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The map label for this gene is prpD [H]

Identifier: 56963571

GI number: 56963571

Start: 1923611

End: 1925044

Strand: Direct

Name: prpD [H]

Synonym: ABC1806

Alternate gene names: 56963571

Gene position: 1923611-1925044 (Clockwise)

Preceding gene: 56963570

Following gene: 56963572

Centisome position: 44.69

GC content: 48.54

Gene sequence:

>1434_bases
ATGGACTTGAAAACGGATACAAGAAAAACAGACTTGCTTTTAGAGGAAATTGCTGAGTATGTGCTAAAAAAGGAGATTAC
AAGCGAGGAGGCGCTGGAAACCGCGGCGCACGTCCTTGTTGATACGATTGGCTGCGGCATTCTGGCCCTCCGCTATCCGG
AATGCGTGAAAATGCTCGGCCCGGTTGTGCCGGGGACGATTGTTCCAAATGGGGTTAAAGTTCCTGGCACATCCCATGTT
TTAGACCCGGTTCGGGGCGCTTTTAATATTGGTACGATGATACGGTGGCTTGATTACAACGATACGTGGCTTGCAGCTGA
GTGGGGGCACCCATCGGATAACCTTGGCGGCATTTTAGCGACGGCCGACTATATAAGCAGAAAAAACATAGCAGAAGGCA
AAGCGCCTCTAACGATCCATGATGTGTTAATCGCGATGGTAAAAGCCCATGAAATCCAAGGCGTGCTTGCATTAGAAAAC
AGCTTTAACCGAGTTGGCCTCGACCATGTGCTCCTCGTTAAAGTAGCGACTACCGCTGTGGTTGCCGGGATGCTTGGTGG
TACAAAAGAAGAAGTCGTAAACGCCGTTTCCCATGCCTGGATTGACAATTCCTCGTTGCGCACATACCGCCACGCTCCGA
ACACAGGCTCGCGGAAATCATGGGCGGCTGGCGACGCGACAAGCCGAGGCGTCTTTTTGGCAATGACAGCTTTAACAGGC
GAGATGGGCTATAAAACGGCGCTGTCTGCTCCTGGATGGGGTTTCCAAGACGTGCTTTTTAATAAGAAGGAAGTCGTGCT
CGCCCGTAAGCTGGACTCATATGTCATGGAAAATGTGTTATTTAAAGTGTCTTATCCAGCTGAATTCCACGCGCAAACGG
CAGCTGAAGCGGCAGTCACGTTACATGAGTCAGTCCGTGACCGTCTGGATGAGATCGACCGTATTGAAATCCATACCCAT
GAATCGGCGATCCGCATCATTGACAAAAAAGGGCCGCTGTACAATCCTGCTGATCGGGATCATTGTTTGCAATACATTAC
AGCAATTGGCCTGTTAAAGGGTGCAATTGTCGCAGATGACTATGAAGATGAGATGGCCCAAGATCCCCGTGTGGACCAAC
TTCGCGATTTGATGGTGGTTACGGAAAACAAACAGTTTTCAATAGACTACTTAGATCCAGAAAAACGGTCGATTGCCAAT
GCTGTGCAAGTCTTTTTTAAGGACGGTACGAAAACGGACCAAGTAACAATCGAATATCCACTTGGCCATCGTTTCCGCCG
CGAAGAAGCTGTTCCTGCTATTCAGGAGAAATTCGCAGCAAATATCGCTAGCCATTATCCGCAAAAACAAGAAAAAGCAA
TCCTTGAGGCTTGTACAAACAAAGACTTAGGCAACATGTCAGTACCAGCTTTTACAGACTTGCTTGCCTTATAG

Upstream 100 bases:

>100_bases
GAGCAACATCGAAACAACAAGCTATTTAGGCCTCGGGTTAAGTATACAGGGCCACGTTATACATCTGTACAGAAATGAAG
GGACAACGGAGGTTTTTTGC

Downstream 100 bases:

>100_bases
AAAGAGAGGAGGGATTGCAATGCCTTGGATTGTAGACGAACCGCGCTCACAACAAGAACTAGCAACGTCTTTTTACAAAC
AGATGACGGAAAAGGGAATC

Product: 2-methylcitrate dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MDLKTDTRKTDLLLEEIAEYVLKKEITSEEALETAAHVLVDTIGCGILALRYPECVKMLGPVVPGTIVPNGVKVPGTSHV
LDPVRGAFNIGTMIRWLDYNDTWLAAEWGHPSDNLGGILATADYISRKNIAEGKAPLTIHDVLIAMVKAHEIQGVLALEN
SFNRVGLDHVLLVKVATTAVVAGMLGGTKEEVVNAVSHAWIDNSSLRTYRHAPNTGSRKSWAAGDATSRGVFLAMTALTG
EMGYKTALSAPGWGFQDVLFNKKEVVLARKLDSYVMENVLFKVSYPAEFHAQTAAEAAVTLHESVRDRLDEIDRIEIHTH
ESAIRIIDKKGPLYNPADRDHCLQYITAIGLLKGAIVADDYEDEMAQDPRVDQLRDLMVVTENKQFSIDYLDPEKRSIAN
AVQVFFKDGTKTDQVTIEYPLGHRFRREEAVPAIQEKFAANIASHYPQKQEKAILEACTNKDLGNMSVPAFTDLLAL

Sequences:

>Translated_477_residues
MDLKTDTRKTDLLLEEIAEYVLKKEITSEEALETAAHVLVDTIGCGILALRYPECVKMLGPVVPGTIVPNGVKVPGTSHV
LDPVRGAFNIGTMIRWLDYNDTWLAAEWGHPSDNLGGILATADYISRKNIAEGKAPLTIHDVLIAMVKAHEIQGVLALEN
SFNRVGLDHVLLVKVATTAVVAGMLGGTKEEVVNAVSHAWIDNSSLRTYRHAPNTGSRKSWAAGDATSRGVFLAMTALTG
EMGYKTALSAPGWGFQDVLFNKKEVVLARKLDSYVMENVLFKVSYPAEFHAQTAAEAAVTLHESVRDRLDEIDRIEIHTH
ESAIRIIDKKGPLYNPADRDHCLQYITAIGLLKGAIVADDYEDEMAQDPRVDQLRDLMVVTENKQFSIDYLDPEKRSIAN
AVQVFFKDGTKTDQVTIEYPLGHRFRREEAVPAIQEKFAANIASHYPQKQEKAILEACTNKDLGNMSVPAFTDLLAL
>Mature_477_residues
MDLKTDTRKTDLLLEEIAEYVLKKEITSEEALETAAHVLVDTIGCGILALRYPECVKMLGPVVPGTIVPNGVKVPGTSHV
LDPVRGAFNIGTMIRWLDYNDTWLAAEWGHPSDNLGGILATADYISRKNIAEGKAPLTIHDVLIAMVKAHEIQGVLALEN
SFNRVGLDHVLLVKVATTAVVAGMLGGTKEEVVNAVSHAWIDNSSLRTYRHAPNTGSRKSWAAGDATSRGVFLAMTALTG
EMGYKTALSAPGWGFQDVLFNKKEVVLARKLDSYVMENVLFKVSYPAEFHAQTAAEAAVTLHESVRDRLDEIDRIEIHTH
ESAIRIIDKKGPLYNPADRDHCLQYITAIGLLKGAIVADDYEDEMAQDPRVDQLRDLMVVTENKQFSIDYLDPEKRSIAN
AVQVFFKDGTKTDQVTIEYPLGHRFRREEAVPAIQEKFAANIASHYPQKQEKAILEACTNKDLGNMSVPAFTDLLAL

Specific function: Catalyzes the dehydration of 2-methylcitrate to 2- methyl-cis-aconitate [H]

COG id: COG2079

COG function: function code R; Uncharacterized protein involved in propionate catabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prpD family [H]

Homologues:

Organism=Escherichia coli, GI1786528, Length=466, Percent_Identity=61.3733905579399, Blast_Score=606, Evalue=1e-175,
Organism=Saccharomyces cerevisiae, GI6325258, Length=486, Percent_Identity=52.4691358024691, Blast_Score=519, Evalue=1e-148,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012705
- InterPro:   IPR005656 [H]

Pfam domain/function: PF03972 MmgE_PrpD [H]

EC number: =4.2.1.79 [H]

Molecular weight: Translated: 52602; Mature: 52602

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLKTDTRKTDLLLEEIAEYVLKKEITSEEALETAAHVLVDTIGCGILALRYPECVKMLG
CCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHC
PVVPGTIVPNGVKVPGTSHVLDPVRGAFNIGTMIRWLDYNDTWLAAEWGHPSDNLGGILA
CCCCCCCCCCCEECCCCCHHHHHHHHHHHHCCEEEEECCCCCEEEEECCCCCCCCCCHHH
TADYISRKNIAEGKAPLTIHDVLIAMVKAHEIQGVLALENSFNRVGLDHVLLVKVATTAV
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCHHHHHHHHHHHH
VAGMLGGTKEEVVNAVSHAWIDNSSLRTYRHAPNTGSRKSWAAGDATSRGVFLAMTALTG
HHHHHCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCCCCCEEEEEEHHHC
EMGYKTALSAPGWGFQDVLFNKKEVVLARKLDSYVMENVLFKVSYPAEFHAQTAAEAAVT
CCCCHHCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHH
LHESVRDRLDEIDRIEIHTHESAIRIIDKKGPLYNPADRDHCLQYITAIGLLKGAIVADD
HHHHHHHHHCCCCEEEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCEECC
YEDEMAQDPRVDQLRDLMVVTENKQFSIDYLDPEKRSIANAVQVFFKDGTKTDQVTIEYP
CHHHHHCCCCHHHHHCEEEEECCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEEEEC
LGHRFRREEAVPAIQEKFAANIASHYPQKQEKAILEACTNKDLGNMSVPAFTDLLAL
CCCHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHCC
>Mature Secondary Structure
MDLKTDTRKTDLLLEEIAEYVLKKEITSEEALETAAHVLVDTIGCGILALRYPECVKMLG
CCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHC
PVVPGTIVPNGVKVPGTSHVLDPVRGAFNIGTMIRWLDYNDTWLAAEWGHPSDNLGGILA
CCCCCCCCCCCEECCCCCHHHHHHHHHHHHCCEEEEECCCCCEEEEECCCCCCCCCCHHH
TADYISRKNIAEGKAPLTIHDVLIAMVKAHEIQGVLALENSFNRVGLDHVLLVKVATTAV
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCHHHHHHHHHHHH
VAGMLGGTKEEVVNAVSHAWIDNSSLRTYRHAPNTGSRKSWAAGDATSRGVFLAMTALTG
HHHHHCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCCCCCEEEEEEHHHC
EMGYKTALSAPGWGFQDVLFNKKEVVLARKLDSYVMENVLFKVSYPAEFHAQTAAEAAVT
CCCCHHCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHH
LHESVRDRLDEIDRIEIHTHESAIRIIDKKGPLYNPADRDHCLQYITAIGLLKGAIVADD
HHHHHHHHHCCCCEEEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCEECC
YEDEMAQDPRVDQLRDLMVVTENKQFSIDYLDPEKRSIANAVQVFFKDGTKTDQVTIEYP
CHHHHHCCCCHHHHHCEEEEECCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEEEEC
LGHRFRREEAVPAIQEKFAANIASHYPQKQEKAILEACTNKDLGNMSVPAFTDLLAL
CCCHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 8759838 [H]