Click here to switch to the map view.

The map label for this gene is slpA

Identifier: 55981862

GI number: 55981862

Start: 1777608

End: 1780394

Strand: Reverse

Name: slpA

Synonym: TTHA1893

Alternate gene names: 55981862

Gene position: 1780394-1777608 (Counterclockwise)

Preceding gene: 55981863

Following gene: 55981861

Centisome position: 96.25

GC content: 67.17

Gene sequence:

>2787_bases
ATGAAGAAAAGGCTGGTCACACTACTGGCAGGGCTCTTGACCGTCCTCTCCATGGGGTTCGGTCTGGCCCAGTTCTCCGA
CGTGCCCGCCGGGCACTGGGCCAAGGAGGCCGTGGAGGCCCTGGCGGCCAAGGGCATCATCCTGGGCTTCCCGGACGGCA
CCTTCCGGGGCAACGAGAACCTCACCCGCTACCAGGCGGCCCTCCTCATCTACCGGCTCTTGCAGCAGATTGAGGAGGAG
CTGAAGACCCAGGGCACCTCCCCCACCATGGAGGCCCTGGCCCCCGAGGACCTCGAGGCCCTGAAGAACGCCGTCCAGGA
GCTCGCCGCCGAGCTCGCTTCCCTGGGCGTGCGGGTCTCCGCCCTGGAGGACAGCGCCGCCACCAAGGAGGACATCGCCC
GCCTCGAGGCGATGATCGCCGAGCTCAAGGCCCAGCCCATGCCCGAGCCCGGCATGGACCAGGCGGCCCTTAAGGACCTC
ATGGACCGGGTGGAGGCCGCCTCCATCGCCGCCGACACCGCCCTCGCCCAGGCCCAGCAGCTCGCCGAGCGGCTGGACGC
CCTGGCTCAGGACGTGGAGGGCGTGAAGGGCGACCTGGCCGGGCTTAGGAGCCAGGTGGAGGCCAACGCCGACGCCATCC
AGGCCCTGAACGAGCTCGCCGTCCTCCTGAACCAGGACGTCCTCTCCCTCCAGGACCGGGTCACCGCCCTGGAGAAGATG
GTCTCCGGCGGCCAGGAGCTCCCCGACCTGGAGCAGTTCGCCACCAAGGAGGACGTGGCCGCCGTCCAGGAGTTCGCCGC
CGCCCTGCGCTCCGACCTGGTGGGCCTCTCCGACAAGGTCTCCAAGCTGGAAGAACAGGTGGCCGAGCTCAACAAGGTGC
GCTACTCCATCTCCGGTAGCCTCTCCGCCACCTACGGCACGGTGGTGACCGACACCGGCACCAACTTTGACATTGACCGC
CTCTTCCCGGGCAACGCCTTCTCCACGGGGACCTACGGCTCCTTCTCCTCCAGCGTCCAGGCGGGGGACAGCAACCAGGG
CAACATCTCGGGGGGCTCCGCTTCCCTCACCTTCGGCGTGAAGGTGGCCCAGCCCGGCACGAGCGGGGTGAACGTCTCCG
AGGCCAGCGCCACCCTCCAGGTGCCCGCGGCTTTCGGGACCGCTTACACCTCTGCTCCCACCATCCGCCTCAACGCCGCG
AGCGTCAAGGGCAACGTGGACGGCCAGGCCTTCAGCGTGGTCTACAGCCGGGCGGTGAGCAGCTTCAAGTTCAACGACTA
CCTCTTCGCCAACGACAACGACTCTGAGCCGGCCAACCCCCGCCAGGGTATGGTGGCCACCTTCTCCGCCACCAAGTTCC
CCCTTGCCCCCGAGGTGACCGTGGTGGCCGGGGTGGCCGGACCTGATGCTACTAAGGATACTGCTCCTGCCCTAAACGGC
AACTACTTCGGCATCCGCACCGCCGTCAAGCCCTTCTCCGCCCTCAACCTGGCCCTGAACTACGCCACCAACCTGGGCAA
CCGCTCCGCCATTGGCGTGGACGGCGGGCTGGAGCTCGGCCCCGCCAAGCTCTCCGGCCTCTGGGTCTCCTCCCAGACCC
CCGGGAGCCCCTTCGCCGACTTCTTTGACAACACCCTCTCCGACTGGGCGTATTACGCCCAGGCCGAGGCGAAGCTCGGG
CCTCTTTCCCTCTCCGCCAACTACCACGCCGTGGACCCGCAGTACGCCGACGGCCAGGCGGGCATGTCCGAGAACGAGGA
CACCACCTACTACGGCGGTGAGAAGGCAGGCGCTCCCTACGGGGCGGACACCCGCGGCCTGGGCGTCTCCGCCAGCGTGG
GCTTCGGCCCCGTTACCCTCAAGGGCTACGCCGAGAGCGAGGGGGACTACAACCTGGCTCCGGGCAGCGTCAACGACGCT
TGGGGCGTGGCCGCTACCTTGGGGTCCTTCCGGGGATTCAGCCTGACGGGCTTCTACAACGCCGCCTACACCGGTGGTAA
CGGTTACTTCTCCCTTACCACCGCTGTGGACGCCATTGCTCCGGGTGTGACTTACTACTACACCATTGAGAACCAGAAGT
ACTCCTCCAGCTGGGGCGTGCGGGTGGCCCACGACGGCAAGGCCGAGGACGCCCTAATCCCCACCCTCAACCTCACCGCC
CAGTACGCCACCTACTACGTCTCCGGCCACACGGACATCCAGGTCTACGCCGACCTGGCCAAGCCCTTCAAGCTGGCCAT
CCTGAGCCTCTCCCCGGGCTTCCGCTACCACAGCTTCGCTGGTGCGGGTAGCGCCCCGACCTACACCACCCTGAAGGGCG
GGGTCCAGGTTTCCACCGATCCCCTCCTCTTCGGTCTCAGCCTGGACGGGGCGGTCTCCTACCGCCGGACGCAGTACACC
AACAACCCCTCCAACGTGACCACCTACGAGCTCTACTACCGCGCCGGGGTGAAGCTCCAGGACTTCCTGGCCCCCAAGCT
CAACTTCAGCGTGGCCTACGCCCACTACGAGGGCGATCAGCTTGCCGGCACCGGCCTCCCCGTGGTGGGGTCCGGCAACC
AGGCCTTCAACTTCGCCCGCGACCGGGTCTACCGGAGCCCCGATCCCATCGCTGCTCCTTGGCTGGCCACTCCGGGCACC
CAGGCGGGCAAGCTGGACGGCTTCTACATTGAGGCCAAGTACTACGACCTCACCGTGGCCTACGGGGAGTTCGTCCTGGA
TGACCTCAACGGGACCAACCCCAACTTCGGCCGGGGCTTCAAGATCAGCTACACCGTGAAGTTCTAA

Upstream 100 bases:

>100_bases
GGGGGTTTTCCCCGCACCGGGCGGACCTGGTCGGAGAGGAAACGCGGCAACTCGCCCGTCTCGGGTTCCCGCCCACGACC
CTTAAGGGGGTGTGAGGCAT

Downstream 100 bases:

>100_bases
CCCTGGCGGTCCCGCCCCCGCCCCCCCAAAGGGGGCGGGGGTTTTTGTTCTCCCCCTTCCTTTGGGGAGGCGAGGGGGCC
CCTATACTGGGTGAGGATGA

Product: S-layer P100 protein)

Products: NA

Alternate protein names: P100 protein; Surface layer protein

Number of amino acids: Translated: 928; Mature: 928

Protein sequence:

>928_residues
MKKRLVTLLAGLLTVLSMGFGLAQFSDVPAGHWAKEAVEALAAKGIILGFPDGTFRGNENLTRYQAALLIYRLLQQIEEE
LKTQGTSPTMEALAPEDLEALKNAVQELAAELASLGVRVSALEDSAATKEDIARLEAMIAELKAQPMPEPGMDQAALKDL
MDRVEAASIAADTALAQAQQLAERLDALAQDVEGVKGDLAGLRSQVEANADAIQALNELAVLLNQDVLSLQDRVTALEKM
VSGGQELPDLEQFATKEDVAAVQEFAAALRSDLVGLSDKVSKLEEQVAELNKVRYSISGSLSATYGTVVTDTGTNFDIDR
LFPGNAFSTGTYGSFSSSVQAGDSNQGNISGGSASLTFGVKVAQPGTSGVNVSEASATLQVPAAFGTAYTSAPTIRLNAA
SVKGNVDGQAFSVVYSRAVSSFKFNDYLFANDNDSEPANPRQGMVATFSATKFPLAPEVTVVAGVAGPDATKDTAPALNG
NYFGIRTAVKPFSALNLALNYATNLGNRSAIGVDGGLELGPAKLSGLWVSSQTPGSPFADFFDNTLSDWAYYAQAEAKLG
PLSLSANYHAVDPQYADGQAGMSENEDTTYYGGEKAGAPYGADTRGLGVSASVGFGPVTLKGYAESEGDYNLAPGSVNDA
WGVAATLGSFRGFSLTGFYNAAYTGGNGYFSLTTAVDAIAPGVTYYYTIENQKYSSSWGVRVAHDGKAEDALIPTLNLTA
QYATYYVSGHTDIQVYADLAKPFKLAILSLSPGFRYHSFAGAGSAPTYTTLKGGVQVSTDPLLFGLSLDGAVSYRRTQYT
NNPSNVTTYELYYRAGVKLQDFLAPKLNFSVAYAHYEGDQLAGTGLPVVGSGNQAFNFARDRVYRSPDPIAAPWLATPGT
QAGKLDGFYIEAKYYDLTVAYGEFVLDDLNGTNPNFGRGFKISYTVKF

Sequences:

>Translated_928_residues
MKKRLVTLLAGLLTVLSMGFGLAQFSDVPAGHWAKEAVEALAAKGIILGFPDGTFRGNENLTRYQAALLIYRLLQQIEEE
LKTQGTSPTMEALAPEDLEALKNAVQELAAELASLGVRVSALEDSAATKEDIARLEAMIAELKAQPMPEPGMDQAALKDL
MDRVEAASIAADTALAQAQQLAERLDALAQDVEGVKGDLAGLRSQVEANADAIQALNELAVLLNQDVLSLQDRVTALEKM
VSGGQELPDLEQFATKEDVAAVQEFAAALRSDLVGLSDKVSKLEEQVAELNKVRYSISGSLSATYGTVVTDTGTNFDIDR
LFPGNAFSTGTYGSFSSSVQAGDSNQGNISGGSASLTFGVKVAQPGTSGVNVSEASATLQVPAAFGTAYTSAPTIRLNAA
SVKGNVDGQAFSVVYSRAVSSFKFNDYLFANDNDSEPANPRQGMVATFSATKFPLAPEVTVVAGVAGPDATKDTAPALNG
NYFGIRTAVKPFSALNLALNYATNLGNRSAIGVDGGLELGPAKLSGLWVSSQTPGSPFADFFDNTLSDWAYYAQAEAKLG
PLSLSANYHAVDPQYADGQAGMSENEDTTYYGGEKAGAPYGADTRGLGVSASVGFGPVTLKGYAESEGDYNLAPGSVNDA
WGVAATLGSFRGFSLTGFYNAAYTGGNGYFSLTTAVDAIAPGVTYYYTIENQKYSSSWGVRVAHDGKAEDALIPTLNLTA
QYATYYVSGHTDIQVYADLAKPFKLAILSLSPGFRYHSFAGAGSAPTYTTLKGGVQVSTDPLLFGLSLDGAVSYRRTQYT
NNPSNVTTYELYYRAGVKLQDFLAPKLNFSVAYAHYEGDQLAGTGLPVVGSGNQAFNFARDRVYRSPDPIAAPWLATPGT
QAGKLDGFYIEAKYYDLTVAYGEFVLDDLNGTNPNFGRGFKISYTVKF
>Mature_928_residues
MKKRLVTLLAGLLTVLSMGFGLAQFSDVPAGHWAKEAVEALAAKGIILGFPDGTFRGNENLTRYQAALLIYRLLQQIEEE
LKTQGTSPTMEALAPEDLEALKNAVQELAAELASLGVRVSALEDSAATKEDIARLEAMIAELKAQPMPEPGMDQAALKDL
MDRVEAASIAADTALAQAQQLAERLDALAQDVEGVKGDLAGLRSQVEANADAIQALNELAVLLNQDVLSLQDRVTALEKM
VSGGQELPDLEQFATKEDVAAVQEFAAALRSDLVGLSDKVSKLEEQVAELNKVRYSISGSLSATYGTVVTDTGTNFDIDR
LFPGNAFSTGTYGSFSSSVQAGDSNQGNISGGSASLTFGVKVAQPGTSGVNVSEASATLQVPAAFGTAYTSAPTIRLNAA
SVKGNVDGQAFSVVYSRAVSSFKFNDYLFANDNDSEPANPRQGMVATFSATKFPLAPEVTVVAGVAGPDATKDTAPALNG
NYFGIRTAVKPFSALNLALNYATNLGNRSAIGVDGGLELGPAKLSGLWVSSQTPGSPFADFFDNTLSDWAYYAQAEAKLG
PLSLSANYHAVDPQYADGQAGMSENEDTTYYGGEKAGAPYGADTRGLGVSASVGFGPVTLKGYAESEGDYNLAPGSVNDA
WGVAATLGSFRGFSLTGFYNAAYTGGNGYFSLTTAVDAIAPGVTYYYTIENQKYSSSWGVRVAHDGKAEDALIPTLNLTA
QYATYYVSGHTDIQVYADLAKPFKLAILSLSPGFRYHSFAGAGSAPTYTTLKGGVQVSTDPLLFGLSLDGAVSYRRTQYT
NNPSNVTTYELYYRAGVKLQDFLAPKLNFSVAYAHYEGDQLAGTGLPVVGSGNQAFNFARDRVYRSPDPIAAPWLATPGT
QAGKLDGFYIEAKYYDLTVAYGEFVLDDLNGTNPNFGRGFKISYTVKF

Specific function: The S-layer is a paracrystalline mono-layered assembly of proteins which coat the surface of bacteria

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted, cell wall, S-layer. Note=This bacterium is covered by a S-layer with hexagonal symmetry (By similarity)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SLH (S-layer homology) domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SLAP1_THET8 (Q5SH37)

Other databases:

- EMBL:   AP008226
- RefSeq:   YP_145159.1
- ProteinModelPortal:   Q5SH37
- STRING:   Q5SH37
- GeneID:   3168016
- GenomeReviews:   AP008226_GR
- KEGG:   ttj:TTHA1893
- NMPDR:   fig|300852.3.peg.1504
- HOGENOM:   HBG449923
- OMA:   LFANDND
- ProtClustDB:   CLSK751157
- BioCyc:   TTHE300852:TTHA1893-MONOMER
- GO:   GO:0030115
- InterPro:   IPR001119

Pfam domain/function: PF00395 SLH

EC number: NA

Molecular weight: Translated: 98038; Mature: 98038

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: PS51272 SLH; PS01072 SLH_DOMAIN ; PS01156 TONB_DEPENDENT_REC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRLVTLLAGLLTVLSMGFGLAQFSDVPAGHWAKEAVEALAAKGIILGFPDGTFRGNEN
CCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHCCEEEECCCCCCCCCCC
LTRYQAALLIYRLLQQIEEELKTQGTSPTMEALAPEDLEALKNAVQELAAELASLGVRVS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHCCEEEE
ALEDSAATKEDIARLEAMIAELKAQPMPEPGMDQAALKDLMDRVEAASIAADTALAQAQQ
ECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAERLDALAQDVEGVKGDLAGLRSQVEANADAIQALNELAVLLNQDVLSLQDRVTALEKM
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSGGQELPDLEQFATKEDVAAVQEFAAALRSDLVGLSDKVSKLEEQVAELNKVRYSISGS
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHEEEECCC
LSATYGTVVTDTGTNFDIDRLFPGNAFSTGTYGSFSSSVQAGDSNQGNISGGSASLTFGV
CEEEECEEEECCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEE
KVAQPGTSGVNVSEASATLQVPAAFGTAYTSAPTIRLNAASVKGNVDGQAFSVVYSRAVS
EEECCCCCCCEEECCCCEEEECHHHCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHH
SFKFNDYLFANDNDSEPANPRQGMVATFSATKFPLAPEVTVVAGVAGPDATKDTAPALNG
CCCCCCEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEECCCCCCCCCCCCCCCC
NYFGIRTAVKPFSALNLALNYATNLGNRSAIGVDGGLELGPAKLSGLWVSSQTPGSPFAD
CEEEEHHHHCCHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCEEEEEEEECCCCCCCHHH
FFDNTLSDWAYYAQAEAKLGPLSLSANYHAVDPQYADGQAGMSENEDTTYYGGEKAGAPY
HHHHHHHHHHHHHHHCCCCCCEEECCCEEECCCCCCCCCCCCCCCCCCEEECCCCCCCCC
GADTRGLGVSASVGFGPVTLKGYAESEGDYNLAPGSVNDAWGVAATLGSFRGFSLTGFYN
CCCCCCCCEEECCCCCCEEEEEEECCCCCCEECCCCCCCHHHHHHHHHCCCCEEEEEEEE
AAYTGGNGYFSLTTAVDAIAPGVTYYYTIENQKYSSSWGVRVAHDGKAEDALIPTLNLTA
