Click here to switch to the map view.

The map label for this gene is yclM [H]

Identifier: 56963528

GI number: 56963528

Start: 1883262

End: 1884620

Strand: Direct

Name: yclM [H]

Synonym: ABC1763

Alternate gene names: 56963528

Gene position: 1883262-1884620 (Clockwise)

Preceding gene: 56963527

Following gene: 56963533

Centisome position: 43.76

GC content: 43.12

Gene sequence:

>1359_bases
GTGAAAGTAGCAAAATTTGGCGGTTCATCCGTCGCAAATGCAGAGCAAATTAAAAAAGTGGCTGCCATCATCAAAGCGGA
TAATAAACGGAAGATCGTGGTTGTTTCAGCACCAGGAAAGCGTTACGCTGATGATGCAAAAATGACAGACTTGCTCATTG
CCTTGGCCGAAGCTAAAGTGGCAGATCAGCCAGTAGAAGCAGCATTTAATGCTGTGATGAACAGGTATGAATCGATAGCA
AACGATTTAGAGCTTAATCGCGATGTGATCGCTGAAATTGAAGCAGATTTGAAAAACCGTCTTGAGCAAACAGTGACGGG
CAAAGGGCGCTTTCTTGATATAATGAAAGCTAGCGGAGAAGACAACAATGCAAAACTCATTGCTGCTTATTTAAGCAAAA
TCGGTCTAAAAGCTACGTACATAAACCCGAAAGATGCTGGACTTCTTGTTAGCGATGAACCAGGGAATGCCCAAGTGTTG
CCTGAAGCATACGAGGCGCTTGCCAAGTTGGCTGAAATAGACGGAATCGTCGTGTTTCCCGGTTTTTTTGGATTCTCAAA
ATTAGGAAATCTTGTTACTTTCCCAAGAGGCGGTTCCGACATTACAGGGGCTATATTGGCCGCTGCTGTTCAAGCCGAGC
TTTATGAAAATTTCACTGATGTTGATTCTGTTTACGCTGCGAATCCATTTATCGTTGAGGATCCAGTTGAAATCAAACGA
ATGACTTATCGGGAAATGCGCGAACTTTCTTACGCGGGCTTTTCCGTCTTTCATGATGAGGCCTTGATTCCGGCCTTCCG
CAACGACATTCCTGTATGTGTGAAAAATACAAACAATCCTAAATGCAGTGGCACATTAATTTTGGCGGAACGTGAGCGAT
CACTTAACCCTATTATTGGCATTGCTTCCGATGAAGGGTTTGCCACCATTTATGTACGAAAATATTTAATGAACCGCGAA
GTCGGATTTGGTCGCCATCTATTGCAAATTTTAGAAGATGCAGGGATTTCTTATGAGCATATTCCTTCTGGAATTGATGA
TACATCTGTGATTATCCGGCAAACGCAACTTTCTGATCAGTTGGAAAACGCCATTATTGAGCGTATTAAAGAGGAGCTTA
ACGTAGACGACGTCCACATTGAGCATGATTTTGCGATGGTCATGCTCGTTGGAGAAGGCATGCATAATACAGTAGGTGTA
ACAGCTAGAGCTTCATCTGCTTTGGCAAAAGCGGGGGTTAACATCGAAATGATTAACCAAGGCTCGTCTGAAGTTAGCCT
TGTGTTCGGCATTCATTCAAAAGATACTAAACAAGCTGTCCGCGTGCTTTATGAGGAGTTTTTCGGACAAAAGCAATAG

Upstream 100 bases:

>100_bases
AGACTGATAGAAAACGCTTGTCAAACGACGAAGTATGCGAGCGTTTGTCTACAGTCTGAAACGTTTTTTAGTTTAGAAAG
AGAGTGGGGCTGGAGAGAAC

Downstream 100 bases:

>100_bases
CCATATGTATGAAGACCAGTTGGTGCCAACTGGTCTTCGGTTGTTTTATGACGAACGTTCTTTTTTAAGCATTTCTTTTA
ACTCATCCAATTGCCGTTGG

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MKVAKFGGSSVANAEQIKKVAAIIKADNKRKIVVVSAPGKRYADDAKMTDLLIALAEAKVADQPVEAAFNAVMNRYESIA
NDLELNRDVIAEIEADLKNRLEQTVTGKGRFLDIMKASGEDNNAKLIAAYLSKIGLKATYINPKDAGLLVSDEPGNAQVL
PEAYEALAKLAEIDGIVVFPGFFGFSKLGNLVTFPRGGSDITGAILAAAVQAELYENFTDVDSVYAANPFIVEDPVEIKR
MTYREMRELSYAGFSVFHDEALIPAFRNDIPVCVKNTNNPKCSGTLILAERERSLNPIIGIASDEGFATIYVRKYLMNRE
VGFGRHLLQILEDAGISYEHIPSGIDDTSVIIRQTQLSDQLENAIIERIKEELNVDDVHIEHDFAMVMLVGEGMHNTVGV
TARASSALAKAGVNIEMINQGSSEVSLVFGIHSKDTKQAVRVLYEEFFGQKQ

Sequences:

>Translated_452_residues
MKVAKFGGSSVANAEQIKKVAAIIKADNKRKIVVVSAPGKRYADDAKMTDLLIALAEAKVADQPVEAAFNAVMNRYESIA
NDLELNRDVIAEIEADLKNRLEQTVTGKGRFLDIMKASGEDNNAKLIAAYLSKIGLKATYINPKDAGLLVSDEPGNAQVL
PEAYEALAKLAEIDGIVVFPGFFGFSKLGNLVTFPRGGSDITGAILAAAVQAELYENFTDVDSVYAANPFIVEDPVEIKR
MTYREMRELSYAGFSVFHDEALIPAFRNDIPVCVKNTNNPKCSGTLILAERERSLNPIIGIASDEGFATIYVRKYLMNRE
VGFGRHLLQILEDAGISYEHIPSGIDDTSVIIRQTQLSDQLENAIIERIKEELNVDDVHIEHDFAMVMLVGEGMHNTVGV
TARASSALAKAGVNIEMINQGSSEVSLVFGIHSKDTKQAVRVLYEEFFGQKQ
>Mature_452_residues
MKVAKFGGSSVANAEQIKKVAAIIKADNKRKIVVVSAPGKRYADDAKMTDLLIALAEAKVADQPVEAAFNAVMNRYESIA
NDLELNRDVIAEIEADLKNRLEQTVTGKGRFLDIMKASGEDNNAKLIAAYLSKIGLKATYINPKDAGLLVSDEPGNAQVL
PEAYEALAKLAEIDGIVVFPGFFGFSKLGNLVTFPRGGSDITGAILAAAVQAELYENFTDVDSVYAANPFIVEDPVEIKR
MTYREMRELSYAGFSVFHDEALIPAFRNDIPVCVKNTNNPKCSGTLILAERERSLNPIIGIASDEGFATIYVRKYLMNRE
VGFGRHLLQILEDAGISYEHIPSGIDDTSVIIRQTQLSDQLENAIIERIKEELNVDDVHIEHDFAMVMLVGEGMHNTVGV
TARASSALAKAGVNIEMINQGSSEVSLVFGIHSKDTKQAVRVLYEEFFGQKQ

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=473, Percent_Identity=30.8668076109937, Blast_Score=175, Evalue=7e-45,
Organism=Escherichia coli, GI1790455, Length=464, Percent_Identity=27.5862068965517, Blast_Score=123, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6320893, Length=495, Percent_Identity=24.040404040404, Blast_Score=110, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 49407; Mature: 49407

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVAKFGGSSVANAEQIKKVAAIIKADNKRKIVVVSAPGKRYADDAKMTDLLIALAEAKV
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHH
ADQPVEAAFNAVMNRYESIANDLELNRDVIAEIEADLKNRLEQTVTGKGRFLDIMKASGE
CCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
DNNAKLIAAYLSKIGLKATYINPKDAGLLVSDEPGNAQVLPEAYEALAKLAEIDGIVVFP
CCCHHHHHHHHHHHCCEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEEC
GFFGFSKLGNLVTFPRGGSDITGAILAAAVQAELYENFTDVDSVYAANPFIVEDPVEIKR
CCCCHHHHCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEEEECCHHHHH
MTYREMRELSYAGFSVFHDEALIPAFRNDIPVCVKNTNNPKCSGTLILAERERSLNPIIG
HHHHHHHHHHHCCHHHCCCCCCCHHHCCCCCEEEECCCCCCCCEEEEEEECCCCCCCEEE
IASDEGFATIYVRKYLMNREVGFGRHLLQILEDAGISYEHIPSGIDDTSVIIRQTQLSDQ
EECCCCCEEHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHCCCCCCCCHHEEEHHHHHHH
LENAIIERIKEELNVDDVHIEHDFAMVMLVGEGMHNTVGVTARASSALAKAGVNIEMINQ
HHHHHHHHHHHHCCCCEEEEECCEEEEEEECCCCCCCEEEEEHHHHHHHHCCCCEEEECC
GSSEVSLVFGIHSKDTKQAVRVLYEEFFGQKQ
CCCCEEEEEEECCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKVAKFGGSSVANAEQIKKVAAIIKADNKRKIVVVSAPGKRYADDAKMTDLLIALAEAKV
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHH
ADQPVEAAFNAVMNRYESIANDLELNRDVIAEIEADLKNRLEQTVTGKGRFLDIMKASGE
CCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
DNNAKLIAAYLSKIGLKATYINPKDAGLLVSDEPGNAQVLPEAYEALAKLAEIDGIVVFP
CCCHHHHHHHHHHHCCEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEEC
GFFGFSKLGNLVTFPRGGSDITGAILAAAVQAELYENFTDVDSVYAANPFIVEDPVEIKR
CCCCHHHHCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEEEECCHHHHH
MTYREMRELSYAGFSVFHDEALIPAFRNDIPVCVKNTNNPKCSGTLILAERERSLNPIIG
HHHHHHHHHHHCCHHHCCCCCCCHHHCCCCCEEEECCCCCCCCEEEEEEECCCCCCCEEE
IASDEGFATIYVRKYLMNREVGFGRHLLQILEDAGISYEHIPSGIDDTSVIIRQTQLSDQ
EECCCCCEEHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHCCCCCCCCHHEEEHHHHHHH
LENAIIERIKEELNVDDVHIEHDFAMVMLVGEGMHNTVGVTARASSALAKAGVNIEMINQ
HHHHHHHHHHHHCCCCEEEEECCEEEEEEECCCCCCCEEEEEHHHHHHHHCCCCEEEECC
GSSEVSLVFGIHSKDTKQAVRVLYEEFFGQKQ
CCCCEEEEEEECCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]