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The map label for this gene is nusA

Identifier: 55980670

GI number: 55980670

Start: 664317

End: 665480

Strand: Reverse

Name: nusA

Synonym: TTHA0701

Alternate gene names: 55980670

Gene position: 665480-664317 (Counterclockwise)

Preceding gene: 55980671

Following gene: 55980669

Centisome position: 35.98

GC content: 66.32

Gene sequence:

>1164_bases
ATGAACAGGGAGTTCGTGGAAGCCATGCAGCACCTGGCCCTGGAGCGCGGGGTCAGCGTGGAGGAGATCCTGGAGGCCTT
CAAGGAGGCCCTGCGCAAGGCCTACATCAAGCGGCAGAAGGGCTACAAGAAGGAGGAGATTGACCAGGGGAAGGGCCCCG
AGGTGGACGTCTACATTGACCCGAACTCGGGCCGCATTGAGATGGTGGAGGTCCGGCGGGTGGTGGAGAAGGTGGAGGAC
CCCGACAAGGAGATCGCCCTCGCCGAGGCCCTCCAGTACGACCCCGAGGTCCAGGTGGGGGACGAGATGGAGTTCCCCAT
CAACCCGGAGGAGCTCTCCCGCATGGCCATCCAGGACCTGCGCCAGATCCTGACCCAGCGCCTCAAGGAGTCGGAGCGCA
ACCGGATCTACAACGAGTACAAGGACAAGGAGGGGCAGGTCCTCACCGGGGTGGTCACCCGGGTGGACAACCGGGGCAAC
GTCTTCGTGGAGCTGGGCCGGGGCGAGGCCTACCTCCCCCGGAGCGAGCAGATCCCCACGGAGAAGTACCACCCCGGCCA
GCGGATCAAGGTCTACCTGAAGAAGGTGGACAAGTCCGCCAAGGGCCCCTCCCTCCTGGTGAGCCGGGCCCACGAGAAGC
TTTTGGAGCACCTCCTCAAGCAGGAGGTGCCCGAGATCGCCGAGGGCATCGTGGAGATCAAGGCCATCGCCCGGGAGCCC
GGCCGCCGGAGCAAGGTGGCGGTCACGAGCCACAACCCCAACGTGGACCCCATCGGGGCCTGCATCGGGCACAAGGGCCA
GCGCATCCAGGCGGTTTCCGCCGAGCTCGGCCGGGAGAAGGTGGACATCATCCTGTGGTCCAAGGACCCCAAGGAGTTCA
TCCGCAACGCCCTCTCCCCCGCCCAGGTGGGCTCCATTGAGCTTGACCCCGAGGCGAAGAAGGCCCGGGTCAAGGTCACC
AAGGACCAGCACTCCCTGGCCATCGGCACCGGGGGGCAGAACGTCCGCCTGGCCTCCAAGCTCACCGGGTACGACATCCA
CTTTGAGGAGGCGGAGATCTCCGACCTGGACGAGGCCCTGCGGCGCGCCGCCGAGGAGGAGGCCGAGGCCCGGGAAAGGA
AGGCCCGCGAGGAGTTTGAGAAGCTCTTCCGTGACCTCGGATGA

Upstream 100 bases:

>100_bases
TTCCAGGTGGGCGACGAGGAGCGGCGCCTTAGGATCGGCACCTTCCGGGCCAACCTGGCGGAGTGGCCCGAGGAGCCCAG
GTAAAGGGAGGTAAAAAGCG

Downstream 100 bases:

>100_bases
AGCACGTCCCCATCCGCATGTGCGTGGCCTGCCGCCGGAGGCGGCCCAAGGGGGAGCTTCTCCGCATCCTGGTCACGGAG
GAGGGCTTCGTCATCGACCC

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MNREFVEAMQHLALERGVSVEEILEAFKEALRKAYIKRQKGYKKEEIDQGKGPEVDVYIDPNSGRIEMVEVRRVVEKVED
PDKEIALAEALQYDPEVQVGDEMEFPINPEELSRMAIQDLRQILTQRLKESERNRIYNEYKDKEGQVLTGVVTRVDNRGN
VFVELGRGEAYLPRSEQIPTEKYHPGQRIKVYLKKVDKSAKGPSLLVSRAHEKLLEHLLKQEVPEIAEGIVEIKAIAREP
GRRSKVAVTSHNPNVDPIGACIGHKGQRIQAVSAELGREKVDIILWSKDPKEFIRNALSPAQVGSIELDPEAKKARVKVT
KDQHSLAIGTGGQNVRLASKLTGYDIHFEEAEISDLDEALRRAAEEEAEARERKAREEFEKLFRDLG

Sequences:

>Translated_387_residues
MNREFVEAMQHLALERGVSVEEILEAFKEALRKAYIKRQKGYKKEEIDQGKGPEVDVYIDPNSGRIEMVEVRRVVEKVED
PDKEIALAEALQYDPEVQVGDEMEFPINPEELSRMAIQDLRQILTQRLKESERNRIYNEYKDKEGQVLTGVVTRVDNRGN
VFVELGRGEAYLPRSEQIPTEKYHPGQRIKVYLKKVDKSAKGPSLLVSRAHEKLLEHLLKQEVPEIAEGIVEIKAIAREP
GRRSKVAVTSHNPNVDPIGACIGHKGQRIQAVSAELGREKVDIILWSKDPKEFIRNALSPAQVGSIELDPEAKKARVKVT
KDQHSLAIGTGGQNVRLASKLTGYDIHFEEAEISDLDEALRRAAEEEAEARERKAREEFEKLFRDLG
>Mature_387_residues
MNREFVEAMQHLALERGVSVEEILEAFKEALRKAYIKRQKGYKKEEIDQGKGPEVDVYIDPNSGRIEMVEVRRVVEKVED
PDKEIALAEALQYDPEVQVGDEMEFPINPEELSRMAIQDLRQILTQRLKESERNRIYNEYKDKEGQVLTGVVTRVDNRGN
VFVELGRGEAYLPRSEQIPTEKYHPGQRIKVYLKKVDKSAKGPSLLVSRAHEKLLEHLLKQEVPEIAEGIVEIKAIAREP
GRRSKVAVTSHNPNVDPIGACIGHKGQRIQAVSAELGREKVDIILWSKDPKEFIRNALSPAQVGSIELDPEAKKARVKVT
KDQHSLAIGTGGQNVRLASKLTGYDIHFEEAEISDLDEALRRAAEEEAEARERKAREEFEKLFRDLG

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Escherichia coli, GI1789560, Length=348, Percent_Identity=38.2183908045977, Blast_Score=250, Evalue=1e-67,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NUSA_THET8 (P48514)

Other databases:

- EMBL:   Z48001
- EMBL:   AP008226
- PIR:   S52274
- RefSeq:   YP_143967.1
- ProteinModelPortal:   P48514
- SMR:   P48514
- STRING:   P48514
- GeneID:   3168103
- GenomeReviews:   AP008226_GR
- KEGG:   ttj:TTHA0701
- eggNOG:   COG0195
- HOGENOM:   HBG529269
- OMA:   MGSRVRA
- ProtClustDB:   PRK09202
- BioCyc:   TTHE300852:TTHA0701-MONOMER
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213
- Gene3D:   G3DSA:3.30.300.20
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.30.1480.10
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR01953

Pfam domain/function: PF08529 NusA_N; PF00575 S1; SSF54814 KH_prok; SSF50249 Nucleic_acid_OB; SSF69705 TF_NusA_N

EC number: NA

Molecular weight: Translated: 43940; Mature: 43940

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNREFVEAMQHLALERGVSVEEILEAFKEALRKAYIKRQKGYKKEEIDQGKGPEVDVYID
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCEEEEEEC
PNSGRIEMVEVRRVVEKVEDPDKEIALAEALQYDPEVQVGDEMEFPINPEELSRMAIQDL
CCCCCEEHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
RQILTQRLKESERNRIYNEYKDKEGQVLTGVVTRVDNRGNVFVELGRGEAYLPRSEQIPT
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHCCCCCCEEEEECCCCEECCCCCCCCC
EKYHPGQRIKVYLKKVDKSAKGPSLLVSRAHEKLLEHLLKQEVPEIAEGIVEIKAIAREP
CCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GRRSKVAVTSHNPNVDPIGACIGHKGQRIQAVSAELGREKVDIILWSKDPKEFIRNALSP
CCCCEEEEECCCCCCCHHHHHHCCCCCHHHHHHHHCCCCEEEEEEECCCHHHHHHHCCCC
AQVGSIELDPEAKKARVKVTKDQHSLAIGTGGQNVRLASKLTGYDIHFEEAEISDLDEAL
CCCCCEEECCCCCCCEEEEECCCCEEEEECCCCCEEEHHHCCCCEEEECCCHHHHHHHHH
RRAAEEEAEARERKAREEFEKLFRDLG
HHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNREFVEAMQHLALERGVSVEEILEAFKEALRKAYIKRQKGYKKEEIDQGKGPEVDVYID
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCEEEEEEC
PNSGRIEMVEVRRVVEKVEDPDKEIALAEALQYDPEVQVGDEMEFPINPEELSRMAIQDL
CCCCCEEHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
RQILTQRLKESERNRIYNEYKDKEGQVLTGVVTRVDNRGNVFVELGRGEAYLPRSEQIPT
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHCCCCCCEEEEECCCCEECCCCCCCCC
EKYHPGQRIKVYLKKVDKSAKGPSLLVSRAHEKLLEHLLKQEVPEIAEGIVEIKAIAREP
CCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GRRSKVAVTSHNPNVDPIGACIGHKGQRIQAVSAELGREKVDIILWSKDPKEFIRNALSP
CCCCEEEEECCCCCCCHHHHHHCCCCCHHHHHHHHCCCCEEEEEEECCCHHHHHHHCCCC
AQVGSIELDPEAKKARVKVTKDQHSLAIGTGGQNVRLASKLTGYDIHFEEAEISDLDEAL
CCCCCEEECCCCCCCEEEEECCCCEEEEECCCCCEEEHHHCCCCEEEECCCHHHHHHHHH
RRAAEEEAEARERKAREEFEKLFRDLG
HHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9242984