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The map label for this gene is glyS

Identifier: 56963450

GI number: 56963450

Start: 1812908

End: 1814989

Strand: Direct

Name: glyS

Synonym: ABC1685

Alternate gene names: 56963450

Gene position: 1812908-1814989 (Clockwise)

Preceding gene: 56963449

Following gene: 56963451

Centisome position: 42.12

GC content: 46.45

Gene sequence:

>2082_bases
ATGAATAAGCCATTTTTACTTGAACTAGGATTGGAGGAGTTACCGGCTCGTTTTGTTACGCCTTCCATTACACAGCTCGC
TGAAAAAGTGCAAGCATGGCTTGATGATAAAGGCCTTTCGTACGGAGAAATCCACTCTTACGCTACGCCAAGAAGGCTGG
CTATACTCGTTGAAAATTTGGCGGACCGGCAGCCTGATATACATGATGAATCGAGAGGGCCTGCCTTAAAAATTGCCAAA
GACGAAAGCGGCGCTTGGACAAAAGCGGCGCTTGGATTTGCGAAAGGACAAGGAGTAGAAACGAGCCAGCTGGAAATAAA
AGCGGTAAATGGCACTGATTATGTGTTTGCCAAAACACATAAAAAGGGTGAAAAAACAAGCGTGCTATTGCCGGAACTTA
AAGAATTGATTACGGGCTTAACATTTCCAAAAAGCATGCGTTGGAACACCTACTCCCTTCGCTATGCTCGGCCGATCCAG
TGGCTTGTCGCTCTCTATGGCGAGGACGTCATTCCTTTTTCAATTAACGGTATCGAAACAGGTCGAAACACGACTGGACA
CCGTTTTTTAGGGGCTGACTTTCCATTAGACAATCCAATCGAGTATGCGGACGCCCTGAAACGTCAATATGTCATTGCCG
ATGCTGAAGAGCGAAAAGCAGCGATTGTAAGTCAGTTAAAGCAAATGGAGGAAGAGCATAGCTGGGTTGTGCCAATAGAT
GAAGCGCTTCTTGAAGAAGTGACACAATTAGTCGAGTATCCAACTGCGCTATCAGGCTCATTTGACGATTCGTTTTTAGC
TTTGCCGAAAGAAGTATTAATTACAACGATGCGTGAACATCAGCGTTATTTTCCAGTAGAAGCGAAAGATGGCCGTTTGC
TTCCGTACTTTATTACAGTGCGAAACGGAAATGAAGAATATATTGAAAATGTCGCCAAGGGCAATGAAAAAGTGTTGCGT
GCTCGCTTGTCTGATGCTGCCTTTTTTTATCATGAAGACCAAAAGATTTCTCTTCAGGCAGCAAACGCTAAACTAGACAA
CATCGTTTTTCATGAAGCGATTGGTACAATGGGCGAAAAGGTAGCCCGTATCGGCGAATTGTCCAAATGGCTTGCAGGTG
AGGCGAACTTGGGTGCCCAAGAAAGGCAAACAGTGGAGCGTGCTGCCGCTATTGCCAAGTTCGACCTTGTGACTTACATG
GTTGGCGAATTCCCAGAACTACAAGGAAGAATGGGTCAGGAATATGCGGAAAAAGCAGGAGAGACCCCTCAAGTAGCGAA
AGCGATTTATGAGCAGTACTTGCCTCGCTATGCAGGGGACAAAGTGCCTGAAACTGAAGCGGGTGCAGTGTTGGCGCTTG
CCGACAAACTCGATACGGTCGTCTCTTGTTTTTCCATTGGCTTAATCCCCACTGGCTCACAAGATCCGTATGCGTTGCGC
CGCCAAGCAACGGGGATCATCCACATTCTTCTCCAAACTGAAATGCCTTTGACACTTGAAAACATGGCTGTTCATGCGCT
TGATTCCATTGCAAAAAAAGGGTTTATGACAGAAAAAGTCGAAACGACCAAGCAGGAATTAATCAGCTTTTTTGCGAATC
GCTTGAAGCATGTCATGCTAGAAGCTGGCTTTCGCTATGATATTGTTGATGCGGTGCTTGCCGCTCCGCTTGTCGATGTT
TATACGATGTTCGCCAAGGCAAGGCTGTTAACTGAACGTGCGGAAGACCGGGAGTTTAAAGAAGTGACCGAAAGCTTGAG
TCGTGTCACAAATTTGGCTAAAAAAGCGCCAAAAGGTGTTTTCGTTTCCGAAAATCTGTTTGAAAATGAAACGGAGTCAC
GCTTGCTTGCTGCTACGCTAGAGGTCGAGTTCGGCTTGGCTGAGGCTTGGAAGGTCAAAGATGCGTATGCCGCTTATCAA
GCGTTGGCTCAGTGTCGTGCCACGATCAACACGTTCTTTGACCATACGATGGTGATGGCTGACAATGAAGCCACTCGCAA
TAATCGCCTTGCGCTGCTTGACCGATTGGCGAAATCAATCCAGTCCTATGCTGATTTTCAAAAGATCGTCTTCTCGGCCT
AA

Upstream 100 bases:

>100_bases
ATTGCCCGGGTTCGCACACTCGCTCGAAAAGCGGCGCGCAAGTACTATGAAGAGAGAGAAAAGCTTGGCTTTCCTATGCT
TAAAAAGGAGGACGCCCACG

Downstream 100 bases:

>100_bases
GATTATTGGCACTGGCTTAAAAAAGAGGTGAGTCCAATCGAATTATCAACTAGGCAACAGCAAATCGTCGATATTGTAAA
GGAAAATGGCCCAATTACCG

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS

Number of amino acids: Translated: 693; Mature: 693

Protein sequence:

>693_residues
MNKPFLLELGLEELPARFVTPSITQLAEKVQAWLDDKGLSYGEIHSYATPRRLAILVENLADRQPDIHDESRGPALKIAK
DESGAWTKAALGFAKGQGVETSQLEIKAVNGTDYVFAKTHKKGEKTSVLLPELKELITGLTFPKSMRWNTYSLRYARPIQ
WLVALYGEDVIPFSINGIETGRNTTGHRFLGADFPLDNPIEYADALKRQYVIADAEERKAAIVSQLKQMEEEHSWVVPID
EALLEEVTQLVEYPTALSGSFDDSFLALPKEVLITTMREHQRYFPVEAKDGRLLPYFITVRNGNEEYIENVAKGNEKVLR
ARLSDAAFFYHEDQKISLQAANAKLDNIVFHEAIGTMGEKVARIGELSKWLAGEANLGAQERQTVERAAAIAKFDLVTYM
VGEFPELQGRMGQEYAEKAGETPQVAKAIYEQYLPRYAGDKVPETEAGAVLALADKLDTVVSCFSIGLIPTGSQDPYALR
RQATGIIHILLQTEMPLTLENMAVHALDSIAKKGFMTEKVETTKQELISFFANRLKHVMLEAGFRYDIVDAVLAAPLVDV
YTMFAKARLLTERAEDREFKEVTESLSRVTNLAKKAPKGVFVSENLFENETESRLLAATLEVEFGLAEAWKVKDAYAAYQ
ALAQCRATINTFFDHTMVMADNEATRNNRLALLDRLAKSIQSYADFQKIVFSA

Sequences:

>Translated_693_residues
MNKPFLLELGLEELPARFVTPSITQLAEKVQAWLDDKGLSYGEIHSYATPRRLAILVENLADRQPDIHDESRGPALKIAK
DESGAWTKAALGFAKGQGVETSQLEIKAVNGTDYVFAKTHKKGEKTSVLLPELKELITGLTFPKSMRWNTYSLRYARPIQ
WLVALYGEDVIPFSINGIETGRNTTGHRFLGADFPLDNPIEYADALKRQYVIADAEERKAAIVSQLKQMEEEHSWVVPID
EALLEEVTQLVEYPTALSGSFDDSFLALPKEVLITTMREHQRYFPVEAKDGRLLPYFITVRNGNEEYIENVAKGNEKVLR
ARLSDAAFFYHEDQKISLQAANAKLDNIVFHEAIGTMGEKVARIGELSKWLAGEANLGAQERQTVERAAAIAKFDLVTYM
VGEFPELQGRMGQEYAEKAGETPQVAKAIYEQYLPRYAGDKVPETEAGAVLALADKLDTVVSCFSIGLIPTGSQDPYALR
RQATGIIHILLQTEMPLTLENMAVHALDSIAKKGFMTEKVETTKQELISFFANRLKHVMLEAGFRYDIVDAVLAAPLVDV
YTMFAKARLLTERAEDREFKEVTESLSRVTNLAKKAPKGVFVSENLFENETESRLLAATLEVEFGLAEAWKVKDAYAAYQ
ALAQCRATINTFFDHTMVMADNEATRNNRLALLDRLAKSIQSYADFQKIVFSA
>Mature_693_residues
MNKPFLLELGLEELPARFVTPSITQLAEKVQAWLDDKGLSYGEIHSYATPRRLAILVENLADRQPDIHDESRGPALKIAK
DESGAWTKAALGFAKGQGVETSQLEIKAVNGTDYVFAKTHKKGEKTSVLLPELKELITGLTFPKSMRWNTYSLRYARPIQ
WLVALYGEDVIPFSINGIETGRNTTGHRFLGADFPLDNPIEYADALKRQYVIADAEERKAAIVSQLKQMEEEHSWVVPID
EALLEEVTQLVEYPTALSGSFDDSFLALPKEVLITTMREHQRYFPVEAKDGRLLPYFITVRNGNEEYIENVAKGNEKVLR
ARLSDAAFFYHEDQKISLQAANAKLDNIVFHEAIGTMGEKVARIGELSKWLAGEANLGAQERQTVERAAAIAKFDLVTYM
VGEFPELQGRMGQEYAEKAGETPQVAKAIYEQYLPRYAGDKVPETEAGAVLALADKLDTVVSCFSIGLIPTGSQDPYALR
RQATGIIHILLQTEMPLTLENMAVHALDSIAKKGFMTEKVETTKQELISFFANRLKHVMLEAGFRYDIVDAVLAAPLVDV
YTMFAKARLLTERAEDREFKEVTESLSRVTNLAKKAPKGVFVSENLFENETESRLLAATLEVEFGLAEAWKVKDAYAAYQ
ALAQCRATINTFFDHTMVMADNEATRNNRLALLDRLAKSIQSYADFQKIVFSA

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1789982, Length=697, Percent_Identity=35.8680057388809, Blast_Score=410, Evalue=1e-115,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYGB_BACSK (Q5WHD5)

Other databases:

- EMBL:   AP006627
- RefSeq:   YP_175181.1
- ProteinModelPortal:   Q5WHD5
- STRING:   Q5WHD5
- EnsemblBacteria:   EBBACT00000049371
- GeneID:   3204539
- GenomeReviews:   AP006627_GR
- KEGG:   bcl:ABC1685
- NMPDR:   fig|66692.3.peg.68
- eggNOG:   COG0751
- GeneTree:   EBGT00050000002728
- HOGENOM:   HBG586599
- OMA:   FIRPVHT
- ProtClustDB:   PRK01233
- BioCyc:   BCLA66692:ABC1685-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00255
- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311
- PRINTS:   PR01045
- TIGRFAMs:   TIGR00211

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f

EC number: =6.1.1.14

Molecular weight: Translated: 77517; Mature: 77517

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKPFLLELGLEELPARFVTPSITQLAEKVQAWLDDKGLSYGEIHSYATPRRLAILVENL
CCCCEEEECCHHHCCHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHCCCCCHHHHHHHHHH
ADRQPDIHDESRGPALKIAKDESGAWTKAALGFAKGQGVETSQLEIKAVNGTDYVFAKTH
HCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEECC
KKGEKTSVLLPELKELITGLTFPKSMRWNTYSLRYARPIQWLVALYGEDVIPFSINGIET
CCCCCCEEHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCCEEEECCCCC
GRNTTGHRFLGADFPLDNPIEYADALKRQYVIADAEERKAAIVSQLKQMEEEHSWVVPID
CCCCCCCEEECCCCCCCCCHHHHHHHHHHEEEECCHHHHHHHHHHHHHHHHHCCEEEEHH
EALLEEVTQLVEYPTALSGSFDDSFLALPKEVLITTMREHQRYFPVEAKDGRLLPYFITV
HHHHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEE
RNGNEEYIENVAKGNEKVLRARLSDAAFFYHEDQKISLQAANAKLDNIVFHEAIGTMGEK
ECCCHHHHHHHHCCHHHHHHHHHHHHEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHH
VARIGELSKWLAGEANLGAQERQTVERAAAIAKFDLVTYMVGEFPELQGRMGQEYAEKAG
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHCC
ETPQVAKAIYEQYLPRYAGDKVPETEAGAVLALADKLDTVVSCFSIGLIPTGSQDPYALR
CCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCHHHHH
RQATGIIHILLQTEMPLTLENMAVHALDSIAKKGFMTEKVETTKQELISFFANRLKHVML
HHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EAGFRYDIVDAVLAAPLVDVYTMFAKARLLTERAEDREFKEVTESLSRVTNLAKKAPKGV
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCE
FVSENLFENETESRLLAATLEVEFGLAEAWKVKDAYAAYQALAQCRATINTFFDHTMVMA
EECCHHHCCCHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEE
DNEATRNNRLALLDRLAKSIQSYADFQKIVFSA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNKPFLLELGLEELPARFVTPSITQLAEKVQAWLDDKGLSYGEIHSYATPRRLAILVENL
CCCCEEEECCHHHCCHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHCCCCCHHHHHHHHHH
ADRQPDIHDESRGPALKIAKDESGAWTKAALGFAKGQGVETSQLEIKAVNGTDYVFAKTH
HCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEECC
KKGEKTSVLLPELKELITGLTFPKSMRWNTYSLRYARPIQWLVALYGEDVIPFSINGIET
CCCCCCEEHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCCEEEECCCCC
GRNTTGHRFLGADFPLDNPIEYADALKRQYVIADAEERKAAIVSQLKQMEEEHSWVVPID
CCCCCCCEEECCCCCCCCCHHHHHHHHHHEEEECCHHHHHHHHHHHHHHHHHCCEEEEHH
EALLEEVTQLVEYPTALSGSFDDSFLALPKEVLITTMREHQRYFPVEAKDGRLLPYFITV
HHHHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEE
RNGNEEYIENVAKGNEKVLRARLSDAAFFYHEDQKISLQAANAKLDNIVFHEAIGTMGEK
ECCCHHHHHHHHCCHHHHHHHHHHHHEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHH
VARIGELSKWLAGEANLGAQERQTVERAAAIAKFDLVTYMVGEFPELQGRMGQEYAEKAG
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHCC
ETPQVAKAIYEQYLPRYAGDKVPETEAGAVLALADKLDTVVSCFSIGLIPTGSQDPYALR
CCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCHHHHH
RQATGIIHILLQTEMPLTLENMAVHALDSIAKKGFMTEKVETTKQELISFFANRLKHVML
HHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EAGFRYDIVDAVLAAPLVDVYTMFAKARLLTERAEDREFKEVTESLSRVTNLAKKAPKGV
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCE
FVSENLFENETESRLLAATLEVEFGLAEAWKVKDAYAAYQALAQCRATINTFFDHTMVMA
EECCHHHCCCHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEE
DNEATRNNRLALLDRLAKSIQSYADFQKIVFSA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA