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The map label for this gene is sbp [H]

Identifier: 56962998

GI number: 56962998

Start: 1338351

End: 1339373

Strand: Direct

Name: sbp [H]

Synonym: ABC1226

Alternate gene names: 56962998

Gene position: 1338351-1339373 (Clockwise)

Preceding gene: 56962993

Following gene: 56962999

Centisome position: 31.1

GC content: 47.02

Gene sequence:

>1023_bases
ATGAAAAAGGGATATGGTGTGTCTGTCGCGACTGTAGGAGTGCTGCTTGCGGCTTGTGCTCCTTCAGGTGAAGAAACATC
AGGGGAGGGGAATTCAGTTGAGATTTTAAACGTATCTTACGACCCAACTAGAGAATTATATGCAGACGTAAACAAGGCAT
TTGCTGACCATTGGTTGGAAAAAACCGGTGAGCACGTAGCTATTAACACTTCCCACGGTGGTTCAGGCAGCCAAGCGCGC
TCCGTTCTCGACGGCTTGGAGGCAGATGTCGTTACATTAGCCCTTGCTTATGATATTGATATTTTGCAAGAACGGGAGCT
GCTTGATCCAAATTGGCAGCAGCAATTTGCCGATCAATCTTCTCCTTATACGTCGACGATTAATTTTCTTGTCCGCAAAG
GGAACCCAAAACAAATTGAAGATTGGGATGACCTCATTCGCGATGATGTGGAAGTGGTGACGCCAAATCCGAAAACGTCA
GGCGGGGCACGATGGAACTATTTAGCGGCTTGGGGTTACGCAGAAGAAACTTATGGTTCAGAGGAAGCAGCGCAACAATT
TGTTGGAGACTTGTACCAAAATGTAACTGCATTAGATTCAGGCGCTAGGGGCGCTACGACTTCTTTTGTGGAACGGGGCA
TTGGCGATGTTTTGTTGGCATGGGAAAATGAAGCGATTCTTGCCGCAAATGAGCTTGGCGATTCTGAGTTTGATATTGTG
ACACCGTCGATGTCGATTTTAACGGAACCTCCTGTTGCCATTGTCGATAAAGTCGTCGACCAAAAAGGGACGAGAGAAGT
AGCGGAAGCCTATTTGCAATTCCTTTACAGCGATGAAGGGCAGGAGTTGATCGCCGACCATTATTACCGGCCACGGAATG
AAGCAGTTTTAGAGGAACATCGCGATGTGTTCCCGGAAGTAGAGCTATTTACAATTGACGATAAATTTGGCGGCTGGCAA
GAGGCGCAGGACAAACATTTTGCTGACGGCGGTATATTTGACCAGATATACCAGCCCAATTAA

Upstream 100 bases:

>100_bases
TCTTTTAACGAATCCGTCTATAGCGGTTTCTTCTTTTCGCTCAAAAACCAAGTAATTATCTATTTTTAATGCATTAAATC
GGATTAAAGGGGTGTATTCT

Downstream 100 bases:

>100_bases
GGTGAGCGTTGCCGCCTTGATGTTCCATAAGCAGCATGGAACAACTAGAAAAACAGACGATAGGAGTTTGCTATGAAACG
GATATTGCCGGGATTTGGCT

Product: sulfate ABC transporter substrate-binding protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: Sulfate starvation-induced protein 2; SSI2 [H]

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MKKGYGVSVATVGVLLAACAPSGEETSGEGNSVEILNVSYDPTRELYADVNKAFADHWLEKTGEHVAINTSHGGSGSQAR
SVLDGLEADVVTLALAYDIDILQERELLDPNWQQQFADQSSPYTSTINFLVRKGNPKQIEDWDDLIRDDVEVVTPNPKTS
GGARWNYLAAWGYAEETYGSEEAAQQFVGDLYQNVTALDSGARGATTSFVERGIGDVLLAWENEAILAANELGDSEFDIV
TPSMSILTEPPVAIVDKVVDQKGTREVAEAYLQFLYSDEGQELIADHYYRPRNEAVLEEHRDVFPEVELFTIDDKFGGWQ
EAQDKHFADGGIFDQIYQPN

Sequences:

>Translated_340_residues
MKKGYGVSVATVGVLLAACAPSGEETSGEGNSVEILNVSYDPTRELYADVNKAFADHWLEKTGEHVAINTSHGGSGSQAR
SVLDGLEADVVTLALAYDIDILQERELLDPNWQQQFADQSSPYTSTINFLVRKGNPKQIEDWDDLIRDDVEVVTPNPKTS
GGARWNYLAAWGYAEETYGSEEAAQQFVGDLYQNVTALDSGARGATTSFVERGIGDVLLAWENEAILAANELGDSEFDIV
TPSMSILTEPPVAIVDKVVDQKGTREVAEAYLQFLYSDEGQELIADHYYRPRNEAVLEEHRDVFPEVELFTIDDKFGGWQ
EAQDKHFADGGIFDQIYQPN
>Mature_340_residues
MKKGYGVSVATVGVLLAACAPSGEETSGEGNSVEILNVSYDPTRELYADVNKAFADHWLEKTGEHVAINTSHGGSGSQAR
SVLDGLEADVVTLALAYDIDILQERELLDPNWQQQFADQSSPYTSTINFLVRKGNPKQIEDWDDLIRDDVEVVTPNPKTS
GGARWNYLAAWGYAEETYGSEEAAQQFVGDLYQNVTALDSGARGATTSFVERGIGDVLLAWENEAILAANELGDSEFDIV
TPSMSILTEPPVAIVDKVVDQKGTREVAEAYLQFLYSDEGQELIADHYYRPRNEAVLEEHRDVFPEVELFTIDDKFGGWQ
EAQDKHFADGGIFDQIYQPN

Specific function: This protein specifically binds sulfate and is involved in its transmembrane transport [H]

COG id: COG1613

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1790351, Length=312, Percent_Identity=64.4230769230769, Blast_Score=427, Evalue=1e-121,
Organism=Escherichia coli, GI1788765, Length=312, Percent_Identity=45.8333333333333, Blast_Score=294, Evalue=7e-81,

Paralogues:

None

Copy number: 1240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 37498; Mature: 37498

Theoretical pI: Translated: 3.97; Mature: 3.97

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00757 PROK_SULFATE_BIND_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKGYGVSVATVGVLLAACAPSGEETSGEGNSVEILNVSYDPTRELYADVNKAFADHWLE
CCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHH
KTGEHVAINTSHGGSGSQARSVLDGLEADVVTLALAYDIDILQERELLDPNWQQQFADQS
HCCCEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCC
SPYTSTINFLVRKGNPKQIEDWDDLIRDDVEVVTPNPKTSGGARWNYLAAWGYAEETYGS
CCHHHEEEEEEECCCCCCCHHHHHHHHCCCEEECCCCCCCCCCCEEEEEEECCHHHHCCC
EEAAQQFVGDLYQNVTALDSGARGATTSFVERGIGDVLLAWENEAILAANELGDSEFDIV
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCEEEEECCCEEEEEHHCCCCCCEEE
TPSMSILTEPPVAIVDKVVDQKGTREVAEAYLQFLYSDEGQELIADHYYRPRNEAVLEEH
CCCHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHH
RDVFPEVELFTIDDKFGGWQEAQDKHFADGGIFDQIYQPN
HHCCCCEEEEEEECCCCCCHHHCCCCCCCCCCHHHCCCCC
>Mature Secondary Structure
MKKGYGVSVATVGVLLAACAPSGEETSGEGNSVEILNVSYDPTRELYADVNKAFADHWLE
CCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHH
KTGEHVAINTSHGGSGSQARSVLDGLEADVVTLALAYDIDILQERELLDPNWQQQFADQS
HCCCEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCC
SPYTSTINFLVRKGNPKQIEDWDDLIRDDVEVVTPNPKTSGGARWNYLAAWGYAEETYGS
CCHHHEEEEEEECCCCCCCHHHHHHHHCCCEEECCCCCCCCCCCEEEEEEECCHHHHCCC
EEAAQQFVGDLYQNVTALDSGARGATTSFVERGIGDVLLAWENEAILAANELGDSEFDIV
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCEEEEECCCEEEEEHHCCCCCCEEE
TPSMSILTEPPVAIVDKVVDQKGTREVAEAYLQFLYSDEGQELIADHYYRPRNEAVLEEH
CCCHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHH
RDVFPEVELFTIDDKFGGWQEAQDKHFADGGIFDQIYQPN
HHCCCCEEEEEEECCCCCCHHHCCCCCCCCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3158524; 2002055; 8346018; 9278503; 9298646; 8774726 [H]