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The map label for this gene is thiDE [H]

Identifier: 56963502

GI number: 56963502

Start: 1863868

End: 1864686

Strand: Direct

Name: thiDE [H]

Synonym: ABC1737

Alternate gene names: 56963502

Gene position: 1863868-1864686 (Clockwise)

Preceding gene: 56963501

Following gene: 56963503

Centisome position: 43.31

GC content: 48.6

Gene sequence:

>819_bases
ATGCCCATTCCAACAGTCCTCTCCGTTGCTGGTTCTGATTCCAGCGGCGGTGCTGGCATTCAAGCAGACATAAAAACGTG
TCAAGAGCTTGGCGTTTATGCTGCTACTGCCATTACTGCTATTACAGCCCAAAATACAACTGGCGTACAATCGTGGCAAG
GACTTGACGCTTCACTCGTACGGGACCAAATCGAAGCAGTATTAAGTGATATCGGAGTGCATGTCATCAAGACAGGCATG
TTGGTCAATGCAGACATTGTCACAACGGTGGCTGAATTGGCAAATACGTATCGAGTAGCTAACATTGTCGTTGATCCCGT
CATGGCCTCGACAAGTGGAACAGCGCTTCTTTCGGCAGAAGGCCAACAGGTACTTGTACAGAAGCTTTTGCCACTTGCGA
CTGTTTTTACGCCTAATTTGCCTGAAACGGCCATGCTTCTTAATAAACCAGAATGTAAAACCGTTGCTGAAATGAAAGCA
GCCGCGGTACAATTGCATAAAATGGGGCCTAAGCTAGTCATTTTGAAAGGCGGCCATTTGCGACAAGGGGAAGCCATTGA
TTTATGCTACGATGGCCAAGCTTTCTTTGAGTTAAAGGCAAAACGGCTAGACAGAAAGCATACTCACGGGACTGGTTGTA
CGTTTGCTTCAGCGATAGCAGCAGAACTCGCAAAAGGGGCCAAGCCAATCGAAGCAGCTAAAAAAGCGAAAGCATATGTG
ACGGCCGCGATTTCGGAAGGAATCCCATTAGGAGCCGGAATCGGCCCGACGGATCATGCTGCTTATCGCCGCCTTGGCCA
AGAAGGGGGTTGCAATTGA

Upstream 100 bases:

>100_bases
CAAAAGTGGCGAGTGCGCAGTTGGCTGGGCAGAAGCCGCCTGTACCAATCGCCCTTTTTGATCCAGACCGTTTTTCGTAG
TCAGAGAAGGAGGCGTACGA

Downstream 100 bases:

>100_bases
GAAAGCATATAGTCGTATTAATATTGCCAGTCATTTTAATGATCGCGATCGCTTTGAGCGGTATTTATTACTTGAATCAA
GAAGAAACGATGACAAATGA

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Thiamine-phosphate pyrophosphorylase; TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase; Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MPIPTVLSVAGSDSSGGAGIQADIKTCQELGVYAATAITAITAQNTTGVQSWQGLDASLVRDQIEAVLSDIGVHVIKTGM
LVNADIVTTVAELANTYRVANIVVDPVMASTSGTALLSAEGQQVLVQKLLPLATVFTPNLPETAMLLNKPECKTVAEMKA
AAVQLHKMGPKLVILKGGHLRQGEAIDLCYDGQAFFELKAKRLDRKHTHGTGCTFASAIAAELAKGAKPIEAAKKAKAYV
TAAISEGIPLGAGIGPTDHAAYRRLGQEGGCN

Sequences:

>Translated_272_residues
MPIPTVLSVAGSDSSGGAGIQADIKTCQELGVYAATAITAITAQNTTGVQSWQGLDASLVRDQIEAVLSDIGVHVIKTGM
LVNADIVTTVAELANTYRVANIVVDPVMASTSGTALLSAEGQQVLVQKLLPLATVFTPNLPETAMLLNKPECKTVAEMKA
AAVQLHKMGPKLVILKGGHLRQGEAIDLCYDGQAFFELKAKRLDRKHTHGTGCTFASAIAAELAKGAKPIEAAKKAKAYV
TAAISEGIPLGAGIGPTDHAAYRRLGQEGGCN
>Mature_271_residues
PIPTVLSVAGSDSSGGAGIQADIKTCQELGVYAATAITAITAQNTTGVQSWQGLDASLVRDQIEAVLSDIGVHVIKTGML
VNADIVTTVAELANTYRVANIVVDPVMASTSGTALLSAEGQQVLVQKLLPLATVFTPNLPETAMLLNKPECKTVAEMKAA
AVQLHKMGPKLVILKGGHLRQGEAIDLCYDGQAFFELKAKRLDRKHTHGTGCTFASAIAAELAKGAKPIEAAKKAKAYVT
AAISEGIPLGAGIGPTDHAAYRRLGQEGGCN

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=255, Percent_Identity=40.7843137254902, Blast_Score=194, Evalue=5e-51,
Organism=Saccharomyces cerevisiae, GI6325378, Length=271, Percent_Identity=33.210332103321, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6324997, Length=271, Percent_Identity=36.5313653136531, Blast_Score=120, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6324517, Length=271, Percent_Identity=35.4243542435424, Blast_Score=116, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013749
- InterPro:   IPR004399
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF08543 Phos_pyr_kin; PF02581 TMP-TENI [H]

EC number: =2.5.1.3; =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 28198; Mature: 28067

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIPTVLSVAGSDSSGGAGIQADIKTCQELGVYAATAITAITAQNTTGVQSWQGLDASLV
CCCCCEEEECCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHEECCCCCCCHHHCCCCHHHH
RDQIEAVLSDIGVHVIKTGMLVNADIVTTVAELANTYRVANIVVDPVMASTSGTALLSAE
HHHHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHHHHHHHHHHEHHHHCCCCCCEEEECC
GQQVLVQKLLPLATVFTPNLPETAMLLNKPECKTVAEMKAAAVQLHKMGPKLVILKGGHL
CHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCC
RQGEAIDLCYDGQAFFELKAKRLDRKHTHGTGCTFASAIAAELAKGAKPIEAAKKAKAYV
CCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHH
TAAISEGIPLGAGIGPTDHAAYRRLGQEGGCN
HHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PIPTVLSVAGSDSSGGAGIQADIKTCQELGVYAATAITAITAQNTTGVQSWQGLDASLV
CCCCEEEECCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHEECCCCCCCHHHCCCCHHHH
RDQIEAVLSDIGVHVIKTGMLVNADIVTTVAELANTYRVANIVVDPVMASTSGTALLSAE
HHHHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHHHHHHHHHHEHHHHCCCCCCEEEECC
GQQVLVQKLLPLATVFTPNLPETAMLLNKPECKTVAEMKAAAVQLHKMGPKLVILKGGHL
CHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCC
RQGEAIDLCYDGQAFFELKAKRLDRKHTHGTGCTFASAIAAELAKGAKPIEAAKKAKAYV
CCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHH
TAAISEGIPLGAGIGPTDHAAYRRLGQEGGCN
HHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA