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The map label for this gene is otsB [H]

Identifier: 55980448

GI number: 55980448

Start: 449475

End: 450179

Strand: Reverse

Name: otsB [H]

Synonym: TTHA0479

Alternate gene names: 55980448

Gene position: 450179-449475 (Counterclockwise)

Preceding gene: 55980449

Following gene: 55980447

Centisome position: 24.34

GC content: 70.21

Gene sequence:

>705_bases
ATGAGGGCGGAAAACCCCGTCTTCTTTCTGGACTACGACGGCACCCTGGCCCCCATCGCCCAAAGGCCCGAGGAGGCCTT
CCCCCACCCCGAGGCCCCCCGGGTCCTCCGGGCCCTCATGGAGCGCCACCCCGTCTACGTGGTCACGGGAAGAAGGGTGA
GGGACCTCGAGCCCCTCCTCCCCCTCCCGGGCCTTCCCGTGGTGGGGGGGCACGGCCTGGAGGAAGGGGTGCTCTTCGGG
GAGGTGCGGCCCCTCTTCCCCGTGGACCTTGGCCCCTTGCGGGCAAGGCTTCCCTCCTGCCCCGGGGTGCGGGTGGAGGA
CAAGGGCTTCGCCCTGGCCCTGCACTACCGGGGGGCGGAGGACGAGGAGAAGGCCCGGGCCTGCCTCGAGGCCTGGCTTA
AGGCGGTGGAGGGGCTCCTGGAAGCCTTGGGCCTCGAGGCCCTCCCCGGCAAGAAGGTCCTGGAGCTCAAGCCCAAGGGG
GTGGACAAGGGCCAAGCGGTCCTCAGGCTCCTCGGACGCCACCCGGACCACACCCCCGTTTACATCGGGGACGACACCAC
CGACGAGGCCGCCTTCCTCGCCTTAAGGGGCCGGGGCCTCACCTTCAAGGTGGGGGAAGGCCCCACGGCGGCCCAAGGCC
GGCTCAAGGACGTGGAGGAGGTCCTGGCCTACTTGCAAACCTACCTCCGACCCACTAGCCTTTAG

Upstream 100 bases:

>100_bases
GGGGGGAGCGGCGGGAACGGCTTGCCGCCTTGAAGGCCCGCATTGAGGCCTTGGACGTGCAGGGCTGGGCGGAGCGCTTC
CTCGCCTCCTTGGAGGAGGG

Downstream 100 bases:

>100_bases
GCCGTGGACCCCCTCTGGTACAAGGACGCGGTGATCTACCAGCTCCACGTCCGCTCCTTCTTTGACGCCAACAACGACGG
CTACGGGGACTTTGAGGGCC

Product: trehalose-6-phosphate phosphatase

Products: NA

Alternate protein names: TPP; Trehalose 6-phosphate phosphatase; Trehalose-phosphatase [H]

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MRAENPVFFLDYDGTLAPIAQRPEEAFPHPEAPRVLRALMERHPVYVVTGRRVRDLEPLLPLPGLPVVGGHGLEEGVLFG
EVRPLFPVDLGPLRARLPSCPGVRVEDKGFALALHYRGAEDEEKARACLEAWLKAVEGLLEALGLEALPGKKVLELKPKG
VDKGQAVLRLLGRHPDHTPVYIGDDTTDEAAFLALRGRGLTFKVGEGPTAAQGRLKDVEEVLAYLQTYLRPTSL

Sequences:

>Translated_234_residues
MRAENPVFFLDYDGTLAPIAQRPEEAFPHPEAPRVLRALMERHPVYVVTGRRVRDLEPLLPLPGLPVVGGHGLEEGVLFG
EVRPLFPVDLGPLRARLPSCPGVRVEDKGFALALHYRGAEDEEKARACLEAWLKAVEGLLEALGLEALPGKKVLELKPKG
VDKGQAVLRLLGRHPDHTPVYIGDDTTDEAAFLALRGRGLTFKVGEGPTAAQGRLKDVEEVLAYLQTYLRPTSL
>Mature_234_residues
MRAENPVFFLDYDGTLAPIAQRPEEAFPHPEAPRVLRALMERHPVYVVTGRRVRDLEPLLPLPGLPVVGGHGLEEGVLFG
EVRPLFPVDLGPLRARLPSCPGVRVEDKGFALALHYRGAEDEEKARACLEAWLKAVEGLLEALGLEALPGKKVLELKPKG
VDKGQAVLRLLGRHPDHTPVYIGDDTTDEAAFLALRGRGLTFKVGEGPTAAQGRLKDVEEVLAYLQTYLRPTSL

Specific function: Removes the phosphate from trehalose 6-phosphate to produce free trehalose [H]

COG id: COG1877

COG function: function code G; Trehalose-6-phosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trehalose phosphatase family [H]

Homologues:

Organism=Escherichia coli, GI1788207, Length=237, Percent_Identity=31.6455696202532, Blast_Score=96, Evalue=2e-21,
Organism=Drosophila melanogaster, GI20129309, Length=245, Percent_Identity=33.8775510204082, Blast_Score=103, Evalue=8e-23,
Organism=Drosophila melanogaster, GI19920676, Length=239, Percent_Identity=32.2175732217573, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI221473368, Length=226, Percent_Identity=29.2035398230088, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI20129307, Length=226, Percent_Identity=29.2035398230088, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24582490, Length=226, Percent_Identity=29.2035398230088, Blast_Score=82, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR003337 [H]

Pfam domain/function: PF02358 Trehalose_PPase [H]

EC number: =3.1.3.12 [H]

Molecular weight: Translated: 25626; Mature: 25626

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAENPVFFLDYDGTLAPIAQRPEEAFPHPEAPRVLRALMERHPVYVVTGRRVRDLEPLL
CCCCCCEEEEECCCCCCHHHCCCHHHCCCCCHHHHHHHHHHHCCEEEEECCCCCCCCCCC
PLPGLPVVGGHGLEEGVLFGEVRPLFPVDLGPLRARLPSCPGVRVEDKGFALALHYRGAE
CCCCCCEECCCCCCCCEEEECCCCCCCCCCCHHHHHCCCCCCCEEECCCEEEEEEECCCC
DEEKARACLEAWLKAVEGLLEALGLEALPGKKVLELKPKGVDKGQAVLRLLGRHPDHTPV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCE
YIGDDTTDEAAFLALRGRGLTFKVGEGPTAAQGRLKDVEEVLAYLQTYLRPTSL
EECCCCCCCCEEEEEECCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRAENPVFFLDYDGTLAPIAQRPEEAFPHPEAPRVLRALMERHPVYVVTGRRVRDLEPLL
CCCCCCEEEEECCCCCCHHHCCCHHHCCCCCHHHHHHHHHHHCCEEEEECCCCCCCCCCC
PLPGLPVVGGHGLEEGVLFGEVRPLFPVDLGPLRARLPSCPGVRVEDKGFALALHYRGAE
CCCCCCEECCCCCCCCEEEECCCCCCCCCCCHHHHHCCCCCCCEEECCCEEEEEEECCCC
DEEKARACLEAWLKAVEGLLEALGLEALPGKKVLELKPKGVDKGQAVLRLLGRHPDHTPV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCE
YIGDDTTDEAAFLALRGRGLTFKVGEGPTAAQGRLKDVEEVLAYLQTYLRPTSL
EECCCCCCCCEEEEEECCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]