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The map label for this gene is mls [H]

Identifier: 55980475

GI number: 55980475

Start: 470584

End: 472146

Strand: Reverse

Name: mls [H]

Synonym: TTHA0506

Alternate gene names: 55980475

Gene position: 472146-470584 (Counterclockwise)

Preceding gene: 55980479

Following gene: 55980474

Centisome position: 25.52

GC content: 65.45

Gene sequence:

>1563_bases
ATGAAGGGCGTGGAGATCCGGAAAGACCACCCCCTCCTGAAGGAGGTCCTGACGGAGGAGGCCCTGAGGTTCGTGGTGGC
GCTGCACCGGGAGTTCAACCCGGTGCGCAAGGCCCTCCTGGAGCGGCGTCAAGCGCTTTGGGAGCGGTACAAGGCCGGGG
AGAAGCCGGACTTCCTCCAGGAGACCGCCTTCGTCCGGGGCGGGGCGTGGCGGGTGGCCGAGGCCCCGCCTGACCTCCTG
GACCGCCGGGTGGAGATCACGGGCCCCGTGGACCGCAAGATGATCATCAACGCCTTGAACTCGGGGGCCAAGGTCTTCAT
GGCGGACTTTGAGGACGCCCTCTCCCCCACCTGGGACAACGTCATCCGGGGCCAGAAGAACCTCTATGACGCCGTGCGCC
GCCAGATTGACTTCGTCTCCCCCGAGGGCAAGGAGTACAAGCTCAAGGAAAAGACGGCCACCCTGGTGGTGCGCCCAAGG
GGCTGGCACCTTGTGGAAAAGCACGTCTACGTGGACGGGGAGCCCATCTCGGCGAGCCTCTTTGACTTTGGCCTCTACTT
CTTCCACAACGCCCACGAGCTCTTAAGGCGGGGAAGCGGGCCCTACTTCTACCTGCCCAAGCTGGAAAGCCACCTCGAGG
CCCGCCTCTGGAACGAGGTCTTCAACTTCGCCCAGGACTACCTGAAGATCCCCCGGGGCACCATCCGGGCCACGGTCCTC
ATTGAGACCATCCTGGCGGCCTTTGAGATGGAGGAGATCCTCTACGAGCTCAAGGAGCACGCCGCCGGGCTCAACGCGGG
GCGCTGGGACTACATCTTCAGCTGCATCAAGAAGTTCGCCACCACCGCCCCCATCTTCCCCGACCGGGCCCAGGTGACGA
TGACCGTGCCCTTCATGAAGGCCTACACCGAGCTTTTGGTCAAGTCCTGCCACATCCACGAGGCCCACGCCATCGGCGGG
ATGGCGGCCTTCATCCCAAGCCGCAAGGACCCCCAGGTGAACGAGCGCGCCTTCCAGCAGGTGCGCGCGGACAAGGAACG
GGAGGCCTCCCAGGGCTTTGACGGCACCTGGGTGGCCCACCCCGACCTGGTGCCCGTGGCCATGGAGGTCTTTGACCGCT
ACCTGGGCGACAAGCCCCACCAGAAGCACGTGAAGCGGGAGGACGTCCACGTGACCGCGGCGGACCTCCTGAACTTCCAC
GTCCCCGAGGGGAGGGTGACGGAGGCGGGGCTTCGCAACAACATCTCCGTGGCCCTCCAGTACCTGAACCAGTGGCTCTT
GGGCAACGGGGCCGCGGCCATCTTCAACCTCATGGAGGACGCCGCCACCGCCGAGATCAGCCGGGCCCAGCTCTGGCAGT
GGGTGCACCGGGGGGCCCAGCTGGAGGACGGCCGCACCATTACCCCGGAGCTTTACCAAAAGGTCAAGGAGGAGGAGCTC
GCCAAGCTCGGCGGCCGGGACAAGGAGCGCTACCGGGAGGCCGAGGAGATCCTGGACAAGCTCGTCCTCTCCGAGGAGTT
CGTGGAGTTCCTGACCCTGGTGGCCTACGACTACATTGACTGA

Upstream 100 bases:

>100_bases
ATGCCGCTTAGGGTAACGGGGGCGGCCCTGTTTTTCAAGATACAAAAAATCTTTTTGCTTCTTGACAATCCCGCCCCGCC
TCCCGTAAGCTCGGACCACC

Downstream 100 bases:

>100_bases
GCCCCTCGCCGCGGTGGGGGCCCCCCTTTTGCCCTAAAGTGGAGGGGAAAGGAGGTCAAGGGTGGGCTTCTTGGAGGAAC
TCAAGGCCCTGCTTCCCGGC

Product: malate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 520; Mature: 520

Protein sequence:

>520_residues
MKGVEIRKDHPLLKEVLTEEALRFVVALHREFNPVRKALLERRQALWERYKAGEKPDFLQETAFVRGGAWRVAEAPPDLL
DRRVEITGPVDRKMIINALNSGAKVFMADFEDALSPTWDNVIRGQKNLYDAVRRQIDFVSPEGKEYKLKEKTATLVVRPR
GWHLVEKHVYVDGEPISASLFDFGLYFFHNAHELLRRGSGPYFYLPKLESHLEARLWNEVFNFAQDYLKIPRGTIRATVL
IETILAAFEMEEILYELKEHAAGLNAGRWDYIFSCIKKFATTAPIFPDRAQVTMTVPFMKAYTELLVKSCHIHEAHAIGG
MAAFIPSRKDPQVNERAFQQVRADKEREASQGFDGTWVAHPDLVPVAMEVFDRYLGDKPHQKHVKREDVHVTAADLLNFH
VPEGRVTEAGLRNNISVALQYLNQWLLGNGAAAIFNLMEDAATAEISRAQLWQWVHRGAQLEDGRTITPELYQKVKEEEL
AKLGGRDKERYREAEEILDKLVLSEEFVEFLTLVAYDYID

Sequences:

>Translated_520_residues
MKGVEIRKDHPLLKEVLTEEALRFVVALHREFNPVRKALLERRQALWERYKAGEKPDFLQETAFVRGGAWRVAEAPPDLL
DRRVEITGPVDRKMIINALNSGAKVFMADFEDALSPTWDNVIRGQKNLYDAVRRQIDFVSPEGKEYKLKEKTATLVVRPR
GWHLVEKHVYVDGEPISASLFDFGLYFFHNAHELLRRGSGPYFYLPKLESHLEARLWNEVFNFAQDYLKIPRGTIRATVL
IETILAAFEMEEILYELKEHAAGLNAGRWDYIFSCIKKFATTAPIFPDRAQVTMTVPFMKAYTELLVKSCHIHEAHAIGG
MAAFIPSRKDPQVNERAFQQVRADKEREASQGFDGTWVAHPDLVPVAMEVFDRYLGDKPHQKHVKREDVHVTAADLLNFH
VPEGRVTEAGLRNNISVALQYLNQWLLGNGAAAIFNLMEDAATAEISRAQLWQWVHRGAQLEDGRTITPELYQKVKEEEL
AKLGGRDKERYREAEEILDKLVLSEEFVEFLTLVAYDYID
>Mature_520_residues
MKGVEIRKDHPLLKEVLTEEALRFVVALHREFNPVRKALLERRQALWERYKAGEKPDFLQETAFVRGGAWRVAEAPPDLL
DRRVEITGPVDRKMIINALNSGAKVFMADFEDALSPTWDNVIRGQKNLYDAVRRQIDFVSPEGKEYKLKEKTATLVVRPR
GWHLVEKHVYVDGEPISASLFDFGLYFFHNAHELLRRGSGPYFYLPKLESHLEARLWNEVFNFAQDYLKIPRGTIRATVL
IETILAAFEMEEILYELKEHAAGLNAGRWDYIFSCIKKFATTAPIFPDRAQVTMTVPFMKAYTELLVKSCHIHEAHAIGG
MAAFIPSRKDPQVNERAFQQVRADKEREASQGFDGTWVAHPDLVPVAMEVFDRYLGDKPHQKHVKREDVHVTAADLLNFH
VPEGRVTEAGLRNNISVALQYLNQWLLGNGAAAIFNLMEDAATAEISRAQLWQWVHRGAQLEDGRTITPELYQKVKEEEL
AKLGGRDKERYREAEEILDKLVLSEEFVEFLTLVAYDYID

Specific function: Glyoxylate bypass; second step. [C]

