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The map label for this gene is bioB [H]

Identifier: 55980576

GI number: 55980576

Start: 574551

End: 575540

Strand: Direct

Name: bioB [H]

Synonym: TTHA0607

Alternate gene names: 55980576

Gene position: 574551-575540 (Clockwise)

Preceding gene: 55980571

Following gene: 55980582

Centisome position: 31.06

GC content: 64.14

Gene sequence:

>990_bases
ATGGCGTGGCCCGATCTGGACCCTCCTCGCCTGGCCGAAAGGCCCTTGAGCCTACAGGAAGCCCTTTTTGTCCTCGAGGC
CCCACCCGAGGCCCTGCCTGCTTTGGCGGAGGCGGCCATCCGGGTCAAGGAGTACTTTTTCGGACGGCGGCTCAAGCTGG
TCCGTCTTCTCAACGTTAAAAGCGGCTTCTGCCCAGAGGACTGCGCCTACTGCGCCCAGTCCGCTAGATCCCAAGCCGCC
ATCGCCCGGTACCCCCTGCTCTCCTTGGAGGAGATCTTGGAGAGGGCCGAGGAGGCCCAACGCCTTTCTGCCCGCCGCTT
CTGCTTAGTGGCCGCCTTGCGGGGGCCTACCCCTAAGGTTTTGGAGCGCCTCGGTGAGGCCGCGCAAGCCATCAAGGCGC
GCTTTCCCCTTGAGCTTTGCGCCTCCTTAGGGCTCTTGGAAGAGGGTATGGCGGAGGCCTTAAAGGCGGCGGGTTTTGAC
TACTACAACCACAACCTCAACACCGCCCCAAGCCTTTACCCTAGGATCGCCACCACCCACACCTACCAGGACCGCTTGTG
GACCCTAAAGCGGGCTAGGGAAGCGGGGCTAAAGCTCTGTTCGGGGGTCATCCTGGGGATGGGGGAAGGTCCCAAGGAGG
TGTACGAGATGGCCTTGGCCCTGAGGGAGCTAGGGGTAGAGAGCCTTCCCGTGAACTTTCTCCTGCCCATCCCCGGCACC
CCCTTAGGGGATGGCCGCACGGTGGCGGGGCTTACCCCCGAGCGTGCCTTGAAGGCCCTGATCCTCTTCCGTCTTTTGAA
CCCCAAGGCCGAGCTCCGCGCCTCGGCGGGGCGGGAAAGGTATCTGGGCCCGTACGAGCCCCTGGCCTTCCGGATGGTGA
ACAGCATCTTCCTCCAGGGCTACCTTACCCAGCCGGGGAGCCCTTGGGAGCGGGACAGGGCCCTGTGGGAAAGCCTGGAC
CTTGATGTGGAGGAGGGAGCGTGCGGCTAA

Upstream 100 bases:

>100_bases
ATCACCCTCTTCACGAGGTCTAAGTTTAGGGCACGGCCGTGATTTGGGCAACGCGCCCCTTGCTTTCGTAAACTTTCTTA
GTTTATTTTGGTTTACGAAC

Downstream 100 bases:

>100_bases
TACCACCCCACGCTGGTCCAAAGCCTGCGGTGCGGGGCGCCTGGAGGCCCTAGGGGTTGGCGCCCGCCCGTGTGCCTAGG
TCGGCTTTGGCGGCGGGTCT

Product: biotin synthase (biotin synthetase)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MAWPDLDPPRLAERPLSLQEALFVLEAPPEALPALAEAAIRVKEYFFGRRLKLVRLLNVKSGFCPEDCAYCAQSARSQAA
IARYPLLSLEEILERAEEAQRLSARRFCLVAALRGPTPKVLERLGEAAQAIKARFPLELCASLGLLEEGMAEALKAAGFD
YYNHNLNTAPSLYPRIATTHTYQDRLWTLKRAREAGLKLCSGVILGMGEGPKEVYEMALALRELGVESLPVNFLLPIPGT
PLGDGRTVAGLTPERALKALILFRLLNPKAELRASAGRERYLGPYEPLAFRMVNSIFLQGYLTQPGSPWERDRALWESLD
LDVEEGACG

Sequences:

>Translated_329_residues
MAWPDLDPPRLAERPLSLQEALFVLEAPPEALPALAEAAIRVKEYFFGRRLKLVRLLNVKSGFCPEDCAYCAQSARSQAA
IARYPLLSLEEILERAEEAQRLSARRFCLVAALRGPTPKVLERLGEAAQAIKARFPLELCASLGLLEEGMAEALKAAGFD
YYNHNLNTAPSLYPRIATTHTYQDRLWTLKRAREAGLKLCSGVILGMGEGPKEVYEMALALRELGVESLPVNFLLPIPGT
PLGDGRTVAGLTPERALKALILFRLLNPKAELRASAGRERYLGPYEPLAFRMVNSIFLQGYLTQPGSPWERDRALWESLD
LDVEEGACG
>Mature_328_residues
AWPDLDPPRLAERPLSLQEALFVLEAPPEALPALAEAAIRVKEYFFGRRLKLVRLLNVKSGFCPEDCAYCAQSARSQAAI
ARYPLLSLEEILERAEEAQRLSARRFCLVAALRGPTPKVLERLGEAAQAIKARFPLELCASLGLLEEGMAEALKAAGFDY
YNHNLNTAPSLYPRIATTHTYQDRLWTLKRAREAGLKLCSGVILGMGEGPKEVYEMALALRELGVESLPVNFLLPIPGTP
LGDGRTVAGLTPERALKALILFRLLNPKAELRASAGRERYLGPYEPLAFRMVNSIFLQGYLTQPGSPWERDRALWESLDL
DVEEGACG

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=322, Percent_Identity=37.2670807453416, Blast_Score=202, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6321725, Length=288, Percent_Identity=34.7222222222222, Blast_Score=177, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 36389; Mature: 36257

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAWPDLDPPRLAERPLSLQEALFVLEAPPEALPALAEAAIRVKEYFFGRRLKLVRLLNVK
CCCCCCCCHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SGFCPEDCAYCAQSARSQAAIARYPLLSLEEILERAEEAQRLSARRFCLVAALRGPTPKV
CCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LERLGEAAQAIKARFPLELCASLGLLEEGMAEALKAAGFDYYNHNLNTAPSLYPRIATTH
HHHHHHHHHHHHHCCCHHHHHHHCHHHHHHHHHHHHHCCCHHCCCCCCCCCHHHHHHHCC
TYQDRLWTLKRAREAGLKLCSGVILGMGEGPKEVYEMALALRELGVESLPVNFLLPIPGT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCC
PLGDGRTVAGLTPERALKALILFRLLNPKAELRASAGRERYLGPYEPLAFRMVNSIFLQG
CCCCCCEECCCCHHHHHHHHHHHHHHCCHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHHH
YLTQPGSPWERDRALWESLDLDVEEGACG
HCCCCCCCHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
AWPDLDPPRLAERPLSLQEALFVLEAPPEALPALAEAAIRVKEYFFGRRLKLVRLLNVK
CCCCCCCHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SGFCPEDCAYCAQSARSQAAIARYPLLSLEEILERAEEAQRLSARRFCLVAALRGPTPKV
CCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LERLGEAAQAIKARFPLELCASLGLLEEGMAEALKAAGFDYYNHNLNTAPSLYPRIATTH
HHHHHHHHHHHHHCCCHHHHHHHCHHHHHHHHHHHHHCCCHHCCCCCCCCCHHHHHHHCC
TYQDRLWTLKRAREAGLKLCSGVILGMGEGPKEVYEMALALRELGVESLPVNFLLPIPGT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCC
PLGDGRTVAGLTPERALKALILFRLLNPKAELRASAGRERYLGPYEPLAFRMVNSIFLQG
CCCCCCEECCCCHHHHHHHHHHHHHHCCHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHHH
YLTQPGSPWERDRALWESLDLDVEEGACG
HCCCCCCCHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA