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The map label for this gene is sacP [H]

Identifier: 56963218

GI number: 56963218

Start: 1570569

End: 1571975

Strand: Direct

Name: sacP [H]

Synonym: ABC1453

Alternate gene names: 56963218

Gene position: 1570569-1571975 (Clockwise)

Preceding gene: 56963217

Following gene: 56963219

Centisome position: 36.49

GC content: 46.34

Gene sequence:

>1407_bases
ATGGGGCCTGAAAAGGAATATAAAAAAATTGCCCAGGATGTAATTAAAGGGCTTGGTGGAAAAGAAAATATCATATCGAT
GGCACACTGTGCCACACGACTTAGACTGATTGTCAAGGATCGGGATATCATTGATGATGAATTTATTGAGAACGTGGACA
AGGCTAAAGGGGTCTTTTTTACTTCTGGGCAATATCAAATCATTTTTGGAACCGGTACCGTCAATCGAGTATATGAGGCC
ATGATCGGAGAGGACGTCTCTTCTACTTCCAAAGCGGAATTGAAAGAGAAGGCTTCTGAGCAAGATGATCATTTCTTTAA
ACGAATGATCCGGGTGTTCGGGGACGTATTCGTACCGATTATCCCAGCGTTGGTTGCTACGGGCTTATTCATGGGTGTCA
GAGGCCTTATTACGCAGCCTGCCATCCTCGCGTGGTTTGGACTTACTCCAGACAGCATTTCCGACAATTTCTTAGTGTTC
ACGCAAATTTTGACAGACACTGCTTTTGGTTTCTTGCCCGTGCTCGTATGTTGGTCAACTTTTCGGGTGTTTGGCGGTTC
ACCGCTGCTTGGGATTGTGCTCGGCTTGATGCTCGTCAGCCCTTCCCTGCCAACGTCCTGGGATGTCGCCCAGAATGTTC
AGGAACCGCTAATGTTCCTTGGATTTATTAAAGTAGCCGGTTATCAGGGCTCGGTGTTGCCTGCGTTTATTATCGGTATA
GTTGGTGCGTTGCTAGAGAAAAGAATCCGCAAACTCGTTCCCGCTGCGTTGGATTTAATTTTGACGCCATTTTTAACATT
GCTGATCGCTCTAATGCTAGGACTGTTTGTGCTCGGTCCTGTGTTCCATGAAGTAGAGATTTTAATCTTGACAGCAGTTA
CATGGATTCTGCAGTTGCCGTTCGGAATTGGCGGCTTCATCTATGGTGGCATTAATCAATTTATCGTTGTGACGGGGCTT
CATCATGCCTTAAACTTGATCGAAATTCAAATGTTAAGTGACACTGGCTGGAATATGGTCAATGCAATCAGCTCTGGTTC
GATCGCTGCACAAGCGGGAGCCGCGTTGGCTGTTGGCTTGAAGGCGAAAAGCCTCAAGACGAAGTCGATTGCTTACCCTT
CTTCCTTGTCTGCTTTGCTCGGCATTACGGAGCCAGCTATTTTCGGTGTGAATATCCGTTATGGCAAGCCGTTCCTGATG
GGCTTGATCGGTGGCGCCGTTGCTGGATTCTTTGCCCGGATCCTCGATGTTCAGGCAACAGGCATGTCGATTACAGTCAT
TCCAGGTATGTTGCTGTACTTAAACGCGCAAATCATTCCGTACATTGCAGTCTGCGTGATTGGATTTGGGATAGCGTTTG
CCCTTACATGGATGTTTGGCTTTAAAGAGAAAACAAATCTGAAATAA

Upstream 100 bases:

>100_bases
ATTTGCCCTTAATCACGTAAAATAATAGCGTTGATTGTAAGCGCCATCTTGATATGCTTAGAAGCAGGAAGCTACTTAAT
TAGTTGAAAGGAAGAGACAG

Downstream 100 bases:

>100_bases
ACAAATTAGGGGGAGAACCATGCAAAATAATCAGCTGCTTGAGAAATTGGGCAGTCTTACTGTTGAAGAGAAAATCGGCC
AGCTTGTACAGCTGACTGGT

Product: PTS system, sucrose-specific enzyme II, BC component

Products: NA

Alternate protein names: EIIBC-Scr; EII-Scr; Sucrose-specific phosphotransferase enzyme IIB component; PTS system sucrose-specific EIIB component; Sucrose permease IIC component; PTS system sucrose-specific EIIC component [H]

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MGPEKEYKKIAQDVIKGLGGKENIISMAHCATRLRLIVKDRDIIDDEFIENVDKAKGVFFTSGQYQIIFGTGTVNRVYEA
MIGEDVSSTSKAELKEKASEQDDHFFKRMIRVFGDVFVPIIPALVATGLFMGVRGLITQPAILAWFGLTPDSISDNFLVF
TQILTDTAFGFLPVLVCWSTFRVFGGSPLLGIVLGLMLVSPSLPTSWDVAQNVQEPLMFLGFIKVAGYQGSVLPAFIIGI
VGALLEKRIRKLVPAALDLILTPFLTLLIALMLGLFVLGPVFHEVEILILTAVTWILQLPFGIGGFIYGGINQFIVVTGL
HHALNLIEIQMLSDTGWNMVNAISSGSIAAQAGAALAVGLKAKSLKTKSIAYPSSLSALLGITEPAIFGVNIRYGKPFLM
GLIGGAVAGFFARILDVQATGMSITVIPGMLLYLNAQIIPYIAVCVIGFGIAFALTWMFGFKEKTNLK

Sequences:

>Translated_468_residues
MGPEKEYKKIAQDVIKGLGGKENIISMAHCATRLRLIVKDRDIIDDEFIENVDKAKGVFFTSGQYQIIFGTGTVNRVYEA
MIGEDVSSTSKAELKEKASEQDDHFFKRMIRVFGDVFVPIIPALVATGLFMGVRGLITQPAILAWFGLTPDSISDNFLVF
TQILTDTAFGFLPVLVCWSTFRVFGGSPLLGIVLGLMLVSPSLPTSWDVAQNVQEPLMFLGFIKVAGYQGSVLPAFIIGI
VGALLEKRIRKLVPAALDLILTPFLTLLIALMLGLFVLGPVFHEVEILILTAVTWILQLPFGIGGFIYGGINQFIVVTGL
HHALNLIEIQMLSDTGWNMVNAISSGSIAAQAGAALAVGLKAKSLKTKSIAYPSSLSALLGITEPAIFGVNIRYGKPFLM
GLIGGAVAGFFARILDVQATGMSITVIPGMLLYLNAQIIPYIAVCVIGFGIAFALTWMFGFKEKTNLK
>Mature_467_residues
GPEKEYKKIAQDVIKGLGGKENIISMAHCATRLRLIVKDRDIIDDEFIENVDKAKGVFFTSGQYQIIFGTGTVNRVYEAM
IGEDVSSTSKAELKEKASEQDDHFFKRMIRVFGDVFVPIIPALVATGLFMGVRGLITQPAILAWFGLTPDSISDNFLVFT
QILTDTAFGFLPVLVCWSTFRVFGGSPLLGIVLGLMLVSPSLPTSWDVAQNVQEPLMFLGFIKVAGYQGSVLPAFIIGIV
GALLEKRIRKLVPAALDLILTPFLTLLIALMLGLFVLGPVFHEVEILILTAVTWILQLPFGIGGFIYGGINQFIVVTGLH
HALNLIEIQMLSDTGWNMVNAISSGSIAAQAGAALAVGLKAKSLKTKSIAYPSSLSALLGITEPAIFGVNIRYGKPFLMG
LIGGAVAGFFARILDVQATGMSITVIPGMLLYLNAQIIPYIAVCVIGFGIAFALTWMFGFKEKTNLK

Specific function: Acts as both a kinase and a phosphatase on sacT [H]

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI2367362, Length=425, Percent_Identity=31.5294117647059, Blast_Score=222, Evalue=4e-59,
Organism=Escherichia coli, GI1790159, Length=467, Percent_Identity=28.6937901498929, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI1788769, Length=430, Percent_Identity=27.906976744186, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI48994906, Length=475, Percent_Identity=25.4736842105263, Blast_Score=130, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018113
- InterPro:   IPR004719
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR010973 [H]

Pfam domain/function: PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 50575; Mature: 50444

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CEEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
GPEKEYKKIAQDVIKGLGGKENIISMAHCATRLRLIVKDRDIIDDEFIENVDKAKGVFF
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCEEE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CEEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3122206; 7934828; 9384377; 10568751 [H]