COG id: COG2225

COG function: function code C; Malate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1790444, Length=512, Percent_Identity=50, Blast_Score=501, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI17561814, Length=518, Percent_Identity=53.8610038610039, Blast_Score=531, Evalue=1e-151,
Organism=Caenorhabditis elegans, GI71982926, Length=417, Percent_Identity=54.4364508393285, Blast_Score=440, Evalue=1e-123,
Organism=Saccharomyces cerevisiae, GI6324212, Length=516, Percent_Identity=53.2945736434108, Blast_Score=550, Evalue=1e-157,
Organism=Saccharomyces cerevisiae, GI6322222, Length=518, Percent_Identity=52.1235521235521, Blast_Score=539, Evalue=1e-154,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011076
- InterPro:   IPR006252
- InterPro:   IPR001465
- InterPro:   IPR019830 [H]

Pfam domain/function: PF01274 Malate_synthase [H]

EC number: =2.3.3.9 [H]

Molecular weight: Translated: 59729; Mature: 59729

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS00510 MALATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGVEIRKDHPLLKEVLTEEALRFVVALHREFNPVRKALLERRQALWERYKAGEKPDFLQ
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHH
ETAFVRGGAWRVAEAPPDLLDRRVEITGPVDRKMIINALNSGAKVFMADFEDALSPTWDN
HHHHHCCCCEEECCCCHHHHHCCEEECCCCHHHHHHHHHCCCCEEEEECHHHHCCCCHHH
VIRGQKNLYDAVRRQIDFVSPEGKEYKLKEKTATLVVRPRGWHLVEKHVYVDGEPISASL
HHCCHHHHHHHHHHHHHCCCCCCCCEEEECCCEEEEECCCCCEEEEEEEEECCCCCHHHH
FDFGLYFFHNAHELLRRGSGPYFYLPKLESHLEARLWNEVFNFAQDYLKIPRGTIRATVL
HHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
IETILAAFEMEEILYELKEHAAGLNAGRWDYIFSCIKKFATTAPIFPDRAQVTMTVPFMK
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEECHHHH
AYTELLVKSCHIHEAHAIGGMAAFIPSRKDPQVNERAFQQVRADKEREASQGFDGTWVAH
HHHHHHHHHHCCHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEECC
PDLVPVAMEVFDRYLGDKPHQKHVKREDVHVTAADLLNFHVPEGRVTEAGLRNNISVALQ
CCHHHHHHHHHHHHHCCCCHHHHCCHHCCEEEHHHHHHHCCCCCCCCHHHHHCHHHHHHH
YLNQWLLGNGAAAIFNLMEDAATAEISRAQLWQWVHRGAQLEDGRTITPELYQKVKEEEL
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
AKLGGRDKERYREAEEILDKLVLSEEFVEFLTLVAYDYID
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKGVEIRKDHPLLKEVLTEEALRFVVALHREFNPVRKALLERRQALWERYKAGEKPDFLQ
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHH
ETAFVRGGAWRVAEAPPDLLDRRVEITGPVDRKMIINALNSGAKVFMADFEDALSPTWDN
HHHHHCCCCEEECCCCHHHHHCCEEECCCCHHHHHHHHHCCCCEEEEECHHHHCCCCHHH
VIRGQKNLYDAVRRQIDFVSPEGKEYKLKEKTATLVVRPRGWHLVEKHVYVDGEPISASL
HHCCHHHHHHHHHHHHHCCCCCCCCEEEECCCEEEEECCCCCEEEEEEEEECCCCCHHHH
FDFGLYFFHNAHELLRRGSGPYFYLPKLESHLEARLWNEVFNFAQDYLKIPRGTIRATVL
HHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
IETILAAFEMEEILYELKEHAAGLNAGRWDYIFSCIKKFATTAPIFPDRAQVTMTVPFMK
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEECHHHH
AYTELLVKSCHIHEAHAIGGMAAFIPSRKDPQVNERAFQQVRADKEREASQGFDGTWVAH
HHHHHHHHHHCCHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEECC
PDLVPVAMEVFDRYLGDKPHQKHVKREDVHVTAADLLNFHVPEGRVTEAGLRNNISVALQ
CCHHHHHHHHHHHHHCCCCHHHHCCHHCCEEEHHHHHHHCCCCCCCCHHHHHCHHHHHHH
YLNQWLLGNGAAAIFNLMEDAATAEISRAQLWQWVHRGAQLEDGRTITPELYQKVKEEEL
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
AKLGGRDKERYREAEEILDKLVLSEEFVEFLTLVAYDYID
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA