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The map label for this gene is lysX

Identifier: 55981876

GI number: 55981876

Start: 1791734

End: 1792576

Strand: Reverse

Name: lysX

Synonym: TTHA1907

Alternate gene names: 55981876

Gene position: 1792576-1791734 (Counterclockwise)

Preceding gene: 55981877

Following gene: 55981875

Centisome position: 96.91

GC content: 67.62

Gene sequence:

>843_bases
ATGCTGGCCATCCTCTACGACCGCATCCGCCCCGACGAGCGGATGCTCTTTGAGCGGGCGGAGGCCCTGGGCCTCCCCTA
CAAGAAGGTCTACGTCCCCGCCCTCCCCATGGTCCTGGGGGAAAGGCCCAAGGAACTGGAAGGGGTTACGGTGGCCCTGG
AGCGGTGCGTGAGCCAGAGCCGGGGCCTCGCCGCCGCCCGCTACCTCACCGCCCTCGGCATCCCCGTGGTGAACCGCCCC
GAGGTCATAGAGGCCTGCGGGGACAAGTGGGCCACGAGCGTGGCCTTGGCCAAGGCGGGCCTTCCCCAGCCCAAGACCGC
CTTGGCCACGGACCGGGAGGAGGCTTTAAGGCTCATGGAGGCCTTCGGCTACCCCGTGGTGCTGAAGCCCGTGATCGGGA
GCTGGGGCCGCCTCCTCGCCAAGGTCACGGACCGGGCCGCGGCGGAGGCCCTTCTTGAGCACAAGGAGGTCCTGGGGGGG
TTTCAGCACCAGCTTTTTTACATCCAGGAGTACGTGGAGAAGCCCGGGCGGGACATCCGGGTCTTCGTGGTGGGGGAAAG
GGCCATCGCCGCCATCTACCGCAGAAGCGCCCACTGGATCACCAACACCGCCCGGGGCGGGCAGGCGGAGAACTGCCCCC
TCACCGAGGAGGTCGCCCGGCTTTCCGTGAAGGCGGCGGAGGCCGTTGGGGGCGGGGTGGTGGCCGTGGACCTCTTTGAG
TCGGAGCGGGGGCTTTTGGTCAACGAGGTGAACCACACCATGGAGTTCAAGAACTCCGTGCACACCACCGGGGTGGACAT
CCCGGGGGAAATTCTGAAGTACGCGTGGAGCCTAGCCTCATGA

Upstream 100 bases:

>100_bases
ACCCCACGCCGGCCTAGGCCGGCGTGGGGGCCCCGGCGGCGCCACCCCATGCCGGCCTGCGCCCCTGGCAGGGCCTGGCG
CCCTCTTTCCCGGAGGCTAG

Downstream 100 bases:

>100_bases
CCTTGCGGGAAGCGGTGGAGCGTTTGGTGGCGGCCTTGGCGCCCGAGGAGGTCTGGCTCTTCGGCTCTTGGGCCCGGGGG
GAGGCCGGGCCGGACTCGGA

Product: lysine biosynthesis protein LysX

Products: NA

Alternate protein names: AAA--lysW ligase lysX

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MLAILYDRIRPDERMLFERAEALGLPYKKVYVPALPMVLGERPKELEGVTVALERCVSQSRGLAAARYLTALGIPVVNRP
EVIEACGDKWATSVALAKAGLPQPKTALATDREEALRLMEAFGYPVVLKPVIGSWGRLLAKVTDRAAAEALLEHKEVLGG
FQHQLFYIQEYVEKPGRDIRVFVVGERAIAAIYRRSAHWITNTARGGQAENCPLTEEVARLSVKAAEAVGGGVVAVDLFE
SERGLLVNEVNHTMEFKNSVHTTGVDIPGEILKYAWSLAS

Sequences:

>Translated_280_residues
MLAILYDRIRPDERMLFERAEALGLPYKKVYVPALPMVLGERPKELEGVTVALERCVSQSRGLAAARYLTALGIPVVNRP
EVIEACGDKWATSVALAKAGLPQPKTALATDREEALRLMEAFGYPVVLKPVIGSWGRLLAKVTDRAAAEALLEHKEVLGG
FQHQLFYIQEYVEKPGRDIRVFVVGERAIAAIYRRSAHWITNTARGGQAENCPLTEEVARLSVKAAEAVGGGVVAVDLFE
SERGLLVNEVNHTMEFKNSVHTTGVDIPGEILKYAWSLAS
>Mature_280_residues
MLAILYDRIRPDERMLFERAEALGLPYKKVYVPALPMVLGERPKELEGVTVALERCVSQSRGLAAARYLTALGIPVVNRP
EVIEACGDKWATSVALAKAGLPQPKTALATDREEALRLMEAFGYPVVLKPVIGSWGRLLAKVTDRAAAEALLEHKEVLGG
FQHQLFYIQEYVEKPGRDIRVFVVGERAIAAIYRRSAHWITNTARGGQAENCPLTEEVARLSVKAAEAVGGGVVAVDLFE
SERGLLVNEVNHTMEFKNSVHTTGVDIPGEILKYAWSLAS

Specific function: Catalyzes the ATP-dependent binding between the amino group of alpha-aminoadipate (AAA) and the gamma-carboxyl group of 'Glu-54' of lysW

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Homo sapiens, GI164663810, Length=230, Percent_Identity=30.4347826086957, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI134254462, Length=230, Percent_Identity=29.5652173913043, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1787076, Length=217, Percent_Identity=32.258064516129, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LYSX_THET8 (Q5SH23)

Other databases:

- EMBL:   AB103032
- EMBL:   AP008226
- RefSeq:   YP_145173.1
- PDB:   1UC8
- PDB:   1UC9
- PDBsum:   1UC8
- PDBsum:   1UC9
- ProteinModelPortal:   Q5SH23
- SMR:   Q5SH23
- STRING:   Q5SH23
- GeneID:   3167971
- GenomeReviews:   AP008226_GR
- KEGG:   ttj:TTHA1907
- NMPDR:   fig|300852.3.peg.1518
- eggNOG:   COG0189
- HOGENOM:   HBG679591
- OMA:   MEFKNSV
- ProtClustDB:   CLSK2761981
- BioCyc:   TTHE300852:TTHA1907-MONOMER
- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011870
- InterPro:   IPR016185
- InterPro:   IPR004666
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- TIGRFAMs:   TIGR02144
- TIGRFAMs:   TIGR00768

Pfam domain/function: PF08443 RimK; SSF52440 PreATP-grasp-like

EC number: NA

Molecular weight: Translated: 30718; Mature: 30718

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAILYDRIRPDERMLFERAEALGLPYKKVYVPALPMVLGERPKELEGVTVALERCVSQS
CEEEEEHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
RGLAAARYLTALGIPVVNRPEVIEACGDKWATSVALAKAGLPQPKTALATDREEALRLME
CCHHHHHHHHHHCCCCCCCCHHHHHHCHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHH
AFGYPVVLKPVIGSWGRLLAKVTDRAAAEALLEHKEVLGGFQHQLFYIQEYVEKPGRDIR
HCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
VFVVGERAIAAIYRRSAHWITNTARGGQAENCPLTEEVARLSVKAAEAVGGGVVAVDLFE
EEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEC
SERGLLVNEVNHTMEFKNSVHTTGVDIPGEILKYAWSLAS
CCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MLAILYDRIRPDERMLFERAEALGLPYKKVYVPALPMVLGERPKELEGVTVALERCVSQS
CEEEEEHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
RGLAAARYLTALGIPVVNRPEVIEACGDKWATSVALAKAGLPQPKTALATDREEALRLME
CCHHHHHHHHHHCCCCCCCCHHHHHHCHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHH
AFGYPVVLKPVIGSWGRLLAKVTDRAAAEALLEHKEVLGGFQHQLFYIQEYVEKPGRDIR
HCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
VFVVGERAIAAIYRRSAHWITNTARGGQAENCPLTEEVARLSVKAAEAVGGGVVAVDLFE
EEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEC
SERGLLVNEVNHTMEFKNSVHTTGVDIPGEILKYAWSLAS
CCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12963379