Click here to switch to the map view.

The map label for this gene is rpfG [H]

Identifier: 55980964

GI number: 55980964

Start: 939555

End: 940523

Strand: Direct

Name: rpfG [H]

Synonym: TTHA0995

Alternate gene names: 55980964

Gene position: 939555-940523 (Clockwise)

Preceding gene: 55980963

Following gene: 55980966

Centisome position: 50.79

GC content: 71.83

Gene sequence:

>969_bases
ATGAACCCCACGGTGCTCGTGGCCGAGGACGACCCCGTCCAGCGCCTTCTCCTCCGCCGGGCCTTGGAGCCTCTCGGCGT
GGAGGTGCGCCTCGCCCGCCACGGCAGGGAGGCCTTGGACCTGGCCCTGGAGGCCCCGCCCCATCTGGTCCTCACCGACC
TCCACATGCCCCACATGGACGGGCTGGAGCTCACGCGAAGGCTCAAGGCCCTGGACCCCTTGCTCCCCGTGCTCCTCTTC
ACGGCGGACCGGGACCACGAGACCCGGCTTAGGGGCCTGGAGGCGGGGGCTGACGACTTCCTGAACCGCCCCTTTGACCT
CACCGAGCTCCGGCTCCGGGTGAAGGGGCATCTGGAGCGGAGGCGGCTTCAGGAGAAGCTGGAGGACCTGGAGCGGGCCC
TCCTCGCCCTGGTGCGGGCGGTGGAGGCCAAGGACCCCTACACCGCAGGCCACGCGGAGCGGGTCGCCCGCTACGCCCTC
CTGGGGGCGCGGGAGCTTGGGGCCGGTGAGGAGGAGCTCCGGGACCTCCACATGGGGGCGCTCCTCCACGACGTGGGCAA
GATCGGCCTGCCCGACCGCATCCTTCGGGGAGAGGCCCCGCTTTCGGCAGCGGACTGGCGGCTCATCCAAGCCCACCCCG
TCAAGGGGGACGAGATCCTCACGCCCCTGAAGGCCCACCCCCGCCTCCGTCCCTATGTGCGCTGGCACCACGAGAAGCTG
GACGGCTCCGGCTACCCCGACGGCCTCACGGAGATCCCCCTCCTGGTCCAGGTCCTGAGCGCCGCCGACATGTACGACGC
CCTCACCAGCCGCAGGACCTACCGGGAGGCCGTAAAGCCCGAGGAAGCCCTGGAGGCCCTGGAGCGGGAGGCCCGGGAGG
GGAGGCTCGCCCTCGAGGCGGTGCGGGCCTTGCGGTCGGGCCTCCGGCGCGGGGCCTCAGAGGCCAAAGGACACGTACTT
CACCTCTAG

Upstream 100 bases:

>100_bases
TGGTCCGCCTTTACCTCCTGAAGCTGGAGCGCTTGGGCGCCCTGCGGCGGGAGGCCCAAAAAGGCGCGGCCCCGCTACTT
TTCGGCAAGGGAGGCGCCGC

Downstream 100 bases:

>100_bases
GTACTCCAAAAGGCCCCACTTCCCCCCTTCCCGGCCGAGCCCCGAGCGCTTCACGCCGCCAAAGGGGGCCTGCGGGGTGG
AGGGCACGCCGTCGTTCACC

Product: response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MNPTVLVAEDDPVQRLLLRRALEPLGVEVRLARHGREALDLALEAPPHLVLTDLHMPHMDGLELTRRLKALDPLLPVLLF
TADRDHETRLRGLEAGADDFLNRPFDLTELRLRVKGHLERRRLQEKLEDLERALLALVRAVEAKDPYTAGHAERVARYAL
LGARELGAGEEELRDLHMGALLHDVGKIGLPDRILRGEAPLSAADWRLIQAHPVKGDEILTPLKAHPRLRPYVRWHHEKL
DGSGYPDGLTEIPLLVQVLSAADMYDALTSRRTYREAVKPEEALEALEREAREGRLALEAVRALRSGLRRGASEAKGHVL
HL

Sequences:

>Translated_322_residues
MNPTVLVAEDDPVQRLLLRRALEPLGVEVRLARHGREALDLALEAPPHLVLTDLHMPHMDGLELTRRLKALDPLLPVLLF
TADRDHETRLRGLEAGADDFLNRPFDLTELRLRVKGHLERRRLQEKLEDLERALLALVRAVEAKDPYTAGHAERVARYAL
LGARELGAGEEELRDLHMGALLHDVGKIGLPDRILRGEAPLSAADWRLIQAHPVKGDEILTPLKAHPRLRPYVRWHHEKL
DGSGYPDGLTEIPLLVQVLSAADMYDALTSRRTYREAVKPEEALEALEREAREGRLALEAVRALRSGLRRGASEAKGHVL
HL
>Mature_322_residues
MNPTVLVAEDDPVQRLLLRRALEPLGVEVRLARHGREALDLALEAPPHLVLTDLHMPHMDGLELTRRLKALDPLLPVLLF
TADRDHETRLRGLEAGADDFLNRPFDLTELRLRVKGHLERRRLQEKLEDLERALLALVRAVEAKDPYTAGHAERVARYAL
LGARELGAGEEELRDLHMGALLHDVGKIGLPDRILRGEAPLSAADWRLIQAHPVKGDEILTPLKAHPRLRPYVRWHHEKL
DGSGYPDGLTEIPLLVQVLSAADMYDALTSRRTYREAVKPEEALEALEREAREGRLALEAVRALRSGLRRGASEAKGHVL
HL

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=172, Percent_Identity=31.9767441860465, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1790437, Length=110, Percent_Identity=39.0909090909091, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI145693157, Length=118, Percent_Identity=36.4406779661017, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1790299, Length=105, Percent_Identity=37.1428571428571, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788394, Length=127, Percent_Identity=35.4330708661417, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789809, Length=120, Percent_Identity=35.8333333333333, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786784, Length=116, Percent_Identity=35.3448275862069, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1790860, Length=117, Percent_Identity=38.4615384615385, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787375, Length=117, Percent_Identity=34.1880341880342, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI87082012, Length=116, Percent_Identity=31.8965517241379, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1786911, Length=117, Percent_Identity=35.8974358974359, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790863, Length=119, Percent_Identity=35.2941176470588, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786599, Length=118, Percent_Identity=33.8983050847458, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1788713, Length=108, Percent_Identity=38.8888888888889, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787229, Length=160, Percent_Identity=28.75, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1789402, Length=124, Percent_Identity=32.258064516129, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 36203; Mature: 36203

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00732 RIBOSOMAL_S16

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPTVLVAEDDPVQRLLLRRALEPLGVEVRLARHGREALDLALEAPPHLVLTDLHMPHMD
CCCCEEEECCCHHHHHHHHHHHCCCCCEEHHHHCCHHHHHHHHCCCCCEEEEECCCCCCC
GLELTRRLKALDPLLPVLLFTADRDHETRLRGLEAGADDFLNRPFDLTELRLRVKGHLER
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHH
RRLQEKLEDLERALLALVRAVEAKDPYTAGHAERVARYALLGARELGAGEEELRDLHMGA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LLHDVGKIGLPDRILRGEAPLSAADWRLIQAHPVKGDEILTPLKAHPRLRPYVRWHHEKL
HHHHHHCCCCCHHHHCCCCCCCHHCCEEEEECCCCCCHHCCCHHCCCCCCHHHHHHHHHC
DGSGYPDGLTEIPLLVQVLSAADMYDALTSRRTYREAVKPEEALEALEREAREGRLALEA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
VRALRSGLRRGASEAKGHVLHL
HHHHHHHHHCCHHHHCCCCCCC
>Mature Secondary Structure
MNPTVLVAEDDPVQRLLLRRALEPLGVEVRLARHGREALDLALEAPPHLVLTDLHMPHMD
CCCCEEEECCCHHHHHHHHHHHCCCCCEEHHHHCCHHHHHHHHCCCCCEEEEECCCCCCC
GLELTRRLKALDPLLPVLLFTADRDHETRLRGLEAGADDFLNRPFDLTELRLRVKGHLER
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHH
RRLQEKLEDLERALLALVRAVEAKDPYTAGHAERVARYALLGARELGAGEEELRDLHMGA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LLHDVGKIGLPDRILRGEAPLSAADWRLIQAHPVKGDEILTPLKAHPRLRPYVRWHHEKL
HHHHHHCCCCCHHHHCCCCCCCHHCCEEEEECCCCCCHHCCCHHCCCCCCHHHHHHHHHC
DGSGYPDGLTEIPLLVQVLSAADMYDALTSRRTYREAVKPEEALEALEREAREGRLALEA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
VRALRSGLRRGASEAKGHVLHL
HHHHHHHHHCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]