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The map label for this gene is dacB [H]

Identifier: 56963593

GI number: 56963593

Start: 1945293

End: 1946435

Strand: Direct

Name: dacB [H]

Synonym: ABC1828

Alternate gene names: 56963593

Gene position: 1945293-1946435 (Clockwise)

Preceding gene: 56963592

Following gene: 56963594

Centisome position: 45.2

GC content: 44.53

Gene sequence:

>1143_bases
ATGAAAAAAACAATCGTTCACTGTTTATTGGTCGGGTTCTTGTTTTTTTTCCAGAGTAGCGAGGTGCTTGCGAATGAACC
TGCAACATTTCCTGTTTCTGGACAGGCCGCTATTTTGATGGAACAGTCTTCTGGCCGTGTTCTGTATAGCAAAAATGAAC
ATCAGCCTTTGAAAATCGCCAGCATTACGAAGATAATGACAGCCATCTTAGCGATTGAATCAGGCAAGCTAGACGAGACG
GTAACCGTATCTAAACGTGCTGAGGGAACAGAAGGTTCCTCCCTGTATTTAGTGGCTGGTGAAAAATTAACGCTCCGCGA
CTTGGTGTATGGGCTTATGCTACGGAGCGGCAACGACGCAGCTATAGCGATTGCTGAACATGTTGGCGGCAGCGTCGAAG
GCTTTGTATTTTTAATGAACGAAAAGGCAGAGGAAATTGGCATGTCTAATACGCTATTTCGCAATCCACACGGGCTTGAT
ACAGATGAGGACCACTTGTCGAGTGCGTACGACATGGCGCTTTTAACGCAATATGCGATGGGGAACGAAGAATACCGAGA
TATTTCATCGACAAAAGACTACCGTTCAAAAGGCGATAAAGTCCGTGTATTTCATAATAAAAACCGCCTCCTTACCGAGA
AGTACAGTTACTCAACCGGCGGGAAAACAGGTTATACGAAATTAGCAAAACGAACGCTTGTGTCTACCGCTTCCAAGGAC
GGGCTTGATTTAATTGCCGTTACGTTAAATGCCCCTGATGACTGGGACGACCATATGCATATGTTTAACTGGGGCTTTGA
GCATTACAGCCTTGAACGGCTCGTGTCAAAAGGGAAACTGAAAAGCAAAGTCGATCCATTTTACGAAGGCAATTTGTATG
TCCCGTATGATGTAGTGTATCCGCTAACGACAGAAGAAAAAAGTCAGTTGCAGTCTGCTTTAACGCTGAAGAATCCCCCG
AAAAGAGGAAAGTTTACTGACCAGGAGCTGCTTGAACCAATTGGAAAGTTAGCGTTTACGGTTGATGGCAAAACAATTGA
ATCAATCCCTCTTTATTATCAGGCTCAAGAAGAGGAGCACGTGTCTTTGTTTCAAAAAATTGGGCGGTTGCTAACATTCA
TCATTGGGGTGCGGCCTTTATGA

Upstream 100 bases:

>100_bases
TGAAAAGGTTTTTGGTCGTGGTTGAATTCGAACAAGCCCGTCATATTCATAGCTTGTTCGCATAAAGTGGTACTAAACGG
AGTGGAGGCTGGAGGGTATG

Downstream 100 bases:

>100_bases
TTAACGTGATTTGGGCCGGTATGCTATTGATCGGTCTTATCTATGCAGGTGTGAACGGGACGATGGCAGAGGTGACAGAG
AGCGTCTTCACCGGGGCGAA

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5*; PBP-5* [H]

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MKKTIVHCLLVGFLFFFQSSEVLANEPATFPVSGQAAILMEQSSGRVLYSKNEHQPLKIASITKIMTAILAIESGKLDET
VTVSKRAEGTEGSSLYLVAGEKLTLRDLVYGLMLRSGNDAAIAIAEHVGGSVEGFVFLMNEKAEEIGMSNTLFRNPHGLD
TDEDHLSSAYDMALLTQYAMGNEEYRDISSTKDYRSKGDKVRVFHNKNRLLTEKYSYSTGGKTGYTKLAKRTLVSTASKD
GLDLIAVTLNAPDDWDDHMHMFNWGFEHYSLERLVSKGKLKSKVDPFYEGNLYVPYDVVYPLTTEEKSQLQSALTLKNPP
KRGKFTDQELLEPIGKLAFTVDGKTIESIPLYYQAQEEEHVSLFQKIGRLLTFIIGVRPL

Sequences:

>Translated_380_residues
MKKTIVHCLLVGFLFFFQSSEVLANEPATFPVSGQAAILMEQSSGRVLYSKNEHQPLKIASITKIMTAILAIESGKLDET
VTVSKRAEGTEGSSLYLVAGEKLTLRDLVYGLMLRSGNDAAIAIAEHVGGSVEGFVFLMNEKAEEIGMSNTLFRNPHGLD
TDEDHLSSAYDMALLTQYAMGNEEYRDISSTKDYRSKGDKVRVFHNKNRLLTEKYSYSTGGKTGYTKLAKRTLVSTASKD
GLDLIAVTLNAPDDWDDHMHMFNWGFEHYSLERLVSKGKLKSKVDPFYEGNLYVPYDVVYPLTTEEKSQLQSALTLKNPP
KRGKFTDQELLEPIGKLAFTVDGKTIESIPLYYQAQEEEHVSLFQKIGRLLTFIIGVRPL
>Mature_380_residues
MKKTIVHCLLVGFLFFFQSSEVLANEPATFPVSGQAAILMEQSSGRVLYSKNEHQPLKIASITKIMTAILAIESGKLDET
VTVSKRAEGTEGSSLYLVAGEKLTLRDLVYGLMLRSGNDAAIAIAEHVGGSVEGFVFLMNEKAEEIGMSNTLFRNPHGLD
TDEDHLSSAYDMALLTQYAMGNEEYRDISSTKDYRSKGDKVRVFHNKNRLLTEKYSYSTGGKTGYTKLAKRTLVSTASKD
GLDLIAVTLNAPDDWDDHMHMFNWGFEHYSLERLVSKGKLKSKVDPFYEGNLYVPYDVVYPLTTEEKSQLQSALTLKNPP
KRGKFTDQELLEPIGKLAFTVDGKTIESIPLYYQAQEEEHVSLFQKIGRLLTFIIGVRPL

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. Required specifically for the synthesis of the spore form of peptidoglycan (cortex) [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Forespore outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=393, Percent_Identity=32.824427480916, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI87082022, Length=299, Percent_Identity=32.4414715719064, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1786851, Length=362, Percent_Identity=30.939226519337, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI87082054, Length=212, Percent_Identity=28.3018867924528, Blast_Score=73, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 42464; Mature: 42464

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTIVHCLLVGFLFFFQSSEVLANEPATFPVSGQAAILMEQSSGRVLYSKNEHQPLKIA
CCHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCEEEEEECCCCCEEEECCCCCCEEHH
SITKIMTAILAIESGKLDETVTVSKRAEGTEGSSLYLVAGEKLTLRDLVYGLMLRSGNDA
HHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCEEEEEECCCEEHHHHHHHHHHCCCCCC
AIAIAEHVGGSVEGFVFLMNEKAEEIGMSNTLFRNPHGLDTDEDHLSSAYDMALLTQYAM
EEEEHHHCCCCCCEEEEEEECCHHHCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHC
GNEEYRDISSTKDYRSKGDKVRVFHNKNRLLTEKYSYSTGGKTGYTKLAKRTLVSTASKD
CCHHHHHCCCCHHHHHCCCEEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCC
GLDLIAVTLNAPDDWDDHMHMFNWGFEHYSLERLVSKGKLKSKVDPFYEGNLYVPYDVVY
CCEEEEEEECCCCCCCCCHHHHHCCHHHHHHHHHHHCCCCHHCCCCCCCCCEEECEEEEE
PLTTEEKSQLQSALTLKNPPKRGKFTDQELLEPIGKLAFTVDGKTIESIPLYYQAQEEEH
ECCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHEEEEECCCEEECCCEEEECCCHHH
VSLFQKIGRLLTFIIGVRPL
HHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKKTIVHCLLVGFLFFFQSSEVLANEPATFPVSGQAAILMEQSSGRVLYSKNEHQPLKIA
CCHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCEEEEEECCCCCEEEECCCCCCEEHH
SITKIMTAILAIESGKLDETVTVSKRAEGTEGSSLYLVAGEKLTLRDLVYGLMLRSGNDA
HHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCEEEEEECCCEEHHHHHHHHHHCCCCCC
AIAIAEHVGGSVEGFVFLMNEKAEEIGMSNTLFRNPHGLDTDEDHLSSAYDMALLTQYAM
EEEEHHHCCCCCCEEEEEEECCHHHCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHC
GNEEYRDISSTKDYRSKGDKVRVFHNKNRLLTEKYSYSTGGKTGYTKLAKRTLVSTASKD
CCHHHHHCCCCHHHHHCCCEEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCC
GLDLIAVTLNAPDDWDDHMHMFNWGFEHYSLERLVSKGKLKSKVDPFYEGNLYVPYDVVY
CCEEEEEEECCCCCCCCCHHHHHCCHHHHHHHHHHHCCCCHHCCCCCCCCCEEECEEEEE
PLTTEEKSQLQSALTLKNPPKRGKFTDQELLEPIGKLAFTVDGKTIESIPLYYQAQEEEH
ECCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHEEEEECCCEEECCCEEEECCCHHH
VSLFQKIGRLLTFIIGVRPL
HHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1548223; 7934829; 9384377 [H]