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The map label for this gene is ansZ [H]

Identifier: 56963629

GI number: 56963629

Start: 1981103

End: 1982068

Strand: Direct

Name: ansZ [H]

Synonym: ABC1864

Alternate gene names: 56963629

Gene position: 1981103-1982068 (Clockwise)

Preceding gene: 56963628

Following gene: 56963630

Centisome position: 46.03

GC content: 46.89

Gene sequence:

>966_bases
ATGAATATGCGCCATGTGATGTTGCTGACAACTGGAGGCACGATTGCAAGCACTACAAGCGAAAGCGGCAAATTGGTTGC
TGGTGAACTTTCTGGAGAAGAGTTGGCAAAGCTATGTGGTTTGCCGGAAGACATCCATGTAAGCGTGCGCTCTGTATTGC
AAAAGCCAAGCGTTCATATGACACTCGCCGATTGGCTGTTGTTAAAGCATGAAGTAGAGCGTGCTTTTGCAGACGAGACG
ATTAACGGGATTGTCGTCACACACGGCACAGATACGCTTGAAGAAACAGCTTATTTTTTAGACTTAACGAACAAAGACGA
GCGTCCCATAATCGTCACAGGTTCTCAACGGGGACCAGAGCAAACAGGCAGTGATGCGTTTATGAATTTGCGTCATGCCA
TTTATGCTGCTTGTGAACCTGATTTAAAGCACACTGGCTGTGTCGTCGTGTTTAACGAGCGGATTTTTGCAGCCCGTTAT
GTCAAAAAAGAGCATGCCTCCAATTTGCAAGGGTTCAATGCCTTTGGTTTCGGCTATTTAGGGATTATAGACAACGATGT
GATTTTTACGTATCAAAAGCCTGTTCGCCGGGAGTTTTATGAAATTGGCAATACGCCTCTTAAACAAATTGGCATTGCTA
AAGTTTATTTAGGCATGAGCGCAGACATGCTGGAAGGGATGATTGCTGTATGCGACGGGATGGTGGTTGAAGGCGTTGGC
CGCGGCCAAGTTCCTCCAAACCTAGTAGCTACGTTGGAACGAGCGGTTGCCAACAAGAAGCCAATCATTGTGACGACCGC
CTCAGAAGAAGGGAAAGTATACCCAACGTATGACTACAAAGGAAGTGCCCACCAACTCGAACAAATGGGCGTTATTCTCG
GCTCCGATTATGACAGCAAGAAAGCACGGATTAAAGCAATGGTCATGCTGGCAGCGAACAAGGAACTAGCAGCTGGGTTT
ACGTGA

Upstream 100 bases:

>100_bases
TTGCTGCTGGAAACAATGCTAATGAAATTTTTATTGAAAATGGTCGTCTCCACGGCCCTTTGATTGCACAAGCAATTGCG
CGCAGGATCGAAATGGAGTG

Downstream 100 bases:

>100_bases
TGGCGCTTGCTGATCGGAACAAGCCGGTTTTATACTAGAAAAAAGGAATGTTCTATTAGCGAACCAAAGAGAGTAGGCGG
TCAGCCATGGTATCCCTTGT

Product: L-asparaginase

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MNMRHVMLLTTGGTIASTTSESGKLVAGELSGEELAKLCGLPEDIHVSVRSVLQKPSVHMTLADWLLLKHEVERAFADET
INGIVVTHGTDTLEETAYFLDLTNKDERPIIVTGSQRGPEQTGSDAFMNLRHAIYAACEPDLKHTGCVVVFNERIFAARY
VKKEHASNLQGFNAFGFGYLGIIDNDVIFTYQKPVRREFYEIGNTPLKQIGIAKVYLGMSADMLEGMIAVCDGMVVEGVG
RGQVPPNLVATLERAVANKKPIIVTTASEEGKVYPTYDYKGSAHQLEQMGVILGSDYDSKKARIKAMVMLAANKELAAGF
T

Sequences:

>Translated_321_residues
MNMRHVMLLTTGGTIASTTSESGKLVAGELSGEELAKLCGLPEDIHVSVRSVLQKPSVHMTLADWLLLKHEVERAFADET
INGIVVTHGTDTLEETAYFLDLTNKDERPIIVTGSQRGPEQTGSDAFMNLRHAIYAACEPDLKHTGCVVVFNERIFAARY
VKKEHASNLQGFNAFGFGYLGIIDNDVIFTYQKPVRREFYEIGNTPLKQIGIAKVYLGMSADMLEGMIAVCDGMVVEGVG
RGQVPPNLVATLERAVANKKPIIVTTASEEGKVYPTYDYKGSAHQLEQMGVILGSDYDSKKARIKAMVMLAANKELAAGF
T
>Mature_321_residues
MNMRHVMLLTTGGTIASTTSESGKLVAGELSGEELAKLCGLPEDIHVSVRSVLQKPSVHMTLADWLLLKHEVERAFADET
INGIVVTHGTDTLEETAYFLDLTNKDERPIIVTGSQRGPEQTGSDAFMNLRHAIYAACEPDLKHTGCVVVFNERIFAARY
VKKEHASNLQGFNAFGFGYLGIIDNDVIFTYQKPVRREFYEIGNTPLKQIGIAKVYLGMSADMLEGMIAVCDGMVVEGVG
RGQVPPNLVATLERAVANKKPIIVTTASEEGKVYPTYDYKGSAHQLEQMGVILGSDYDSKKARIKAMVMLAANKELAAGF
T

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=334, Percent_Identity=27.2455089820359, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI1789327, Length=298, Percent_Identity=27.5167785234899, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1788065, Length=327, Percent_Identity=27.217125382263, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17558280, Length=266, Percent_Identity=24.0601503759398, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI193207372, Length=266, Percent_Identity=24.0601503759398, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17558278, Length=266, Percent_Identity=24.0601503759398, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI193207374, Length=266, Percent_Identity=24.0601503759398, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323189, Length=295, Percent_Identity=28.8135593220339, Blast_Score=112, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6323187, Length=295, Percent_Identity=28.8135593220339, Blast_Score=112, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6323186, Length=295, Percent_Identity=28.8135593220339, Blast_Score=112, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6323184, Length=295, Percent_Identity=28.8135593220339, Blast_Score=112, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6320527, Length=334, Percent_Identity=27.2455089820359, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24639609, Length=257, Percent_Identity=25.6809338521401, Blast_Score=65, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 35062; Mature: 35062

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMRHVMLLTTGGTIASTTSESGKLVAGELSGEELAKLCGLPEDIHVSVRSVLQKPSVHM
CCCEEEEEEEECCEEEECCCCCCCEEEECCCHHHHHHHHCCCHHHHHHHHHHHCCCCCEE
TLADWLLLKHEVERAFADETINGIVVTHGTDTLEETAYFLDLTNKDERPIIVTGSQRGPE
EHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHCEEEEEECCCCCCCEEEECCCCCCC
QTGSDAFMNLRHAIYAACEPDLKHTGCVVVFNERIFAARYVKKEHASNLQGFNAFGFGYL
CCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCEEE
GIIDNDVIFTYQKPVRREFYEIGNTPLKQIGIAKVYLGMSADMLEGMIAVCDGMVVEGVG
EEECCCEEEEECCHHHHHHHHHCCCCHHHHCHHHEEECCCHHHHHHHHHHHCCCEEECCC
RGQVPPNLVATLERAVANKKPIIVTTASEEGKVYPTYDYKGSAHQLEQMGVILGSDYDSK
CCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCEEECCCCCCCHHHHHHCCEEEECCCCCH
KARIKAMVMLAANKELAAGFT
HHHEEEEEEEECCCHHCCCCC
>Mature Secondary Structure
MNMRHVMLLTTGGTIASTTSESGKLVAGELSGEELAKLCGLPEDIHVSVRSVLQKPSVHM
CCCEEEEEEEECCEEEECCCCCCCEEEECCCHHHHHHHHCCCHHHHHHHHHHHCCCCCEE
TLADWLLLKHEVERAFADETINGIVVTHGTDTLEETAYFLDLTNKDERPIIVTGSQRGPE
EHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHCEEEEEECCCCCCCEEEECCCCCCC
QTGSDAFMNLRHAIYAACEPDLKHTGCVVVFNERIFAARYVKKEHASNLQGFNAFGFGYL
CCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCEEE
GIIDNDVIFTYQKPVRREFYEIGNTPLKQIGIAKVYLGMSADMLEGMIAVCDGMVVEGVG
EEECCCEEEEECCHHHHHHHHHCCCCHHHHCHHHEEECCCHHHHHHHHHHHCCCEEECCC
RGQVPPNLVATLERAVANKKPIIVTTASEEGKVYPTYDYKGSAHQLEQMGVILGSDYDSK
CCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCEEECCCCCCCHHHHHHCCEEEECCCCCH
KARIKAMVMLAANKELAAGFT
HHHEEEEEEEECCCHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1916233 [H]