Click here to switch to the map view.

The map label for this gene is grpE

Identifier: 56963423

GI number: 56963423

Start: 1787608

End: 1788186

Strand: Direct

Name: grpE

Synonym: ABC1658

Alternate gene names: 56963423

Gene position: 1787608-1788186 (Clockwise)

Preceding gene: 56963422

Following gene: 56963424

Centisome position: 41.53

GC content: 45.42

Gene sequence:

>579_bases
GTGGCAGAAGAGAAGAACGAGGAACTGCAAACAGAAGAAACAGAACAAACAAAAGAAGACGGCCACGGCGATTCCGCTGA
GCAAGAAGAGACTGAAAACAATAGCGGAACTGAAGTTGAAGAACAAGAAGAGCCAGAGGTTCACCCTCTTGAAATAGAGC
TAAATGAATTGAAAGACCGTCTCGCTCGCGTTCGTGCTGATTATGAAAATTTTCGCCGTCGCACAAAAGAGGAGAAAGAG
GCGCAGGCAAAATACCGCGCCCAAGGCTTTATTGAAAAACTGCTCCCTGCGCTTGATAACTTCGAACGCGCTTTGTTGGT
CGAACCTAAACATGAAGAAGCAAAGCAATTGCTCCAAGGAATGGAAATGGTGTACCGCCAAGTAGAAGAAGCATTAAAAC
AAGAAGGGGTAGAACCGATTCCTACAGAAGGTGAGCTGTTCGACCCACATCTCCACCAAGCAGTGATGCAAGTGTCAGAG
GAAGGCTATGAGCCGAACCAAATTGTTGAGGAACTTCAAAAAGGGTACAAACTAAAAGACCGAGTGATTCGACACTCAAT
GGTGAAAGTAAATCAATAG

Upstream 100 bases:

>100_bases
TCGTATACGCAGCAGTCCTCTGCTGCTATACATAGAAGGCGCGGGCAAATGTCCAAAGCCCGCAAGCTATAGACGATGAT
TTTACAGGAGGTGAACAAAC

Downstream 100 bases:

>100_bases
GACTTGGAGGAACGACGCATGAGCAAAATTATTGGTATTGACTTAGGGACAACAAACTCTTGTGTGGCCGTAATGGAGGG
CGGCGAAGCCACGGTTATCC

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 192; Mature: 191

Protein sequence:

>192_residues
MAEEKNEELQTEETEQTKEDGHGDSAEQEETENNSGTEVEEQEEPEVHPLEIELNELKDRLARVRADYENFRRRTKEEKE
AQAKYRAQGFIEKLLPALDNFERALLVEPKHEEAKQLLQGMEMVYRQVEEALKQEGVEPIPTEGELFDPHLHQAVMQVSE
EGYEPNQIVEELQKGYKLKDRVIRHSMVKVNQ

Sequences:

>Translated_192_residues
MAEEKNEELQTEETEQTKEDGHGDSAEQEETENNSGTEVEEQEEPEVHPLEIELNELKDRLARVRADYENFRRRTKEEKE
AQAKYRAQGFIEKLLPALDNFERALLVEPKHEEAKQLLQGMEMVYRQVEEALKQEGVEPIPTEGELFDPHLHQAVMQVSE
EGYEPNQIVEELQKGYKLKDRVIRHSMVKVNQ
>Mature_191_residues
AEEKNEELQTEETEQTKEDGHGDSAEQEETENNSGTEVEEQEEPEVHPLEIELNELKDRLARVRADYENFRRRTKEEKEA
QAKYRAQGFIEKLLPALDNFERALLVEPKHEEAKQLLQGMEMVYRQVEEALKQEGVEPIPTEGELFDPHLHQAVMQVSEE
GYEPNQIVEELQKGYKLKDRVIRHSMVKVNQ

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=147, Percent_Identity=31.9727891156463, Blast_Score=71, Evalue=6e-13,
Organism=Escherichia coli, GI1788967, Length=134, Percent_Identity=35.0746268656716, Blast_Score=94, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6324806, Length=181, Percent_Identity=33.1491712707182, Blast_Score=87, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24653432, Length=162, Percent_Identity=32.0987654320988, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_BACSK (Q5WHG2)

Other databases:

- EMBL:   AP006627
- RefSeq:   YP_175154.1
- ProteinModelPortal:   Q5WHG2
- SMR:   Q5WHG2
- STRING:   Q5WHG2
- EnsemblBacteria:   EBBACT00000049146
- GeneID:   3204549
- GenomeReviews:   AP006627_GR
- KEGG:   bcl:ABC1658
- NMPDR:   fig|66692.3.peg.41
- eggNOG:   COG0576
- GeneTree:   EBGT00050000001213
- HOGENOM:   HBG732630
- OMA:   NLHQAVM
- ProtClustDB:   PRK14140
- BioCyc:   BCLA66692:ABC1658-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 22455; Mature: 22324

Theoretical pI: Translated: 4.31; Mature: 4.31

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEEKNEELQTEETEQTKEDGHGDSAEQEETENNSGTEVEEQEEPEVHPLEIELNELKDR
CCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCHHEEHHHHHHH
LARVRADYENFRRRTKEEKEAQAKYRAQGFIEKLLPALDNFERALLVEPKHEEAKQLLQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHH
MEMVYRQVEEALKQEGVEPIPTEGELFDPHLHQAVMQVSEEGYEPNQIVEELQKGYKLKD
HHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHH
RVIRHSMVKVNQ
HHHHHHHHHCCC
>Mature Secondary Structure 
AEEKNEELQTEETEQTKEDGHGDSAEQEETENNSGTEVEEQEEPEVHPLEIELNELKDR
CCCCCHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCHHEEHHHHHHH
LARVRADYENFRRRTKEEKEAQAKYRAQGFIEKLLPALDNFERALLVEPKHEEAKQLLQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHH
MEMVYRQVEEALKQEGVEPIPTEGELFDPHLHQAVMQVSEEGYEPNQIVEELQKGYKLKD
HHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHH
RVIRHSMVKVNQ
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA