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The map label for this gene is purF [H]

Identifier: 55981489

GI number: 55981489

Start: 1445013

End: 1446404

Strand: Direct

Name: purF [H]

Synonym: TTHA1520

Alternate gene names: 55981489

Gene position: 1445013-1446404 (Clockwise)

Preceding gene: 55981488

Following gene: 55981493

Centisome position: 78.12

GC content: 70.19

Gene sequence:

>1392_bases
ATGGATAAGCCCCAGGAGGAGTGCGGCATCCTCGGGCTTTGGAGCGAAGGCCCCGTGGACGTGGCGGGGCTTCTCCACCT
CGGCCTCCTCGCCCTGCAGCACAGGGGCCAGGAGGCGGCGGGGATGGCGGTCACGGACGGCAAAGCGTTCCTGGTGGAAA
AGGACCTGGGCCTCGTGAACCAGGTCTTCACCGAGGAGCGCCTGGGGAAGCTCCGCCTTCCGGAGGCCCGCCTGGGCATC
GCCCACACCCGCTACTCCACCACGGGCTCCAACCTGCGCATCAACGCCCAGCCCCTCACCGCCCGCACCGCCCACGGGGT
CTTGGCCATCGCCCACAACGGCAACTTCACCAACGCCAAGCTCCTCCGCGACCGGCTCCTCCTGGAGGGGGCCACCTTCC
AGAGCACCTCGGACACCGAGGTGATGCTCCTCCTCCTCGCCCGCATGGGCCACCTCCCCCTCCCCGAGGCCGCCGCCCAG
GCCATGCGGCTCCTTCAGGGGGGGTATTCCATCCTCCTCATGAACCGCAAGACCCTCCTCGCCCTCCGCGACCCCCACGG
GGTGAGGCCCCTCGTCCTCGGCAAGGCCCCCTGGGGCTACGCCTTCGCCTCCGAGCCCCCGGCCCTCCTCCTCATGGGGG
CGGAGTACGTGCGGGACGTGCGCCCCGGGGAGGTGGTCTGGGTGGAGGAGGGAAGGCTCCAAAGCCTCCAGGCCCTCCCC
CCAGAGCCCGCCCCCTGCGCTTTTGAGTGGATCTACTTCGCCCGGCCCGACAGCGTTCTAGACGGCACCGAGGCCTACGA
GGCCCGGGTGAGGATGGGGATGGAGCTCTTCCGGGAGGCCCCCGCAGAGGCCGACCTCGTGGTCCCCGTCCCCGACTCGG
GCATCGGGGCCGCCGTGGGCTACGCCAAGGCGAGCGGGCTCCCCCTGGAGTTCGGCCTCTACAAGAACCCCTACGCCGGG
CGCACCTTCATCCAGCCCACCCAGGAGCTCCGCGACCTGAAGACGAGGCTCAAGCTCTCCCCCACGGGGGCGGTGAAGGG
CAAGCGGGTGGTCCTGGTGGACGACTCCATCGTCCGGGGCACCACGAGCCGCCGCATCGTGCGCATGCTCAAGGAGGCGG
GGGCCCTCGAGGTCCACTTCCGCGTCTCTAGCCCCCCCATCCGCTTCCCCTGCTACTACGGCATAGACACCGCGGCCCGC
AAGGAGCTCATCGCTGCGGAGAAGAGCGTGGAGGAGATCCAAGCCTACATCGGGGCGGACTCTTTGGCCTTCCTCTCGGA
GGAGGGGGTCAAGCGGGCCATCGGGCGGCCCGTCTGCCTCGCCTGCTTCAACGGGCGCTACCCGGCGGGGGTGCCCGAGG
AGGGGGAAAAGCTCGCCCTAGAGCTTCTCTAG

Upstream 100 bases:

>100_bases
GGGGGGAAGAGCCTCACGGTCCTCACCCCGGGGGGTGTCCTAGAGTGGAGCCTGGAGGAACTGCTTTCCGCCTGGAAAGC
GCCCCTCAGGGAGGTGCTGG

Downstream 100 bases:

>100_bases
GACGAAGACCCGGGGGTAGACGCCCCCCTGCTCCACCACCCGGGCGTGGCGGAGGGCGAAGCCCGGGGGCAGGGCCCGCT
TGAGGAGGGCGGGGCGCAGG

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MDKPQEECGILGLWSEGPVDVAGLLHLGLLALQHRGQEAAGMAVTDGKAFLVEKDLGLVNQVFTEERLGKLRLPEARLGI
AHTRYSTTGSNLRINAQPLTARTAHGVLAIAHNGNFTNAKLLRDRLLLEGATFQSTSDTEVMLLLLARMGHLPLPEAAAQ
AMRLLQGGYSILLMNRKTLLALRDPHGVRPLVLGKAPWGYAFASEPPALLLMGAEYVRDVRPGEVVWVEEGRLQSLQALP
PEPAPCAFEWIYFARPDSVLDGTEAYEARVRMGMELFREAPAEADLVVPVPDSGIGAAVGYAKASGLPLEFGLYKNPYAG
RTFIQPTQELRDLKTRLKLSPTGAVKGKRVVLVDDSIVRGTTSRRIVRMLKEAGALEVHFRVSSPPIRFPCYYGIDTAAR
KELIAAEKSVEEIQAYIGADSLAFLSEEGVKRAIGRPVCLACFNGRYPAGVPEEGEKLALELL

Sequences:

>Translated_463_residues
MDKPQEECGILGLWSEGPVDVAGLLHLGLLALQHRGQEAAGMAVTDGKAFLVEKDLGLVNQVFTEERLGKLRLPEARLGI
AHTRYSTTGSNLRINAQPLTARTAHGVLAIAHNGNFTNAKLLRDRLLLEGATFQSTSDTEVMLLLLARMGHLPLPEAAAQ
AMRLLQGGYSILLMNRKTLLALRDPHGVRPLVLGKAPWGYAFASEPPALLLMGAEYVRDVRPGEVVWVEEGRLQSLQALP
PEPAPCAFEWIYFARPDSVLDGTEAYEARVRMGMELFREAPAEADLVVPVPDSGIGAAVGYAKASGLPLEFGLYKNPYAG
RTFIQPTQELRDLKTRLKLSPTGAVKGKRVVLVDDSIVRGTTSRRIVRMLKEAGALEVHFRVSSPPIRFPCYYGIDTAAR
KELIAAEKSVEEIQAYIGADSLAFLSEEGVKRAIGRPVCLACFNGRYPAGVPEEGEKLALELL
>Mature_463_residues
MDKPQEECGILGLWSEGPVDVAGLLHLGLLALQHRGQEAAGMAVTDGKAFLVEKDLGLVNQVFTEERLGKLRLPEARLGI
AHTRYSTTGSNLRINAQPLTARTAHGVLAIAHNGNFTNAKLLRDRLLLEGATFQSTSDTEVMLLLLARMGHLPLPEAAAQ
AMRLLQGGYSILLMNRKTLLALRDPHGVRPLVLGKAPWGYAFASEPPALLLMGAEYVRDVRPGEVVWVEEGRLQSLQALP
PEPAPCAFEWIYFARPDSVLDGTEAYEARVRMGMELFREAPAEADLVVPVPDSGIGAAVGYAKASGLPLEFGLYKNPYAG
RTFIQPTQELRDLKTRLKLSPTGAVKGKRVVLVDDSIVRGTTSRRIVRMLKEAGALEVHFRVSSPPIRFPCYYGIDTAAR
KELIAAEKSVEEIQAYIGADSLAFLSEEGVKRAIGRPVCLACFNGRYPAGVPEEGEKLALELL

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=507, Percent_Identity=39.8422090729783, Blast_Score=351, Evalue=1e-96,
Organism=Escherichia coli, GI1788651, Length=442, Percent_Identity=41.6289592760181, Blast_Score=301, Evalue=4e-83,
Organism=Escherichia coli, GI1790167, Length=219, Percent_Identity=33.3333333333333, Blast_Score=80, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17554892, Length=480, Percent_Identity=38.75, Blast_Score=311, Evalue=5e-85,
Organism=Saccharomyces cerevisiae, GI6323958, Length=451, Percent_Identity=38.1374722838137, Blast_Score=263, Evalue=6e-71,
Organism=Drosophila melanogaster, GI24659598, Length=491, Percent_Identity=40.9368635437882, Blast_Score=357, Evalue=7e-99,
Organism=Drosophila melanogaster, GI24659604, Length=470, Percent_Identity=41.4893617021277, Blast_Score=349, Evalue=2e-96,
Organism=Drosophila melanogaster, GI28573187, Length=495, Percent_Identity=40, Blast_Score=348, Evalue=3e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 50389; Mature: 50389

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKPQEECGILGLWSEGPVDVAGLLHLGLLALQHRGQEAAGMAVTDGKAFLVEKDLGLVN
CCCCCHHCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEECCHHHHH
QVFTEERLGKLRLPEARLGIAHTRYSTTGSNLRINAQPLTARTAHGVLAIAHNGNFTNAK
HHHHHHHHCCCCCCHHHHCEEEEEEECCCCCEEEECCCCEEECCCCEEEEEECCCCCHHH
LLRDRLLLEGATFQSTSDTEVMLLLLARMGHLPLPEAAAQAMRLLQGGYSILLMNRKTLL
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEECCCEEE
ALRDPHGVRPLVLGKAPWGYAFASEPPALLLMGAEYVRDVRPGEVVWVEEGRLQSLQALP
EEECCCCCCEEEEECCCCCCCCCCCCCEEEEECHHHHHCCCCCCEEEEECCCHHHHHCCC
PEPAPCAFEWIYFARPDSVLDGTEAYEARVRMGMELFREAPAEADLVVPVPDSGIGAAVG
CCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHH
YAKASGLPLEFGLYKNPYAGRTFIQPTQELRDLKTRLKLSPTGAVKGKRVVLVDDSIVRG
HHHCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHEECCCCCCCCCCEEEEECCCHHCC
TTSRRIVRMLKEAGALEVHFRVSSPPIRFPCYYGIDTAARKELIAAEKSVEEIQAYIGAD
CHHHHHHHHHHHCCCEEEEEEECCCCEECCEEECCCHHHHHHHHHHHHHHHHHHHHHCCC
SLAFLSEEGVKRAIGRPVCLACFNGRYPAGVPEEGEKLALELL
HHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEEEC
>Mature Secondary Structure
MDKPQEECGILGLWSEGPVDVAGLLHLGLLALQHRGQEAAGMAVTDGKAFLVEKDLGLVN
CCCCCHHCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEECCHHHHH
QVFTEERLGKLRLPEARLGIAHTRYSTTGSNLRINAQPLTARTAHGVLAIAHNGNFTNAK
HHHHHHHHCCCCCCHHHHCEEEEEEECCCCCEEEECCCCEEECCCCEEEEEECCCCCHHH
LLRDRLLLEGATFQSTSDTEVMLLLLARMGHLPLPEAAAQAMRLLQGGYSILLMNRKTLL
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEECCCEEE
ALRDPHGVRPLVLGKAPWGYAFASEPPALLLMGAEYVRDVRPGEVVWVEEGRLQSLQALP
EEECCCCCCEEEEECCCCCCCCCCCCCEEEEECHHHHHCCCCCCEEEEECCCHHHHHCCC
PEPAPCAFEWIYFARPDSVLDGTEAYEARVRMGMELFREAPAEADLVVPVPDSGIGAAVG
CCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHH
YAKASGLPLEFGLYKNPYAGRTFIQPTQELRDLKTRLKLSPTGAVKGKRVVLVDDSIVRG
HHHCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHEECCCCCCCCCCEEEEECCCHHCC
TTSRRIVRMLKEAGALEVHFRVSSPPIRFPCYYGIDTAARKELIAAEKSVEEIQAYIGAD
CHHHHHHHHHHHCCCEEEEEEECCCCEECCEEECCCHHHHHHHHHHHHHHHHHHHHHCCC
SLAFLSEEGVKRAIGRPVCLACFNGRYPAGVPEEGEKLALELL
HHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6411717; 3036807; 9384377; 6411716; 6094545; 3090047; 8197456; 9271502 [H]