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The map label for this gene is lcfA [H]

Identifier: 56963714

GI number: 56963714

Start: 2065426

End: 2066664

Strand: Direct

Name: lcfA [H]

Synonym: ABC1949

Alternate gene names: 56963714

Gene position: 2065426-2066664 (Clockwise)

Preceding gene: 56963713

Following gene: 56963715

Centisome position: 47.99

GC content: 48.51

Gene sequence:

>1239_bases
ATGTTAACTGTAAACGATTGCACCTATACAAAAGATGATTTGAAGCGGTGCGAACAACAGTTTGCTATGCACTCCATCTG
GAAAACAGCTGAACAATGGCGGATTGCTGTCTGCCCACAAGAAATTGAAAAATGGATTGCGCTCCTGTTATACGTCAAAA
AAAGAGGCGGCAGTATAGTGCCGATCCATCCAAGTACGCCTCTTGCTGCAGCGAAGAAGATTGCTGTGCAAGCTCGTTGT
GCTTACTTATTCTTTCATACGTTTGAAACGCCATTGACGCTCTCAAACCCGGTCAGCACCTTTCCTCCAGGGCTTGTCCA
GTTTAGTTCAGGCACGACCGGGGTACCAAAACAATTGGAGCGCAGTTGGTCATCGATTGATGAGGAGCTAGCCGCTTACA
CCGAGCGTATGGCTCGTGTGGACGTCGATACGGTCGTGGTTGCCTGTCCAGTCACCCATTCCTATGGACTCATTAGTGGG
GTGTTAGCCGGACTGTCCCAGGATAAGGAGGTAGTTGTGTTAACTGCGCAAAATCCAAAATATGTACTTAAAAAACTAAC
CGATTATCCCAACCATTTGTTGTATGGTGCCCCGCCACTTCTGTACGCATTGGCAAAGCTAAGCAATGGGGACACGCTTC
AGGCCGTCATGACCTCGGGAACACGTTTGCCAGAGGAATGGTTTCAGTCAATCCAATCAAACAGCCAAATGCTGCTGCAA
CAATATGGTTGCTCGGAAGCTGGCTGTATTAGCATCGCTATGCCACTTCGTTTTTCTGAAGAATTGGGCGTGCCCCTTCG
CCATGTACAGGCCAAAGCAGGGACGGCGAGAAACAATCCTAGCGAGATTCAAGTCACCATTGGCAGACGCACAATCAACA
CAGGCGATCTAGGCTACATCGATCAAGATGGAGCTCTTTGCTTTCGGGAACGACTGGACGACATGATTAATGTCGCGGGA
TTGAATGTTTATCCTCACGAAGTCGAACACGTATTGCGCGAACATCCTAGCGTAGAAGATGCGGTCGTCTATAAAAAGGC
AGATTCCTATGCAGGAGAGCGTGTTTGCGCGCAAGTGTCTGCCAGAACGGAGACGTTATCCCTTCCTGAATTGCGGAGCT
ATTGCATAAGCCGGCTAGCGCCTCATCAAGTTCCACATGAAATGACCATTGTTGAAGAGATTGCAAAAAGCGCGAACGGA
AAAGTAAGCCGCGTAAAGTTAGGAGAAGCAAAAGGATGA

Upstream 100 bases:

>100_bases
CTCCTGTTATCGCTACAGAGTCACTGCTAACGAGCAAGGTGACAGGGAAGGAGAACTGTCGCCATTTGGTCAACAATGAA
CTTGGGAGGGAAAGCCGTAC

Downstream 100 bases:

>100_bases
CGAAAAAGGAAGCTATGGATGCGATTTACACGATTTTAGAGCATGAATTAAAATTGCCAACGCTTGAACAGTTTCATCAA
GACGCGCGAATGAACGAAGA

Product: acyl-CoA synthetase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MLTVNDCTYTKDDLKRCEQQFAMHSIWKTAEQWRIAVCPQEIEKWIALLLYVKKRGGSIVPIHPSTPLAAAKKIAVQARC
AYLFFHTFETPLTLSNPVSTFPPGLVQFSSGTTGVPKQLERSWSSIDEELAAYTERMARVDVDTVVVACPVTHSYGLISG
VLAGLSQDKEVVVLTAQNPKYVLKKLTDYPNHLLYGAPPLLYALAKLSNGDTLQAVMTSGTRLPEEWFQSIQSNSQMLLQ
QYGCSEAGCISIAMPLRFSEELGVPLRHVQAKAGTARNNPSEIQVTIGRRTINTGDLGYIDQDGALCFRERLDDMINVAG
LNVYPHEVEHVLREHPSVEDAVVYKKADSYAGERVCAQVSARTETLSLPELRSYCISRLAPHQVPHEMTIVEEIAKSANG
KVSRVKLGEAKG

Sequences:

