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The map label for this gene is nasF [H]

Identifier: 56963385

GI number: 56963385

Start: 1746965

End: 1748338

Strand: Direct

Name: nasF [H]

Synonym: ABC1620

Alternate gene names: 56963385

Gene position: 1746965-1748338 (Clockwise)

Preceding gene: 56963384

Following gene: 56963386

Centisome position: 40.59

GC content: 47.23

Gene sequence:

>1374_bases
ATGTCCATACAAGGAAAGGTGTACCTCGTCGGTGCAGGGCCTGGAGACGCCGAACTGTTGACTGTTAAAGCACGAAAGGT
ATTACAACAAGCTGACGTTGTGATTTTTGACAGGCTTGCCAATCCTGCGCTCATCATGGAAGTTAGCGATCATGCCAAGT
TGGTGTATGCAGGCAAGCAGCCGTGTAAACATGTGTTGCGCCAAGGAGACATCCAAACAGAAATGCTTGTCCACGCAAAA
AAAGGAAAAACGGTTGTTCGCTTAAAAGGGGGCGATCCTGCCGTATTCGGCAGAGTAGGAGAGGAAGCTGCCTATTTAAA
AACTCACCACATTCCATTTGAGATTGTCCCTGGTGTGACAGCGGGTACAGCCGCTTCTATTTATGCAGGTGTACCGGCAA
CCCATAGAACGCTTTCCAGTTCATTTGCAGTCGTAACTGCTTATCGCGACAGAGACGAAAAAAAAGAGCCCCCTAATTGG
CGAGCACTTGCTCAATCGGTTGACACGCTAATGATCTATATGGGCATGAAACAATTAGCTGCCATTGTTGACCAGTTAAT
GACACATGGAAAACCAGCCGGTACGCCTGTGCTCATTGTCGAGTGGGGCACATACAGTCGACAGCGGTCCGTGGAAGGAA
CACTAGAAACGATTGTGACCAATGTCGCTAACGCTAACTTGGCGAATCCTGCTGTTATTTTAATCGGCGATGTTGTCGGT
GTCCGTGGGGCTGTATCATGGTTTGAGCACAAGCCCCTTTCTGGCATGGGAATACTTTCGCTAAGAGGAGAAACTGAAAT
GACGGGAACGTTGCGGGCGCAAGGGGCGGATGTCTTTGCGGCCCCCCTCCAACAAAACAAGGGGAAAATTGTTACTGATA
CGGACATCGCTGCTGTGCTTCAAACTAGTAAAAATCAAGCAGTCTTGTTTTTTGCCAAGGAGGTGTTGTTCGCGTTTTTA
GCAAAGCTTGGCGAGAAAGGCTATGATATTCGTTCTGTACAAGGGCAACTTATGGCGGGAACACAGGAGGTGGAGCAAAT
CGCTCGCTCACTCGGTTTGCAATTGGCGCGTTATTCGAAAAAATCAACTCTTTCGCCTGTCGTGATTGGTACAGATGCCA
TTAACCGCCGCTTGTTACCACAAAAAATAACTGTTGCGATTCGTCGTCTGCTAGAAGAAGGCCACTTAACTCATGCCTTT
TGTGAAACAGAGCAAGAAATTGATGATTTGCGACTCGTTTTAGCAGAATGTGCAAATGAAGCTGTATTGCCTATTTTAAC
GACGAGCGATCAAGTTCAAGCATATGCTAAGTCTCTTTCGATGTACGCTTCTGTCGTTCTGCACAATCAACCACTCGATC
AGACAATGTCTTAA

Upstream 100 bases:

>100_bases
GAACGTTAGTATGTCACAAAATATTACAATCGGTGCCAGTAGAAAAACGATCCACGTTAGACTAGGGGAAATCATTTTAG
GTATCAAAAAGGAGTTTTAC

Downstream 100 bases:

>100_bases
ACGAGGTAATTGGATTTTTTTAAAAAAAGGAGTTTTGAGATGTGAATAAAGAAAAGGTTGTTTTAATTGGCAATGGCATG
GCGGGCATCCGCACACTCGA

Product: uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM [H]

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MSIQGKVYLVGAGPGDAELLTVKARKVLQQADVVIFDRLANPALIMEVSDHAKLVYAGKQPCKHVLRQGDIQTEMLVHAK
KGKTVVRLKGGDPAVFGRVGEEAAYLKTHHIPFEIVPGVTAGTAASIYAGVPATHRTLSSSFAVVTAYRDRDEKKEPPNW
RALAQSVDTLMIYMGMKQLAAIVDQLMTHGKPAGTPVLIVEWGTYSRQRSVEGTLETIVTNVANANLANPAVILIGDVVG
VRGAVSWFEHKPLSGMGILSLRGETEMTGTLRAQGADVFAAPLQQNKGKIVTDTDIAAVLQTSKNQAVLFFAKEVLFAFL
AKLGEKGYDIRSVQGQLMAGTQEVEQIARSLGLQLARYSKKSTLSPVVIGTDAINRRLLPQKITVAIRRLLEEGHLTHAF
CETEQEIDDLRLVLAECANEAVLPILTTSDQVQAYAKSLSMYASVVLHNQPLDQTMS

Sequences:

>Translated_457_residues
MSIQGKVYLVGAGPGDAELLTVKARKVLQQADVVIFDRLANPALIMEVSDHAKLVYAGKQPCKHVLRQGDIQTEMLVHAK
KGKTVVRLKGGDPAVFGRVGEEAAYLKTHHIPFEIVPGVTAGTAASIYAGVPATHRTLSSSFAVVTAYRDRDEKKEPPNW
RALAQSVDTLMIYMGMKQLAAIVDQLMTHGKPAGTPVLIVEWGTYSRQRSVEGTLETIVTNVANANLANPAVILIGDVVG
VRGAVSWFEHKPLSGMGILSLRGETEMTGTLRAQGADVFAAPLQQNKGKIVTDTDIAAVLQTSKNQAVLFFAKEVLFAFL
AKLGEKGYDIRSVQGQLMAGTQEVEQIARSLGLQLARYSKKSTLSPVVIGTDAINRRLLPQKITVAIRRLLEEGHLTHAF
CETEQEIDDLRLVLAECANEAVLPILTTSDQVQAYAKSLSMYASVVLHNQPLDQTMS
>Mature_456_residues
SIQGKVYLVGAGPGDAELLTVKARKVLQQADVVIFDRLANPALIMEVSDHAKLVYAGKQPCKHVLRQGDIQTEMLVHAKK
GKTVVRLKGGDPAVFGRVGEEAAYLKTHHIPFEIVPGVTAGTAASIYAGVPATHRTLSSSFAVVTAYRDRDEKKEPPNWR
ALAQSVDTLMIYMGMKQLAAIVDQLMTHGKPAGTPVLIVEWGTYSRQRSVEGTLETIVTNVANANLANPAVILIGDVVGV
RGAVSWFEHKPLSGMGILSLRGETEMTGTLRAQGADVFAAPLQQNKGKIVTDTDIAAVLQTSKNQAVLFFAKEVLFAFLA
KLGEKGYDIRSVQGQLMAGTQEVEQIARSLGLQLARYSKKSTLSPVVIGTDAINRRLLPQKITVAIRRLLEEGHLTHAFC
ETEQEIDDLRLVLAECANEAVLPILTTSDQVQAYAKSLSMYASVVLHNQPLDQTMS

Specific function: Multifunctional Enzyme That Catalyze The Sam-Dependent Methylation Of Uroporphyrinogen III At Position C-2 And C-7 To Form Precorrin-2 And Then Position C-12 Or C-18 To Form Trimethylpyrrocorphin 2. It Also Catalyzes The Conversion Of Precorrin-2 Into Sir

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=247, Percent_Identity=43.7246963562753, Blast_Score=201, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6322922, Length=237, Percent_Identity=29.535864978903, Blast_Score=106, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR003754
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF02602 HEM4; PF00590 TP_methylase [H]

EC number: =2.1.1.107 [H]

Molecular weight: Translated: 49483; Mature: 49352

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIQGKVYLVGAGPGDAELLTVKARKVLQQADVVIFDRLANPALIMEVSDHAKLVYAGKQ
CCCCCEEEEEECCCCCCEEEEHHHHHHHHHCCCCEECCCCCCEEEEEECCCCEEEEECCH
PCKHVLRQGDIQTEMLVHAKKGKTVVRLKGGDPAVFGRVGEEAAYLKTHHIPFEIVPGVT
HHHHHHHCCCCCHHHEEEECCCCEEEEEECCCCEEECCCCCCHHHHEECCCCEEEECCCC
AGTAASIYAGVPATHRTLSSSFAVVTAYRDRDEKKEPPNWRALAQSVDTLMIYMGMKQLA
CCCHHHHHCCCCHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AIVDQLMTHGKPAGTPVLIVEWGTYSRQRSVEGTLETIVTNVANANLANPAVILIGDVVG
HHHHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECHHH
VRGAVSWFEHKPLSGMGILSLRGETEMTGTLRAQGADVFAAPLQQNKGKIVTDTDIAAVL
HHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEECCCCEEEECHHCCCCCEEECCHHHHHH
QTSKNQAVLFFAKEVLFAFLAKLGEKGYDIRSVQGQLMAGTQEVEQIARSLGLQLARYSK
HCCCCCEEEEHHHHHHHHHHHHHCCCCCEEHHCCCHHHCCHHHHHHHHHHHCHHHHHHCC
KSTLSPVVIGTDAINRRLLPQKITVAIRRLLEEGHLTHAFCETEQEIDDLRLVLAECANE
CCCCCCEEEECHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHCHHHHHHHHHHHHHHCCC
AVLPILTTSDQVQAYAKSLSMYASVVLHNQPLDQTMS
CEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCHHCCC
>Mature Secondary Structure 
SIQGKVYLVGAGPGDAELLTVKARKVLQQADVVIFDRLANPALIMEVSDHAKLVYAGKQ
CCCCEEEEEECCCCCCEEEEHHHHHHHHHCCCCEECCCCCCEEEEEECCCCEEEEECCH
PCKHVLRQGDIQTEMLVHAKKGKTVVRLKGGDPAVFGRVGEEAAYLKTHHIPFEIVPGVT
HHHHHHHCCCCCHHHEEEECCCCEEEEEECCCCEEECCCCCCHHHHEECCCCEEEECCCC
AGTAASIYAGVPATHRTLSSSFAVVTAYRDRDEKKEPPNWRALAQSVDTLMIYMGMKQLA
CCCHHHHHCCCCHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AIVDQLMTHGKPAGTPVLIVEWGTYSRQRSVEGTLETIVTNVANANLANPAVILIGDVVG
HHHHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECHHH
VRGAVSWFEHKPLSGMGILSLRGETEMTGTLRAQGADVFAAPLQQNKGKIVTDTDIAAVL
HHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEECCCCEEEECHHCCCCCEEECCHHHHHH
QTSKNQAVLFFAKEVLFAFLAKLGEKGYDIRSVQGQLMAGTQEVEQIARSLGLQLARYSK
HCCCCCEEEEHHHHHHHHHHHHHCCCCCEEHHCCCHHHCCHHHHHHHHHHHCHHHHHHCC
KSTLSPVVIGTDAINRRLLPQKITVAIRRLLEEGHLTHAFCETEQEIDDLRLVLAECANE
CCCCCCEEEECHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHCHHHHHHHHHHHHHHCCC
AVLPILTTSDQVQAYAKSLSMYASVVLHNQPLDQTMS
CEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]