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The map label for this gene is sigA [H]

Identifier: 56963455

GI number: 56963455

Start: 1819087

End: 1820208

Strand: Direct

Name: sigA [H]

Synonym: ABC1690

Alternate gene names: 56963455

Gene position: 1819087-1820208 (Clockwise)

Preceding gene: 56963454

Following gene: 56963456

Centisome position: 42.27

GC content: 45.37

Gene sequence:

>1122_bases
ATGGCTGACAAACCGTTGCGTCCTGTAACAGAAGGTGACCTTACCATCGATCAAGTAAAAGAACAACTGGTGGAAACTGG
GAAAAAACGTGGTACATTGACGTATGCCGAAATTACAGAGAAAATGGCTGCGTTTGAACAAGATTCAGACCAAATGGATG
AATTTTACGAGTATCTTGGTGAACAAGGCGTGGAACTTTTGAACGAAAGTGAAGACGACGTGCCATCGCTTGCACAAGTA
GCCAAAGACGAAGAAGAGTTTGATTTAAATGATTTAAGCGTCCCTCCAGGGATTAAAATAAATGACCCAGTCCGTATGTA
TTTGAAAGAAATTGGCCGTGTTCCATTGCTTTCAGCGGAAGAAGAAATCGATTTGGCTAAACGAATTGAACAAGGCGACG
AAGAGGCGAAAAAACGGTTAGCGGAAGCGAATTTGCGCCTCGTTGTTTCGATTGCAAAACGTTATGTTGGCCGCGGCATG
CTATTCCTTGATTTAATTCAGGAAGGCAATATGGGTTTAATTAAAGCTGTTGAAAAGTTCGATTATGACAAAGGCTTTAA
ATTTAGCACATATGCCACTTGGTGGATCCGCCAAGCAATCACACGGGCCATTGCAGACCAAGCCCGTACGATCCGCATTC
CTGTGCATATGGTTGAAACGATTAATAAGCTGATCCGTGTACAGCGCCAACTTTTACAAGATTATGGGCGCGAGCCAACC
CCAGAAGAAGTCGGCAAAGAGATGGATTTAAGCCCCGACAAAGTAAGAGAGATTTTAAAAATTGCCCAAGAGCCTGTATC
GCTAGAGACGCCAATTGGCGAAGAGGATGATTCCCATCTAGGCGACTTTATCGAAGACCAAGAAGCTCTAGCGCCATCGG
ATGCAGCCGCGTACGAGCTGCTGAAAGAACAACTCGAAGACGTCCTCGATACACTAACTGACCGTGAGGAGAACGTATTG
CGCTTACGTTTCGGGCTTGATGACGGCCGGACAAGAACGCTTGAAGAAGTAGGCAAAGTGTTTGGCGTTACGAGAGAGCG
AATTCGCCAAATTGAAGCAAAAGCGCTCCGTAAATTGCGCCATCCTAGCCGCAGCAAGCGTTTAAAAGACTTTCTTGATT
AA

Upstream 100 bases:

>100_bases
ACCCAATCCGGGCGGCAAAACTGCAAATGGAGTTGATTCGCTTAAAAAAAGAGCTCCAAAGCCAGTAATGTATGGTTTAA
TTTTGGAAGGAGGGGATCGA

Downstream 100 bases:

>100_bases
TTAAAGAGATCGCTGATCGTTTGGCTTGCCCCTTTGCAAGCCAAACGATTCTATTTTGCTTAATTCCTATTTTACTGGGT
ATGAAAGGGAAATGCAAATC

Product: RNA polymerase sigma factor RpoD

Products: NA

Alternate protein names: Sigma-43; Sigma-A [H]

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MADKPLRPVTEGDLTIDQVKEQLVETGKKRGTLTYAEITEKMAAFEQDSDQMDEFYEYLGEQGVELLNESEDDVPSLAQV
AKDEEEFDLNDLSVPPGIKINDPVRMYLKEIGRVPLLSAEEEIDLAKRIEQGDEEAKKRLAEANLRLVVSIAKRYVGRGM
LFLDLIQEGNMGLIKAVEKFDYDKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVQRQLLQDYGREPT
PEEVGKEMDLSPDKVREILKIAQEPVSLETPIGEEDDSHLGDFIEDQEALAPSDAAAYELLKEQLEDVLDTLTDREENVL
RLRFGLDDGRTRTLEEVGKVFGVTRERIRQIEAKALRKLRHPSRSKRLKDFLD

Sequences:

>Translated_373_residues
MADKPLRPVTEGDLTIDQVKEQLVETGKKRGTLTYAEITEKMAAFEQDSDQMDEFYEYLGEQGVELLNESEDDVPSLAQV
AKDEEEFDLNDLSVPPGIKINDPVRMYLKEIGRVPLLSAEEEIDLAKRIEQGDEEAKKRLAEANLRLVVSIAKRYVGRGM
LFLDLIQEGNMGLIKAVEKFDYDKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVQRQLLQDYGREPT
PEEVGKEMDLSPDKVREILKIAQEPVSLETPIGEEDDSHLGDFIEDQEALAPSDAAAYELLKEQLEDVLDTLTDREENVL
RLRFGLDDGRTRTLEEVGKVFGVTRERIRQIEAKALRKLRHPSRSKRLKDFLD
>Mature_372_residues
ADKPLRPVTEGDLTIDQVKEQLVETGKKRGTLTYAEITEKMAAFEQDSDQMDEFYEYLGEQGVELLNESEDDVPSLAQVA
KDEEEFDLNDLSVPPGIKINDPVRMYLKEIGRVPLLSAEEEIDLAKRIEQGDEEAKKRLAEANLRLVVSIAKRYVGRGML
FLDLIQEGNMGLIKAVEKFDYDKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVQRQLLQDYGREPTP
EEVGKEMDLSPDKVREILKIAQEPVSLETPIGEEDDSHLGDFIEDQEALAPSDAAAYELLKEQLEDVLDTLTDREENVLR
LRFGLDDGRTRTLEEVGKVFGVTRERIRQIEAKALRKLRHPSRSKRLKDFLD

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=260, Percent_Identity=65, Blast_Score=345, Evalue=3e-96,
Organism=Escherichia coli, GI1789098, Length=302, Percent_Identity=43.7086092715232, Blast_Score=242, Evalue=2e-65,
Organism=Escherichia coli, GI1789871, Length=272, Percent_Identity=30.8823529411765, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1788231, Length=202, Percent_Identity=28.7128712871287, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 42705; Mature: 42574

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADKPLRPVTEGDLTIDQVKEQLVETGKKRGTLTYAEITEKMAAFEQDSDQMDEFYEYLG
CCCCCCCCCCCCCCCHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
EQGVELLNESEDDVPSLAQVAKDEEEFDLNDLSVPPGIKINDPVRMYLKEIGRVPLLSAE
HHHHHHHCCCCCCHHHHHHHHCCCHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCH
EEIDLAKRIEQGDEEAKKRLAEANLRLVVSIAKRYVGRGMLFLDLIQEGNMGLIKAVEKF
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHH
DYDKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVQRQLLQDYGREPT
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PEEVGKEMDLSPDKVREILKIAQEPVSLETPIGEEDDSHLGDFIEDQEALAPSDAAAYEL
HHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHH
LKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVGKVFGVTRERIRQIEAKALRKLR
HHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HPSRSKRLKDFLD
CCHHHHHHHHHCC
>Mature Secondary Structure 
ADKPLRPVTEGDLTIDQVKEQLVETGKKRGTLTYAEITEKMAAFEQDSDQMDEFYEYLG
CCCCCCCCCCCCCCHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
EQGVELLNESEDDVPSLAQVAKDEEEFDLNDLSVPPGIKINDPVRMYLKEIGRVPLLSAE
HHHHHHHCCCCCCHHHHHHHHCCCHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCH
EEIDLAKRIEQGDEEAKKRLAEANLRLVVSIAKRYVGRGMLFLDLIQEGNMGLIKAVEKF
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHH
DYDKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVQRQLLQDYGREPT
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PEEVGKEMDLSPDKVREILKIAQEPVSLETPIGEEDDSHLGDFIEDQEALAPSDAAAYEL
HHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHH
LKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVGKVFGVTRERIRQIEAKALRKLR
HHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HPSRSKRLKDFLD
CCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]