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The map label for this gene is nfi

Identifier: 55981316

GI number: 55981316

Start: 1288454

End: 1289134

Strand: Direct

Name: nfi

Synonym: TTHA1347

Alternate gene names: 55981316

Gene position: 1288454-1289134 (Clockwise)

Preceding gene: 55981315

Following gene: 55981317

Centisome position: 69.66

GC content: 75.92

Gene sequence:

>681_bases
GTGCGCCCGCCCCCCTTCCCCAAGCCCGCCCACCCCGAGGAGGCCCTGGCCCTGCAGCGGGCCCTGGCGAAAAAGGTGCG
GCTTGCGGGAAGCCTAGAGGGGGTGCGGCGGATCGCCGCCCTGGACGCCTCCCACAAGCGGGGCCGGCCCCTCGTGGCCG
TGGCCCTCCTCTACGACCTGGAAAAAGGCCCCCTCCACGTGGCCACCGCCCTCCTCCCCGAGGAGGCCCTCTTCCCCTAC
GTCCCCGGCCTCCTCTCCTTCCGCGAGGCCCCGGCCTACCTGGAGGCCCTCGCCGCCCTTCCCGAGGCCCCGGAGGCCCT
CCTGGTGGACGGCCAGGGGGTGGCCCACCCCCGGGGCCTGGGGATCGCGAGCCACCTGGGGGTCCACCTGGACCTTCCCA
GCGTGGGCGTGGCCAAGCGCCTCCTCTACGGCAGGCCCGAAGCCCCCTTGCCCGAGGAAAAGGGGGCGGCGGTGCGCCTC
CTCGCCCCGGACGGCCGCCCGCTGGGCTACGTCTACCGGAGCCGCACGGGGGTGAAGCCCCTCTACGTCTCCCCCGGGCA
CCGGGTGGGCCTCGAGGAGGCCCTCCGCTTCGTGCGCCGCCTGCCCACCCGCTTCCGCCTCCCCGAGCCCCTCCGCCTCG
CCCACCTGGAGGCGGGCCGGGCCCTCAAGGCCTTAGACTAG

Upstream 100 bases:

>100_bases
GTACCGGATGGCCTGGACCGCCTACTACCTCGCCCTCCTCTACGGAGTGGACCCCGGGGACCACGGGCTTCTGGAGCGCC
TCCGGGAGGTCACCTAGGCC

Downstream 100 bases:

>100_bases
GCCCCGTGAGCGCAGCCCAGCCCAACCCCGTCTACCGGGCCTCCTGGCGCCTGGCCCGCTTCCTCCTCCGCCTTCTCTTC
CACTACCGGGTGGAAGGCGC

Product: endonuclease V

Products: NA

Alternate protein names: Deoxyinosine 3'endonuclease; Deoxyribonuclease V; DNase V

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MRPPPFPKPAHPEEALALQRALAKKVRLAGSLEGVRRIAALDASHKRGRPLVAVALLYDLEKGPLHVATALLPEEALFPY
VPGLLSFREAPAYLEALAALPEAPEALLVDGQGVAHPRGLGIASHLGVHLDLPSVGVAKRLLYGRPEAPLPEEKGAAVRL
LAPDGRPLGYVYRSRTGVKPLYVSPGHRVGLEEALRFVRRLPTRFRLPEPLRLAHLEAGRALKALD

Sequences:

>Translated_226_residues
MRPPPFPKPAHPEEALALQRALAKKVRLAGSLEGVRRIAALDASHKRGRPLVAVALLYDLEKGPLHVATALLPEEALFPY
VPGLLSFREAPAYLEALAALPEAPEALLVDGQGVAHPRGLGIASHLGVHLDLPSVGVAKRLLYGRPEAPLPEEKGAAVRL
LAPDGRPLGYVYRSRTGVKPLYVSPGHRVGLEEALRFVRRLPTRFRLPEPLRLAHLEAGRALKALD
>Mature_226_residues
MRPPPFPKPAHPEEALALQRALAKKVRLAGSLEGVRRIAALDASHKRGRPLVAVALLYDLEKGPLHVATALLPEEALFPY
VPGLLSFREAPAYLEALAALPEAPEALLVDGQGVAHPRGLGIASHLGVHLDLPSVGVAKRLLYGRPEAPLPEEKGAAVRL
LAPDGRPLGYVYRSRTGVKPLYVSPGHRVGLEEALRFVRRLPTRFRLPEPLRLAHLEAGRALKALD

Specific function: Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. Acts in DNA repair

COG id: COG1515

COG function: function code L; Deoxyinosine 3'endonuclease (endonuclease V)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the endonuclease V family

Homologues:

Organism=Homo sapiens, GI31542775, Length=206, Percent_Identity=40.7766990291262, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI257467548, Length=122, Percent_Identity=47.5409836065574, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI257467550, Length=122, Percent_Identity=47.5409836065574, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI87082357, Length=138, Percent_Identity=47.1014492753623, Blast_Score=130, Evalue=5e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NFI_THET2 (Q72IZ9)

Other databases:

- EMBL:   AE017221
- RefSeq:   YP_004951.1
- ProteinModelPortal:   Q72IZ9
- STRING:   Q72IZ9
- GeneID:   2775954
- GenomeReviews:   AE017221_GR
- eggNOG:   COG1515
- HOGENOM:   HBG716360
- OMA:   VEGHGKA
- PhylomeDB:   Q72IZ9
- ProtClustDB:   CLSK738683
- BioCyc:   TTHE262724:TT_C0982-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00801
- InterPro:   IPR007581

Pfam domain/function: PF04493 Endonuclease_5

EC number: =3.1.21.7

Molecular weight: Translated: 24431; Mature: 24431

Theoretical pI: Translated: 10.47; Mature: 10.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPPPFPKPAHPEEALALQRALAKKVRLAGSLEGVRRIAALDASHKRGRPLVAVALLYDL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHC
EKGPLHVATALLPEEALFPYVPGLLSFREAPAYLEALAALPEAPEALLVDGQGVAHPRGL
CCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCC
GIASHLGVHLDLPSVGVAKRLLYGRPEAPLPEEKGAAVRLLAPDGRPLGYVYRSRTGVKP
CHHHHCCEEECCCCHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCEECCCCCCCE
LYVSPGHRVGLEEALRFVRRLPTRFRLPEPLRLAHLEAGRALKALD
EEECCCCCCCHHHHHHHHHHCHHHCCCCCCHHHHHHHHCCHHHCCC
>Mature Secondary Structure
MRPPPFPKPAHPEEALALQRALAKKVRLAGSLEGVRRIAALDASHKRGRPLVAVALLYDL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHC
EKGPLHVATALLPEEALFPYVPGLLSFREAPAYLEALAALPEAPEALLVDGQGVAHPRGL
CCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCC
GIASHLGVHLDLPSVGVAKRLLYGRPEAPLPEEKGAAVRLLAPDGRPLGYVYRSRTGVKP
CHHHHCCEEECCCCHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCEECCCCCCCE
LYVSPGHRVGLEEALRFVRRLPTRFRLPEPLRLAHLEAGRALKALD
EEECCCCCCCHHHHHHHHHHCHHHCCCCCCHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA