BacMap

Definition	Thermus thermophilus HB8 chromosome, complete genome.
Accession	NC_006461
Length	1,849,742

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The map label for this gene is paaF [C]

Identifier: 55981403

GI number: 55981403

Start: 1362013

End: 1362828

Strand: Reverse

Name: paaF [C]

Synonym: TTHA1434

Alternate gene names: 55981403

Gene position: 1362828-1362013 (Counterclockwise)

Preceding gene: 55981404

Following gene: 55981402

Centisome position: 73.68

GC content: 69.73

Gene sequence:

>816_bases
ATGGAGCACGAGCACGAACACGAGTTCGTCCTGGAGATCCCGGAGTTTGAGCACCTCAGCTACGAGGTGGAGGAGGGCAT
CGCCCTCGTGACCCTGAAGCGGCCCGAGGCCCTAAACGCCCTCTCCCAGAGCCTCCTGGAGGAGCTCGCCGAGATCCCCG
AGCTGGTCCAGCAGGACCCCGAGGTCCGGGCGGTCATCTTCACCGGGGAAGGGAAGGCCTTCGCCGCAGGGGCCGACCTG
AAGGAGATCGCGGCCATCAAGGACCCCTTCATGGGCCGGGAGTACGCCCTCTTCGGCCAGCGGGTCTTCGCCGAGATCGC
CGCCCTCCCCGTCCCCACCATCGCCGCCATCAACGGCTACGCCCTGGGCGGGGGCCTGGAGCTCGCCCTGGCCTGCGACC
TCCGGGTGGCGGCCAAGACCGCCAAGCTGGGCCTCCCCGAGGTGGGCCTCGGCCTCATCCCCGGCTTCGGAGGCACCCAG
CGCCTGCCCCGCCTCATCGGCCGGGGGAGGGCCTTGGACCTCATCTTCACCGGGCGGCACGTGGACGCGGAGGAGGCCCT
TTTCCTCGGGCTCGTGAACCGGGTGGCGGAGGACGCCCTGGAGGAGGCCAAGAAGCTCGCCCAAAAGATCTTGAAGAACG
CCCCCATCGCCTTGGCCCTGGCCAAGGAGAGCGTGGTCCGGGGCGAGGGCCTGGACCTGGCCGAGGCCCTGGAAATCGAG
GCCGACCTCTTCGGCTACGCCGCCGCCACCGAGGACATGAAGGAGGGGGTGCGGGCCTTCCTGGAGAAACGCCCCCCGAG
CTTCAAGGGCGAGTAG

Upstream 100 bases:

>100_bases
CCGCATCGGCGATCCGGAGCTCGCTCCCCCTGAGGTGCTCCAAGAGGGCGTTCGGCGTATGGTGGAGGTGGCCCTCGAGG
CCGTCCTGGAGGTTTGAACT

Downstream 100 bases:

>100_bases
GGCCGAAGCCCTTTCCTCCCGGATGGGGTAAGGTGGGCGGAGGATGCAGGAACGCGTGTTCCACGTAGCGAGCCCCAAGG
CCAAGCTTTACTCGGAGGCC

Product: 3-hydroxybutyryl-CoA dehydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MEHEHEHEFVLEIPEFEHLSYEVEEGIALVTLKRPEALNALSQSLLEELAEIPELVQQDPEVRAVIFTGEGKAFAAGADL
KEIAAIKDPFMGREYALFGQRVFAEIAALPVPTIAAINGYALGGGLELALACDLRVAAKTAKLGLPEVGLGLIPGFGGTQ
RLPRLIGRGRALDLIFTGRHVDAEEALFLGLVNRVAEDALEEAKKLAQKILKNAPIALALAKESVVRGEGLDLAEALEIE
ADLFGYAAATEDMKEGVRAFLEKRPPSFKGE

Sequences:

>Translated_271_residues
MEHEHEHEFVLEIPEFEHLSYEVEEGIALVTLKRPEALNALSQSLLEELAEIPELVQQDPEVRAVIFTGEGKAFAAGADL
KEIAAIKDPFMGREYALFGQRVFAEIAALPVPTIAAINGYALGGGLELALACDLRVAAKTAKLGLPEVGLGLIPGFGGTQ
RLPRLIGRGRALDLIFTGRHVDAEEALFLGLVNRVAEDALEEAKKLAQKILKNAPIALALAKESVVRGEGLDLAEALEIE
ADLFGYAAATEDMKEGVRAFLEKRPPSFKGE
>Mature_271_residues
MEHEHEHEFVLEIPEFEHLSYEVEEGIALVTLKRPEALNALSQSLLEELAEIPELVQQDPEVRAVIFTGEGKAFAAGADL
KEIAAIKDPFMGREYALFGQRVFAEIAALPVPTIAAINGYALGGGLELALACDLRVAAKTAKLGLPEVGLGLIPGFGGTQ
RLPRLIGRGRALDLIFTGRHVDAEEALFLGLVNRVAEDALEEAKKLAQKILKNAPIALALAKESVVRGEGLDLAEALEIE
ADLFGYAAATEDMKEGVRAFLEKRPPSFKGE

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=249, Percent_Identity=40.9638554216867, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI4502327, Length=268, Percent_Identity=44.0298507462687, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI150378541, Length=255, Percent_Identity=37.2549019607843, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI68989263, Length=190, Percent_Identity=42.1052631578947, Blast_Score=131, Evalue=5e-31,
Organism=Homo sapiens, GI20127408, Length=184, Percent_Identity=39.1304347826087, Blast_Score=129, Evalue=2e-30,
Organism=Homo sapiens, GI70995211, Length=270, Percent_Identity=32.962962962963, Blast_Score=128, Evalue=7e-30,
Organism=Homo sapiens, GI157694516, Length=227, Percent_Identity=31.7180616740088, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI213417737, Length=227, Percent_Identity=31.7180616740088, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI31542718, Length=253, Percent_Identity=31.6205533596838, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI157694520, Length=206, Percent_Identity=32.0388349514563, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI261878539, Length=91, Percent_Identity=56.043956043956, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI37594471, Length=148, Percent_Identity=33.7837837837838, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI37594469, Length=148, Percent_Identity=33.7837837837838, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI45643119, Length=256, Percent_Identity=24.609375, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI260275230, Length=256, Percent_Identity=24.609375, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI260274832, Length=256, Percent_Identity=24.609375, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1787659, Length=249, Percent_Identity=42.1686746987952, Blast_Score=180, Evalue=8e-47,
Organism=Escherichia coli, GI221142681, Length=251, Percent_Identity=41.0358565737052, Blast_Score=147, Evalue=5e-37,
Organism=Escherichia coli, GI1788682, Length=191, Percent_Identity=46.0732984293194, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1787660, Length=261, Percent_Identity=36.0153256704981, Blast_Score=136, Evalue=1e-33,
Organism=Escherichia coli, GI1790281, Length=197, Percent_Identity=38.5786802030457, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI1788597, Length=262, Percent_Identity=32.0610687022901, Blast_Score=117, Evalue=8e-28,
Organism=Escherichia coli, GI87082183, Length=269, Percent_Identity=30.4832713754647, Blast_Score=107, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17554946, Length=250, Percent_Identity=41.6, Blast_Score=188, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI25145438, Length=248, Percent_Identity=41.9354838709677, Blast_Score=181, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI17540714, Length=226, Percent_Identity=40.2654867256637, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17534483, Length=253, Percent_Identity=33.201581027668, Blast_Score=135, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17560910, Length=242, Percent_Identity=35.5371900826446, Blast_Score=131, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17558304, Length=215, Percent_Identity=36.7441860465116, Blast_Score=121, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17536985, Length=213, Percent_Identity=32.8638497652582, Blast_Score=118, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17508953, Length=221, Percent_Identity=36.1990950226244, Blast_Score=109, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI25144276, Length=221, Percent_Identity=36.1990950226244, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17508951, Length=221, Percent_Identity=36.1990950226244, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17540306, Length=256, Percent_Identity=30.859375, Blast_Score=100, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI25141363, Length=175, Percent_Identity=33.1428571428571, Blast_Score=98, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17549921, Length=208, Percent_Identity=30.7692307692308, Blast_Score=94, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI25144160, Length=180, Percent_Identity=33.3333333333333, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI25144157, Length=180, Percent_Identity=33.3333333333333, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320241, Length=203, Percent_Identity=28.0788177339901, Blast_Score=79, Evalue=7e-16,
Organism=Drosophila melanogaster, GI20129971, Length=266, Percent_Identity=41.7293233082707, Blast_Score=194, Evalue=6e-50,
Organism=Drosophila melanogaster, GI24653477, Length=266, Percent_Identity=41.7293233082707, Blast_Score=194, Evalue=6e-50,
Organism=Drosophila melanogaster, GI24653139, Length=254, Percent_Identity=41.7322834645669, Blast_Score=176, Evalue=1e-44,
Organism=Drosophila melanogaster, GI19922422, Length=252, Percent_Identity=29.3650793650794, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI21357171, Length=260, Percent_Identity=32.3076923076923, Blast_Score=107, Evalue=8e-24,
Organism=Drosophila melanogaster, GI19920382, Length=249, Percent_Identity=30.5220883534137, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24583077, Length=181, Percent_Identity=39.2265193370166, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24583079, Length=181, Percent_Identity=39.2265193370166, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19921000, Length=181, Percent_Identity=39.2265193370166, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24650670, Length=254, Percent_Identity=32.6771653543307, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI28571730, Length=300, Percent_Identity=27, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28571729, Length=300, Percent_Identity=27, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24654903, Length=182, Percent_Identity=25.8241758241758, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24583165, Length=209, Percent_Identity=30.622009569378, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19921018, Length=250, Percent_Identity=31.2, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 29215; Mature: 29215

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHEHEHEFVLEIPEFEHLSYEVEEGIALVTLKRPEALNALSQSLLEELAEIPELVQQDP
CCCCCCCCEEEECCCHHHCCEEHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCC
EVRAVIFTGEGKAFAAGADLKEIAAIKDPFMGREYALFGQRVFAEIAALPVPTIAAINGY
CEEEEEEECCCCEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHCCCE
ALGGGLELALACDLRVAAKTAKLGLPEVGLGLIPGFGGTQRLPRLIGRGRALDLIFTGRH
EECCCEEEEEEECHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEECCC
VDAEEALFLGLVNRVAEDALEEAKKLAQKILKNAPIALALAKESVVRGEGLDLAEALEIE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHCCCCCCHHHHHHHH
ADLFGYAAATEDMKEGVRAFLEKRPPSFKGE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MEHEHEHEFVLEIPEFEHLSYEVEEGIALVTLKRPEALNALSQSLLEELAEIPELVQQDP
CCCCCCCCEEEECCCHHHCCEEHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCC
EVRAVIFTGEGKAFAAGADLKEIAAIKDPFMGREYALFGQRVFAEIAALPVPTIAAINGY
CEEEEEEECCCCEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHCCCE
ALGGGLELALACDLRVAAKTAKLGLPEVGLGLIPGFGGTQRLPRLIGRGRALDLIFTGRH
EECCCEEEEEEECHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEECCC
VDAEEALFLGLVNRVAEDALEEAKKLAQKILKNAPIALALAKESVVRGEGLDLAEALEIE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHCCCCCCHHHHHHHH
ADLFGYAAATEDMKEGVRAFLEKRPPSFKGE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA