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The map label for this gene is dacF [H]

Identifier: 56963557

GI number: 56963557

Start: 1910077

End: 1911243

Strand: Direct

Name: dacF [H]

Synonym: ABC1792

Alternate gene names: 56963557

Gene position: 1910077-1911243 (Clockwise)

Preceding gene: 56963556

Following gene: 56963558

Centisome position: 44.38

GC content: 44.64

Gene sequence:

>1167_bases
ATGAAAAGAAAATGGAACGTATCGATGCTCCTGTTTGTTTTGTTGTTTGCATACGCTGGTGAAGCAGCCTTTGCAAAAGA
AAAGGAACTGCCTCAGTTAGCTGAAAATGCCGCGTCCGCGATATTAATCGAACGGGATACAGGCGAAGTATTGTTTGAAA
AAGACAGCGACAACATGCTTCCCCCGGCTAGCATGACGAAAATCATGACCATGCTGCTTATTATGGAAGCAATTGAAGAA
GGCACGATTAAGTATGAGGATATGGTGACCGTTAGCGAGAACGCTGCTTCCATGGGCGGATCGCAAGTCTTTTTAGAACC
TGGGGAAGAAATGAGTGTGGATGACTTATTGAAAGCCATTGCCATTGCTTCTGGGAATGATGCAGCGGTAGCGATGGCTG
AACATATTGGCGGCACAGAACAAGAGTTTGTAAAAATGATGAATGCAAAAGCGGCAGAGCTTGGTTTGGAAAATACACAG
TTTCAAAATCCGAATGGCTTGCCTGAAGCGGACCATTACTCTTCTGCACATGATTTAGCTGTTATGGCGAAAGAGTTGCT
TAAATATGAAAACATCACTTCGTATACAGGGATATACGAAGATTATTTACGAAAAGGTACCGATAAGGAGTTTTGGCTCG
TAAACACGAATAAGCTCGTCAAGTTTTACCCTGGAGTCGACGGCTTGAAAACTGGGTTTACACAAGAGGCAAAGTATGGG
TTGACAGCCACAGCAGAGAAGAACGGGATGCGTGTTATTGCCGTCGTTATGGGGGCGAAAACGCCGAAAGAGCGCAATGC
TACAATAACGAACATGCTTGACTATTCATATAGCCACTATTCAACGGAGCGGTTGCATGAGCGAGGAGACCTTATTGGGG
AAGTCCCGGTTATAAAAGGCAAGGATAGGCATGTACAAGCCATCACGCCTGAGCCCGTTTCATTGTTGTTGAAAAAAGGC
ACGAAGCTTGAGGAAATTGAGACGAAAATCGAGCTATTTGAAAACGTGGAAGCACCTGTCACAAAAGGGGATGCAGTTGG
CCAGCTAATTATTGTAAGAGATGGCGAAACACTTTCAAGAACAGACCTTGTGGCAGCTGAAGATGTAGAAAACGCTTCGT
GGTGGACGCTGTTTAAACGGATGTTGTCATCATTTGCTGGAAAATAG

Upstream 100 bases:

>100_bases
AGCGTTTTAAATATTGTTTGGGCAAATGTATAACCTTTCCAGTAAATGGAGACACTAGCAAATAGTTTTTTTATGATGAC
GGCCTAGTGGAGGGAAAAAC

Downstream 100 bases:

>100_bases
CTAATCACAAAGGAAAAGCAAACTTTGCCACTAGTTTTGTCATGTACAAGGAATCTGTCATTTTGCAGGCGAATGAATTA
CTAGCAAAACGACGGAGGTG

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP [H]

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MKRKWNVSMLLFVLLFAYAGEAAFAKEKELPQLAENAASAILIERDTGEVLFEKDSDNMLPPASMTKIMTMLLIMEAIEE
GTIKYEDMVTVSENAASMGGSQVFLEPGEEMSVDDLLKAIAIASGNDAAVAMAEHIGGTEQEFVKMMNAKAAELGLENTQ
FQNPNGLPEADHYSSAHDLAVMAKELLKYENITSYTGIYEDYLRKGTDKEFWLVNTNKLVKFYPGVDGLKTGFTQEAKYG
LTATAEKNGMRVIAVVMGAKTPKERNATITNMLDYSYSHYSTERLHERGDLIGEVPVIKGKDRHVQAITPEPVSLLLKKG
TKLEEIETKIELFENVEAPVTKGDAVGQLIIVRDGETLSRTDLVAAEDVENASWWTLFKRMLSSFAGK

Sequences:

>Translated_388_residues
MKRKWNVSMLLFVLLFAYAGEAAFAKEKELPQLAENAASAILIERDTGEVLFEKDSDNMLPPASMTKIMTMLLIMEAIEE
GTIKYEDMVTVSENAASMGGSQVFLEPGEEMSVDDLLKAIAIASGNDAAVAMAEHIGGTEQEFVKMMNAKAAELGLENTQ
FQNPNGLPEADHYSSAHDLAVMAKELLKYENITSYTGIYEDYLRKGTDKEFWLVNTNKLVKFYPGVDGLKTGFTQEAKYG
LTATAEKNGMRVIAVVMGAKTPKERNATITNMLDYSYSHYSTERLHERGDLIGEVPVIKGKDRHVQAITPEPVSLLLKKG
TKLEEIETKIELFENVEAPVTKGDAVGQLIIVRDGETLSRTDLVAAEDVENASWWTLFKRMLSSFAGK
>Mature_388_residues
MKRKWNVSMLLFVLLFAYAGEAAFAKEKELPQLAENAASAILIERDTGEVLFEKDSDNMLPPASMTKIMTMLLIMEAIEE
GTIKYEDMVTVSENAASMGGSQVFLEPGEEMSVDDLLKAIAIASGNDAAVAMAEHIGGTEQEFVKMMNAKAAELGLENTQ
FQNPNGLPEADHYSSAHDLAVMAKELLKYENITSYTGIYEDYLRKGTDKEFWLVNTNKLVKFYPGVDGLKTGFTQEAKYG
LTATAEKNGMRVIAVVMGAKTPKERNATITNMLDYSYSHYSTERLHERGDLIGEVPVIKGKDRHVQAITPEPVSLLLKKG
TKLEEIETKIELFENVEAPVTKGDAVGQLIIVRDGETLSRTDLVAAEDVENASWWTLFKRMLSSFAGK

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=371, Percent_Identity=32.8840970350404, Blast_Score=182, Evalue=4e-47,
Organism=Escherichia coli, GI1786851, Length=359, Percent_Identity=32.3119777158774, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI87082022, Length=403, Percent_Identity=29.7766749379653, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI87082054, Length=232, Percent_Identity=31.0344827586207, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 42888; Mature: 42888

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRKWNVSMLLFVLLFAYAGEAAFAKEKELPQLAENAASAILIERDTGEVLFEKDSDNML
CCCCCCHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHCCCEEEEECCCCCEEEECCCCCCC
PPASMTKIMTMLLIMEAIEEGTIKYEDMVTVSENAASMGGSQVFLEPGEEMSVDDLLKAI
CCHHHHHHHHHHHHHHHHHCCCEEEEHEEEECCCHHHCCCCEEEECCCCCCCHHHHHHHH
AIASGNDAAVAMAEHIGGTEQEFVKMMNAKAAELGLENTQFQNPNGLPEADHYSSAHDLA
HHCCCCCHHHHHHHHCCCCHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHH
VMAKELLKYENITSYTGIYEDYLRKGTDKEFWLVNTNKLVKFYPGVDGLKTGFTQEAKYG
HHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEECCCEEEECCCCCCHHCCCCCHHCCC
LTATAEKNGMRVIAVVMGAKTPKERNATITNMLDYSYSHYSTERLHERGDLIGEVPVIKG
EEEEECCCCCEEEEEEECCCCCCCCCCCHHHHHHHCHHHHHHHHHHHHCCCEECCCEECC
KDRHVQAITPEPVSLLLKKGTKLEEIETKIELFENVEAPVTKGDAVGQLIIVRDGETLSR
CCCEEEEECCCHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCH
TDLVAAEDVENASWWTLFKRMLSSFAGK
HHCEECCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKRKWNVSMLLFVLLFAYAGEAAFAKEKELPQLAENAASAILIERDTGEVLFEKDSDNML
CCCCCCHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHCCCEEEEECCCCCEEEECCCCCCC
PPASMTKIMTMLLIMEAIEEGTIKYEDMVTVSENAASMGGSQVFLEPGEEMSVDDLLKAI
CCHHHHHHHHHHHHHHHHHCCCEEEEHEEEECCCHHHCCCCEEEECCCCCCCHHHHHHHH
AIASGNDAAVAMAEHIGGTEQEFVKMMNAKAAELGLENTQFQNPNGLPEADHYSSAHDLA
HHCCCCCHHHHHHHHCCCCHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHH
VMAKELLKYENITSYTGIYEDYLRKGTDKEFWLVNTNKLVKFYPGVDGLKTGFTQEAKYG
HHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEECCCEEEECCCCCCHHCCCCCHHCCC
LTATAEKNGMRVIAVVMGAKTPKERNATITNMLDYSYSHYSTERLHERGDLIGEVPVIKG
EEEEECCCCCEEEEEEECCCCCCCCCCCHHHHHHHCHHHHHHHHHHHHCCCEECCCEECC
KDRHVQAITPEPVSLLLKKGTKLEEIETKIELFENVEAPVTKGDAVGQLIIVRDGETLSR
CCCEEEEECCCHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCH
TDLVAAEDVENASWWTLFKRMLSSFAGK
HHCEECCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377 [H]