>Translated_412_residues
MLTVNDCTYTKDDLKRCEQQFAMHSIWKTAEQWRIAVCPQEIEKWIALLLYVKKRGGSIVPIHPSTPLAAAKKIAVQARC
AYLFFHTFETPLTLSNPVSTFPPGLVQFSSGTTGVPKQLERSWSSIDEELAAYTERMARVDVDTVVVACPVTHSYGLISG
VLAGLSQDKEVVVLTAQNPKYVLKKLTDYPNHLLYGAPPLLYALAKLSNGDTLQAVMTSGTRLPEEWFQSIQSNSQMLLQ
QYGCSEAGCISIAMPLRFSEELGVPLRHVQAKAGTARNNPSEIQVTIGRRTINTGDLGYIDQDGALCFRERLDDMINVAG
LNVYPHEVEHVLREHPSVEDAVVYKKADSYAGERVCAQVSARTETLSLPELRSYCISRLAPHQVPHEMTIVEEIAKSANG
KVSRVKLGEAKG
>Mature_412_residues
MLTVNDCTYTKDDLKRCEQQFAMHSIWKTAEQWRIAVCPQEIEKWIALLLYVKKRGGSIVPIHPSTPLAAAKKIAVQARC
AYLFFHTFETPLTLSNPVSTFPPGLVQFSSGTTGVPKQLERSWSSIDEELAAYTERMARVDVDTVVVACPVTHSYGLISG
VLAGLSQDKEVVVLTAQNPKYVLKKLTDYPNHLLYGAPPLLYALAKLSNGDTLQAVMTSGTRLPEEWFQSIQSNSQMLLQ
QYGCSEAGCISIAMPLRFSEELGVPLRHVQAKAGTARNNPSEIQVTIGRRTINTGDLGYIDQDGALCFRERLDDMINVAG
LNVYPHEVEHVLREHPSVEDAVVYKKADSYAGERVCAQVSARTETLSLPELRSYCISRLAPHQVPHEMTIVEEIAKSANG
KVSRVKLGEAKG

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI58082049, Length=354, Percent_Identity=27.1186440677966, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI157311624, Length=354, Percent_Identity=26.5536723163842, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI157311622, Length=354, Percent_Identity=26.5536723163842, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI115511026, Length=121, Percent_Identity=37.1900826446281, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI42544132, Length=136, Percent_Identity=33.8235294117647, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI122937307, Length=134, Percent_Identity=35.8208955223881, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI38505220, Length=350, Percent_Identity=25.1428571428571, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1788107, Length=353, Percent_Identity=27.1954674220963, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI145693145, Length=339, Percent_Identity=26.2536873156342, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI221142682, Length=117, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17559526, Length=368, Percent_Identity=27.445652173913, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17538037, Length=355, Percent_Identity=23.3802816901408, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17557194, Length=357, Percent_Identity=23.249299719888, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI25141359, Length=421, Percent_Identity=25.1781472684086, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI32563687, Length=339, Percent_Identity=20.9439528023599, Blast_Score=72, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17558820, Length=329, Percent_Identity=24.0121580547112, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319699, Length=341, Percent_Identity=22.8739002932551, Blast_Score=80, Evalue=6e-16,
Organism=Drosophila melanogaster, GI18859661, Length=348, Percent_Identity=27.5862068965517, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24581924, Length=347, Percent_Identity=23.9193083573487, Blast_Score=85, Evalue=8e-17,
Organism=Drosophila melanogaster, GI281365686, Length=149, Percent_Identity=30.8724832214765, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24648257, Length=115, Percent_Identity=34.7826086956522, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 45546; Mature: 45546

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTVNDCTYTKDDLKRCEQQFAMHSIWKTAEQWRIAVCPQEIEKWIALLLYVKKRGGSIV
CEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCEEEECHHHHHHHHHHHHHHHHCCCEEE
PIHPSTPLAAAKKIAVQARCAYLFFHTFETPLTLSNPVSTFPPGLVQFSSGTTGVPKQLE
EECCCCCHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCEEECCCCCCCCHHHH
RSWSSIDEELAAYTERMARVDVDTVVVACPVTHSYGLISGVLAGLSQDKEVVVLTAQNPK
HHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCCCEEEEEEECCHH
YVLKKLTDYPNHLLYGAPPLLYALAKLSNGDTLQAVMTSGTRLPEEWFQSIQSNSQMLLQ
HHHHHHHCCCCCEECCCCHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHCCHHHHHH
QYGCSEAGCISIAMPLRFSEELGVPLRHVQAKAGTARNNPSEIQVTIGRRTINTGDLGYI
HHCCCCCCEEEEEECCCCCHHHCCCHHHHHHHCCCCCCCCCEEEEEECCEEECCCCCCEE
DQDGALCFRERLDDMINVAGLNVYPHEVEHVLREHPSVEDAVVYKKADSYAGERVCAQVS
CCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHEEECCHHHHHHHHHHHHH
ARTETLSLPELRSYCISRLAPHQVPHEMTIVEEIAKSANGKVSRVKLGEAKG
HCCHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCC
>Mature Secondary Structure
MLTVNDCTYTKDDLKRCEQQFAMHSIWKTAEQWRIAVCPQEIEKWIALLLYVKKRGGSIV
CEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCEEEECHHHHHHHHHHHHHHHHCCCEEE
PIHPSTPLAAAKKIAVQARCAYLFFHTFETPLTLSNPVSTFPPGLVQFSSGTTGVPKQLE
EECCCCCHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCEEECCCCCCCCHHHH
RSWSSIDEELAAYTERMARVDVDTVVVACPVTHSYGLISGVLAGLSQDKEVVVLTAQNPK
HHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCCCEEEEEEECCHH
YVLKKLTDYPNHLLYGAPPLLYALAKLSNGDTLQAVMTSGTRLPEEWFQSIQSNSQMLLQ
HHHHHHHCCCCCEECCCCHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHCCHHHHHH
QYGCSEAGCISIAMPLRFSEELGVPLRHVQAKAGTARNNPSEIQVTIGRRTINTGDLGYI
HHCCCCCCEEEEEECCCCCHHHCCCHHHHHHHCCCCCCCCCEEEEEECCEEECCCCCCEE
DQDGALCFRERLDDMINVAGLNVYPHEVEHVLREHPSVEDAVVYKKADSYAGERVCAQVS
CCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHEEECCHHHHHHHHHHHHH
ARTETLSLPELRSYCISRLAPHQVPHEMTIVEEIAKSANGKVSRVKLGEAKG
HCCHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]