMQTTGGLLNPPNAVKLVWGVIISGTAAVLLLTSEQGLAALQTASIIGAFPFAVI
IILMIVSLFKELRNEKAALTVNRERLRQERLALRNERERIKREQMLLRKEQQKLRQLTKDKKKDRKKD

Sequences:

>Translated_548_residues
MKQTTSVFGTAVAIASAFILWGVFFPKNMATVMDSIQGFISSELGWFYLLSATFYVGVGIYLIFSPYGSIKLGKPEEEPE
YSYMTWFAFLFTAGMGIGLVFWGAAEPLSHYFAPPDAAPGTQDAAAQAMRYAIFHWGIHPWAIYAVVALALAYFQYRKDE
PALFSSTFRPLIGHRITGPLGKVIDVFVVFATVFGTATSLGFGAAQITAGLRYLFPALNSFSDNVFQLVVIAIVTILFCI
SAATGVDKGIRILSNFNVRLAIILMAFVLLLGPTSYIMGVFTQGIGSYAQNFFGMSFSMDVYNPESSWVQEWTLFYWAWW
ISWSPFVGAFIARVSRGRTIREFVIGVIALPSLFGAFWFSVFGGSSIFFENETGALYGQMQTGGTEMALFALLEQFPLTF
FVSILTVILIASFFVTSADSATVVLGMQTTGGLLNPPNAVKLVWGVIISGTAAVLLLTSEQGLAALQTASIIGAFPFAVI
IILMIVSLFKELRNEKAALTVNRERLRQERLALRNERERIKREQMLLRKEQQKLRQLTKDKKKDRKKD
>Mature_548_residues
MKQTTSVFGTAVAIASAFILWGVFFPKNMATVMDSIQGFISSELGWFYLLSATFYVGVGIYLIFSPYGSIKLGKPEEEPE
YSYMTWFAFLFTAGMGIGLVFWGAAEPLSHYFAPPDAAPGTQDAAAQAMRYAIFHWGIHPWAIYAVVALALAYFQYRKDE
PALFSSTFRPLIGHRITGPLGKVIDVFVVFATVFGTATSLGFGAAQITAGLRYLFPALNSFSDNVFQLVVIAIVTILFCI
SAATGVDKGIRILSNFNVRLAIILMAFVLLLGPTSYIMGVFTQGIGSYAQNFFGMSFSMDVYNPESSWVQEWTLFYWAWW
ISWSPFVGAFIARVSRGRTIREFVIGVIALPSLFGAFWFSVFGGSSIFFENETGALYGQMQTGGTEMALFALLEQFPLTF
FVSILTVILIASFFVTSADSATVVLGMQTTGGLLNPPNAVKLVWGVIISGTAAVLLLTSEQGLAALQTASIIGAFPFAVI
IILMIVSLFKELRNEKAALTVNRERLRQERLALRNERERIKREQMLLRKEQQKLRQLTKDKKKDRKKD

Specific function: High-affinity uptake of glycine betaine [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=524, Percent_Identity=37.5954198473282, Blast_Score=357, Evalue=1e-100,
Organism=Escherichia coli, GI1788102, Length=458, Percent_Identity=32.9694323144105, Blast_Score=214, Evalue=2e-56,
Organism=Escherichia coli, GI1786224, Length=477, Percent_Identity=28.0922431865828, Blast_Score=186, Evalue=3e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 60674; Mature: 60674

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHCCCCC
>Mature Secondary Structure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HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8752321; 9387221; 9384377 [H]