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The map label for this gene is dnaG [H]

Identifier: 56963454

GI number: 56963454

Start: 1817243

End: 1819054

Strand: Direct

Name: dnaG [H]

Synonym: ABC1689

Alternate gene names: 56963454

Gene position: 1817243-1819054 (Clockwise)

Preceding gene: 56963453

Following gene: 56963455

Centisome position: 42.22

GC content: 47.19

Gene sequence:

>1812_bases
GTGGCTGTGAAAATACCCGATGAAAAACTTACAGAGGTCAGGGAAGCAAGTGATATTGTTGAGGTCATCGGCGAATATGT
GCAGCTAAAAAAGCAAGGGCGTCAGTACGGAGGCCTTTGCCCGTTTCATGACGAGAAAACGCCGTCTTTTTCTGTATCGG
AAGACAAACAAGTGTACCATTGTTTTGGCTGTGGAGCCGGTGGGAATGTGTTTACTTTTTTGCAGGAATTGAAGGGCTGG
TCTTTTTCTGAAACCGTCTCCCACCTCGCAGAACGTGCAAAAATAGCTTTGCCGCAAATGCAAAAAACAGAGACGGACAA
ACCTGAAGATGCGCGCATCAGGCAAATGAAAAAAGGCCACGAGCTCGCTGCCTCTACTTACCATAGCATTTTGACGCTAA
CAGAAGAAGGGGCTCCAGGGCGTGTTTATGCACAAGAGAGGCTTTTCACGCAAGACCAGATTGAACATTTCCGAATCGGC
TATGCCCTTAATGATTGGGAAACGCTCGCTGTGATCTTTGAAACAAATGGCCTTGACTTAGCTGTAATGGCAGAATCAGG
CCTTTTGGCGAAACGCAAACAAGGAACGGGTTATTATGACTTGTTTCGCGAGCGACTTATGTTTCCGATTACGGATAGTC
AAGGAAGCGTAGTGGCATTTGGCGGTAGGGCCATTGGCGAAGGCCAACCGAAATACTTGAACAGCCCGGAAACGCCGATT
TTCCACAAAGCAAAGACGTTATTCCATATGCATGAAGCTCGTCCGAGCATCCGTAAACAAAACAAAGCCCTGTTAGTAGA
AGGGGCATTTGACGTAATGGCCGCTTGGAAAGCTGGAGTGACAAATGTTGTTGCTACGCTAGGCACTGCTTTAAGCAGCG
ATCATGCCAAACTTCTTCGCCGCAATGCAGAAGAAGTCATTTTATGTTATGATGGCGACCGTGCCGGGAGAGAAGCGATT
CGCAAAGCGATTCCCGTGTTGGAGGAAGCTGGCTGCAAAGTGTCGATTTGCCTACTTCCCGAAGGGCTCGACCCAGATGA
TTATGTGAAGCAGCATGGCGAAGAGGCATTTGTGCGCATACTCGACAACGAATGTTTATCCGTAATGGCCTTTCGAATGT
TGGATTTAAAGCAAGGCAAGAATATGCAAAATGAAGGAGATCGGCTCCAATATATCGAAGAAATCGTACAGATGTTGGCA
AAACTCGAGCATTCTGTCGAACAAGAATACTATTTGCAGCAACTTGCAGAAAGTTTTACACTGCCGATGGATGTGTTGCG
CCAAGAGTTAAGCCGGGCCGAACGCCTACTCAAACAAACAAAAGAGACACCTAAAGCGCTGCCGCGAGCGGAAAAGCCAA
CTTCCGGCCGAAAGCAAGGAAGCGCACGGCCTGTACCAGCTCATGTCAAAGCCGAACGGATCTTGCTAGCTTATATGCTC
CAAGATACGGAAATGGTGTGGCGCGTCTCAGAAAAGCTAGGAACTGGCTTTAACCTCGAAGAACACCAAGCCCTTTATGC
TTATTTGCTTTCATTTGTAACTAGAGAGCCTGATGCGAGCATGAAGCGTTTTGTAGAAAGCCTTGAAGATCGAAGGCTTG
CAAAGCTGGCGGCATATTTGCTCATGCAACCATTAAAAAATGAGTGTTCAGACGAGGAGCTTGCTGATTATATAAAGCGG
ATTGAGCAATACCCAAAATGGGTAAAACTGAAAGAACAGCAGCAGGCTGCGCGGTTTGAACAAGACCCAATCCGGGCGGC
AAAACTGCAAATGGAGTTGATTCGCTTAAAAAAAGAGCTCCAAAGCCAGTAA

Upstream 100 bases:

>100_bases
CCCAAAAAAATTTACAGCATCTGACCGAGCCGCGTTCTATCATGCTCTATATGCTGTACTTGAAAAGATAGCAAAGGAAT
AGAATTGTGGTGATTGCCTA

Downstream 100 bases:

>100_bases
TGTATGGTTTAATTTTGGAAGGAGGGGATCGAATGGCTGACAAACCGTTGCGTCCTGTAACAGAAGGTGACCTTACCATC
GATCAAGTAAAAGAACAACT

Product: DNA primase DnaG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 603; Mature: 602

Protein sequence:

>603_residues
MAVKIPDEKLTEVREASDIVEVIGEYVQLKKQGRQYGGLCPFHDEKTPSFSVSEDKQVYHCFGCGAGGNVFTFLQELKGW
SFSETVSHLAERAKIALPQMQKTETDKPEDARIRQMKKGHELAASTYHSILTLTEEGAPGRVYAQERLFTQDQIEHFRIG
YALNDWETLAVIFETNGLDLAVMAESGLLAKRKQGTGYYDLFRERLMFPITDSQGSVVAFGGRAIGEGQPKYLNSPETPI
FHKAKTLFHMHEARPSIRKQNKALLVEGAFDVMAAWKAGVTNVVATLGTALSSDHAKLLRRNAEEVILCYDGDRAGREAI
RKAIPVLEEAGCKVSICLLPEGLDPDDYVKQHGEEAFVRILDNECLSVMAFRMLDLKQGKNMQNEGDRLQYIEEIVQMLA
KLEHSVEQEYYLQQLAESFTLPMDVLRQELSRAERLLKQTKETPKALPRAEKPTSGRKQGSARPVPAHVKAERILLAYML
QDTEMVWRVSEKLGTGFNLEEHQALYAYLLSFVTREPDASMKRFVESLEDRRLAKLAAYLLMQPLKNECSDEELADYIKR
IEQYPKWVKLKEQQQAARFEQDPIRAAKLQMELIRLKKELQSQ

Sequences:

>Translated_603_residues
MAVKIPDEKLTEVREASDIVEVIGEYVQLKKQGRQYGGLCPFHDEKTPSFSVSEDKQVYHCFGCGAGGNVFTFLQELKGW
SFSETVSHLAERAKIALPQMQKTETDKPEDARIRQMKKGHELAASTYHSILTLTEEGAPGRVYAQERLFTQDQIEHFRIG
YALNDWETLAVIFETNGLDLAVMAESGLLAKRKQGTGYYDLFRERLMFPITDSQGSVVAFGGRAIGEGQPKYLNSPETPI
FHKAKTLFHMHEARPSIRKQNKALLVEGAFDVMAAWKAGVTNVVATLGTALSSDHAKLLRRNAEEVILCYDGDRAGREAI
RKAIPVLEEAGCKVSICLLPEGLDPDDYVKQHGEEAFVRILDNECLSVMAFRMLDLKQGKNMQNEGDRLQYIEEIVQMLA
KLEHSVEQEYYLQQLAESFTLPMDVLRQELSRAERLLKQTKETPKALPRAEKPTSGRKQGSARPVPAHVKAERILLAYML
QDTEMVWRVSEKLGTGFNLEEHQALYAYLLSFVTREPDASMKRFVESLEDRRLAKLAAYLLMQPLKNECSDEELADYIKR
IEQYPKWVKLKEQQQAARFEQDPIRAAKLQMELIRLKKELQSQ
>Mature_602_residues
AVKIPDEKLTEVREASDIVEVIGEYVQLKKQGRQYGGLCPFHDEKTPSFSVSEDKQVYHCFGCGAGGNVFTFLQELKGWS
FSETVSHLAERAKIALPQMQKTETDKPEDARIRQMKKGHELAASTYHSILTLTEEGAPGRVYAQERLFTQDQIEHFRIGY
ALNDWETLAVIFETNGLDLAVMAESGLLAKRKQGTGYYDLFRERLMFPITDSQGSVVAFGGRAIGEGQPKYLNSPETPIF
HKAKTLFHMHEARPSIRKQNKALLVEGAFDVMAAWKAGVTNVVATLGTALSSDHAKLLRRNAEEVILCYDGDRAGREAIR
KAIPVLEEAGCKVSICLLPEGLDPDDYVKQHGEEAFVRILDNECLSVMAFRMLDLKQGKNMQNEGDRLQYIEEIVQMLAK
LEHSVEQEYYLQQLAESFTLPMDVLRQELSRAERLLKQTKETPKALPRAEKPTSGRKQGSARPVPAHVKAERILLAYMLQ
DTEMVWRVSEKLGTGFNLEEHQALYAYLLSFVTREPDASMKRFVESLEDRRLAKLAAYLLMQPLKNECSDEELADYIKRI
EQYPKWVKLKEQQQAARFEQDPIRAAKLQMELIRLKKELQSQ

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=568, Percent_Identity=31.8661971830986, Blast_Score=280, Evalue=2e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007693
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 68538; Mature: 68407

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVKIPDEKLTEVREASDIVEVIGEYVQLKKQGRQYGGLCPFHDEKTPSFSVSEDKQVYH
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCCCCCCCCCCCCCCCCCCCCCEEE
CFGCGAGGNVFTFLQELKGWSFSETVSHLAERAKIALPQMQKTETDKPEDARIRQMKKGH
EEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHH
ELAASTYHSILTLTEEGAPGRVYAQERLFTQDQIEHFRIGYALNDWETLAVIFETNGLDL
HHHHHHHHHHHEEECCCCCCCEEHHHHHHHHHHHHHHHCCEEECCCEEEEEEEECCCCEE
AVMAESGLLAKRKQGTGYYDLFRERLMFPITDSQGSVVAFGGRAIGEGQPKYLNSPETPI
EEEECCCCHHHHCCCCCHHHHHHHHHCCCEECCCCCEEEECCEECCCCCCCCCCCCCCCH
FHKAKTLFHMHEARPSIRKQNKALLVEGAFDVMAAWKAGVTNVVATLGTALSSDHAKLLR
HHHHHHHHHHHHHCHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
RNAEEVILCYDGDRAGREAIRKAIPVLEEAGCKVSICLLPEGLDPDDYVKQHGEEAFVRI
CCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHCCHHHHHHH
LDNECLSVMAFRMLDLKQGKNMQNEGDRLQYIEEIVQMLAKLEHSVEQEYYLQQLAESFT
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LPMDVLRQELSRAERLLKQTKETPKALPRAEKPTSGRKQGSARPVPAHVKAERILLAYML
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
QDTEMVWRVSEKLGTGFNLEEHQALYAYLLSFVTREPDASMKRFVESLEDRRLAKLAAYL
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
LMQPLKNECSDEELADYIKRIEQYPKWVKLKEQQQAARFEQDPIRAAKLQMELIRLKKEL
HHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
QSQ
CCC
>Mature Secondary Structure 
AVKIPDEKLTEVREASDIVEVIGEYVQLKKQGRQYGGLCPFHDEKTPSFSVSEDKQVYH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCCCCCCCCCCCCCCCCCCCCCEEE
CFGCGAGGNVFTFLQELKGWSFSETVSHLAERAKIALPQMQKTETDKPEDARIRQMKKGH
EEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHH
ELAASTYHSILTLTEEGAPGRVYAQERLFTQDQIEHFRIGYALNDWETLAVIFETNGLDL
HHHHHHHHHHHEEECCCCCCCEEHHHHHHHHHHHHHHHCCEEECCCEEEEEEEECCCCEE
AVMAESGLLAKRKQGTGYYDLFRERLMFPITDSQGSVVAFGGRAIGEGQPKYLNSPETPI
EEEECCCCHHHHCCCCCHHHHHHHHHCCCEECCCCCEEEECCEECCCCCCCCCCCCCCCH
FHKAKTLFHMHEARPSIRKQNKALLVEGAFDVMAAWKAGVTNVVATLGTALSSDHAKLLR
HHHHHHHHHHHHHCHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
RNAEEVILCYDGDRAGREAIRKAIPVLEEAGCKVSICLLPEGLDPDDYVKQHGEEAFVRI
CCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHCCHHHHHHH
LDNECLSVMAFRMLDLKQGKNMQNEGDRLQYIEEIVQMLAKLEHSVEQEYYLQQLAESFT
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LPMDVLRQELSRAERLLKQTKETPKALPRAEKPTSGRKQGSARPVPAHVKAERILLAYML
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
QDTEMVWRVSEKLGTGFNLEEHQALYAYLLSFVTREPDASMKRFVESLEDRRLAKLAAYL
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
LMQPLKNECSDEELADYIKRIEQYPKWVKLKEQQQAARFEQDPIRAAKLQMELIRLKKEL
HHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
QSQ
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]