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The map label for this gene is rsmF [H]

Identifier: 55981356

GI number: 55981356

Start: 1323647

End: 1325017

Strand: Reverse

Name: rsmF [H]

Synonym: TTHA1387

Alternate gene names: 55981356

Gene position: 1325017-1323647 (Counterclockwise)

Preceding gene: 55981358

Following gene: 55981344

Centisome position: 71.63

GC content: 71.99

Gene sequence:

>1371_bases
GTGCTGCCCAAGGCCTTCCTTTCCCGCATGGCCGAGCTTCTGGGCGAGGAGTTTCCCGCCTTCCTCAAGGCCCTCACGGA
GGGGAAGCGCACCTACGGCCTCCGGGTGAACACCCTGAAGCTTCCCCCCGAGGCCTTTCAGAGGATCTCCCCCTGGCCCC
TACGGCCCATTCCCTGGTGCCAGGAGGGGTTTTACTACCCCGAGGAGGCCAGGCCCGGCCCCCACCCCTTCTTCTACGCC
GGGCTCTACTACATCCAGGAGCCGAGCGCCCAGGCGGTGGGGGTCCTCCTGGACCCGAAGCCCGGGGAAAGGGTCTTGGA
CCTCGCGGCGGCCCCGGGGGGCAAGACCACCCACCTCGCCGCCCGCATGGGGGGGAAGGGGCTTCTTTTGGCCAACGAGG
TGGACGGCAAACGGGTGCGGGGCCTTCTGGAGAACGTGGAGCGCTGGGGGGCCCCCCTCGCCGTGACCCAGGCCCCTCCC
AGGGCCCTGGCCGAGGCCTTCGGGACCTACTTCCACCGGGTCCTCCTGGACGCCCCCTGCTCGGGGGAGGGGATGTTCCG
CAAGGACAGGGAGGCCGCGCGCCACTGGGGGCCCTCGGCGCCCAAGAGGATGGCGGAGGTGCAGAAGGCCCTCCTGGCCC
AGGCCTCGAGGCTCCTGGGGCCTGGGGGGGTGTTGGTCTACTCCACCTGCACCTTCGCCCCCGAGGAGAACGAGGGGGTG
GTGGCCCACTTCCTCAAGGCGCACCCGGAGTTCCGCCTCGAGGACGCCCGCCTCCACCCCCTTTTCGCCCCGGGGGTGCC
GGAGTGGGGGGAGGGGAACCCGGAGCTCCTTAAGACCGCGAGGCTCTGGCCCCACCGCCTCGAGGGGGAGGGCCACTTCC
TCGCCCGCTTCCGCAAGGAGGGGGGGGCCTGGAGCACGCCGCGCCTGGAACGCCCTTCCCCCCTCTCCCAGGAGGCCCTG
CGGGCCTTTAGGGGCTTTTTGGAGGAGGCGGGGCTTACCTTGGAAGGCCCCGTCCTTGACCGGGCAGGCCACCTCTACCT
CCTCCCCGAGGGGCTTCCCACCCTCTTGGGCCTCAAGGCCCCTGCCCCGGGCCTCTACCTGGGCAAGGTGCAGAAGGGCC
GCTTCCTCCCCGCGAGGGCCCTCGCCCTCGCCTTCGGGGCCACCCTCCCTTGGCCCGAGGGGCTTCCCCGCCTCGCCCTT
ACCCCGGAAGACCCCAGGGCCCTGGCCTTCGCCACGGGGGAGGGGGTGGCCTGGGAGGGGGAGGACCACCCCCTGGCCCT
CGTGGTCCTGAAGACGGCGGCGGGGGAGTTCCCCCTGGACTTCGGCAAGGCCAAGCGCGGGGTGCTGAGGCCCGTGGGGG
TGGGGCTTTGA

Upstream 100 bases:

>100_bases
TTCCACGCGGGCGATCTCCACCAGGTCGGCCCCCAGGGCGACGATCATGGGCCCATGGTATCCCTTGAGGCCTTCGGGCC
TTCCGTGCTAGGGTACACGG

Downstream 100 bases:

>100_bases
GGCCACCCCGTGCCGGCTTGCGCCGGGGTTCCAGGTTGCGGGCGCCTAGCGGAGGAGGCCAAGCCCTTTAAGCTCCTCCC
GCCAGGCGGCGAAGGCCTCC

Product: rRNA methylase

Products: NA

Alternate protein names: 16S rRNA m5C1407 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmF [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MLPKAFLSRMAELLGEEFPAFLKALTEGKRTYGLRVNTLKLPPEAFQRISPWPLRPIPWCQEGFYYPEEARPGPHPFFYA
GLYYIQEPSAQAVGVLLDPKPGERVLDLAAAPGGKTTHLAARMGGKGLLLANEVDGKRVRGLLENVERWGAPLAVTQAPP
RALAEAFGTYFHRVLLDAPCSGEGMFRKDREAARHWGPSAPKRMAEVQKALLAQASRLLGPGGVLVYSTCTFAPEENEGV
VAHFLKAHPEFRLEDARLHPLFAPGVPEWGEGNPELLKTARLWPHRLEGEGHFLARFRKEGGAWSTPRLERPSPLSQEAL
RAFRGFLEEAGLTLEGPVLDRAGHLYLLPEGLPTLLGLKAPAPGLYLGKVQKGRFLPARALALAFGATLPWPEGLPRLAL
TPEDPRALAFATGEGVAWEGEDHPLALVVLKTAAGEFPLDFGKAKRGVLRPVGVGL

Sequences:

>Translated_456_residues
MLPKAFLSRMAELLGEEFPAFLKALTEGKRTYGLRVNTLKLPPEAFQRISPWPLRPIPWCQEGFYYPEEARPGPHPFFYA
GLYYIQEPSAQAVGVLLDPKPGERVLDLAAAPGGKTTHLAARMGGKGLLLANEVDGKRVRGLLENVERWGAPLAVTQAPP
RALAEAFGTYFHRVLLDAPCSGEGMFRKDREAARHWGPSAPKRMAEVQKALLAQASRLLGPGGVLVYSTCTFAPEENEGV
VAHFLKAHPEFRLEDARLHPLFAPGVPEWGEGNPELLKTARLWPHRLEGEGHFLARFRKEGGAWSTPRLERPSPLSQEAL
RAFRGFLEEAGLTLEGPVLDRAGHLYLLPEGLPTLLGLKAPAPGLYLGKVQKGRFLPARALALAFGATLPWPEGLPRLAL
TPEDPRALAFATGEGVAWEGEDHPLALVVLKTAAGEFPLDFGKAKRGVLRPVGVGL
>Mature_456_residues
MLPKAFLSRMAELLGEEFPAFLKALTEGKRTYGLRVNTLKLPPEAFQRISPWPLRPIPWCQEGFYYPEEARPGPHPFFYA
GLYYIQEPSAQAVGVLLDPKPGERVLDLAAAPGGKTTHLAARMGGKGLLLANEVDGKRVRGLLENVERWGAPLAVTQAPP
RALAEAFGTYFHRVLLDAPCSGEGMFRKDREAARHWGPSAPKRMAEVQKALLAQASRLLGPGGVLVYSTCTFAPEENEGV
VAHFLKAHPEFRLEDARLHPLFAPGVPEWGEGNPELLKTARLWPHRLEGEGHFLARFRKEGGAWSTPRLERPSPLSQEAL
RAFRGFLEEAGLTLEGPVLDRAGHLYLLPEGLPTLLGLKAPAPGLYLGKVQKGRFLPARALALAFGATLPWPEGLPRLAL
TPEDPRALAFATGEGVAWEGEDHPLALVVLKTAAGEFPLDFGKAKRGVLRPVGVGL

Specific function: Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=304, Percent_Identity=33.8815789473684, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI76150623, Length=304, Percent_Identity=33.8815789473684, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI270288816, Length=285, Percent_Identity=32.280701754386, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI270288818, Length=226, Percent_Identity=34.9557522123894, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI8922322, Length=226, Percent_Identity=34.9557522123894, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI23199998, Length=226, Percent_Identity=34.9557522123894, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI39995082, Length=210, Percent_Identity=29.0476190476191, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI301336155, Length=210, Percent_Identity=29.0476190476191, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI32698918, Length=188, Percent_Identity=36.7021276595745, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI11545785, Length=173, Percent_Identity=34.6820809248555, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI40316918, Length=175, Percent_Identity=31.4285714285714, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI87081985, Length=429, Percent_Identity=33.7995337995338, Blast_Score=192, Evalue=5e-50,
Organism=Escherichia coli, GI2367212, Length=242, Percent_Identity=33.4710743801653, Blast_Score=106, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17536757, Length=335, Percent_Identity=30.4477611940298, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI25143471, Length=216, Percent_Identity=33.3333333333333, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI115534021, Length=171, Percent_Identity=31.5789473684211, Blast_Score=72, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI71998419, Length=171, Percent_Identity=31.5789473684211, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324268, Length=287, Percent_Identity=33.4494773519164, Blast_Score=140, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6319447, Length=271, Percent_Identity=28.7822878228782, Blast_Score=93, Evalue=8e-20,
Organism=Drosophila melanogaster, GI22024126, Length=240, Percent_Identity=33.3333333333333, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24668781, Length=197, Percent_Identity=37.0558375634518, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI21356513, Length=236, Percent_Identity=35.5932203389831, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI21355201, Length=204, Percent_Identity=31.3725490196078, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI19920494, Length=159, Percent_Identity=35.2201257861635, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21356579, Length=189, Percent_Identity=29.6296296296296, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR011023 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu [H]

EC number: =2.1.1.178 [H]

Molecular weight: Translated: 49740; Mature: 49740

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPKAFLSRMAELLGEEFPAFLKALTEGKRTYGLRVNTLKLPPEAFQRISPWPLRPIPWC
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEECEEEEEEECCHHHHHCCCCCCCCCCCHH
QEGFYYPEEARPGPHPFFYAGLYYIQEPSAQAVGVLLDPKPGERVLDLAAAPGGKTTHLA
HCCCCCCCCCCCCCCCEEEEEEEEEECCCCCEEEEEECCCCCCHHHHEECCCCCCCEEHH
ARMGGKGLLLANEVDGKRVRGLLENVERWGAPLAVTQAPPRALAEAFGTYFHRVLLDAPC
HHCCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHCCCC
SGEGMFRKDREAARHWGPSAPKRMAEVQKALLAQASRLLGPGGVLVYSTCTFAPEENEGV
CCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCH
VAHFLKAHPEFRLEDARLHPLFAPGVPEWGEGNPELLKTARLWPHRLEGEGHFLARFRKE
HHHHHHCCCCCEECCCCCCEEECCCCCCCCCCCHHHHHHHHCCCHHCCCCCHHHHHHHHC
GGAWSTPRLERPSPLSQEALRAFRGFLEEAGLTLEGPVLDRAGHLYLLPEGLPTLLGLKA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCHHCCCCCEEECCCCCHHHHCCCC
PAPGLYLGKVQKGRFLPARALALAFGATLPWPEGLPRLALTPEDPRALAFATGEGVAWEG
CCCCEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEECCCCCCEEEEECCCCEEECC
EDHPLALVVLKTAAGEFPLDFGKAKRGVLRPVGVGL
CCCCEEEEEEEECCCCCCCCHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MLPKAFLSRMAELLGEEFPAFLKALTEGKRTYGLRVNTLKLPPEAFQRISPWPLRPIPWC
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEECEEEEEEECCHHHHHCCCCCCCCCCCHH
QEGFYYPEEARPGPHPFFYAGLYYIQEPSAQAVGVLLDPKPGERVLDLAAAPGGKTTHLA
HCCCCCCCCCCCCCCCEEEEEEEEEECCCCCEEEEEECCCCCCHHHHEECCCCCCCEEHH
ARMGGKGLLLANEVDGKRVRGLLENVERWGAPLAVTQAPPRALAEAFGTYFHRVLLDAPC
HHCCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHCCCC
SGEGMFRKDREAARHWGPSAPKRMAEVQKALLAQASRLLGPGGVLVYSTCTFAPEENEGV
CCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCH
VAHFLKAHPEFRLEDARLHPLFAPGVPEWGEGNPELLKTARLWPHRLEGEGHFLARFRKE
HHHHHHCCCCCEECCCCCCEEECCCCCCCCCCCHHHHHHHHCCCHHCCCCCHHHHHHHHC
GGAWSTPRLERPSPLSQEALRAFRGFLEEAGLTLEGPVLDRAGHLYLLPEGLPTLLGLKA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCHHCCCCCEEECCCCCHHHHCCCC
PAPGLYLGKVQKGRFLPARALALAFGATLPWPEGLPRLALTPEDPRALAFATGEGVAWEG
CCCCEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEECCCCCCEEEEECCCCEEECC
EDHPLALVVLKTAAGEFPLDFGKAKRGVLRPVGVGL
CCCCEEEEEEEECCCCCCCCHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA