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The map label for this gene is nasD [H]

Identifier: 56963386

GI number: 56963386

Start: 1748380

End: 1750821

Strand: Direct

Name: nasD [H]

Synonym: ABC1621

Alternate gene names: 56963386

Gene position: 1748380-1750821 (Clockwise)

Preceding gene: 56963385

Following gene: 56963387

Centisome position: 40.62

GC content: 45.37

Gene sequence:

>2442_bases
GTGAATAAAGAAAAGGTTGTTTTAATTGGCAATGGCATGGCGGGCATCCGCACACTCGAAGAAATCATTAAACGAGAGCC
TAGCCGTTTTCAATTCACTGTTTTCGGTGCAGAACCCCATCCAAATTACAATCGAATATTGTTGTCGTCTGTTCTTCAAG
GCGACGCAAGTGTAAGCGACATCGTCTTAAACGACTACGACTGGTATGAAAGAAACCAAATTGAATTGTTTACAGGTGAC
GCAGTCGTACGAGTTGATTGTCGCAAAAAAGAAGTGTGGAGCGCCAGTGGCATTAAACGGACCTATGACCGTTTGATCTT
TGCCACAGGCTCCGACCCGTTTATGCTCCCTTTGCCTGGAGCAGAGAAAGAAGGTGTCATTGCGTTTCGCAACATCGCCG
ATTGCGAATCAATGATCAATGCTGCGAAATCGTATCAAAAAGCGGTGGTCATTGGTGCAGGGCTGCTAGGCCTTGAAGCA
GCTAGAGGCCTGTTGAACTTAAAAATGGACGTTGATGTCATCCATATTGCCGATCATTTAATGGACCGGCAACTGGATTC
AGATGCAGGAAAGATGTTGAAGGCTGAGCTTGAACAACAAGGGATGCAGTTTCATTTAAATAAGAAAACGGTCAAAATCA
ATGGCCACAAACGGGTCAAGGGCATTTCCTTCAGTGACCAAACAAGTATAAAAGCTGATTTAGTTGTTATGGCTGTTGGC
ATTCGTCCGAATGTTCAATTGGCTAAGCAATCAGGACTTAAAGTCAATCAAGCAATCGTCGTCAATGATTATATGGAAAC
TAGCGAAGAAGATGTATATGCAGTTGGTGAATGTGCTGAACACCGTGGTATCGTTTATGGGCTTGTTGCTCCCTTGTACG
AACAAGGCAAAGTGCTTGCTGAACACATCTGTACAAATACGAGCAACGAGGTCAGCGGCTATAAAGGGTCAATCGTCTCC
ACTAAATTAAAAGTTTCAGGGGTAAATGTCTTTTCCGCAGGGGCGTTTCGCGATCAAGACGAGACGAAATCGATTCGTAT
CCATGACGAATTTGCTGGAGTCTACAAAAAGCTAGTTGTTAAAAACAACCAAGTTGTAGGGGCCGTCTTATTTGGCGACA
CCAATGATGCCAATCGAATTCTTTCTCTCATTCGGTCTGGGGAAGACATTTCGGCCATAAGTAAAGCGGCACTGTTCCCA
TCTGAGACGAATGACAGCAGTCCATCAGTAGTAGCTACAATGGCAGACGAAGAAACGATTTGCGGCTGTAACGGTGTAAG
CAAAGGCGAGATTGTCTGTGCAATTAAAGAGCAGGGGCTTTCTACGGTAGCAGAAATAAGGCAATGTACGAATGCGTCAA
GGTCTTGTGGTGGCTGCAAGCCGGAAGTAGCTGAATTGTTGGCACTCACAACAGGAACGGACATAATGGCAAAACCAGAA
CCTCTTTGCGGCTGTACGGAATTAACCCATGAAGCAGTGGTAGCCTCGATCAGGGAAATGGGCTTGTCCTATGTTCGAGA
AGTCATTAGCGTGCTTGGTTGGAAGTCAGAGGAAGGCTGTTCAAAGTGCCGCCCCGCTCTCAACTACTATCTAGGTCTCA
TTAGCCCCCTTGATTATACGGATGATGCTCATTCACGGTTTGTTAATGAACGGGTCCACGCCAATATCCAGAAAGACGGC
ACGTTCTCTGTTGTGCCTCGGATGTATGGCGGGGTGACCAATCCAGAACAACTGCGGAAAATCGCTGATGTTGCTGAAAA
ATACAATGTCCCTTTATTAAAAGTCACCGGCGGTCAACGGATCGACTTGCTCGGCGTCGAAAAAGAAAAATTGCCTAACG
TATGGCGTGATTTAGGGATGCGCTCCGGCTATGCGTATGGCAAAACATTGCGGACAGTCAAAACGTGCGTTGGTGAAGAT
TTTTGCCGTTTTGGCACACAGGACTCGATTAGCCTTGGAATCGAAATTGAGAAAAAATATGAGGGCCTGAATACGCCCCA
TAAAGTAAAAATGGCTGTTTCCGCTTGCCCGCGTAATTGTGCGGAATCAGGAATAAAAGATGTTGGAATTGTGGGTGTAG
AAGGAGCATGGGAAATCTATGTAGGCGGGAATGGAGGCGTTGATTTAAGAAGCGCTGAGCTGCTTTGCAAAGTGGAAACG
AATGAAGAAGTAATGGAAACGATCAGTGCATTTTTGCAATATTATCGGGAACAAGCCACCTATTTAGAACGGACTTCCCA
TTGGATTGAGCGAGTCGGGCTTGAATCAATTAAAAAAGTGATTTTCGACGACAGCATCGAACGAGCAGCGCTAATCGAAC
GGATGGAAATGGCGCTATCTAGGCACAAAGATCCGTGGACGGAAATTGTTGAGAGTGAAGAAAAGCAGAATCAATTGTTT
GAAAAGAAAAAAATTGCTGTTAAACAGGGAGGAGGTATGTGA

Upstream 100 bases:

>100_bases
TTTCGATGTACGCTTCTGTCGTTCTGCACAATCAACCACTCGATCAGACAATGTCTTAAACGAGGTAATTGGATTTTTTT
AAAAAAAGGAGTTTTGAGAT

Downstream 100 bases:

>100_bases
TGGCATTCGTGGAAGCGGGTAGAAAAGTGTTTGTCGCTAACCGAAATGAGCTGACGGTCGGTTTAGGCAAGAAAGTTGAA
ATCGAAGGGAGGGAGCTTGC

Product: assimilatory nitrite reductase [NAD(P)H] large subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 813; Mature: 813

Protein sequence:

>813_residues
MNKEKVVLIGNGMAGIRTLEEIIKREPSRFQFTVFGAEPHPNYNRILLSSVLQGDASVSDIVLNDYDWYERNQIELFTGD
AVVRVDCRKKEVWSASGIKRTYDRLIFATGSDPFMLPLPGAEKEGVIAFRNIADCESMINAAKSYQKAVVIGAGLLGLEA
ARGLLNLKMDVDVIHIADHLMDRQLDSDAGKMLKAELEQQGMQFHLNKKTVKINGHKRVKGISFSDQTSIKADLVVMAVG
IRPNVQLAKQSGLKVNQAIVVNDYMETSEEDVYAVGECAEHRGIVYGLVAPLYEQGKVLAEHICTNTSNEVSGYKGSIVS
TKLKVSGVNVFSAGAFRDQDETKSIRIHDEFAGVYKKLVVKNNQVVGAVLFGDTNDANRILSLIRSGEDISAISKAALFP
SETNDSSPSVVATMADEETICGCNGVSKGEIVCAIKEQGLSTVAEIRQCTNASRSCGGCKPEVAELLALTTGTDIMAKPE
PLCGCTELTHEAVVASIREMGLSYVREVISVLGWKSEEGCSKCRPALNYYLGLISPLDYTDDAHSRFVNERVHANIQKDG
TFSVVPRMYGGVTNPEQLRKIADVAEKYNVPLLKVTGGQRIDLLGVEKEKLPNVWRDLGMRSGYAYGKTLRTVKTCVGED
FCRFGTQDSISLGIEIEKKYEGLNTPHKVKMAVSACPRNCAESGIKDVGIVGVEGAWEIYVGGNGGVDLRSAELLCKVET
NEEVMETISAFLQYYREQATYLERTSHWIERVGLESIKKVIFDDSIERAALIERMEMALSRHKDPWTEIVESEEKQNQLF
EKKKIAVKQGGGM

Sequences:

>Translated_813_residues
MNKEKVVLIGNGMAGIRTLEEIIKREPSRFQFTVFGAEPHPNYNRILLSSVLQGDASVSDIVLNDYDWYERNQIELFTGD
AVVRVDCRKKEVWSASGIKRTYDRLIFATGSDPFMLPLPGAEKEGVIAFRNIADCESMINAAKSYQKAVVIGAGLLGLEA
ARGLLNLKMDVDVIHIADHLMDRQLDSDAGKMLKAELEQQGMQFHLNKKTVKINGHKRVKGISFSDQTSIKADLVVMAVG
IRPNVQLAKQSGLKVNQAIVVNDYMETSEEDVYAVGECAEHRGIVYGLVAPLYEQGKVLAEHICTNTSNEVSGYKGSIVS
TKLKVSGVNVFSAGAFRDQDETKSIRIHDEFAGVYKKLVVKNNQVVGAVLFGDTNDANRILSLIRSGEDISAISKAALFP
SETNDSSPSVVATMADEETICGCNGVSKGEIVCAIKEQGLSTVAEIRQCTNASRSCGGCKPEVAELLALTTGTDIMAKPE
PLCGCTELTHEAVVASIREMGLSYVREVISVLGWKSEEGCSKCRPALNYYLGLISPLDYTDDAHSRFVNERVHANIQKDG
TFSVVPRMYGGVTNPEQLRKIADVAEKYNVPLLKVTGGQRIDLLGVEKEKLPNVWRDLGMRSGYAYGKTLRTVKTCVGED
FCRFGTQDSISLGIEIEKKYEGLNTPHKVKMAVSACPRNCAESGIKDVGIVGVEGAWEIYVGGNGGVDLRSAELLCKVET
NEEVMETISAFLQYYREQATYLERTSHWIERVGLESIKKVIFDDSIERAALIERMEMALSRHKDPWTEIVESEEKQNQLF
EKKKIAVKQGGGM
>Mature_813_residues
MNKEKVVLIGNGMAGIRTLEEIIKREPSRFQFTVFGAEPHPNYNRILLSSVLQGDASVSDIVLNDYDWYERNQIELFTGD
AVVRVDCRKKEVWSASGIKRTYDRLIFATGSDPFMLPLPGAEKEGVIAFRNIADCESMINAAKSYQKAVVIGAGLLGLEA
ARGLLNLKMDVDVIHIADHLMDRQLDSDAGKMLKAELEQQGMQFHLNKKTVKINGHKRVKGISFSDQTSIKADLVVMAVG
IRPNVQLAKQSGLKVNQAIVVNDYMETSEEDVYAVGECAEHRGIVYGLVAPLYEQGKVLAEHICTNTSNEVSGYKGSIVS
TKLKVSGVNVFSAGAFRDQDETKSIRIHDEFAGVYKKLVVKNNQVVGAVLFGDTNDANRILSLIRSGEDISAISKAALFP
SETNDSSPSVVATMADEETICGCNGVSKGEIVCAIKEQGLSTVAEIRQCTNASRSCGGCKPEVAELLALTTGTDIMAKPE
PLCGCTELTHEAVVASIREMGLSYVREVISVLGWKSEEGCSKCRPALNYYLGLISPLDYTDDAHSRFVNERVHANIQKDG
TFSVVPRMYGGVTNPEQLRKIADVAEKYNVPLLKVTGGQRIDLLGVEKEKLPNVWRDLGMRSGYAYGKTLRTVKTCVGED
FCRFGTQDSISLGIEIEKKYEGLNTPHKVKMAVSACPRNCAESGIKDVGIVGVEGAWEIYVGGNGGVDLRSAELLCKVET
NEEVMETISAFLQYYREQATYLERTSHWIERVGLESIKKVIFDDSIERAALIERMEMALSRHKDPWTEIVESEEKQNQLF
EKKKIAVKQGGGM

Specific function: Required for nitrite assimilation [H]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI226437568, Length=275, Percent_Identity=28, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI65787454, Length=275, Percent_Identity=28, Blast_Score=108, Evalue=2e-23,
Organism=Homo sapiens, GI21389617, Length=275, Percent_Identity=28, Blast_Score=108, Evalue=2e-23,
Organism=Homo sapiens, GI4757732, Length=234, Percent_Identity=26.9230769230769, Blast_Score=67, Evalue=6e-11,
Organism=Homo sapiens, GI22202629, Length=234, Percent_Identity=26.9230769230769, Blast_Score=67, Evalue=6e-11,
Organism=Escherichia coli, GI1789765, Length=817, Percent_Identity=35.3733170134639, Blast_Score=466, Evalue=1e-132,
Organism=Escherichia coli, GI1789065, Length=339, Percent_Identity=27.4336283185841, Blast_Score=124, Evalue=3e-29,
Organism=Escherichia coli, GI1788892, Length=281, Percent_Identity=25.9786476868327, Blast_Score=96, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17559934, Length=417, Percent_Identity=23.9808153477218, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI281359715, Length=309, Percent_Identity=24.5954692556634, Blast_Score=109, Evalue=1e-23,
Organism=Drosophila melanogaster, GI281359713, Length=309, Percent_Identity=24.5954692556634, Blast_Score=109, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24639250, Length=309, Percent_Identity=24.5954692556634, Blast_Score=109, Evalue=1e-23,
Organism=Drosophila melanogaster, GI18543267, Length=309, Percent_Identity=24.5954692556634, Blast_Score=109, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24639252, Length=309, Percent_Identity=24.5954692556634, Blast_Score=109, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24639257, Length=309, Percent_Identity=24.5954692556634, Blast_Score=108, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24585130, Length=283, Percent_Identity=22.2614840989399, Blast_Score=92, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 89436; Mature: 89436

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKEKVVLIGNGMAGIRTLEEIIKREPSRFQFTVFGAEPHPNYNRILLSSVLQGDASVSD
CCCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCCE
IVLNDYDWYERNQIELFTGDAVVRVDCRKKEVWSASGIKRTYDRLIFATGSDPFMLPLPG
EEECCCCCCCCCEEEEEECCEEEEEECCCCHHHCCCCHHHHHCEEEEEECCCCEEEECCC
AEKEGVIAFRNIADCESMINAAKSYQKAVVIGAGLLGLEAARGLLNLKMDVDVIHIADHL
CCCCCEEEECCHHHHHHHHHHHHHHHCEEEEECCHHHHHHHCCCEEEEECCCHHHHHHHH
MDRQLDSDAGKMLKAELEQQGMQFHLNKKTVKINGHKRVKGISFSDQTSIKADLVVMAVG
HHHHCCCHHHHHHHHHHHHCCCEEEECCEEEEECCCCEEECCCCCCCCCCEEEEEEEEEC
IRPNVQLAKQSGLKVNQAIVVNDYMETSEEDVYAVGECAEHRGIVYGLVAPLYEQGKVLA
CCCCCHHHHHCCCEECEEEEEECHHCCCCCCEEEHHHHHHHCCEEEEHHHHHHHCCHHHH
EHICTNTSNEVSGYKGSIVSTKLKVSGVNVFSAGAFRDQDETKSIRIHDEFAGVYKKLVV
HHHHCCCCCCCCCCCCCEEEEEEEEECCEEEECCCCCCCCCCCEEEEEHHHHHHHHHHHH
KNNQVVGAVLFGDTNDANRILSLIRSGEDISAISKAALFPSETNDSSPSVVATMADEETI
CCCCEEEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCEEEEECCCCCE
CGCNGVSKGEIVCAIKEQGLSTVAEIRQCTNASRSCGGCKPEVAELLALTTGTDIMAKPE
ECCCCCCCCCEEEEEHHCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCC
PLCGCTELTHEAVVASIREMGLSYVREVISVLGWKSEEGCSKCRPALNYYLGLISPLDYT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCC
DDAHSRFVNERVHANIQKDGTFSVVPRMYGGVTNPEQLRKIADVAEKYNVPLLKVTGGQR
CCHHHHHHHHHHHCCCCCCCCEEECHHHHCCCCCHHHHHHHHHHHHHHCCCEEEECCCCE
IDLLGVEKEKLPNVWRDLGMRSGYAYGKTLRTVKTCVGEDFCRFGTQDSISLGIEIEKKY
EEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCHHHHHCCCCCCEEEEEEEEHHH
EGLNTPHKVKMAVSACPRNCAESGIKDVGIVGVEGAWEIYVGGNGGVDLRSAELLCKVET
CCCCCCHHEEHHHHHCCHHHHHCCCCCCEEEEECCEEEEEECCCCCCCEECCEEEEEECC
NEEVMETISAFLQYYREQATYLERTSHWIERVGLESIKKVIFDDSIERAALIERMEMALS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHHHH
RHKDPWTEIVESEEKQNQLFEKKKIAVKQGGGM
HCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNKEKVVLIGNGMAGIRTLEEIIKREPSRFQFTVFGAEPHPNYNRILLSSVLQGDASVSD
CCCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCCE
IVLNDYDWYERNQIELFTGDAVVRVDCRKKEVWSASGIKRTYDRLIFATGSDPFMLPLPG
EEECCCCCCCCCEEEEEECCEEEEEECCCCHHHCCCCHHHHHCEEEEEECCCCEEEECCC
AEKEGVIAFRNIADCESMINAAKSYQKAVVIGAGLLGLEAARGLLNLKMDVDVIHIADHL
CCCCCEEEECCHHHHHHHHHHHHHHHCEEEEECCHHHHHHHCCCEEEEECCCHHHHHHHH
MDRQLDSDAGKMLKAELEQQGMQFHLNKKTVKINGHKRVKGISFSDQTSIKADLVVMAVG
HHHHCCCHHHHHHHHHHHHCCCEEEECCEEEEECCCCEEECCCCCCCCCCEEEEEEEEEC
IRPNVQLAKQSGLKVNQAIVVNDYMETSEEDVYAVGECAEHRGIVYGLVAPLYEQGKVLA
CCCCCHHHHHCCCEECEEEEEECHHCCCCCCEEEHHHHHHHCCEEEEHHHHHHHCCHHHH
EHICTNTSNEVSGYKGSIVSTKLKVSGVNVFSAGAFRDQDETKSIRIHDEFAGVYKKLVV
HHHHCCCCCCCCCCCCCEEEEEEEEECCEEEECCCCCCCCCCCEEEEEHHHHHHHHHHHH
KNNQVVGAVLFGDTNDANRILSLIRSGEDISAISKAALFPSETNDSSPSVVATMADEETI
CCCCEEEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCEEEEECCCCCE
CGCNGVSKGEIVCAIKEQGLSTVAEIRQCTNASRSCGGCKPEVAELLALTTGTDIMAKPE
ECCCCCCCCCEEEEEHHCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCC
PLCGCTELTHEAVVASIREMGLSYVREVISVLGWKSEEGCSKCRPALNYYLGLISPLDYT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCC
DDAHSRFVNERVHANIQKDGTFSVVPRMYGGVTNPEQLRKIADVAEKYNVPLLKVTGGQR
CCHHHHHHHHHHHCCCCCCCCEEECHHHHCCCCCHHHHHHHHHHHHHHCCCEEEECCCCE
IDLLGVEKEKLPNVWRDLGMRSGYAYGKTLRTVKTCVGEDFCRFGTQDSISLGIEIEKKY
EEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCHHHHHCCCCCCEEEEEEEEHHH
EGLNTPHKVKMAVSACPRNCAESGIKDVGIVGVEGAWEIYVGGNGGVDLRSAELLCKVET
CCCCCCHHEEHHHHHCCHHHHHCCCCCCEEEEECCEEEEEECCCCCCCEECCEEEEEECC
NEEVMETISAFLQYYREQATYLERTSHWIERVGLESIKKVIFDDSIERAALIERMEMALS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHHHH
RHKDPWTEIVESEEKQNQLFEKKKIAVKQGGGM
HCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]