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The map label for this gene is rfbX [H]

Identifier: 55980618

GI number: 55980618

Start: 617153

End: 618418

Strand: Reverse

Name: rfbX [H]

Synonym: TTHA0649

Alternate gene names: 55980618

Gene position: 618418-617153 (Counterclockwise)

Preceding gene: 55980619

Following gene: 55980617

Centisome position: 33.43

GC content: 63.9

Gene sequence:

>1266_bases
GTGGGCCTCACCCCTCGCCTCTGGGCCAAGGTGGGAAGGGCGTGGCGGAGCCCCACCCTGCGCAACCTCTTTTACCTCTA
CGCTGTCCAGGCGGCCAACTACCTTTTCCCCCTCGTCACGTTGCCCTACTTGGCCCGCGTCCTGGGACCCGAGGGGTTTG
GCAAGCTCGCCCTGGCCCAGGCCTTGGTCCTTTACCTCTACGCTTTCTTGGAGTACGGCTACCAGTTTACCGCCACCCGT
GAGGTGGCCAGAAACCGGGAGGACCGAGCCGAGCTCGGTAAGATCGCGGCTGGCGTCCTCCACGCACGCCTTCTCCTTCT
TCTCCCCCTTCTTCCCCTCGGGATCGGGCTGGGGTGGGCGATCCCCCTACTTCGGGGGGACTTTTCCTTGACCGTAGGGG
CGCTCCTTGCCGCCATAGGGCAGGGCTTCAGCCCCCTCTGGTTCTTTCAGGGGCTGGAGCAGATGGGTAGGGTGGCCTTG
TTGGAAGTCCTCGTGCGGGGAGCCACGACCTTGGGCGTGTTTCTGCTGGTCCGAAGCCCCGGGGACGTGGCCGTACCCAT
CTATCTCCAGGCCGCTTCCTACTGGCTCACGGCCCTCCTGGGCCTGAGCTGGCTCCGTGCCGAGGTGGTATGGCCCGGGC
TTAGCGGAGGAGGGGCTTGGCTTCGCAAGGGGGTCACGTTTTTCGTGTACAACCTGGCCACACTCCTCTACACCAGCCTC
AACGTGACCTTGGTGAGCCTGGTGCTCCCGGCGGCCCAGGTAGGCCAGTACGCCGGGGCGGAGCGGCTGGTGCGGCCTTT
GGTGAACATGTGGTCGCCCATCACCCGTCTTTTCTTTCCCCGCCTAAGCTTCGCCTTCGCCCAGGGTAAACCCGAAGCCC
GGCGCTGGCTCTGGCAAGCCTTTTTGCTTACGCTGGGAGTGGGAGTGGTGCTGGGAGCAAGCCTGTTCCTCTCGGCCCCC
ATTTTAGTGTCCTGGTTCTTGGGCGCTGGTTATGAAATGGCCGTTCCCATCGTAAAAGTGCTTTCTCTGTTTCTAGTCCT
GAGTGCCCTTTCCAGCTTCGTGGGGATCCAACTCCTTCTGCCCATGGGTCTGGACCGCCCCTTCCTGGTCATTGCCCTTT
CGGCGGGCTTGCTAAACGTGGCCCTGGCCCTCTGGGCCCTTCCCCGGTGGGGGGTGCTGGCCATGCCATGGGTGGCGGTG
GTGGCGGAGGGCTGGGTAAGCCTAGGGATGCTCGGGGCTTGGCTCCTTCTTAAGTCTAGGAGGTAG

Upstream 100 bases:

>100_bases
GCACGGAGCCCGTGGTGCGGATCTACGTGGAGGCCCAAAGCCCCGAGCTCGTCCGGGCGCTTCTGGAGGAGGCGCGGAAG
CTCGTGGAGGGCTAGCGTCC

Downstream 100 bases:

>100_bases
AAGTCTATGCGTCCGGACGTAGCCTTTGCTCTCCCGGCGCTGGCTGGGGGAGGGGTGGAGCGGGTGTACCTGGACCTGGC
TGGGGCCATGGTGAAGGAGG

Product: O-antigen transporter

Products: lipopolysaccharide [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MGLTPRLWAKVGRAWRSPTLRNLFYLYAVQAANYLFPLVTLPYLARVLGPEGFGKLALAQALVLYLYAFLEYGYQFTATR
EVARNREDRAELGKIAAGVLHARLLLLLPLLPLGIGLGWAIPLLRGDFSLTVGALLAAIGQGFSPLWFFQGLEQMGRVAL
LEVLVRGATTLGVFLLVRSPGDVAVPIYLQAASYWLTALLGLSWLRAEVVWPGLSGGGAWLRKGVTFFVYNLATLLYTSL
NVTLVSLVLPAAQVGQYAGAERLVRPLVNMWSPITRLFFPRLSFAFAQGKPEARRWLWQAFLLTLGVGVVLGASLFLSAP
ILVSWFLGAGYEMAVPIVKVLSLFLVLSALSSFVGIQLLLPMGLDRPFLVIALSAGLLNVALALWALPRWGVLAMPWVAV
VAEGWVSLGMLGAWLLLKSRR

Sequences:

>Translated_421_residues
MGLTPRLWAKVGRAWRSPTLRNLFYLYAVQAANYLFPLVTLPYLARVLGPEGFGKLALAQALVLYLYAFLEYGYQFTATR
EVARNREDRAELGKIAAGVLHARLLLLLPLLPLGIGLGWAIPLLRGDFSLTVGALLAAIGQGFSPLWFFQGLEQMGRVAL
LEVLVRGATTLGVFLLVRSPGDVAVPIYLQAASYWLTALLGLSWLRAEVVWPGLSGGGAWLRKGVTFFVYNLATLLYTSL
NVTLVSLVLPAAQVGQYAGAERLVRPLVNMWSPITRLFFPRLSFAFAQGKPEARRWLWQAFLLTLGVGVVLGASLFLSAP
ILVSWFLGAGYEMAVPIVKVLSLFLVLSALSSFVGIQLLLPMGLDRPFLVIALSAGLLNVALALWALPRWGVLAMPWVAV
VAEGWVSLGMLGAWLLLKSRR
>Mature_420_residues
GLTPRLWAKVGRAWRSPTLRNLFYLYAVQAANYLFPLVTLPYLARVLGPEGFGKLALAQALVLYLYAFLEYGYQFTATRE
VARNREDRAELGKIAAGVLHARLLLLLPLLPLGIGLGWAIPLLRGDFSLTVGALLAAIGQGFSPLWFFQGLEQMGRVALL
EVLVRGATTLGVFLLVRSPGDVAVPIYLQAASYWLTALLGLSWLRAEVVWPGLSGGGAWLRKGVTFFVYNLATLLYTSLN
VTLVSLVLPAAQVGQYAGAERLVRPLVNMWSPITRLFFPRLSFAFAQGKPEARRWLWQAFLLTLGVGVVLGASLFLSAPI
LVSWFLGAGYEMAVPIVKVLSLFLVLSALSSFVGIQLLLPMGLDRPFLVIALSAGLLNVALALWALPRWGVLAMPWVAVV
AEGWVSLGMLGAWLLLKSRR

Specific function: May be involved in the translocation process of the nascent O-polysaccharide molecules and/or its ligation to lipid A core units [H]

COG id: COG2244

COG function: function code R; Membrane protein involved in the export of O-antigen and teichoic acid

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

Organism=Escherichia coli, GI1788349, Length=360, Percent_Identity=28.3333333333333, Blast_Score=154, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002797 [H]

Pfam domain/function: PF01943 Polysacc_synt [H]

EC number: NA

Molecular weight: Translated: 45990; Mature: 45858

Theoretical pI: Translated: 10.83; Mature: 10.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLTPRLWAKVGRAWRSPTLRNLFYLYAVQAANYLFPLVTLPYLARVLGPEGFGKLALAQ
CCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
ALVLYLYAFLEYGYQFTATREVARNREDRAELGKIAAGVLHARLLLLLPLLPLGIGLGWA
HHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IPLLRGDFSLTVGALLAAIGQGFSPLWFFQGLEQMGRVALLEVLVRGATTLGVFLLVRSP
HHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHEEEEECC
GDVAVPIYLQAASYWLTALLGLSWLRAEVVWPGLSGGGAWLRKGVTFFVYNLATLLYTSL
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
NVTLVSLVLPAAQVGQYAGAERLVRPLVNMWSPITRLFFPRLSFAFAQGKPEARRWLWQA
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
FLLTLGVGVVLGASLFLSAPILVSWFLGAGYEMAVPIVKVLSLFLVLSALSSFVGIQLLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PMGLDRPFLVIALSAGLLNVALALWALPRWGVLAMPWVAVVAEGWVSLGMLGAWLLLKSR
HCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
R
C
>Mature Secondary Structure 
GLTPRLWAKVGRAWRSPTLRNLFYLYAVQAANYLFPLVTLPYLARVLGPEGFGKLALAQ
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
ALVLYLYAFLEYGYQFTATREVARNREDRAELGKIAAGVLHARLLLLLPLLPLGIGLGWA
HHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IPLLRGDFSLTVGALLAAIGQGFSPLWFFQGLEQMGRVALLEVLVRGATTLGVFLLVRSP
HHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHEEEEECC
GDVAVPIYLQAASYWLTALLGLSWLRAEVVWPGLSGGGAWLRKGVTFFVYNLATLLYTSL
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
NVTLVSLVLPAAQVGQYAGAERLVRPLVNMWSPITRLFFPRLSFAFAQGKPEARRWLWQA
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
FLLTLGVGVVLGASLFLSAPILVSWFLGAGYEMAVPIVKVLSLFLVLSALSSFVGIQLLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PMGLDRPFLVIALSAGLLNVALALWALPRWGVLAMPWVAVVAEGWVSLGMLGAWLLLKSR
HCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
R
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lipopolysaccharide [Cytoplasm] [C]

Specific reaction: lipopolysaccharide [Cytoplasm] = lipopolysaccharide [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7517390; 7517391; 9097040; 9278503 [H]