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The map label for this gene is yfhQ [H]

Identifier: 55981867

GI number: 55981867

Start: 1784531

End: 1785508

Strand: Reverse

Name: yfhQ [H]

Synonym: TTHA1898

Alternate gene names: 55981867

Gene position: 1785508-1784531 (Counterclockwise)

Preceding gene: 55981868

Following gene: 55981863

Centisome position: 96.53

GC content: 71.78

Gene sequence:

>978_bases
GTGGAGGCCTGGCGGAAAGCCCTCCTCGCCTGGTACCGGGAAAACGCCCGCCCCCTCCCCTGGCGGGGGGAGAAGGACCC
TTACCGCGTCCTGGTCTCCGAGGTCCTTCTGCAGCAGACCCGGGTGGAGCAGGCCCTCCCCTATTACCGCCGCTTTCTGG
AGCGCTTTCCCACCCTGAAGGCCCTGGCCGCGGCTTCCCTGGAAGAGGTCCTTAGGGTCTGGCAGGGGGCGGGCTACTAC
CGGCGGGCGGAACACCTCCACCGCCTGGCCCGAAGCGTGGAGGAGCTTCCCCCGAGCTTCGCCGAGCTTCGGGGGCTTCC
TGGTCTCGGGCCTTACACCGCGGCGGCGGTGGCCTCCATCGCCTTCGGGGAGCGGGTGGCGGCGGTGGACGGGAACGTCC
GGAGGGTCCTCTCCCGCCTCTTCGCCCGGGAAAGCCCCAAGGAGAAGGAGCTTTTCGCCCTCGCCCAGGGCCTCCTCCCC
GAGGGCGTGGACCCGGGGGTGTGGAACCAGGCCCTCATGGAGCTCGGGGCCACGGTCTGCCTGCCGAAACGGCCCCGTTG
CGGGGCCTGCCCCCTAGGGGCCTTCTGCCGGGGGAAGGAGGCCCCCGGGCGCTACCCCGCGCCCAGGAAGCGCCGGGCGA
AGGAGGAGCGCCTCGTCGCCCTCGTCCTCCTCGGGCGGAAGGGGGTGCACCTGGAAAGGCTTGAGGGGCGCTTCCAGGGC
CTCTACGGCGTCCCCCTCTTTCCCCCTGAGGAGCTTCCCGGGCGGGAGGCGGCCTTCGGGGTGAGGTCTAGGCCCCTAGG
CGAGGTGCGCCACGCCCTCACCCACCGGAGGCTTCGCGTGGAGGTGCGGGGGGCCCTTTGGGAAGGGGAGGGGGAGGACC
CCTGGAAGAGGCCCCTACCCAAGCTCATGGAGAAGGTGCTCCGCAAGGCGCTTCCCCTCCTCGCTCATGCGGGCGTAGTC
CCCCTCCCGGACGCATAG

Upstream 100 bases:

>100_bases
TTGAAAAGGCCCTGAAGGAGGCGGGCATCCCCGTGGTGGCCGACCGCTGCCTCATGGTGGAGCACAAGCGCCTCTTCCGG
GGGCCCCTTCCCCTCTAGCC

Downstream 100 bases:

>100_bases
GCCCACGGCGTATAGCCCTTCCAGGCGCTTGAGGCGGAAGGTCTTTTCCTCCCACTCCTCGGGGTGGAAGGCCAGGTAGC
GCACCCGGTAGCCGGGGGTG

Product: A/G-specific adenine glycosylase MutY

Products: Adenine [C]

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MEAWRKALLAWYRENARPLPWRGEKDPYRVLVSEVLLQQTRVEQALPYYRRFLERFPTLKALAAASLEEVLRVWQGAGYY
RRAEHLHRLARSVEELPPSFAELRGLPGLGPYTAAAVASIAFGERVAAVDGNVRRVLSRLFARESPKEKELFALAQGLLP
EGVDPGVWNQALMELGATVCLPKRPRCGACPLGAFCRGKEAPGRYPAPRKRRAKEERLVALVLLGRKGVHLERLEGRFQG
LYGVPLFPPEELPGREAAFGVRSRPLGEVRHALTHRRLRVEVRGALWEGEGEDPWKRPLPKLMEKVLRKALPLLAHAGVV
PLPDA

Sequences:

>Translated_325_residues
MEAWRKALLAWYRENARPLPWRGEKDPYRVLVSEVLLQQTRVEQALPYYRRFLERFPTLKALAAASLEEVLRVWQGAGYY
RRAEHLHRLARSVEELPPSFAELRGLPGLGPYTAAAVASIAFGERVAAVDGNVRRVLSRLFARESPKEKELFALAQGLLP
EGVDPGVWNQALMELGATVCLPKRPRCGACPLGAFCRGKEAPGRYPAPRKRRAKEERLVALVLLGRKGVHLERLEGRFQG
LYGVPLFPPEELPGREAAFGVRSRPLGEVRHALTHRRLRVEVRGALWEGEGEDPWKRPLPKLMEKVLRKALPLLAHAGVV
PLPDA
>Mature_325_residues
MEAWRKALLAWYRENARPLPWRGEKDPYRVLVSEVLLQQTRVEQALPYYRRFLERFPTLKALAAASLEEVLRVWQGAGYY
RRAEHLHRLARSVEELPPSFAELRGLPGLGPYTAAAVASIAFGERVAAVDGNVRRVLSRLFARESPKEKELFALAQGLLP
EGVDPGVWNQALMELGATVCLPKRPRCGACPLGAFCRGKEAPGRYPAPRKRRAKEERLVALVLLGRKGVHLERLEGRFQG
LYGVPLFPPEELPGREAAFGVRSRPLGEVRHALTHRRLRVEVRGALWEGEGEDPWKRPLPKLMEKVLRKALPLLAHAGVV
PLPDA

Specific function: Involved in the GO system responsible for removing an oxidatively damaged form of guanine (7,8-dihydro-8-oxoguanine, 8- oxo-dGTP) from DNA and the nucleotide pool. 8-oxo-dGTP is inserted opposite dA and dC residues of template DNA with almost equal effici

COG id: COG1194

COG function: function code L; A/G-specific DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HhH domain [H]

Homologues:

Organism=Homo sapiens, GI6912520, Length=219, Percent_Identity=45.662100456621, Blast_Score=171, Evalue=7e-43,
Organism=Homo sapiens, GI115298648, Length=219, Percent_Identity=45.662100456621, Blast_Score=171, Evalue=7e-43,
Organism=Homo sapiens, GI115298654, Length=219, Percent_Identity=45.662100456621, Blast_Score=171, Evalue=7e-43,
Organism=Homo sapiens, GI115298652, Length=219, Percent_Identity=45.662100456621, Blast_Score=171, Evalue=7e-43,
Organism=Homo sapiens, GI115298650, Length=219, Percent_Identity=45.662100456621, Blast_Score=171, Evalue=7e-43,
Organism=Homo sapiens, GI190358497, Length=219, Percent_Identity=45.662100456621, Blast_Score=171, Evalue=8e-43,
Organism=Escherichia coli, GI1789331, Length=294, Percent_Identity=37.7551020408163, Blast_Score=179, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI17554540, Length=181, Percent_Identity=32.0441988950276, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR023170
- InterPro:   IPR005760
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00730 HhH-GPD [H]

EC number: 3.2.2.- [C]

Molecular weight: Translated: 36546; Mature: 36546

Theoretical pI: Translated: 10.62; Mature: 10.62

Prosite motif: PS00764 ENDONUCLEASE_III_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAWRKALLAWYRENARPLPWRGEKDPYRVLVSEVLLQQTRVEQALPYYRRFLERFPTLK
CCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
ALAAASLEEVLRVWQGAGYYRRAEHLHRLARSVEELPPSFAELRGLPGLGPYTAAAVASI
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHH
AFGERVAAVDGNVRRVLSRLFARESPKEKELFALAQGLLPEGVDPGVWNQALMELGATVC
HHHCHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCEE
LPKRPRCGACPLGAFCRGKEAPGRYPAPRKRRAKEERLVALVLLGRKGVHLERLEGRFQG
CCCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCC
LYGVPLFPPEELPGREAAFGVRSRPLGEVRHALTHRRLRVEVRGALWEGEGEDPWKRPLP
CCCCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHCHHH
KLMEKVLRKALPLLAHAGVVPLPDA
HHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MEAWRKALLAWYRENARPLPWRGEKDPYRVLVSEVLLQQTRVEQALPYYRRFLERFPTLK
CCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
ALAAASLEEVLRVWQGAGYYRRAEHLHRLARSVEELPPSFAELRGLPGLGPYTAAAVASI
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHH
AFGERVAAVDGNVRRVLSRLFARESPKEKELFALAQGLLPEGVDPGVWNQALMELGATVC
HHHCHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCEE
LPKRPRCGACPLGAFCRGKEAPGRYPAPRKRRAKEERLVALVLLGRKGVHLERLEGRFQG
CCCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCC
LYGVPLFPPEELPGREAAFGVRSRPLGEVRHALTHRRLRVEVRGALWEGEGEDPWKRPLP
CCCCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHCHHH
KLMEKVLRKALPLLAHAGVVPLPDA
HHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: 4Fe-4S Cluster [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Guanine-adenine mispair [C]

Specific reaction: Catalyzes the excision of adenine from a guanine-adenine mispair [C]

General reaction: Hydrolase; Glycosylases; Hydrolysing N-glycosyl compounds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8946165; 9384377 [H]