EEEECCCCEEEHHHHHHHHCCCEEEEEEECCCEECCCCCEEEEECCCCCCCEECEEECEE
QYATYYVSGHTDIQVYADLAKPFKLAILSLSPGFRYHSFAGAGSAPTYTTLKGGVQVSTD
EEEEEEEECCCCEEEEECCCCCEEEEEEEECCCCCEECCCCCCCCCCEEEECCCEEECCC
PLLFGLSLDGAVSYRRTQYTNNPSNVTTYELYYRAGVKLQDFLAPKLNFSVAYAHYEGDQ
CEEEEEECCCCCCCHHHCCCCCCCCCEEEEEEEECCCCHHHHHCCCCCEEEEEEEECCCC
LAGTGLPVVGSGNQAFNFARDRVYRSPDPIAAPWLATPGTQAGKLDGFYIEAKYYDLTVA
CCCCCCCEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEEE
YGEFVLDDLNGTNPNFGRGFKISYTVKF
ECHHHEECCCCCCCCCCCCEEEEEEEEC
>Mature Secondary Structure
MKKRLVTLLAGLLTVLSMGFGLAQFSDVPAGHWAKEAVEALAAKGIILGFPDGTFRGNEN
CCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHCCEEEECCCCCCCCCCC
LTRYQAALLIYRLLQQIEEELKTQGTSPTMEALAPEDLEALKNAVQELAAELASLGVRVS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHCCEEEE
ALEDSAATKEDIARLEAMIAELKAQPMPEPGMDQAALKDLMDRVEAASIAADTALAQAQQ
ECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAERLDALAQDVEGVKGDLAGLRSQVEANADAIQALNELAVLLNQDVLSLQDRVTALEKM
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSGGQELPDLEQFATKEDVAAVQEFAAALRSDLVGLSDKVSKLEEQVAELNKVRYSISGS
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHEEEECCC
LSATYGTVVTDTGTNFDIDRLFPGNAFSTGTYGSFSSSVQAGDSNQGNISGGSASLTFGV
CEEEECEEEECCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEE
KVAQPGTSGVNVSEASATLQVPAAFGTAYTSAPTIRLNAASVKGNVDGQAFSVVYSRAVS
EEECCCCCCCEEECCCCEEEECHHHCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHH
SFKFNDYLFANDNDSEPANPRQGMVATFSATKFPLAPEVTVVAGVAGPDATKDTAPALNG
CCCCCCEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEECCCCCCCCCCCCCCCC
NYFGIRTAVKPFSALNLALNYATNLGNRSAIGVDGGLELGPAKLSGLWVSSQTPGSPFAD
CEEEEHHHHCCHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCEEEEEEEECCCCCCCHHH
FFDNTLSDWAYYAQAEAKLGPLSLSANYHAVDPQYADGQAGMSENEDTTYYGGEKAGAPY
HHHHHHHHHHHHHHHCCCCCCEEECCCEEECCCCCCCCCCCCCCCCCCEEECCCCCCCCC
GADTRGLGVSASVGFGPVTLKGYAESEGDYNLAPGSVNDAWGVAATLGSFRGFSLTGFYN
CCCCCCCCEEECCCCCCEEEEEEECCCCCCEECCCCCCCHHHHHHHHHCCCCEEEEEEEE
AAYTGGNGYFSLTTAVDAIAPGVTYYYTIENQKYSSSWGVRVAHDGKAEDALIPTLNLTA
EEEECCCCEEEHHHHHHHHCCCEEEEEEECCCEECCCCCEEEEECCCCCCCEECEEECEE
QYATYYVSGHTDIQVYADLAKPFKLAILSLSPGFRYHSFAGAGSAPTYTTLKGGVQVSTD
EEEEEEEECCCCEEEEECCCCCEEEEEEEECCCCCEECCCCCCCCCCEEEECCCEEECCC
PLLFGLSLDGAVSYRRTQYTNNPSNVTTYELYYRAGVKLQDFLAPKLNFSVAYAHYEGDQ
CEEEEEECCCCCCCHHHCCCCCCCCCEEEEEEEECCCCHHHHHCCCCCEEEEEEEECCCC
LAGTGLPVVGSGNQAFNFARDRVYRSPDPIAAPWLATPGTQAGKLDGFYIEAKYYDLTVA
CCCCCCCEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEEE
YGEFVLDDLNGTNPNFGRGFKISYTVKF
ECHHHEECCCCCCCCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA