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The map label for this gene is murD

Identifier: 55981051

GI number: 55981051

Start: 1028819

End: 1030072

Strand: Direct

Name: murD

Synonym: TTHA1082

Alternate gene names: 55981051

Gene position: 1028819-1030072 (Clockwise)

Preceding gene: 55981050

Following gene: 55981052

Centisome position: 55.62

GC content: 72.89

Gene sequence:

>1254_bases
ATGAGGCTCGTCTACGGCCTGGGACGGAGCGGCCTCGGCGTCCTCCGCTTCCTGAGGAAACGGGGGCTTCCCGCCCGCTT
CTACGACGACCGCCCCCGGGAAGAGGAGGTGAGGGAGGCTTTGGCCCTGGGCTTCCAGCCGGACTGGAACCTGGAGGAAG
CCTACGAGGAGGTGGTGGCCGCCCCCGGGGTGCCCCTGGACCACCCCCACCTGAAAAGGCTCGCCGCCCGGGGCGCCAAG
GTCCTCGGGGAGGCGGAGCTCGCCTACCGCCTCTCCCCCACCCCCATCGTGGGGATCACGGGAACCGCGGGCAAGACCAG
CACCACCCTCTTCACCGCCCACCTCCTCCGCGCCCACGGGCTTAGGGCCCGGGAGGGGGGGAACGTGGACCCTCCACTCG
TGAGCGTGGTGGACGAGGCGGAGGTGGCCGTGGCGGAGCTCTCCAGCTTCCAGCTGGAAAGGGTCCACGCCTTCCGCCCC
AGGGTGGCCGTCCTCCTGAACCTCGGGGTGGACCACCTGGACCGGCACGGCACCGTGGAGGCCTACCACGCCGCCAAGCT
CAACCTCCTCAAAAACCTCACCCCCGAGGACGCCCTCGTCTACAACCGCCTGGACCCCAAGGTGCGCCGGGCGGCGGAGG
CGAGCCCCGCCCGCCTCTACCCCTTCACCCCGGGGTCCACGCCCAGGGAGACCAACCTCAGGGCCGCCTTGGAGGCCACC
CGGGCCTACCTGGACCTCCTGGGGAGGCCTCTGGACCCCGGGGCCGTGGCCGAGGCCCTAAGGACCCTCCCCGAGGCCCC
CCACCGCTTCCAGGCCTTCGCCCGCAAGGGGGGGGTGGTCTTCATTGACGACTCCATCGCCACGAGGACCGAGGCGGTGC
GGGCCGCCCTCGAGGCCGCCCCCGCCCCCATCGCCTGGATCCTGGGCGGGGAGGACAAAGGGGCCGACCTCGCCCCCCTC
CTCCCCCTCCTCGGCCGGGTCCGGGTGGCCCTGGCCCTCGGCCGGGACGGCCCCCGCTTCGCCCGGGCCCTGGAGGGGAG
GACCGAGGTGGTGGTCATCGCGGAGAAGGACGGCCGCACCGCCATGCGCAAGGCGGTGGAGGAGGCCCTCTCCCGGCTGG
AGCAGGGGAGCGTCCTCCTCGCGCCCCTGGCGGCGAGCTTTGACCAGTTCCGCGACTACAAGGACCGCGCCCAGGCCTTC
CGGGAAGCGGTCTTCGCCTTGGGAGGTGAGCCGTGGACCCCGTCCTCCTCCTAA

Upstream 100 bases:

>100_bases
ACCACCACTTTGAGCTCCTCGGCTGGGACGAGGCCAAGATCGTCTTCCGCTTCGCCGTGGTCACCGCCTTGGCCGTGGCC
CTGGCCTTCGGAGGTGGGGG

Downstream 100 bases:

>100_bases
GCGCCCTCCTCCTCCTCGCCTTCGGCCTCATCGGCGTGGGCACGGCGGAGCCCGGCCTCCTCCAGGATCACCTCCTCCGG
GTGGGGCCGCCCTCCTGGCC

Product: UDP-N-acetylmuramoylalanine--D-glutamiate ligase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MRLVYGLGRSGLGVLRFLRKRGLPARFYDDRPREEEVREALALGFQPDWNLEEAYEEVVAAPGVPLDHPHLKRLAARGAK
VLGEAELAYRLSPTPIVGITGTAGKTSTTLFTAHLLRAHGLRAREGGNVDPPLVSVVDEAEVAVAELSSFQLERVHAFRP
RVAVLLNLGVDHLDRHGTVEAYHAAKLNLLKNLTPEDALVYNRLDPKVRRAAEASPARLYPFTPGSTPRETNLRAALEAT
RAYLDLLGRPLDPGAVAEALRTLPEAPHRFQAFARKGGVVFIDDSIATRTEAVRAALEAAPAPIAWILGGEDKGADLAPL
LPLLGRVRVALALGRDGPRFARALEGRTEVVVIAEKDGRTAMRKAVEEALSRLEQGSVLLAPLAASFDQFRDYKDRAQAF
REAVFALGGEPWTPSSS

Sequences:

>Translated_417_residues
MRLVYGLGRSGLGVLRFLRKRGLPARFYDDRPREEEVREALALGFQPDWNLEEAYEEVVAAPGVPLDHPHLKRLAARGAK
VLGEAELAYRLSPTPIVGITGTAGKTSTTLFTAHLLRAHGLRAREGGNVDPPLVSVVDEAEVAVAELSSFQLERVHAFRP
RVAVLLNLGVDHLDRHGTVEAYHAAKLNLLKNLTPEDALVYNRLDPKVRRAAEASPARLYPFTPGSTPRETNLRAALEAT
RAYLDLLGRPLDPGAVAEALRTLPEAPHRFQAFARKGGVVFIDDSIATRTEAVRAALEAAPAPIAWILGGEDKGADLAPL
LPLLGRVRVALALGRDGPRFARALEGRTEVVVIAEKDGRTAMRKAVEEALSRLEQGSVLLAPLAASFDQFRDYKDRAQAF
REAVFALGGEPWTPSSS
>Mature_417_residues
MRLVYGLGRSGLGVLRFLRKRGLPARFYDDRPREEEVREALALGFQPDWNLEEAYEEVVAAPGVPLDHPHLKRLAARGAK
VLGEAELAYRLSPTPIVGITGTAGKTSTTLFTAHLLRAHGLRAREGGNVDPPLVSVVDEAEVAVAELSSFQLERVHAFRP
RVAVLLNLGVDHLDRHGTVEAYHAAKLNLLKNLTPEDALVYNRLDPKVRRAAEASPARLYPFTPGSTPRETNLRAALEAT
RAYLDLLGRPLDPGAVAEALRTLPEAPHRFQAFARKGGVVFIDDSIATRTEAVRAALEAAPAPIAWILGGEDKGADLAPL
LPLLGRVRVALALGRDGPRFARALEGRTEVVVIAEKDGRTAMRKAVEEALSRLEQGSVLLAPLAASFDQFRDYKDRAQAF
REAVFALGGEPWTPSSS

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=436, Percent_Identity=32.1100917431193, Blast_Score=157, Evalue=8e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_THET2 (Q72JQ1)

Other databases:

- EMBL:   AE017221
- RefSeq:   YP_004692.1
- ProteinModelPortal:   Q72JQ1
- STRING:   Q72JQ1
- GeneID:   2776329
- GenomeReviews:   AE017221_GR
- eggNOG:   COG0771
- HOGENOM:   HBG750024
- OMA:   VKYYNDS
- PhylomeDB:   Q72JQ1
- ProtClustDB:   CLSK445620
- BioCyc:   TTHE262724:TT_C0717-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 45323; Mature: 45323

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLVYGLGRSGLGVLRFLRKRGLPARFYDDRPREEEVREALALGFQPDWNLEEAYEEVVA
CEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHH
APGVPLDHPHLKRLAARGAKVLGEAELAYRLSPTPIVGITGTAGKTSTTLFTAHLLRAHG
CCCCCCCCHHHHHHHHHHHHHHCCHHHEEEECCCCEEEEECCCCCCHHHHHHHHHHHHCC
LRAREGGNVDPPLVSVVDEAEVAVAELSSFQLERVHAFRPRVAVLLNLGVDHLDRHGTVE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHCCCCCCH
AYHAAKLNLLKNLTPEDALVYNRLDPKVRRAAEASPARLYPFTPGSTPRETNLRAALEAT
HHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHH
RAYLDLLGRPLDPGAVAEALRTLPEAPHRFQAFARKGGVVFIDDSIATRTEAVRAALEAA
HHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHC
PAPIAWILGGEDKGADLAPLLPLLGRVRVALALGRDGPRFARALEGRTEVVVIAEKDGRT
CCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEECCCCHH
AMRKAVEEALSRLEQGSVLLAPLAASFDQFRDYKDRAQAFREAVFALGGEPWTPSSS
HHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MRLVYGLGRSGLGVLRFLRKRGLPARFYDDRPREEEVREALALGFQPDWNLEEAYEEVVA
CEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHH
APGVPLDHPHLKRLAARGAKVLGEAELAYRLSPTPIVGITGTAGKTSTTLFTAHLLRAHG
CCCCCCCCHHHHHHHHHHHHHHCCHHHEEEECCCCEEEEECCCCCCHHHHHHHHHHHHCC
LRAREGGNVDPPLVSVVDEAEVAVAELSSFQLERVHAFRPRVAVLLNLGVDHLDRHGTVE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHCCCCCCH
AYHAAKLNLLKNLTPEDALVYNRLDPKVRRAAEASPARLYPFTPGSTPRETNLRAALEAT
HHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHH
RAYLDLLGRPLDPGAVAEALRTLPEAPHRFQAFARKGGVVFIDDSIATRTEAVRAALEAA
HHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHC
PAPIAWILGGEDKGADLAPLLPLLGRVRVALALGRDGPRFARALEGRTEVVVIAEKDGRT
CCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEECCCCHH
AMRKAVEEALSRLEQGSVLLAPLAASFDQFRDYKDRAQAFREAVFALGGEPWTPSSS
HHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA