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The map label for this gene is braE [H]

Identifier: 55981301

GI number: 55981301

Start: 1272404

End: 1273735

Strand: Direct

Name: braE [H]

Synonym: TTHA1332

Alternate gene names: 55981301

Gene position: 1272404-1273735 (Clockwise)

Preceding gene: 55981300

Following gene: 55981302

Centisome position: 68.79

GC content: 67.79

Gene sequence:

>1332_bases
ATGAGCCTGCTCTCCCTCGCCCTAAGCCTCGTCTTCCTCGCCCTCTCCTTCCTCGCCCCCAACACCCTCACCGCCTTCCT
GGGCCTGGGGGCCCTGGGGGTGGCCGCCTTCGCCCGGCTCGAGCCCCGGGTACGGACGTTGAACCTCGCCCTCCTCACCC
TGGCCTTCACCCTCTTCCTGCGGAACTCCGGCAACACCCTGGGGCTCATCGGCCTCGTGGGGATCCTCGTGGCCCTCACG
ACCCTCCCCCGGATCCCGCGCGGGATCCGGGTGGCCCTGGGCCTCGCCATCCTCCTCCTCTCCGTGCCCATCGCCGGGTT
CGCCAACACCTTCATCTTTGAGCTCGGCATCCAGATCGGCATCTTCGCCGCCATGTCCCTGGGCCTCAACGTGGTGGTGG
GGATGGCGGGGCTTCTGGACCTGGGTTACGCCGCCTTCTTCGCCGTGGGCGCCTACACCTGGGCCATCTTCGGCTCCCCC
CAGGCGGGGAAGTTCCTCCAGGGCAACTTTCCCCTGCCCGGCGAGTACATGTACCTCTTCATGCTCATCGCCGTGGTCAC
CACGGCCATCACCGGCCTCCTCATCGGCCTCCCCGCCCTAAGGCTCCGGGGGGACTACCTGGCCATCGTCACCCTGGGCC
TCGGCGAGGTGGTGCGCATCCTGGCCAACAACCTGGACCACCCCATCAACATCACCAACGGACCCCAGGGGATCACCCCC
GTGGGCCGTCCCCCCATTGACTGGTTCCGCAGCCTCATGGGGGCCCTGGGGGTGCGCCTGGACGAGACCACGGACTACCA
GCTCTTCTTCTACCTCCTCGTCCTGGCGATGATCGGCCTCGTGGTCCTGGCCAACGTCAACCTGGCCAACTCCCGCTTCG
GCCGCGCCTGGGTGGCCATCCGCGAGGACGAGATCGCCGCCCAGGCCATGGGCATCCCCCTCCTGCCCACCAAGCTCATC
GCCTTCATGACCGGGGCGGCCTTTTCCGGGGTGATGGGGGTGATCTACGGGGCCCAGCGCACCTTCGTCTCCCCCGAGTC
CTTCACCCTCCTGGCCTCCATCACCATCCTGGCCATGGTGATCCTGGGCGGGATGGGCTCCATCCCCGGGGCCATCCTGG
GGGCGGCCGCCCTCACCATCCTCAACCTGGACATCCTCAAGACCTTTAGCGAGTTCGTCCGCACCAGCCTCCCCTGGATC
CCGAGCCAGGTGGACCCCGCGAAGTACGAGAGGCTGGTCTTCGGGCTTATCCTCGTCTTGATGATGATCTACCGGCCCCA
AGGCCTGATCCCCGAGGCCCGCCACCGGGCCGAGCTGGAGGAGGAGGCATGA

Upstream 100 bases:

>100_bases
TCGGGACGGAGTACAAGGACGTGGTGGCCTTCCTCATCCTCATCTTCATCCTCCTCTTCCGGCCCCAAGGCCTCCTGGGC
CAGATGGTTAAGGAGAAGGT

Downstream 100 bases:

>100_bases
GGGTCCTCGAGGTCCAAGGGGTCACCAAGCGCTTCGGCGGGCTCGTGGCCGTGAACCAGGTGAGCCTGGAGGTGAACGAG
GGGGGAGATCTTCTCCGTCA

Product: branched-chain amino acid ABC transporter permease

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MSLLSLALSLVFLALSFLAPNTLTAFLGLGALGVAAFARLEPRVRTLNLALLTLAFTLFLRNSGNTLGLIGLVGILVALT
TLPRIPRGIRVALGLAILLLSVPIAGFANTFIFELGIQIGIFAAMSLGLNVVVGMAGLLDLGYAAFFAVGAYTWAIFGSP
QAGKFLQGNFPLPGEYMYLFMLIAVVTTAITGLLIGLPALRLRGDYLAIVTLGLGEVVRILANNLDHPINITNGPQGITP
VGRPPIDWFRSLMGALGVRLDETTDYQLFFYLLVLAMIGLVVLANVNLANSRFGRAWVAIREDEIAAQAMGIPLLPTKLI
AFMTGAAFSGVMGVIYGAQRTFVSPESFTLLASITILAMVILGGMGSIPGAILGAAALTILNLDILKTFSEFVRTSLPWI
PSQVDPAKYERLVFGLILVLMMIYRPQGLIPEARHRAELEEEA

Sequences:

>Translated_443_residues
MSLLSLALSLVFLALSFLAPNTLTAFLGLGALGVAAFARLEPRVRTLNLALLTLAFTLFLRNSGNTLGLIGLVGILVALT
TLPRIPRGIRVALGLAILLLSVPIAGFANTFIFELGIQIGIFAAMSLGLNVVVGMAGLLDLGYAAFFAVGAYTWAIFGSP
QAGKFLQGNFPLPGEYMYLFMLIAVVTTAITGLLIGLPALRLRGDYLAIVTLGLGEVVRILANNLDHPINITNGPQGITP
VGRPPIDWFRSLMGALGVRLDETTDYQLFFYLLVLAMIGLVVLANVNLANSRFGRAWVAIREDEIAAQAMGIPLLPTKLI
AFMTGAAFSGVMGVIYGAQRTFVSPESFTLLASITILAMVILGGMGSIPGAILGAAALTILNLDILKTFSEFVRTSLPWI
PSQVDPAKYERLVFGLILVLMMIYRPQGLIPEARHRAELEEEA
>Mature_442_residues
SLLSLALSLVFLALSFLAPNTLTAFLGLGALGVAAFARLEPRVRTLNLALLTLAFTLFLRNSGNTLGLIGLVGILVALTT
LPRIPRGIRVALGLAILLLSVPIAGFANTFIFELGIQIGIFAAMSLGLNVVVGMAGLLDLGYAAFFAVGAYTWAIFGSPQ
AGKFLQGNFPLPGEYMYLFMLIAVVTTAITGLLIGLPALRLRGDYLAIVTLGLGEVVRILANNLDHPINITNGPQGITPV
GRPPIDWFRSLMGALGVRLDETTDYQLFFYLLVLAMIGLVVLANVNLANSRFGRAWVAIREDEIAAQAMGIPLLPTKLIA
FMTGAAFSGVMGVIYGAQRTFVSPESFTLLASITILAMVILGGMGSIPGAILGAAALTILNLDILKTFSEFVRTSLPWIP
SQVDPAKYERLVFGLILVLMMIYRPQGLIPEARHRAELEEEA

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=338, Percent_Identity=39.6449704142012, Blast_Score=186, Evalue=2e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 47272; Mature: 47141

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLLSLALSLVFLALSFLAPNTLTAFLGLGALGVAAFARLEPRVRTLNLALLTLAFTLFL
CHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
RNSGNTLGLIGLVGILVALTTLPRIPRGIRVALGLAILLLSVPIAGFANTFIFELGIQIG
HCCCCEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
IFAAMSLGLNVVVGMAGLLDLGYAAFFAVGAYTWAIFGSPQAGKFLQGNFPLPGEYMYLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCHHHHHHH
MLIAVVTTAITGLLIGLPALRLRGDYLAIVTLGLGEVVRILANNLDHPINITNGPQGITP
HHHHHHHHHHHHHHHHCHHHHCCCCEEEEEHHHHHHHHHHHHHCCCCCEECCCCCCCCCC
VGRPPIDWFRSLMGALGVRLDETTDYQLFFYLLVLAMIGLVVLANVNLANSRFGRAWVAI
CCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
REDEIAAQAMGIPLLPTKLIAFMTGAAFSGVMGVIYGAQRTFVSPESFTLLASITILAMV
EHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCHHHHHHHHHHHHHH
ILGGMGSIPGAILGAAALTILNLDILKTFSEFVRTSLPWIPSQVDPAKYERLVFGLILVL
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
MMIYRPQGLIPEARHRAELEEEA
HHHHCCCCCCHHHHHHHHCCCCC
>Mature Secondary Structure 
SLLSLALSLVFLALSFLAPNTLTAFLGLGALGVAAFARLEPRVRTLNLALLTLAFTLFL
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
RNSGNTLGLIGLVGILVALTTLPRIPRGIRVALGLAILLLSVPIAGFANTFIFELGIQIG
HCCCCEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
IFAAMSLGLNVVVGMAGLLDLGYAAFFAVGAYTWAIFGSPQAGKFLQGNFPLPGEYMYLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCHHHHHHH
MLIAVVTTAITGLLIGLPALRLRGDYLAIVTLGLGEVVRILANNLDHPINITNGPQGITP
HHHHHHHHHHHHHHHHCHHHHCCCCEEEEEHHHHHHHHHHHHHCCCCCEECCCCCCCCCC
VGRPPIDWFRSLMGALGVRLDETTDYQLFFYLLVLAMIGLVVLANVNLANSRFGRAWVAI
CCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
REDEIAAQAMGIPLLPTKLIAFMTGAAFSGVMGVIYGAQRTFVSPESFTLLASITILAMV
EHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCHHHHHHHHHHHHHH
ILGGMGSIPGAILGAAALTILNLDILKTFSEFVRTSLPWIPSQVDPAKYERLVFGLILVL
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
MMIYRPQGLIPEARHRAELEEEA
HHHHCCCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]