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The map label for this gene is rimO [H]

Identifier: 55981144

GI number: 55981144

Start: 1121510

End: 1122814

Strand: Reverse

Name: rimO [H]

Synonym: TTHA1175

Alternate gene names: 55981144

Gene position: 1122814-1121510 (Counterclockwise)

Preceding gene: 55981145

Following gene: 55981143

Centisome position: 60.7

GC content: 69.27

Gene sequence:

>1305_bases
ATGGCGAAGATCGGTTTTGTCAGCTTGGGCTGCCCCAAAGCCCTGGTGGACTCGGAGCAGATCCTTTCCCGGCTCAAGGC
CCTGGGGTACGAGACGAGCCCCAGCTACGAGGAGGCGGAGCTCGTCATCGTCAACACCTGCGGCTTCATCAACGAGGCGG
TGGAGGAGAGCCTCGAGGTCATCGGCGAGGCCCTCAAGGAAAACGGCAAGGTGGTGGTCACGGGCTGCCTGGGGGCGCGC
CCCGAGAAGATCCGGGAGCGCCACCCCCAGGTGCTCGCCATCACGGGCCCGGGGGAGGTGGAAAGGGTTCTGGAGGCGGT
GCAGGTGGTGCTTCCCGCGCCCCGGGACCCCTTTTTGGACCTCATCCCTCCCCAGGTCAAGCTCACCCCGAGGCACTACG
CCTACGTGAAGCTCTCCGAGGGGTGCGACCACCGGTGCAGCTTCTGCATCATCCCTAAGCTGCGGGGAAGGCTCCGCTCC
CGGGACGCCGCCGACGTGCTCGCCGAGGCGGCGAGGCTCGTGGCCACGGGGACGAAGGAGCTCCTCCTCGTGGCCCAGGA
CCTCTCGGCCTACGGGGTGGACCTGGGGCACCGGGAGAGCCTCTGGGGGGACCGGCCCGTGCGGGCGGAGCTTAAGGACC
TCCTCGCCCACATGGCCGAGCTCGGCGCCTGGATCCGCCTCCACTACGTCTACCCCTACCCCCATGTCAAGGACCTCCTC
CCCCTCATGGCCGAGGGGAAGGTCCTGCCCTACCTGGACGTTCCCCTCCAGCACGCCTCCCCCCGGATCCTCCGCCTCAT
GCGCCGCCCCGGGGGGTACGAGAGCCACCTCAAGGCCCTCAAGGCCTGGCGGGAGGTGGTGCCCGAGCTCGCCCTCCGCT
CCACCTTCATCGTGGGCTTCCCCGGGGAGACGGAGGAGGACTTCCAGATCCTCCTGGACTTCCTGGAGGAGGCGGAGCTG
GACCGGGTGGGGGTCTTCGCCTACTCCCCGGTGGAGGGGGCGGAGGCGAACCGCCTTCCGGACCCCGTGCCCGAGGAGGT
GAAGGAGGAGCGCAAGGCGAGGCTTTTGGAGCTGCAGGCCCGGGTGAGCCTCAGGAAGAACCAGCGTTTCGTGGGCAAGA
CCCTGGAGGTCCTGGTGGACGAGCTGCCCGAGCCCGGCCTCGCCGTGGGCCGGACCTACCGCGACTCCCCCGGCATAGAC
GGGGTGGTCTACGTGGAGACGGACGGCACCGTGCGGGTAGGGGAGAGGATCCCCGTGCGCATCCTTCGGGCGGACACCTA
CGACCTCCACGGGGTCCAGGCGTAG

Upstream 100 bases:

>100_bases
GGGAAGGACCTCGGGCCCATGGGCGAGCCCGGCATCCCCGTCACCCGGCGCTACCCCTGATATACTTGGGGTTTGTTGGG
GCCGAGCCCCCGGGAGGCGT

Downstream 100 bases:

>100_bases
GATAGGGGCGGCCATGTACATCGTCATCGCTGGGGGCGGGGAGATGGGCGGGGAGCTCGCCCGCGCCCTGGAGAAGACCC
ACGACGTGGTGGTCATTGAC

Product: ribosomal protein S12 methylthiotransferase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO [H]

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MAKIGFVSLGCPKALVDSEQILSRLKALGYETSPSYEEAELVIVNTCGFINEAVEESLEVIGEALKENGKVVVTGCLGAR
PEKIRERHPQVLAITGPGEVERVLEAVQVVLPAPRDPFLDLIPPQVKLTPRHYAYVKLSEGCDHRCSFCIIPKLRGRLRS
RDAADVLAEAARLVATGTKELLLVAQDLSAYGVDLGHRESLWGDRPVRAELKDLLAHMAELGAWIRLHYVYPYPHVKDLL
PLMAEGKVLPYLDVPLQHASPRILRLMRRPGGYESHLKALKAWREVVPELALRSTFIVGFPGETEEDFQILLDFLEEAEL
DRVGVFAYSPVEGAEANRLPDPVPEEVKEERKARLLELQARVSLRKNQRFVGKTLEVLVDELPEPGLAVGRTYRDSPGID
GVVYVETDGTVRVGERIPVRILRADTYDLHGVQA

Sequences:

>Translated_434_residues
MAKIGFVSLGCPKALVDSEQILSRLKALGYETSPSYEEAELVIVNTCGFINEAVEESLEVIGEALKENGKVVVTGCLGAR
PEKIRERHPQVLAITGPGEVERVLEAVQVVLPAPRDPFLDLIPPQVKLTPRHYAYVKLSEGCDHRCSFCIIPKLRGRLRS
RDAADVLAEAARLVATGTKELLLVAQDLSAYGVDLGHRESLWGDRPVRAELKDLLAHMAELGAWIRLHYVYPYPHVKDLL
PLMAEGKVLPYLDVPLQHASPRILRLMRRPGGYESHLKALKAWREVVPELALRSTFIVGFPGETEEDFQILLDFLEEAEL
DRVGVFAYSPVEGAEANRLPDPVPEEVKEERKARLLELQARVSLRKNQRFVGKTLEVLVDELPEPGLAVGRTYRDSPGID
GVVYVETDGTVRVGERIPVRILRADTYDLHGVQA
>Mature_433_residues
AKIGFVSLGCPKALVDSEQILSRLKALGYETSPSYEEAELVIVNTCGFINEAVEESLEVIGEALKENGKVVVTGCLGARP
EKIRERHPQVLAITGPGEVERVLEAVQVVLPAPRDPFLDLIPPQVKLTPRHYAYVKLSEGCDHRCSFCIIPKLRGRLRSR
DAADVLAEAARLVATGTKELLLVAQDLSAYGVDLGHRESLWGDRPVRAELKDLLAHMAELGAWIRLHYVYPYPHVKDLLP
LMAEGKVLPYLDVPLQHASPRILRLMRRPGGYESHLKALKAWREVVPELALRSTFIVGFPGETEEDFQILLDFLEEAELD
RVGVFAYSPVEGAEANRLPDPVPEEVKEERKARLLELQARVSLRKNQRFVGKTLEVLVDELPEPGLAVGRTYRDSPGIDG
VVYVETDGTVRVGERIPVRILRADTYDLHGVQA

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12 [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=424, Percent_Identity=28.5377358490566, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI28872782, Length=424, Percent_Identity=28.5377358490566, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI93277076, Length=403, Percent_Identity=26.7990074441687, Blast_Score=137, Evalue=2e-32,
Organism=Escherichia coli, GI1787057, Length=431, Percent_Identity=61.7169373549884, Blast_Score=552, Evalue=1e-158,
Organism=Escherichia coli, GI1786882, Length=397, Percent_Identity=30.2267002518892, Blast_Score=163, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17553146, Length=447, Percent_Identity=26.1744966442953, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI71996771, Length=306, Percent_Identity=26.797385620915, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21356207, Length=478, Percent_Identity=26.9874476987448, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI19922432, Length=450, Percent_Identity=25.3333333333333, Blast_Score=118, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 48283; Mature: 48152

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS50926 TRAM ; PS00430 TONB_DEPENDENT_REC_1 ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKIGFVSLGCPKALVDSEQILSRLKALGYETSPSYEEAELVIVNTCGFINEAVEESLEV
CCCEEEEECCCCHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHH
IGEALKENGKVVVTGCLGARPEKIRERHPQVLAITGPGEVERVLEAVQVVLPAPRDPFLD
HHHHHHCCCCEEEEECCCCCHHHHHHHCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCC
LIPPQVKLTPRHYAYVKLSEGCDHRCSFCIIPKLRGRLRSRDAADVLAEAARLVATGTKE
CCCCCEEECCCEEEEEEECCCCCCCCCEEEEHHHHHHHCCCCHHHHHHHHHHHHHCCCHH
LLLVAQDLSAYGVDLGHRESLWGDRPVRAELKDLLAHMAELGAWIRLHYVYPYPHVKDLL
HEEEEHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHH
PLMAEGKVLPYLDVPLQHASPRILRLMRRPGGYESHLKALKAWREVVPELALRSTFIVGF
HHHHCCCCCEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEEC
PGETEEDFQILLDFLEEAELDRVGVFAYSPVEGAEANRLPDPVPEEVKEERKARLLELQA
CCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
RVSLRKNQRFVGKTLEVLVDELPEPGLAVGRTYRDSPGIDGVVYVETDGTVRVGERIPVR
HHHHHHCHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCEEEEEECCCCEEECCCCCEE
ILRADTYDLHGVQA
EEECCCCCCCCCCC
>Mature Secondary Structure 
AKIGFVSLGCPKALVDSEQILSRLKALGYETSPSYEEAELVIVNTCGFINEAVEESLEV
CCEEEEECCCCHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHH
IGEALKENGKVVVTGCLGARPEKIRERHPQVLAITGPGEVERVLEAVQVVLPAPRDPFLD
HHHHHHCCCCEEEEECCCCCHHHHHHHCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCC
LIPPQVKLTPRHYAYVKLSEGCDHRCSFCIIPKLRGRLRSRDAADVLAEAARLVATGTKE
CCCCCEEECCCEEEEEEECCCCCCCCCEEEEHHHHHHHCCCCHHHHHHHHHHHHHCCCHH
LLLVAQDLSAYGVDLGHRESLWGDRPVRAELKDLLAHMAELGAWIRLHYVYPYPHVKDLL
HEEEEHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHH
PLMAEGKVLPYLDVPLQHASPRILRLMRRPGGYESHLKALKAWREVVPELALRSTFIVGF
HHHHCCCCCEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEEC
PGETEEDFQILLDFLEEAELDRVGVFAYSPVEGAEANRLPDPVPEEVKEERKARLLELQA
CCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
RVSLRKNQRFVGKTLEVLVDELPEPGLAVGRTYRDSPGIDGVVYVETDGTVRVGERIPVR
HHHHHHCHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCEEEEEECCCCEEECCCCCEE
ILRADTYDLHGVQA
EEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA