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The map label for this gene is prpB [H]

Identifier: 56963572

GI number: 56963572

Start: 1925064

End: 1925972

Strand: Direct

Name: prpB [H]

Synonym: ABC1807

Alternate gene names: 56963572

Gene position: 1925064-1925972 (Clockwise)

Preceding gene: 56963571

Following gene: 56963573

Centisome position: 44.73

GC content: 47.52

Gene sequence:

>909_bases
ATGCCTTGGATTGTAGACGAACCGCGCTCACAACAAGAACTAGCAACGTCTTTTTACAAACAGATGACGGAAAAGGGAAT
CTTACAAATTCCAGGTGCACATGATGCTATGGCTGGCTTAATCGCAAAACAAACGGGCTTTACTAGCCTTTATTTGTCAG
GAGCTGCTTATACGGCGAGTTTAGGATTGCCTGATCTTGGAATTGTCTATTCAAACGAAGTGGCTGTCCGAGCCCGGGAA
ATAGTCCGGGCTACAAACCTGCCGCTTTTAGTCGATATCGATACTGGATTTGGCGGTGTGATGAACGTTGCTCGAACAGG
GGTGGAAATGGTTGAAGCTGGGGTTGCGGCTGTACAGATGGAAGACCAAGACTTGCCGAAAAAATGTGGCCATTTGAACG
GAAAAAAACTGATTCAGGCTGAAGAGATGGCGCAAAAAATTGCTGCTTTGAAAAAAGCGGCTCCGTCGCTTGTCATCATT
GCCCGGACCGATGCAAGGGGCGTGGAAGGGCTTCAGTCAGCAATTGACCGCGCCAAGCTTTATCTCGATGCCGGCGCTGA
TGGCATTTTCCCAGAAGCGTTGCAAGGTGAAGAAGAGTTCCGTGCCTTTGCCGAAGCAGTCGAAGCGCCTTTGCTTGCGA
ATATGACGGAGTTTGGCAAAACGCCTTATTACAAAGCAGAGGAATTTGAACAGATGGGGTTTGCGATGGTCATTTATCCC
GTCACATCGCTTCGCGTAGCGGCCAAAGCATATGAACGTGTGTTTACTGATATTATGGAGAATGGTACGCAAAAAGCGAG
TTTGGCTGACATGCAAAAGCGCAGCGAATTATACGAAACCATTTCATATGATGAATTTGAAGCGTTGGATGCGTCTGTCG
CCAAAACGATTTTAACAAGCGACCAGTAA

Upstream 100 bases:

>100_bases
AAAGCAATCCTTGAGGCTTGTACAAACAAAGACTTAGGCAACATGTCAGTACCAGCTTTTACAGACTTGCTTGCCTTATA
GAAAGAGAGGAGGGATTGCA

Downstream 100 bases:

>100_bases
TACGTTGCAAAGTCATACGGGTTGGTAGAAAACGCTTGTCTGTTAAATACAAATAACACGGGTGTGCTTACAGAAATGAT
CGTTTCAAAGTATATGAACG

Product: carboxyvinyl-carboxyphosphonate phosphorylmutase

Products: NA

Alternate protein names: 2-methylisocitrate lyase [H]

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MPWIVDEPRSQQELATSFYKQMTEKGILQIPGAHDAMAGLIAKQTGFTSLYLSGAAYTASLGLPDLGIVYSNEVAVRARE
IVRATNLPLLVDIDTGFGGVMNVARTGVEMVEAGVAAVQMEDQDLPKKCGHLNGKKLIQAEEMAQKIAALKKAAPSLVII
ARTDARGVEGLQSAIDRAKLYLDAGADGIFPEALQGEEEFRAFAEAVEAPLLANMTEFGKTPYYKAEEFEQMGFAMVIYP
VTSLRVAAKAYERVFTDIMENGTQKASLADMQKRSELYETISYDEFEALDASVAKTILTSDQ

Sequences:

>Translated_302_residues
MPWIVDEPRSQQELATSFYKQMTEKGILQIPGAHDAMAGLIAKQTGFTSLYLSGAAYTASLGLPDLGIVYSNEVAVRARE
IVRATNLPLLVDIDTGFGGVMNVARTGVEMVEAGVAAVQMEDQDLPKKCGHLNGKKLIQAEEMAQKIAALKKAAPSLVII
ARTDARGVEGLQSAIDRAKLYLDAGADGIFPEALQGEEEFRAFAEAVEAPLLANMTEFGKTPYYKAEEFEQMGFAMVIYP
VTSLRVAAKAYERVFTDIMENGTQKASLADMQKRSELYETISYDEFEALDASVAKTILTSDQ
>Mature_301_residues
PWIVDEPRSQQELATSFYKQMTEKGILQIPGAHDAMAGLIAKQTGFTSLYLSGAAYTASLGLPDLGIVYSNEVAVRAREI
VRATNLPLLVDIDTGFGGVMNVARTGVEMVEAGVAAVQMEDQDLPKKCGHLNGKKLIQAEEMAQKIAALKKAAPSLVIIA
RTDARGVEGLQSAIDRAKLYLDAGADGIFPEALQGEEEFRAFAEAVEAPLLANMTEFGKTPYYKAEEFEQMGFAMVIYPV
TSLRVAAKAYERVFTDIMENGTQKASLADMQKRSELYETISYDEFEALDASVAKTILTSDQ

Specific function: Catalyzes the formation of pyruvate and succinate from 2-methylisocitrate [H]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1786525, Length=275, Percent_Identity=44.3636363636364, Blast_Score=231, Evalue=3e-62,
Organism=Escherichia coli, GI1790445, Length=187, Percent_Identity=32.620320855615, Blast_Score=75, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6320908, Length=104, Percent_Identity=32.6923076923077, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR012695
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.30 [H]

Molecular weight: Translated: 32837; Mature: 32706

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPWIVDEPRSQQELATSFYKQMTEKGILQIPGAHDAMAGLIAKQTGFTSLYLSGAAYTAS
CCCCCCCCCCHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHCCCCEEEECCCCEEEE
LGLPDLGIVYSNEVAVRAREIVRATNLPLLVDIDTGFGGVMNVARTGVEMVEAGVAAVQM
CCCCCCCEEECCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHEEE
EDQDLPKKCGHLNGKKLIQAEEMAQKIAALKKAAPSLVIIARTDARGVEGLQSAIDRAKL
CCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHE
YLDAGADGIFPEALQGEEEFRAFAEAVEAPLLANMTEFGKTPYYKAEEFEQMGFAMVIYP
EEECCCCCCCHHHHCCHHHHHHHHHHHHCHHHHHHHHHCCCCCCCHHHHHHCCCEEEEEH
VTSLRVAAKAYERVFTDIMENGTQKASLADMQKRSELYETISYDEFEALDASVAKTILTS
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
DQ
CC
>Mature Secondary Structure 
PWIVDEPRSQQELATSFYKQMTEKGILQIPGAHDAMAGLIAKQTGFTSLYLSGAAYTAS
CCCCCCCCCHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHCCCCEEEECCCCEEEE
LGLPDLGIVYSNEVAVRAREIVRATNLPLLVDIDTGFGGVMNVARTGVEMVEAGVAAVQM
CCCCCCCEEECCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHEEE
EDQDLPKKCGHLNGKKLIQAEEMAQKIAALKKAAPSLVIIARTDARGVEGLQSAIDRAKL
CCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHE
YLDAGADGIFPEALQGEEEFRAFAEAVEAPLLANMTEFGKTPYYKAEEFEQMGFAMVIYP
EEECCCCCCCHHHHCCHHHHHHHHHHHHCHHHHHHHHHCCCCCCCHHHHHHCCCEEEEEH
VTSLRVAAKAYERVFTDIMENGTQKASLADMQKRSELYETISYDEFEALDASVAKTILTS
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
DQ
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10086842; 11058132 [H]